ATE312946T1 - Verfahren und vorrichtung zur bestimmung der gewebespezifität von freier dna in körperflüssigkeiten - Google Patents

Verfahren und vorrichtung zur bestimmung der gewebespezifität von freier dna in körperflüssigkeiten

Info

Publication number
ATE312946T1
ATE312946T1 AT02004954T AT02004954T ATE312946T1 AT E312946 T1 ATE312946 T1 AT E312946T1 AT 02004954 T AT02004954 T AT 02004954T AT 02004954 T AT02004954 T AT 02004954T AT E312946 T1 ATE312946 T1 AT E312946T1
Authority
AT
Austria
Prior art keywords
determining
amount
free floating
dna
floating dna
Prior art date
Application number
AT02004954T
Other languages
English (en)
Inventor
Kurt Berlin
Andrzej Sledziewski
Original Assignee
Epigenomics Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics Ag filed Critical Epigenomics Ag
Application granted granted Critical
Publication of ATE312946T1 publication Critical patent/ATE312946T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT02004954T 2002-03-05 2002-03-05 Verfahren und vorrichtung zur bestimmung der gewebespezifität von freier dna in körperflüssigkeiten ATE312946T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP02004954A EP1342794B1 (de) 2002-03-05 2002-03-05 Verfahren und Vorrichtung zur Bestimmung der Gewebespezifität von freier DNA in Körperflüssigkeiten

Publications (1)

Publication Number Publication Date
ATE312946T1 true ATE312946T1 (de) 2005-12-15

Family

ID=27741132

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02004954T ATE312946T1 (de) 2002-03-05 2002-03-05 Verfahren und vorrichtung zur bestimmung der gewebespezifität von freier dna in körperflüssigkeiten

Country Status (10)

Country Link
US (1) US20050221314A1 (de)
EP (2) EP1342794B1 (de)
JP (1) JP4727149B2 (de)
AT (1) ATE312946T1 (de)
AU (1) AU2003218691B2 (de)
CA (1) CA2478493A1 (de)
DE (1) DE60207979T2 (de)
DK (1) DK1342794T3 (de)
ES (1) ES2253461T3 (de)
WO (1) WO2003074730A1 (de)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060183128A1 (en) * 2003-08-12 2006-08-17 Epigenomics Ag Methods and compositions for differentiating tissues for cell types using epigenetic markers
DE10348407A1 (de) * 2003-10-17 2005-05-19 Widschwendter, Martin, Prof. Prognostische und diagnostische Marker für Zell-proliferative Erkrankungen von Brustgeweben
WO2007057231A1 (en) * 2005-11-17 2007-05-24 Epigenomics Ag Method for the determination of the dna methylation level of a cpg position in identical cells within a tissue sample
US20100124743A1 (en) * 2007-01-23 2010-05-20 Olympus Corporation Method for diagnosis of cancer
JP5378687B2 (ja) * 2007-03-02 2013-12-25 エフ.ホフマン−ラ ロシュ アーゲー Basp1遺伝子および/またはsrd5a2遺伝子中のメチル化シトシンを利用する、肝臓癌、肝臓癌発症リスク、肝臓癌再発リスク、肝臓癌悪性度および肝臓癌の経時的進展の検出方法
WO2009003654A2 (en) * 2007-06-29 2009-01-08 Medizinische Universität Innsbruck Selecting nucleic acid samples suitable for genotyping
US20100112590A1 (en) 2007-07-23 2010-05-06 The Chinese University Of Hong Kong Diagnosing Fetal Chromosomal Aneuploidy Using Genomic Sequencing With Enrichment
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
WO2014039556A1 (en) 2012-09-04 2014-03-13 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
LT3354747T (lt) 2012-09-20 2021-04-12 The Chinese University Of Hong Kong Neinvazinis naviko metilomos nustatymas iš plazmos
US10706957B2 (en) 2012-09-20 2020-07-07 The Chinese University Of Hong Kong Non-invasive determination of methylome of tumor from plasma
US9732390B2 (en) 2012-09-20 2017-08-15 The Chinese University Of Hong Kong Non-invasive determination of methylome of fetus or tumor from plasma
US9128861B2 (en) 2013-01-17 2015-09-08 Personalis, Inc. Methods and systems for genetic analysis
EP3965111A1 (de) 2013-08-30 2022-03-09 Personalis, Inc. Verfahren und system zur genomanalyse
JP6295578B2 (ja) * 2013-09-30 2018-03-20 凸版印刷株式会社 反応容器、核酸解析装置、および核酸解析方法
GB2535066A (en) 2013-10-03 2016-08-10 Personalis Inc Methods for analyzing genotypes
EP3087204B1 (de) 2013-12-28 2018-02-14 Guardant Health, Inc. Verfahren und systeme für den nachweis genetischer varianten
PT4026917T (pt) * 2014-04-14 2024-02-12 Yissum Research And Development Company Of The Hebrew Univ Of Jerusalem Ltd Método e kit para determinar a morte de células ou de tecido ou a origem de tecidos ou de células de dna por análise de metilação do dna
EP3169813B1 (de) 2014-07-18 2019-06-12 The Chinese University Of Hong Kong Methylierungsmusteranalyse von gewebe in einem dna-gemisch
EP4026913A1 (de) 2014-10-30 2022-07-13 Personalis, Inc. Verfahren zur verwendung von mosaizismus in nukleinsäuren, die distal zu ihrem ursprung entnommen wurden
US11168351B2 (en) 2015-03-05 2021-11-09 Streck, Inc. Stabilization of nucleic acids in urine
US20170145475A1 (en) 2015-11-20 2017-05-25 Streck, Inc. Single spin process for blood plasma separation and plasma composition including preservative
AU2016370835B2 (en) * 2015-12-17 2020-02-13 Illumina, Inc. Distinguishing methylation levels in complex biological samples
EP3390668A4 (de) 2015-12-17 2020-04-01 Guardant Health, Inc. Verfahren zur bestimmung der tumorgenkopienzahl durch analyse von zellfreier dna
US11299783B2 (en) 2016-05-27 2022-04-12 Personalis, Inc. Methods and systems for genetic analysis
US10781490B2 (en) 2016-05-30 2020-09-22 The Chinese University Of Hong Kong Detecting hematological disorders using cell-free DNA in blood
CN116445593A (zh) 2016-08-10 2023-07-18 格里尔公司 测定一生物样品的一甲基化图谱的方法
US9850523B1 (en) 2016-09-30 2017-12-26 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
AU2017336153B2 (en) 2016-09-30 2023-07-13 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
EP3535415A4 (de) 2016-10-24 2020-07-01 The Chinese University of Hong Kong Verfahren und systeme zur tumordetektion
IL315032A (en) 2016-11-30 2024-10-01 The Chinese Univ Of Hong Kong Analysis of cell-free DNA in urine and other samples
TWI803477B (zh) 2017-01-25 2023-06-01 香港中文大學 使用核酸片段之診斷應用
EP3366780B1 (de) 2017-02-23 2020-05-06 Siemens Healthcare GmbH Sequenzanalyse von einzelmolekülen und hochempfindliche methylierungsanalyse zur gewebespezifischen analyse
US11179516B2 (en) 2017-06-22 2021-11-23 Baxter International Inc. Systems and methods for incorporating patient pressure into medical fluid delivery
AU2019234843A1 (en) 2018-03-13 2020-09-24 Grail, Llc Anomalous fragment detection and classification
WO2019195268A2 (en) 2018-04-02 2019-10-10 Grail, Inc. Methylation markers and targeted methylation probe panels
US10801064B2 (en) 2018-05-31 2020-10-13 Personalis, Inc. Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples
US11814750B2 (en) 2018-05-31 2023-11-14 Personalis, Inc. Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples
WO2020069350A1 (en) 2018-09-27 2020-04-02 Grail, Inc. Methylation markers and targeted methylation probe panel
CN113661249A (zh) 2019-01-31 2021-11-16 夸登特健康公司 用于分离无细胞dna的组合物和方法
US11427874B1 (en) 2019-08-26 2022-08-30 Epi One Inc. Methods and systems for detection of prostate cancer by DNA methylation analysis
WO2021154893A1 (en) 2020-01-30 2021-08-05 Prognomiq Inc Lung biomarkers and methods of use thereof
EP4347886A1 (de) 2021-06-03 2024-04-10 Inherent Biosciences, Inc. Dna-methylierungsanalyse zur identifizierung des zelltyps
EP4402473A1 (de) 2021-09-13 2024-07-24 Prognomiq Inc Verbesserter nachweis und quantifizierung von biomolekülen
CN116042848B (zh) * 2021-10-28 2024-08-09 北京干细胞与再生医学研究院 一种通过血样检测判断动物体内存在移植物的方法

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4897355A (en) * 1985-01-07 1990-01-30 Syntex (U.S.A.) Inc. N[ω,(ω-1)-dialkyloxy]- and N-[ω,(ω-1)-dialkenyloxy]-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
EP0655090B1 (de) * 1992-04-27 2000-12-27 The Trustees Of Dartmouth College Detektion von gensequenzen in biologischen flüssigkeiten
US6020124A (en) * 1992-04-27 2000-02-01 Trustees Of Dartmouth College Detection of soluble gene sequences in biological fluids
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
CH686982A5 (fr) * 1993-12-16 1996-08-15 Maurice Stroun Méthode pour le diagnostic de cancers.
US5552277A (en) * 1994-07-19 1996-09-03 The Johns Hopkins University School Of Medicine Genetic diagnosis of prostate cancer
DE4441057C2 (de) * 1994-11-17 1999-02-25 Wacker Chemie Gmbh Verfahren zur kontinuierlichen Herstellung von Organopolysiloxanen
WO1996034983A1 (en) * 1995-05-05 1996-11-07 The Perkin-Elmer Corporation Methods and reagents for combined pcr amplification and hybridization probing assay
CA2248981C (en) * 1996-03-15 2009-11-24 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays
US6017704A (en) * 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
EP0956365A4 (de) * 1996-08-28 2004-08-25 Univ Johns Hopkins Med Verfahren zur detektion zellproliferativer deregulationen
US6251594B1 (en) * 1997-06-09 2001-06-26 Usc/Norris Comprehensive Cancer Ctr. Cancer diagnostic method based upon DNA methylation differences
DE19754482A1 (de) * 1997-11-27 1999-07-01 Epigenomics Gmbh Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
EP1052293B1 (de) * 1999-05-12 2003-12-17 Hamamatsu Photonics K.K. Nachweis von Nukleinsäuren im Zytoplasma
US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
ATE318891T1 (de) * 2000-05-22 2006-03-15 Univ Johns Hopkins Testverfahren zur bestimmung von gen-imprinting und methylierte cpg inseln
US6927028B2 (en) * 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
US7045319B2 (en) * 2001-10-30 2006-05-16 Ribomed Biotechnologies, Inc. Molecular detection systems utilizing reiterative oligonucleotide synthesis

Also Published As

Publication number Publication date
ES2253461T3 (es) 2006-06-01
DK1342794T3 (da) 2006-04-24
DE60207979D1 (de) 2006-01-19
EP1483410A1 (de) 2004-12-08
DE60207979T2 (de) 2006-09-28
US20050221314A1 (en) 2005-10-06
WO2003074730A1 (en) 2003-09-12
AU2003218691B2 (en) 2008-06-12
CA2478493A1 (en) 2003-09-12
JP4727149B2 (ja) 2011-07-20
EP1342794B1 (de) 2005-12-14
EP1342794A1 (de) 2003-09-10
AU2003218691A1 (en) 2003-09-16
JP2005518809A (ja) 2005-06-30

Similar Documents

Publication Publication Date Title
ATE312946T1 (de) Verfahren und vorrichtung zur bestimmung der gewebespezifität von freier dna in körperflüssigkeiten
RU2008103988A (ru) Способ диагностики рассеяного склероза
NO20030251L (no) Fremgangsmåte for differensiering mellom biologiske tilstander basert på skjulte mönstre fra biologiske data
ATE447715T1 (de) Automatische glykofingerabdruck-strategie
EP2306192A3 (de) Risikomarker für eine Herzkreislaufkrankheit
MX2007014815A (es) Metodos de inmunoanalisis mejorados.
EE200300005A (et) Meetod konformse haiguse diagnoosimiseks või tuvastamiseks, selleks vajalik katsemenetlus, reaktiivikomplekt ning seade
KR20150084802A (ko) 진단 장치 및 방법
JP2010539490A5 (de)
ATE365922T1 (de) Verfahren zur erkennung von eierstockkrebs mittels menschlichem kallikrein (hk6)
ATE423217T1 (de) Assays zur überwachung von krebspatienten auf grundlage der spiegel von analytenkomponenten des plasminogen-aktivator-systems in proben von körperflüssigkeiten
EP1480251A3 (de) Analyse komplexer Mischungen von biologischen und anderen Flüssigkeiten zur Erfassung ihrer Biologischen Eigenschaften.
CA2623653A1 (en) Use of n-myristoyltransferase on non-tumor tissue for cancer diagnosis
WO2002098280A3 (en) Method for detecting infectious agents using computer controlled automated image analysis
WO2005081783A3 (en) Method for determination and quantification of radiation or genotoxin exposure
WO2021038112A3 (en) Apparatus and method for determination of banned substances
EP1159614A4 (de) Diagnostisches verfahren für asthma
ATE408145T1 (de) Verfahren zum nachweis von nierenkrankheiten und zur pathologischen überwachung
DE69928245D1 (de) Verfahren zur probenentnahme
KR20200049647A (ko) 반려동물에서 종양질병의 보조적 진단을 위한 바이오마커 검사방법
EP1376133A4 (de) Screening-verfahren für den prädiabetiszustand und screening-reagenz
WO2020176569A1 (en) Device for methods of detecting cancer
ATE352037T1 (de) Immunochromatographiesystem und verfahren zur gleichzeitigen identifikation von aga-und t-tg- antikörpern und verwendung dafür zur diagnose der zöliakiekrankheit
ATE261120T1 (de) Anhand der konduktivität normalisierte bestimmung von analyten in urin zur diagnose von krankheiten
ATE442456T1 (de) Verfahren zur bestimmung von zirkulierender dns in plasma und zum nachweis von krebs

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1342794

Country of ref document: EP