ATE247708T1 - Verfahren zur regulierung der metabolischen stabilität von proteinen - Google Patents
Verfahren zur regulierung der metabolischen stabilität von proteinenInfo
- Publication number
- ATE247708T1 ATE247708T1 AT87907061T AT87907061T ATE247708T1 AT E247708 T1 ATE247708 T1 AT E247708T1 AT 87907061 T AT87907061 T AT 87907061T AT 87907061 T AT87907061 T AT 87907061T AT E247708 T1 ATE247708 T1 AT E247708T1
- Authority
- AT
- Austria
- Prior art keywords
- protein
- methods
- metabolic stability
- amino
- proteins
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
- C07K1/1075—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of amino acids or peptide residues
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US91515186A | 1986-10-02 | 1986-10-02 | |
PCT/US1987/002522 WO1988002406A2 (en) | 1986-10-02 | 1987-10-01 | Methods of regulating metabolic stability of proteins |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE247708T1 true ATE247708T1 (de) | 2003-09-15 |
Family
ID=25435312
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT87907061T ATE247708T1 (de) | 1986-10-02 | 1987-10-01 | Verfahren zur regulierung der metabolischen stabilität von proteinen |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0324789B1 (de) |
JP (4) | JP2612874B2 (de) |
AT (1) | ATE247708T1 (de) |
CA (1) | CA1313831C (de) |
DE (1) | DE3752372T2 (de) |
WO (1) | WO1988002406A2 (de) |
Families Citing this family (46)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5196321A (en) * | 1986-10-02 | 1993-03-23 | Massachusetts Institute Of Technology | Methods for in vitro cleavage of ubiquitin fusion proteins |
US5132213A (en) * | 1986-10-02 | 1992-07-21 | Massachusetts Institute Of Technology | Method for producing proteins and polypeptides using ubiquitin fusions |
US5108919A (en) * | 1988-06-24 | 1992-04-28 | Genentech, Inc. | Dna sequences encoding yeast ubiquitin hydrolase |
US5262322A (en) * | 1988-06-24 | 1993-11-16 | Genentech, Inc. | Host transformed with yeast gene and ubiquitin/polypeptide fusions |
US5156968A (en) * | 1988-06-24 | 1992-10-20 | Genentech, Inc. | Purified yeast ubiquitin hydrolase |
AU5963290A (en) * | 1989-06-30 | 1991-01-17 | Massachusetts Institute Of Technology | Inhibition of the n-end rule pathway in living cells |
US6068994A (en) * | 1989-08-07 | 2000-05-30 | Chiron Corporation | Ubiquitin expression system |
US5620923A (en) * | 1989-10-12 | 1997-04-15 | The University Of Utah | Synthesis of peptides as cloned ubiquitin extensions |
US5212058A (en) * | 1990-05-09 | 1993-05-18 | Massachusetts Institute Of Technology | Nucleic acid encoding ubiquitin-specific proteases |
DE69109109T2 (de) * | 1990-05-09 | 1995-09-14 | Massachusetts Inst Technology | Ubiquitinspezifische protease. |
ATE158816T1 (de) | 1990-11-26 | 1997-10-15 | Genetics Inst | Expression von pace in wirtszellen und verfahren zu dessen verwendung |
WO1992022655A1 (en) * | 1991-06-10 | 1992-12-23 | Lucky Limited | Hepatitis c diagnostics and vaccines |
US5656456A (en) * | 1992-07-13 | 1997-08-12 | Bionebraska, Inc. | Chemical method for selective modification of the N- and/or C-terminal amino acid α-carbon reactive group of a recombinant polypeptide or a portion thereof |
EP0651761B1 (de) * | 1992-07-13 | 2002-10-09 | Bionebraska, Inc. | Verfahren zur modifizierung rekombinanter polypeptide |
AU669256B2 (en) * | 1992-10-29 | 1996-05-30 | Celtrix Pharmaceuticals, Inc. | Uses of TGF-beta receptor fragment as a therapeutic agent |
US5459051A (en) * | 1993-03-26 | 1995-10-17 | Celtrix Pharmaceuticals, Inc. | Methods and vectors for over-expression of ubiquitin fusion proteins in host cells |
US5563046A (en) * | 1993-08-02 | 1996-10-08 | Celtrix Pharmaceuticals, Inc. | Fusion polypeptides and proteins |
US5914254A (en) * | 1993-08-02 | 1999-06-22 | Celtrix Pharmaceuticals, Inc. | Expression of fusion polypeptides transported out of the cytoplasm without leader sequences |
WO1996017941A2 (en) * | 1994-12-07 | 1996-06-13 | Bionebraska, Inc. | Production of c-terminal amidated peptides from recombinant protein constructs |
US6576469B1 (en) | 1997-09-10 | 2003-06-10 | President And Fellows Of Harvard College | Inducible methods for repressing gene function |
BR9813930A (pt) | 1997-11-06 | 2006-12-19 | Chiron Spa | antìgeno neisserial |
EP1047784B2 (de) | 1998-01-14 | 2015-03-18 | Novartis Vaccines and Diagnostics S.r.l. | Antigene aus neisseria meningitidis |
GB9808932D0 (en) | 1998-04-27 | 1998-06-24 | Chiron Spa | Polyepitope carrier protein |
NZ532665A (en) | 1998-05-01 | 2005-11-25 | Inst Genomic Research | Neisseria meningitidis antigens and compositions |
WO1999064583A2 (en) * | 1998-06-12 | 1999-12-16 | The Government Of The United States Of America, Represented By The Secretary, Department Of Healt H And Human Services | Compositions and methods for modulating proteolytic degradation of intracellular targets |
ATE360072T1 (de) * | 1998-07-10 | 2007-05-15 | Calgene Llc | Expression eukarotischer peptide in pflanzenplastiden |
WO2000042185A1 (en) * | 1999-01-11 | 2000-07-20 | Mindset Biopharmaceuticals (Usa), Inc. | Methods for regulating the stability of recombinant proteins and products useful therein |
NZ571167A (en) | 1999-04-30 | 2010-05-28 | Novartis Vaccines & Diagnostic | Fragments from Neisseria protein ORF 953 and their use in medicaments and diagnostic reagents |
GB9911683D0 (en) | 1999-05-19 | 1999-07-21 | Chiron Spa | Antigenic peptides |
GB9916529D0 (en) | 1999-07-14 | 1999-09-15 | Chiron Spa | Antigenic peptides |
EP2975127A1 (de) | 1999-10-29 | 2016-01-20 | GlaxoSmithKline Biologicals SA | Neisseria antigenpeptide |
EP2275129A3 (de) | 2000-01-17 | 2013-11-06 | Novartis Vaccines and Diagnostics S.r.l. | Ergänzter Außenmembranvesikel-Impfstoff gegen Meningokokken |
US7262005B1 (en) | 2000-02-04 | 2007-08-28 | Aurora Biosciences Corporation | Methods of protein destabilization and uses thereof |
PT1292697E (pt) * | 2000-06-15 | 2009-10-28 | Smithkline Beecham Corp | Método para a preparação de um polipéptido il-18 fisiologicamente activo |
SG165981A1 (en) | 2000-10-27 | 2010-11-29 | Chiron Srl | Nucleic acids and proteins from streptococcus groups a & b |
GB0107658D0 (en) | 2001-03-27 | 2001-05-16 | Chiron Spa | Streptococcus pneumoniae |
GB0107661D0 (en) | 2001-03-27 | 2001-05-16 | Chiron Spa | Staphylococcus aureus |
JPWO2002083907A1 (ja) * | 2001-04-10 | 2004-08-05 | 塩野義製薬株式会社 | UspAを用いた目的ポリペプチドの製造方法 |
EP2335723A1 (de) | 2001-12-12 | 2011-06-22 | Novartis Vaccines and Diagnostics S.r.l. | Immunisierung gegen Chlamydia trachomatis |
PT2279746E (pt) | 2002-11-15 | 2013-12-09 | Novartis Vaccines & Diagnostic | Proteínas de superfície de neisseria meningitidis |
GB0308198D0 (en) | 2003-04-09 | 2003-05-14 | Chiron Srl | ADP-ribosylating bacterial toxin |
ATE522541T1 (de) | 2006-06-09 | 2011-09-15 | Novartis Ag | Bakterielle adhäsine konformere |
KR101873201B1 (ko) | 2015-06-11 | 2018-07-02 | 주식회사 제넥신 | 변형된 인터루킨-7 단백질 및 이의 용도 |
KR102386735B1 (ko) | 2015-11-06 | 2022-04-14 | 주식회사 제넥신 | 변형된 인터루킨-7 융합 단백질의 제형 |
KR20170066265A (ko) | 2015-12-04 | 2017-06-14 | 주식회사 제넥신 | 면역글로불린 Fc가 융합된 인터루킨-7 융합 단백질을 포함하는 사람 파필로마바이러스 유래 질환의 예방 또는 치료용 약학적 조성물 |
US11357827B2 (en) | 2015-12-04 | 2022-06-14 | Genexine, Inc. | Method for preventing or treating influenza virus infection using pharmaceutical composition comprising immunoglobulin Fc-fused interleukin-7 fusion protein |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1985000831A1 (en) * | 1983-08-10 | 1985-02-28 | Amgen | Microbial expression of insulin-like growth factor |
EP0174999B1 (de) * | 1984-03-16 | 1991-04-17 | Biogen, Inc. | Verfahren zur Expressionsverbesserung und Mittel hierfür |
EP0163573B1 (de) * | 1984-05-24 | 1989-09-06 | Merck & Co. Inc. | Positionsspezifische Proteolyse mittels Renin |
ZA864555B (en) * | 1985-06-20 | 1987-11-25 | Monsanto Co | Release of peptides from polypeptides |
-
1987
- 1987-10-01 WO PCT/US1987/002522 patent/WO1988002406A2/en active IP Right Grant
- 1987-10-01 DE DE3752372T patent/DE3752372T2/de not_active Expired - Fee Related
- 1987-10-01 JP JP62506416A patent/JP2612874B2/ja not_active Expired - Fee Related
- 1987-10-01 AT AT87907061T patent/ATE247708T1/de not_active IP Right Cessation
- 1987-10-01 EP EP87907061A patent/EP0324789B1/de not_active Expired - Lifetime
- 1987-10-02 CA CA000548512A patent/CA1313831C/en not_active Expired - Fee Related
-
1996
- 1996-02-08 JP JP8045655A patent/JP2776414B2/ja not_active Expired - Lifetime
-
1997
- 1997-05-26 JP JP9149916A patent/JP2883594B2/ja not_active Expired - Lifetime
-
1998
- 1998-10-07 JP JP10299072A patent/JP3038193B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
WO1988002406A2 (en) | 1988-04-07 |
JP2776414B2 (ja) | 1998-07-16 |
JPH1057061A (ja) | 1998-03-03 |
JP3038193B2 (ja) | 2000-05-08 |
DE3752372D1 (de) | 2003-09-25 |
JP2612874B2 (ja) | 1997-05-21 |
EP0324789A1 (de) | 1989-07-26 |
CA1313831C (en) | 1993-02-23 |
JPH11187875A (ja) | 1999-07-13 |
JP2883594B2 (ja) | 1999-04-19 |
WO1988002406A3 (en) | 1988-07-14 |
DE3752372T2 (de) | 2004-06-24 |
EP0324789B1 (de) | 2003-08-20 |
JPH02501618A (ja) | 1990-06-07 |
JPH08252096A (ja) | 1996-10-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
UEP | Publication of translation of european patent specification |
Ref document number: 0324789 Country of ref document: EP |
|
REN | Ceased due to non-payment of the annual fee |