ATE355387T1 - Verfahren, vorrichtung und reagenziensatz zum zählen von bakterien - Google Patents
Verfahren, vorrichtung und reagenziensatz zum zählen von bakterienInfo
- Publication number
- ATE355387T1 ATE355387T1 AT03022247T AT03022247T ATE355387T1 AT E355387 T1 ATE355387 T1 AT E355387T1 AT 03022247 T AT03022247 T AT 03022247T AT 03022247 T AT03022247 T AT 03022247T AT E355387 T1 ATE355387 T1 AT E355387T1
- Authority
- AT
- Austria
- Prior art keywords
- bacteria
- counting
- assay sample
- optical information
- dead
- Prior art date
Links
- 241000894006 Bacteria Species 0.000 title abstract 12
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 2
- 238000003556 assay Methods 0.000 abstract 5
- 230000003287 optical effect Effects 0.000 abstract 4
- 239000007850 fluorescent dye Substances 0.000 abstract 3
- 238000001514 detection method Methods 0.000 abstract 2
- 238000010790 dilution Methods 0.000 abstract 2
- 239000012895 dilution Substances 0.000 abstract 2
- 239000012530 fluid Substances 0.000 abstract 2
- 238000010186 staining Methods 0.000 abstract 2
- 239000004094 surface-active agent Substances 0.000 abstract 2
- 238000004458 analytical method Methods 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 239000011800 void material Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2002292606A JP4236893B2 (ja) | 2002-10-04 | 2002-10-04 | 菌計数方法および菌計数装置 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE355387T1 true ATE355387T1 (de) | 2006-03-15 |
Family
ID=31987220
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT03022247T ATE355387T1 (de) | 2002-10-04 | 2003-10-01 | Verfahren, vorrichtung und reagenziensatz zum zählen von bakterien |
Country Status (5)
Country | Link |
---|---|
US (1) | US7422870B2 (de) |
EP (1) | EP1405918B1 (de) |
JP (1) | JP4236893B2 (de) |
AT (1) | ATE355387T1 (de) |
DE (1) | DE60312091T2 (de) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7309581B2 (en) * | 2000-11-01 | 2007-12-18 | Sysmex Corporation | Method of staining, detection and counting bacteria, and a diluent for bacterial stain |
JP2006067974A (ja) * | 2004-09-06 | 2006-03-16 | Sysmex Corp | 微生物の殺菌処理効果測定方法 |
JP4967280B2 (ja) * | 2005-08-30 | 2012-07-04 | パナソニック株式会社 | 微生物計数装置 |
JP4779518B2 (ja) * | 2005-09-08 | 2011-09-28 | パナソニック株式会社 | 蛍光読取装置および微生物計数装置 |
JP5140956B2 (ja) * | 2005-09-09 | 2013-02-13 | パナソニック株式会社 | 微生物計数装置 |
JP2007097582A (ja) * | 2005-09-08 | 2007-04-19 | Matsushita Electric Ind Co Ltd | 微生物計数装置 |
WO2007029821A1 (ja) * | 2005-09-08 | 2007-03-15 | Matsushita Electric Industrial Co., Ltd. | 微生物計数装置 |
JP4792880B2 (ja) * | 2005-09-08 | 2011-10-12 | パナソニック株式会社 | 微生物計数方法および微生物計数装置 |
JP4794334B2 (ja) * | 2006-03-22 | 2011-10-19 | シスメックス株式会社 | 尿試料分析用試薬及び尿試料の分析方法 |
JP4759438B2 (ja) * | 2006-05-17 | 2011-08-31 | シスメックス株式会社 | 尿中有形成分分析装置 |
JP2008187935A (ja) * | 2007-02-02 | 2008-08-21 | Matsushita Electric Ind Co Ltd | 微生物計数装置および点検用チップ |
US8148101B2 (en) | 2008-07-22 | 2012-04-03 | Abbott Laboratories | Method for classifying and counting bacteria in body fluids |
JP5480559B2 (ja) * | 2008-10-30 | 2014-04-23 | シスメックス株式会社 | 細菌分析装置、細菌分析方法及びコンピュータプログラム |
CN102019277B (zh) * | 2010-10-29 | 2013-05-22 | 北京惟馨雨生物科技有限公司 | 一种用于细胞和颗粒分离的分选仪及分选方法 |
EP2776836B1 (de) * | 2011-11-08 | 2018-01-24 | The Procter and Gamble Company | Verfahren zur identifizierung von präbiotika und diese enthaltende zusammensetzungen |
CA2884555C (en) * | 2012-10-08 | 2023-01-03 | Ventana Medical Systems, Inc. | Methods, kits, and systems for clarifying pigmented samples |
CN107805596A (zh) * | 2016-09-09 | 2018-03-16 | 东北农业大学 | 一种用于普通玻璃载玻片的细菌直接计数盒 |
BR112020018373A2 (pt) * | 2018-03-22 | 2020-12-29 | Idexx Laboratories, Inc. | Métodos de medição de bactérias em amostras biológicas |
CN110530827A (zh) * | 2019-07-16 | 2019-12-03 | 上海交通大学 | 一种粪菌定量的方法 |
CN112575054B (zh) * | 2019-12-16 | 2022-12-16 | 中国计量科学研究院 | 单增李斯特菌和细菌总数快速同步多重检测方法及试剂盒 |
WO2022241243A1 (en) * | 2021-05-13 | 2022-11-17 | Fluid-Screen, Inc. | Techniques for detection and quantification of live and dead bacteria in a fluid sample |
CN117642668A (zh) * | 2021-07-21 | 2024-03-01 | 索尼集团公司 | 物镜和样本分析装置 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4665024A (en) * | 1984-10-01 | 1987-05-12 | Becton, Dickinson And Company | Fluorescent gram stain |
SU1306947A1 (ru) * | 1985-07-24 | 1987-04-30 | Предприятие П/Я А-1631 | Способ определени концентрации живых микроорганизмов |
US5545535A (en) * | 1993-04-13 | 1996-08-13 | Molecular Probes, Inc. | Fluorescent assay for bacterial gram reaction |
JP3837006B2 (ja) | 2000-03-22 | 2006-10-25 | シスメックス株式会社 | 細菌の染色方法及び検出方法 |
US7309581B2 (en) * | 2000-11-01 | 2007-12-18 | Sysmex Corporation | Method of staining, detection and counting bacteria, and a diluent for bacterial stain |
US7585641B2 (en) * | 2003-01-21 | 2009-09-08 | Agdia, Inc. | Immunoassay and method of use |
JP4448673B2 (ja) * | 2003-08-22 | 2010-04-14 | シスメックス株式会社 | 細菌分析装置および方法 |
-
2002
- 2002-10-04 JP JP2002292606A patent/JP4236893B2/ja not_active Expired - Fee Related
-
2003
- 2003-10-01 AT AT03022247T patent/ATE355387T1/de not_active IP Right Cessation
- 2003-10-01 EP EP03022247A patent/EP1405918B1/de not_active Expired - Lifetime
- 2003-10-01 DE DE60312091T patent/DE60312091T2/de not_active Expired - Lifetime
- 2003-10-03 US US10/679,146 patent/US7422870B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
US20040067548A1 (en) | 2004-04-08 |
DE60312091T2 (de) | 2007-10-31 |
EP1405918A1 (de) | 2004-04-07 |
EP1405918B1 (de) | 2007-02-28 |
DE60312091D1 (de) | 2007-04-12 |
JP4236893B2 (ja) | 2009-03-11 |
JP2004121143A (ja) | 2004-04-22 |
US7422870B2 (en) | 2008-09-09 |
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Legal Events
Date | Code | Title | Description |
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RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |