DE60143564D1 - Veränderte rekombinasen zur modifizierung des genoms - Google Patents

Veränderte rekombinasen zur modifizierung des genoms

Info

Publication number
DE60143564D1
DE60143564D1 DE60143564T DE60143564T DE60143564D1 DE 60143564 D1 DE60143564 D1 DE 60143564D1 DE 60143564 T DE60143564 T DE 60143564T DE 60143564 T DE60143564 T DE 60143564T DE 60143564 D1 DE60143564 D1 DE 60143564D1
Authority
DE
Germany
Prior art keywords
wild
recombinase
type
altered
parent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60143564T
Other languages
English (en)
Inventor
Michele P Calos
Christopher R Sclimenti
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
R UNIVERSITY
Original Assignee
R UNIVERSITY
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22674167&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60143564(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by R UNIVERSITY filed Critical R UNIVERSITY
Application granted granted Critical
Publication of DE60143564D1 publication Critical patent/DE60143564D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60143564T 2000-02-18 2001-02-16 Veränderte rekombinasen zur modifizierung des genoms Expired - Lifetime DE60143564D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18375900P 2000-02-18 2000-02-18
PCT/US2001/005269 WO2001061049A1 (en) 2000-02-18 2001-02-16 Altered recombinases for genome modification

Publications (1)

Publication Number Publication Date
DE60143564D1 true DE60143564D1 (de) 2011-01-13

Family

ID=22674167

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60143564T Expired - Lifetime DE60143564D1 (de) 2000-02-18 2001-02-16 Veränderte rekombinasen zur modifizierung des genoms

Country Status (9)

Country Link
US (5) US6808925B2 (de)
EP (1) EP1255868B1 (de)
JP (1) JP5033288B2 (de)
AT (1) ATE490338T1 (de)
AU (2) AU3979201A (de)
CA (1) CA2400087A1 (de)
DE (1) DE60143564D1 (de)
ES (1) ES2355493T3 (de)
WO (1) WO2001061049A1 (de)

Families Citing this family (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6746870B1 (en) * 1999-07-23 2004-06-08 The Regents Of The University Of California DNA recombination in eukaryotic cells by the bacteriophage PHIC31 recombination system
US20090263851A1 (en) * 2001-03-19 2009-10-22 Liu David R In vivo selection system for enzyme activity
US7476500B1 (en) * 2001-03-19 2009-01-13 President And Fellows Of Harvard College In vivo selection system for enzyme activity
US7294507B2 (en) * 2001-11-30 2007-11-13 Avigenics, Inc. Ovomucoid promoters and methods of use
US7335761B2 (en) * 2001-11-30 2008-02-26 Avigenics, Inc. Avian gene expression controlling regions
US20100333219A1 (en) * 2001-11-30 2010-12-30 Synageva Biopharma Corp. Methods of protein production using ovomucoid regulatory regions
WO2003066867A2 (en) * 2002-02-06 2003-08-14 Artemis Pharmaceuticals Gmbh Genetically engineered phic31-integrase genes
WO2005017170A2 (en) 2002-06-04 2005-02-24 Michele Calos Methods of unidirectional, site-specific integration into a genome, compositions and kits for practicing the same
US8304233B2 (en) 2002-06-04 2012-11-06 Poetic Genetics, Llc Methods of unidirectional, site-specific integration into a genome, compositions and kits for practicing the same
US20040255345A1 (en) * 2003-03-07 2004-12-16 Rapp Jeffrey C. Production of transgenic avians
US20050034186A1 (en) * 2003-03-07 2005-02-10 Harvey Alex J. Site specific nucleic acid integration
EP1608219A4 (de) * 2003-03-07 2007-03-14 Avigenics Inc Integrase-vermittelte vogeltransgenese
US20050273873A1 (en) * 2003-03-07 2005-12-08 Avigenics, Inc. Genomic modification
US20050198700A1 (en) * 2003-03-07 2005-09-08 Avigenics, Inc. Genomic modification
US7381712B2 (en) * 2003-05-09 2008-06-03 Avigenics, Inc. In vivo transfection in avians
US20080222744A1 (en) * 2003-05-09 2008-09-11 Avigenics, Inc. In vivo transfection in avians
EP1644538A4 (de) * 2003-06-26 2006-11-08 Invitrogen Corp Verfahren und zusammensetzungen zum nachweis von promotoraktivität und zur expression von fusionsproteinen
EP1708564A4 (de) * 2004-01-15 2007-03-07 Avigenics Inc Produktion eines transgenen vogels mittels cytoplasmainjektion
US20060123504A1 (en) * 2004-12-07 2006-06-08 Avigenics, Inc. Methods of producing polyclonal antibodies
US20060174364A1 (en) * 2004-03-01 2006-08-03 Avigenics, Inc. Artificial chromosomes and transchromosomic avians
JP4769796B2 (ja) * 2004-04-30 2011-09-07 ポエティック ジェネティックス エルエルシー ゲノム操作のためのハイブリッド型リコンビナーゼ
US20070190601A1 (en) * 2004-10-08 2007-08-16 Avigenics, Inc. Modified integrase and methods of use
NZ588430A (en) 2004-12-22 2012-10-26 Genentech Inc Methods for producing soluble multi-membrane-spanning proteins
US9034650B2 (en) * 2005-02-02 2015-05-19 Intrexon Corporation Site-specific serine recombinases and methods of their use
US8101169B2 (en) * 2005-02-23 2012-01-24 The Board Of Trustees Of The Leland Stanford Junior University Ocular gene therapy using avalanche-mediated transfection
US7923251B2 (en) * 2005-02-23 2011-04-12 The Board Of Trustees Of The Leland Stanford Junior University Method and apparatus for avalanche-mediated transfer of agents into cells
US20070031390A1 (en) * 2005-05-18 2007-02-08 Daniel Weeks Methods and compositions for site-specific genomic expression of nucleic acid sequences
US9274099B2 (en) 2005-07-22 2016-03-01 The Board Of Trustees Of The Leland Stanford Junior University Screening test drugs to identify their effects on cell membrane voltage-gated ion channel
US9278159B2 (en) 2005-07-22 2016-03-08 The Board Of Trustees Of The Leland Stanford Junior University Light-activated cation channel and uses thereof
US8926959B2 (en) 2005-07-22 2015-01-06 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
US9238150B2 (en) 2005-07-22 2016-01-19 The Board Of Trustees Of The Leland Stanford Junior University Optical tissue interface method and apparatus for stimulating cells
US10052497B2 (en) 2005-07-22 2018-08-21 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
US20090093403A1 (en) 2007-03-01 2009-04-09 Feng Zhang Systems, methods and compositions for optical stimulation of target cells
EP2034954B1 (de) * 2006-03-30 2019-02-20 Engene, Inc. Nicht virale zusammensetzungen und verfahren zur in-vivo-transfektion von darmzellen
WO2007137114A2 (en) 2006-05-17 2007-11-29 Pioneer Hi-Bred International, Inc. Artificial plant minichromosomes
BRPI0711207A2 (pt) 2006-05-22 2011-03-22 Hiprocell Llc vetor alvo para intregação em sìtios-especificos, método de sìtio-especìfico que integra um polinucleotìdeo codificando uma proteina de interesse em um genoma de uma célula eucariótia, célula eucariótica isolada , kit para uso no sìtio-especìfico que integra um polinucleotideo dentro de um genoma de uma celula in vitro e kit para uso em produzir uma proteìna em célula
WO2008086470A1 (en) 2007-01-10 2008-07-17 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
WO2008101128A1 (en) * 2007-02-14 2008-08-21 The Board Of Trustees Of The Leland Stanford Junior University System, method and applications involving identification of biological circuits such as neurological characteristics
US10434327B2 (en) 2007-10-31 2019-10-08 The Board Of Trustees Of The Leland Stanford Junior University Implantable optical stimulators
US10035027B2 (en) * 2007-10-31 2018-07-31 The Board Of Trustees Of The Leland Stanford Junior University Device and method for ultrasonic neuromodulation via stereotactic frame based technique
JP5801188B2 (ja) 2008-04-23 2015-10-28 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 標的細胞を光刺激するためのシステム、方法、および組成物
MX2010012986A (es) * 2008-05-29 2011-05-25 Univ Leland Stanford Junior Linea de celulas, sistema y metodo para control optico de mensajeros secundarios.
MY162929A (en) * 2008-06-17 2017-07-31 Univ Leland Stanford Junior Apparatus and methods for controlling cellular development
SG191593A1 (en) 2008-06-17 2013-07-31 Univ Leland Stanford Junior Methods, systems and devices for optical stimulation of target cells using an optical transmission element
WO2010006049A1 (en) 2008-07-08 2010-01-14 The Board Of Trustees Of The Leland Stanford Junior University Materials and approaches for optical stimulation of the peripheral nervous system
WO2010019619A1 (en) * 2008-08-11 2010-02-18 The Board Of Trustees Of The Leland Stanford Junior Univeristy Method and composition for controlling gene expression
CA3059768A1 (en) 2008-09-05 2010-03-11 President And Fellows Of Harvard College Continuous directed evolution of proteins and nucleic acids
NZ602416A (en) 2008-11-14 2014-08-29 Univ Leland Stanford Junior Optically-based stimulation of target cells and modifications thereto
DK2462230T3 (en) * 2009-08-03 2015-10-19 Recombinetics Inc METHODS AND COMPOSITIONS FOR TARGETED RE-MODIFICATION
JP5866332B2 (ja) 2010-03-17 2016-02-17 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 感光性イオンを通過させる分子
CA2816971A1 (en) 2010-11-05 2012-05-10 The Board Of Trustees Of The Leland Stanford Junior University Light-activated chimeric opsins and methods of using the same
WO2012061684A1 (en) 2010-11-05 2012-05-10 The Board Of Trustees Of The Leland Stanford Junior University Upconversion of light for use in optogenetic methods
AU2011323228B2 (en) 2010-11-05 2016-11-10 The Board Of Trustees Of The Leland Stanford Junior University Control and characterization of memory function
CN106106368A (zh) 2010-11-05 2016-11-16 斯坦福大学托管董事会 光控cns功能障碍
CA2816990A1 (en) 2010-11-05 2012-05-10 The Board Of Trustees Of The Leland Stanford Junior University Stabilized step function opsin proteins and methods of using the same
EP2635346B1 (de) 2010-11-05 2017-03-29 The Board of Trustees of the Leland Stanford Junior University Optogenetische steuerung eines verhaltens in zusammenhang mit belohnungen
WO2012064970A1 (en) 2010-11-12 2012-05-18 The Board Of Trustees Of The Leland Stanford Junior University Site-directed integration of transgenes in mammals
US8696722B2 (en) 2010-11-22 2014-04-15 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic magnetic resonance imaging
AU2011348204B2 (en) 2010-12-22 2017-03-02 President And Fellows Of Harvard College Continuous directed evolution
CA2828239C (en) 2011-02-25 2020-10-06 Recombinetics, Inc. Genetically modified animals and methods for making the same
US9528124B2 (en) 2013-08-27 2016-12-27 Recombinetics, Inc. Efficient non-meiotic allele introgression
US10920242B2 (en) 2011-02-25 2021-02-16 Recombinetics, Inc. Non-meiotic allele introgression
EP2739738B1 (de) 2011-08-03 2018-06-20 Ramot at Tel Aviv University, Ltd. Verwendung von integrase für gezielte genexpression
WO2013090356A2 (en) 2011-12-16 2013-06-20 The Board Of Trustees Of The Leland Stanford Junior University Opsin polypeptides and methods of use thereof
CA2865296A1 (en) 2012-02-21 2013-08-29 Karl A. DEISSEROTH Compositions and methods for treating neurogenic disorders of the pelvic floor
AR091482A1 (es) 2012-06-21 2015-02-04 Recombinetics Inc Celulas modificadas geneticamente y metodos par su obtencion
US9636380B2 (en) 2013-03-15 2017-05-02 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic control of inputs to the ventral tegmental area
EP2968997B1 (de) 2013-03-15 2019-06-26 The Board of Trustees of the Leland Stanford Junior University Optogenetische steuerung des verhaltenszustandes
CN105431046B (zh) 2013-04-29 2020-04-17 小利兰·斯坦福大学托管委员会 用于靶细胞中的动作电位的光遗传学调节的装置、系统和方法
WO2015023782A1 (en) 2013-08-14 2015-02-19 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for controlling pain
US10179911B2 (en) 2014-01-20 2019-01-15 President And Fellows Of Harvard College Negative selection and stringency modulation in continuous evolution systems
US10920208B2 (en) 2014-10-22 2021-02-16 President And Fellows Of Harvard College Evolution of proteases
WO2016168631A1 (en) 2015-04-17 2016-10-20 President And Fellows Of Harvard College Vector-based mutagenesis system
WO2016209654A1 (en) 2015-06-22 2016-12-29 The Board Of Trustees Of The Leland Stanford Junior University Methods and devices for imaging and/or optogenetic control of light-responsive neurons
US10392674B2 (en) 2015-07-22 2019-08-27 President And Fellows Of Harvard College Evolution of site-specific recombinases
US11524983B2 (en) 2015-07-23 2022-12-13 President And Fellows Of Harvard College Evolution of Bt toxins
WO2017019895A1 (en) 2015-07-30 2017-02-02 President And Fellows Of Harvard College Evolution of talens
FR3041655B1 (fr) * 2015-09-29 2017-11-24 Lesaffre & Cie Nouvelles souches bacteriennes pour la production de vanilline
JP2018038333A (ja) * 2016-09-08 2018-03-15 ライフテクノロジーズジャパン株式会社 細胞の作製方法、発現用ベクター及び細胞
US11294165B2 (en) 2017-03-30 2022-04-05 The Board Of Trustees Of The Leland Stanford Junior University Modular, electro-optical device for increasing the imaging field of view using time-sequential capture
US11447809B2 (en) 2017-07-06 2022-09-20 President And Fellows Of Harvard College Evolution of tRNA synthetases
WO2019056002A1 (en) 2017-09-18 2019-03-21 President And Fellows Of Harvard College CONTINUOUS EVOLUTION FOR STABILIZED PROTEINS
EP3749754A4 (de) * 2018-02-08 2021-10-20 Applied Stemcell, Inc. Verfahren für das screening von varianten eines zielgens
US11913044B2 (en) 2018-06-14 2024-02-27 President And Fellows Of Harvard College Evolution of cytidine deaminases
GB202006462D0 (en) * 2020-05-04 2020-06-17 Mote Res Limited Modifying genomes with integrase

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5817491A (en) * 1990-09-21 1998-10-06 The Regents Of The University Of California VSV G pseusdotyped retroviral vectors
CA2226463A1 (en) * 1995-06-07 1996-12-19 Life Technologies, Inc. Recombinational cloning using engineered recombination sites
US6890726B1 (en) * 1999-04-06 2005-05-10 Oklahoma Medical Research Foundation Method for selecting recombinase variants with altered specificity
AU4205500A (en) * 1999-04-06 2000-10-23 Oklahoma Medical Research Foundation Method for selecting recombinase variants with altered specificity
WO2002038613A2 (en) * 2000-11-10 2002-05-16 Artemis Pharmaceuticals Gmbh Modified recombinase

Also Published As

Publication number Publication date
WO2001061049A1 (en) 2001-08-23
EP1255868B1 (de) 2010-12-01
US20050003540A1 (en) 2005-01-06
AU2001239792B2 (en) 2006-05-18
JP2003528594A (ja) 2003-09-30
CA2400087A1 (en) 2001-08-23
EP1255868A1 (de) 2002-11-13
US7732585B2 (en) 2010-06-08
AU3979201A (en) 2001-08-27
US20080167261A1 (en) 2008-07-10
US7141426B2 (en) 2006-11-28
US20100190178A1 (en) 2010-07-29
EP1255868A4 (de) 2004-12-01
ATE490338T1 (de) 2010-12-15
US6808925B2 (en) 2004-10-26
JP5033288B2 (ja) 2012-09-26
ES2355493T3 (es) 2011-03-28
US20020094516A1 (en) 2002-07-18
US20070077589A1 (en) 2007-04-05

Similar Documents

Publication Publication Date Title
DE60143564D1 (de) Veränderte rekombinasen zur modifizierung des genoms
ATE317440T1 (de) Neuartige methode zur integration von fremd-dna ins eukaryotische genom
ES2689353T3 (es) Método para construir una biblioteca de ácidos nucleicos cíclicos de cadena sencilla y reactivos de los mismos
DE60208014D1 (de) Verfahren zur herstellung einer stabilen inversion einer dna-sequenz durch ortsspezifische rekombination und dna-vektoren und transgene zellen davon
TR200800453T1 (tr) Yüksek düzeyde lisin 'li mısır kompozisyonları ve bunların deteksiyonuna yönelik metotlar.
ATE509110T1 (de) Canola event pv-bngt(rt73), zusammensetzungen und verfahren zum nachweis davon
ATE384126T1 (de) Interferon induzierende genetisch veränderte attenuierte viren
CY1106109T1 (el) Συνθεσεις και μεθοδοι για ανιχνευση μετασχηματιστικου παραγοντος pvzmgt32 (nk 603) αραβοσιτου
DK0973928T3 (da) DNA-baseret transposonsystem til indføring af nukleinsyre i DNA i en celle
MY140680A (en) Hepatitis c virus inhibitors
WO2002014495A3 (en) Enhanced homologous recombination mediated by lambda recombination proteins
WO2005054430A3 (en) Hepatitis c virus inhibitors
ATE437233T1 (de) Matrix-anheftungsregionen und verfahren zu deren verwendung
DK0937098T3 (da) Rekombinatorisk kloning under anvendelse af gensplejsede rekombinationssites
WO2005003315A3 (en) Compositions and methods for peptide-assisted transfection
EA200500448A1 (ru) Способ культивирования первичных клеток и амплификации вирусов в бессывороточных условиях
WO2002024917A3 (de) Verfahren zur veränderung des genoms von corynebakterien
TR200001046T2 (tr) Homolog rekombinasyonda pozitif-negatif seçimi.
EP1689886A4 (de) Mit cmv-resistenz assoziierter molekularer marker und verwendung davon
ATE408013T1 (de) Infektiöses cdna klon des gb-virus b und dessen verwendungen
DE60113159D1 (de) Modifizierte rekombinase
WO2002020737A3 (en) Genome engineering by cell-permeable dna site-specific recombinases
WO2001092558A3 (en) End sequence profiling
EP1728869A3 (de) Gen-Expressionskonstrukt von Hap1 modifiziert für Planzenzellen
Acquadro et al. RAD2seq: an efficient protocol for plant genotyping by sequencing