ATE324465T1 - Nachweismethode mittels verstärkter silberfärbung - Google Patents

Nachweismethode mittels verstärkter silberfärbung

Info

Publication number
ATE324465T1
ATE324465T1 AT01954641T AT01954641T ATE324465T1 AT E324465 T1 ATE324465 T1 AT E324465T1 AT 01954641 T AT01954641 T AT 01954641T AT 01954641 T AT01954641 T AT 01954641T AT E324465 T1 ATE324465 T1 AT E324465T1
Authority
AT
Austria
Prior art keywords
tinting
detection method
silver
increased silver
increased
Prior art date
Application number
AT01954641T
Other languages
English (en)
Inventor
Robert L Letsinger
Viswanadham Garimella
Original Assignee
Univ Northwestern
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Northwestern filed Critical Univ Northwestern
Application granted granted Critical
Publication of ATE324465T1 publication Critical patent/ATE324465T1/de

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/137—Metal/ion, e.g. metal label
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/924—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis using nanostructure as support of dna analysis
AT01954641T 2000-07-11 2001-07-11 Nachweismethode mittels verstärkter silberfärbung ATE324465T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US21778200P 2000-07-11 2000-07-11

Publications (1)

Publication Number Publication Date
ATE324465T1 true ATE324465T1 (de) 2006-05-15

Family

ID=22812486

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01954641T ATE324465T1 (de) 2000-07-11 2001-07-11 Nachweismethode mittels verstärkter silberfärbung

Country Status (8)

Country Link
US (2) US6602669B2 (de)
EP (1) EP1360318B1 (de)
JP (1) JP3605607B2 (de)
AT (1) ATE324465T1 (de)
AU (2) AU7687001A (de)
CA (1) CA2415494C (de)
DE (1) DE60119170T2 (de)
WO (1) WO2002004681A2 (de)

Families Citing this family (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6582921B2 (en) 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US7169556B2 (en) * 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6767702B2 (en) 1996-07-29 2004-07-27 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6984491B2 (en) 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6506564B1 (en) * 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
EP1818417B1 (de) 1996-07-29 2014-02-12 Nanosphere, Inc. Nanopartikel mit angehängten Oligonukleotiden und ihre Verwendungen
US6750016B2 (en) 1996-07-29 2004-06-15 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US20050037397A1 (en) * 2001-03-28 2005-02-17 Nanosphere, Inc. Bio-barcode based detection of target analytes
US6974669B2 (en) * 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
IL126776A (en) * 1998-10-27 2001-04-30 Technion Res & Dev Foundation A method of investing gold
US7364920B2 (en) * 1999-10-27 2008-04-29 Technion Research And Development Foundation Ltd. Method for gold deposition
EP1360318B1 (de) 2000-07-11 2006-04-26 Northwestern University Nachweismethode mittels verstärkter silberfärbung
US20020160400A1 (en) * 2001-02-14 2002-10-31 Lakowicz Joseph R. Radiative decay engineering
US20060040286A1 (en) * 2001-03-28 2006-02-23 Nanosphere, Inc. Bio-barcode based detection of target analytes
US20030113740A1 (en) * 2001-04-26 2003-06-19 Mirkin Chad A. Oligonucleotide-modified ROMP polymers and co-polymers
US7147687B2 (en) * 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
WO2002096262A2 (en) * 2001-05-25 2002-12-05 Northwestern University Non-alloying core shell nanoparticles
CA2466656C (en) 2001-11-09 2013-07-02 Nanosphere, Inc. Bioconjugate-nanoparticle probes
GR1004178B (el) * 2001-11-29 2003-03-05 "����������" Ολοκληρωμενος οπτοηλεκτρονικος βιοαισθητηρας πυριτιου για ανιχνευση βιομοριων επισημασμενων με χρωμοφορες ομαδες ή νανοσωματιδια
EP1461596B1 (de) * 2001-11-30 2013-07-17 Northwestern University Direktes-schreiben-nanolithographische abscheidung von nukleinsäuren aus nanoskopischen spitzen
US7361310B1 (en) 2001-11-30 2008-04-22 Northwestern University Direct write nanolithographic deposition of nucleic acids from nanoscopic tips
US20030211488A1 (en) * 2002-05-07 2003-11-13 Northwestern University Nanoparticle probs with Raman spectrocopic fingerprints for analyte detection
EP1540006B1 (de) * 2002-07-02 2009-01-07 Nanosphere, Inc. Nanopartikel-polyanion-konjugate sowie verfahren zur verwendung davon beim nachweis von analyten
EP1387169B1 (de) * 2002-08-02 2006-05-24 Sony Deutschland GmbH Verfahren zur Bindung hydophiler Substanzen an hydrophile Makromoleküle und Immobilisierung derselben auf hydrophoben Oberflächen
US20040101859A1 (en) * 2002-11-25 2004-05-27 Cepheid Compositions, methods and kits for polynucleotide amplification reactions and microfluidic devices
EP1439392A3 (de) * 2003-01-15 2005-07-27 Agilent Technologies, Inc. Biosensorsysteme und Verfahren zur Detektion der Anwesenheit von Biomolekülen
WO2004074508A2 (en) * 2003-02-15 2004-09-02 Golovlev Valeri V Method of visualization and quantification of biopolymer molecules immobilized on solid support
US20080050842A1 (en) * 2003-02-15 2008-02-28 Golovlev Valeri V Method of visualization and quanitification of biopolymer molecules immobilized on solid support
WO2005001143A2 (en) * 2003-02-27 2005-01-06 Nanosphere, Inc. Label-free gene expression profiling with universal nanoparticle probes in microarray assay format
EP1634050A4 (de) * 2003-05-16 2008-06-18 Univ Rochester Kolorimetrische und fluoreszenzverfahren zur wahrnehmung von oligonukleotiden
US20060166249A1 (en) * 2003-05-16 2006-07-27 University Of Rochester Methods for separating short single-stranded nucleic acid from long single-and double-stranded nucleic acid, and associated biomolecular assays
US20050250094A1 (en) * 2003-05-30 2005-11-10 Nanosphere, Inc. Method for detecting analytes based on evanescent illumination and scatter-based detection of nanoparticle probe complexes
US7354706B2 (en) * 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
US20090163375A1 (en) 2003-09-09 2009-06-25 Bowman Christopher N Use of Photopolymerization for Amplification and Detection of a Molecular Recognition Event
CA2834041C (en) 2003-12-31 2017-05-16 President And Fellows Of Harvard College Assay device and method
SE0401033D0 (sv) * 2004-04-20 2004-04-20 Amersham Biosciences Ab Device and method for protein analysis
CA2572178A1 (en) * 2004-07-26 2006-03-16 Nanosphere, Inc. Method for distinguishing methicillin resistant s. aureus from methicillin sensitive s. aureus in a mixed culture
US20060068398A1 (en) * 2004-09-24 2006-03-30 Cepheid Universal and target specific reagent beads for nucleic acid amplification
EP1885877A2 (de) * 2005-02-17 2008-02-13 Istituto Nazionale per le Malattie Infettive "Lazzaro Spallanzani" IRCCS Zusammensetzungen und verfahren zum nachweis von für krankheitserreger spezifischen nukleinsäuren in urin
US8628918B2 (en) * 2005-05-09 2014-01-14 Affymetrix, Inc. Multiplex capture of nucleic acids
WO2006125124A2 (en) * 2005-05-18 2006-11-23 Nanosphere, Inc. Substrate functionalization method for high sensitivity applications
US8252756B2 (en) 2005-06-14 2012-08-28 Northwestern University Nucleic acid functionalized nanoparticles for therapeutic applications
US20070154903A1 (en) * 2005-06-23 2007-07-05 Nanosphere, Inc. Selective isolation and concentration of nucleic acids from complex samples
JP2009505106A (ja) * 2005-08-19 2009-02-05 ナノスフェアー インコーポレイテッド Ｄｎａ及び抗体を含むハイブリッド基板を調製するための方法及びその使用
US20090280188A1 (en) * 2006-06-23 2009-11-12 Northwestern University Asymmetric functionalizated nanoparticles and methods of use
CN103966345A (zh) 2007-02-09 2014-08-06 西北大学 检测细胞内标靶的颗粒
CA2679586A1 (en) * 2007-02-27 2008-10-23 Northwestern University Molecule attachment to nanoparticles
DK2156185T3 (da) * 2007-06-01 2013-11-18 Atonomics As Bio surface acoustic wave (saw) resonator-amplifikation med nanopartikler til påvisning af en målsat analyt
KR100898623B1 (ko) * 2007-06-27 2009-05-21 한국과학기술원 금 나노입자를 이용하여 바이오물질을 기판에 고정화시키는방법
EP2205735B1 (de) * 2007-09-16 2016-04-13 Instytut Hodowli i Aklimatyzacji Roslin - Panstwowy Instytut Badawczy Immunologische testverfahren auf das vorliegen von bakterien unter verwendung von mit einem spezifischen verfahren gewonnenen antikörpern
JP5091075B2 (ja) * 2007-09-28 2012-12-05 富士フイルム株式会社 イムノクロマトグラフ方法
US20090137405A1 (en) * 2007-11-16 2009-05-28 Christopher Bowman Detection of nucleic acid biomarkers using polymerization-based amplification
JP4980946B2 (ja) * 2008-02-12 2012-07-18 富士フイルム株式会社 ブロッティング検出方法
US8609433B2 (en) 2009-12-04 2013-12-17 Rapid Pathogen Screening, Inc. Multiplanar lateral flow assay with sample compressor
US20130196310A1 (en) 2008-05-20 2013-08-01 Rapid Pathogen Screening, Inc. Method and Device for Combined Detection of Viral and Bacterial Infections
US8962260B2 (en) 2008-05-20 2015-02-24 Rapid Pathogen Screening, Inc. Method and device for combined detection of viral and bacterial infections
US9068981B2 (en) 2009-12-04 2015-06-30 Rapid Pathogen Screening, Inc. Lateral flow assays with time delayed components
US8815609B2 (en) 2008-05-20 2014-08-26 Rapid Pathogen Screening, Inc. Multiplanar lateral flow assay with diverting zone
KR101692880B1 (ko) 2008-11-24 2017-01-04 노오쓰웨스턴 유니버시티 다가 ｒｎａ-나노입자 구조체
US20100233270A1 (en) 2009-01-08 2010-09-16 Northwestern University Delivery of Oligonucleotide-Functionalized Nanoparticles
KR20120136345A (ko) 2009-10-30 2012-12-18 노오쓰웨스턴 유니버시티 주형화된 나노컨쥬게이트
CA2805720A1 (en) 2010-06-17 2011-12-22 Abaxis, Inc. Rotors for immunoassays
CN102226811B (zh) * 2011-04-02 2013-10-23 长春市大格药物研究所 一种检测尿液中心脏型脂肪酸结合蛋白诊断试纸的制备方法
US9605304B2 (en) * 2011-07-20 2017-03-28 The Hong Kong Polytechnic University Ultra-stable oligonucleotide-gold and-silver nanoparticle conjugates and method of their preparation
AU2012308302A1 (en) 2011-09-14 2014-03-20 Northwestern University Nanoconjugates able to cross the blood-brain barrier
US9051583B2 (en) 2011-12-19 2015-06-09 Northwestern University Modified silica shell particles, and methods of making and using the same
CN102759526B (zh) * 2012-06-28 2014-08-27 宁波大学 一种金标银染定量检测汞离子的方法及其试剂盒
AU2014292926B2 (en) 2013-07-25 2020-03-05 Exicure Operating Company Spherical nucleic acid-based constructs as immunostimulatory agents for prophylactic and therapeutic use
AU2015349680A1 (en) 2014-11-21 2017-06-08 Northwestern University The sequence-specific cellular uptake of spherical nucleic acid nanoparticle conjugates
US10808287B2 (en) 2015-10-23 2020-10-20 Rapid Pathogen Screening, Inc. Methods and devices for accurate diagnosis of infections
EP3387154A4 (de) * 2015-12-09 2019-05-15 Intuitive Biosciences, Inc. Automatisiertes silberveredelungssystem
US11300386B2 (en) 2015-12-31 2022-04-12 Dupont Safety & Construction, Inc. Ballistic materials incorporating spunlaced nonwovens
WO2018039629A2 (en) 2016-08-25 2018-03-01 Northwestern University Micellar spherical nucleic acids from thermoresponsive, traceless templates
US11433131B2 (en) 2017-05-11 2022-09-06 Northwestern University Adoptive cell therapy using spherical nucleic acids (SNAs)
EP3820610A1 (de) 2018-07-12 2021-05-19 Luminex Corporation Systeme und verfahren zur durchführung variabler probenvorbereitung und analyseverfahren

Family Cites Families (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4193983A (en) 1978-05-16 1980-03-18 Syva Company Labeled liposome particle compositions and immunoassays therewith
NL7807532A (nl) 1978-07-13 1980-01-15 Akzo Nv Metaal-immunotest.
US4318707A (en) 1978-11-24 1982-03-09 Syva Company Macromolecular fluorescent quencher particle in specific receptor assays
US4256834A (en) 1979-04-09 1981-03-17 Syva Company Fluorescent scavenger particle immunoassay
US4261968A (en) 1979-05-10 1981-04-14 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4650770A (en) 1981-04-27 1987-03-17 Syntex (U.S.A.) Inc. Energy absorbing particle quenching in light emitting competitive protein binding assays
US4713348A (en) 1983-04-05 1987-12-15 Syntex (U.S.A.) Inc. Fluorescent multiparameter particle analysis
US5288609A (en) 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
US4868104A (en) 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US4996143A (en) * 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US5137827A (en) 1986-03-25 1992-08-11 Midwest Research Technologies, Inc. Diagnostic element for electrical detection of a binding reaction
US5514602A (en) 1986-06-09 1996-05-07 Ortho Diagnostic Systems, Inc. Method of producing a metal sol reagent containing colloidal metal particles
US5360895A (en) 1987-04-22 1994-11-01 Associated Universities, Inc. Derivatized gold clusters and antibody-gold cluster conjugates
US4853335A (en) 1987-09-28 1989-08-01 Olsen Duane A Colloidal gold particle concentration immunoassay
GB8800702D0 (en) 1988-01-13 1988-02-10 Nycomed As Test method & reagent kit therefor
WO1990002205A1 (en) 1988-08-25 1990-03-08 Angenics, Inc. Detection of nucleic acid sequences using particle agglutination
US5460831A (en) 1990-06-22 1995-10-24 The Regents Of The University Of California Targeted transfection nanoparticles
GB9019512D0 (en) 1990-09-06 1990-10-24 Ici Plc Assay method
US5665582A (en) 1990-10-29 1997-09-09 Dekalb Genetics Corp. Isolation of biological materials
JPH06510363A (ja) * 1990-10-29 1994-11-17 ディカルブ　プラント　ジェネティクス 磁気性粒子を使用する生物学的材料の単離
US5294369A (en) 1990-12-05 1994-03-15 Akzo N.V. Ligand gold bonding
EP0613585A4 (de) 1991-11-22 1995-06-21 Univ California Halbleitende nanokristalle, die durch selbstzusammengesetzte einzelschichten mit festen anorganischen oberflüchen verbunden sind.
US5225064A (en) 1992-01-15 1993-07-06 Enzyme Technology Research Group, Inc. Peroxidase colloidal gold oxidase biosensors for mediatorless glucose determination
GB9212164D0 (en) 1992-06-09 1992-07-22 Medical Res Council Preparation of nucleic acids
US5472881A (en) 1992-11-12 1995-12-05 University Of Utah Research Foundation Thiol labeling of DNA for attachment to gold surfaces
US5384265A (en) 1993-03-26 1995-01-24 Geo-Centers, Inc. Biomolecules bound to catalytic inorganic particles, immunoassays using the same
US5637508A (en) 1993-03-26 1997-06-10 Geo-Centers, Inc. Biomolecules bound to polymer or copolymer coated catalytic inorganic particles, immunoassays using the same and kits containing the same
US5681943A (en) 1993-04-12 1997-10-28 Northwestern University Method for covalently linking adjacent oligonucleotides
EP0630974A3 (de) 1993-06-25 1995-11-15 Clinical Diagnostic Syst Verfahren und Testkit zum Nachweis von anorganischen Orthophosphat bei der Amplifizierung von Zielnukleinsäuren.
US5543158A (en) 1993-07-23 1996-08-06 Massachusetts Institute Of Technology Biodegradable injectable nanoparticles
JPH07227299A (ja) 1994-02-14 1995-08-29 Kyoto Daiichi Kagaku:Kk Ｄｎａの特定塩基配列の検出方法及びその装置
US5521289A (en) 1994-07-29 1996-05-28 Nanoprobes, Inc. Small organometallic probes
US5599668A (en) 1994-09-22 1997-02-04 Abbott Laboratories Light scattering optical waveguide method for detecting specific binding events
US5609907A (en) 1995-02-09 1997-03-11 The Penn State Research Foundation Self-assembled metal colloid monolayers
US6025202A (en) 1995-02-09 2000-02-15 The Penn State Research Foundation Self-assembled metal colloid monolayers and detection methods therewith
JP2000510582A (ja) * 1996-04-25 2000-08-15 ゼニコン・サイエンシーズ・コーポレーション 微粒子標識を使用した分析物アッセイ
US6586193B2 (en) 1996-04-25 2003-07-01 Genicon Sciences Corporation Analyte assay using particulate labels
US6984491B2 (en) * 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6361944B1 (en) 1996-07-29 2002-03-26 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6767702B2 (en) * 1996-07-29 2004-07-27 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6750016B2 (en) * 1996-07-29 2004-06-15 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
EP1818417B1 (de) 1996-07-29 2014-02-12 Nanosphere, Inc. Nanopartikel mit angehängten Oligonukleotiden und ihre Verwendungen
US7169556B2 (en) * 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6506564B1 (en) * 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6582921B2 (en) * 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US6242264B1 (en) 1996-09-04 2001-06-05 The Penn State Research Foundation Self-assembled metal colloid monolayers having size and density gradients
DE59707744D1 (de) 1996-10-23 2002-08-22 Sueddeutsche Kalkstickstoff Verfahren zur herstellung von biologisch aktiven polymernanopartikel-nucleinsäure-konjugaten
US5830986A (en) 1996-10-28 1998-11-03 Massachusetts Institute Of Technology Methods for the synthesis of functionalizable poly(ethylene oxide) star macromolecules
US5900481A (en) 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
US5922537A (en) * 1996-11-08 1999-07-13 N.o slashed.AB Immunoassay, Inc. Nanoparticles biosensor
US5939021A (en) 1997-01-23 1999-08-17 Hansen; W. Peter Homogeneous binding assay
US6974669B2 (en) * 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US6149868A (en) 1997-10-28 2000-11-21 The Penn State Research Foundation Surface enhanced raman scattering from metal nanoparticle-analyte-noble metal substrate sandwiches
AUPP004497A0 (en) 1997-10-28 1997-11-20 University Of Melbourne, The Stabilized particles
AU1450699A (en) 1997-10-31 1999-05-24 Gen-Probe Incorporated Methods of nucleic acid detection
US5990479A (en) 1997-11-25 1999-11-23 Regents Of The University Of California Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes
US5972615A (en) * 1998-01-21 1999-10-26 Urocor, Inc. Biomarkers and targets for diagnosis, prognosis and management of prostate disease
US6287765B1 (en) 1998-05-20 2001-09-11 Molecular Machines, Inc. Methods for detecting and identifying single molecules
US6290839B1 (en) * 1998-06-23 2001-09-18 Clinical Micro Sensors, Inc. Systems for electrophoretic transport and detection of analytes
US6406921B1 (en) 1998-07-14 2002-06-18 Zyomyx, Incorporated Protein arrays for high-throughput screening
US6251303B1 (en) 1998-09-18 2001-06-26 Massachusetts Institute Of Technology Water-soluble fluorescent nanocrystals
US6306610B1 (en) 1998-09-18 2001-10-23 Massachusetts Institute Of Technology Biological applications of quantum dots
US6203989B1 (en) 1998-09-30 2001-03-20 Affymetrix, Inc. Methods and compositions for amplifying detectable signals in specific binding assays
DE69936534T2 (de) 1998-11-30 2008-03-13 Nanosphere, Inc., Northbrook Nanopartikel mit polymerschalen
US6277489B1 (en) 1998-12-04 2001-08-21 The Regents Of The University Of California Support for high performance affinity chromatography and other uses
JP2003503699A (ja) 1999-06-25 2003-01-28 ナノスフェアー インコーポレイテッド オリゴヌクレオチドが付着しているナノ粒子およびその利用法
ATE487136T1 (de) 2000-03-28 2010-11-15 Nanosphere Inc Nanopartikel mit gebundenen oligonukleotiden und verwendungen derselben
WO2001086301A1 (fr) 2000-05-11 2001-11-15 Center For Advanced Science And Technology Incubation, Ltd. Composition polymère pour former la surface d'un biocapteur
EP1360318B1 (de) 2000-07-11 2006-04-26 Northwestern University Nachweismethode mittels verstärkter silberfärbung
EP1362121A2 (de) 2000-08-11 2003-11-19 Nanosphere, Inc. Nanopartikel verbunden mit oligonukleotiden und verwendungen davon
ES2382636T3 (es) 2000-10-31 2012-06-12 Surmodics Pharmaceuticals, Inc. Método para producir composiciones para la administración mejorada de moléculas bioactivas
US6726847B2 (en) * 2000-12-06 2004-04-27 Northwestern University Silver stain removal by chemical etching and sonication
AU2002230593A1 (en) 2000-12-08 2002-06-18 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US20030113740A1 (en) * 2001-04-26 2003-06-19 Mirkin Chad A. Oligonucleotide-modified ROMP polymers and co-polymers
WO2002096262A2 (en) * 2001-05-25 2002-12-05 Northwestern University Non-alloying core shell nanoparticles
US7147687B2 (en) * 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
DE60231309D1 (de) * 2001-08-03 2009-04-09 Nanosphere Inc Nanopartikelabbildungssystem und -verfahren
CA2466656C (en) * 2001-11-09 2013-07-02 Nanosphere, Inc. Bioconjugate-nanoparticle probes
US20030211488A1 (en) * 2002-05-07 2003-11-13 Northwestern University Nanoparticle probs with Raman spectrocopic fingerprints for analyte detection
EP1540006B1 (de) * 2002-07-02 2009-01-07 Nanosphere, Inc. Nanopartikel-polyanion-konjugate sowie verfahren zur verwendung davon beim nachweis von analyten

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CA2415494A1 (en) 2002-01-17
US20020034756A1 (en) 2002-03-21
DE60119170T2 (de) 2007-05-24
WO2002004681A2 (en) 2002-01-17
WO2002004681A3 (en) 2003-08-07
EP1360318B1 (de) 2006-04-26
EP1360318A2 (de) 2003-11-12
JP2004502950A (ja) 2004-01-29
JP3605607B2 (ja) 2004-12-22
US6602669B2 (en) 2003-08-05
CA2415494C (en) 2007-09-18
DE60119170D1 (de) 2006-06-01
AU2001276870B2 (en) 2006-07-20
AU7687001A (en) 2002-01-21
US20040101889A1 (en) 2004-05-27

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