ATE303403T1 - Modifizierte rekombinase - Google Patents

Modifizierte rekombinase

Info

Publication number
ATE303403T1
ATE303403T1 AT01993622T AT01993622T ATE303403T1 AT E303403 T1 ATE303403 T1 AT E303403T1 AT 01993622 T AT01993622 T AT 01993622T AT 01993622 T AT01993622 T AT 01993622T AT E303403 T1 ATE303403 T1 AT E303403T1
Authority
AT
Austria
Prior art keywords
recombinase
fusion protein
genome
cell
site
Prior art date
Application number
AT01993622T
Other languages
English (en)
Inventor
Ralf Kuehn
Susanne Felder
Frieder Schwenk
Birgit Kueter-Luks
Nicole Faust
Original Assignee
Artemis Pharmaceuticals Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP00124629A external-priority patent/EP1205490A1/de
Application filed by Artemis Pharmaceuticals Gmbh filed Critical Artemis Pharmaceuticals Gmbh
Application granted granted Critical
Publication of ATE303403T1 publication Critical patent/ATE303403T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/22011—Polyomaviridae, e.g. polyoma, SV40, JC
    • C12N2710/22022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
AT01993622T 2000-11-10 2001-11-09 Modifizierte rekombinase ATE303403T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP00124629A EP1205490A1 (de) 2000-11-10 2000-11-10 Fusionsprotein, das eine Integrase (phiC31) und ein Signalpeptid (NLS) enthält
EP01109543 2001-04-17
US31187601P 2001-08-13 2001-08-13
PCT/EP2001/012975 WO2002038613A2 (en) 2000-11-10 2001-11-09 Modified recombinase

Publications (1)

Publication Number Publication Date
ATE303403T1 true ATE303403T1 (de) 2005-09-15

Family

ID=56290218

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01993622T ATE303403T1 (de) 2000-11-10 2001-11-09 Modifizierte rekombinase

Country Status (5)

Country Link
EP (1) EP1395612B1 (de)
AT (1) ATE303403T1 (de)
AU (1) AU2002221829A1 (de)
DE (1) DE60113159T2 (de)
WO (1) WO2002038613A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1255868B1 (de) 2000-02-18 2010-12-01 The Board Of Trustees Of The Leland Stanford Junior University Veränderte rekombinasen zur modifizierung des genoms
WO2003066867A2 (en) * 2002-02-06 2003-08-14 Artemis Pharmaceuticals Gmbh Genetically engineered phic31-integrase genes
DE10207313A1 (de) * 2002-02-21 2003-09-11 Vision 7 Gmbh Verfahren zur ortsspezifischen DNA-Rekombination
EP1439234A1 (de) * 2003-01-08 2004-07-21 ARTEMIS Pharmaceuticals GmbH Gezielte Transgenese unter Verwendung des rosa26-Lokus
EP1483962A3 (de) * 2003-06-06 2005-01-12 F. Hoffmann-La Roche Ag Transgenes Tiermodel für Glyt1 Funktion
US9034650B2 (en) * 2005-02-02 2015-05-19 Intrexon Corporation Site-specific serine recombinases and methods of their use
CN101511994A (zh) * 2006-05-22 2009-08-19 海普罗塞尔有限公司 使用真核细胞系生成蛋白质
CN100410369C (zh) * 2006-06-22 2008-08-13 复旦大学 经tat和nls多肽修饰的φc31整合酶蛋白及其应用
JP2010514416A (ja) * 2006-12-21 2010-05-06 グラクソスミスクライン・リミテッド・ライアビリティ・カンパニー 新規な方法

Also Published As

Publication number Publication date
AU2002221829A1 (en) 2002-05-21
WO2002038613A2 (en) 2002-05-16
EP1395612B1 (de) 2005-08-31
EP1395612A2 (de) 2004-03-10
DE60113159T2 (de) 2006-07-06
DE60113159D1 (de) 2005-10-06
WO2002038613A3 (en) 2004-01-08

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