DE60113159D1 - Modifizierte rekombinase - Google Patents

Modifizierte rekombinase

Info

Publication number
DE60113159D1
DE60113159D1 DE60113159T DE60113159T DE60113159D1 DE 60113159 D1 DE60113159 D1 DE 60113159D1 DE 60113159 T DE60113159 T DE 60113159T DE 60113159 T DE60113159 T DE 60113159T DE 60113159 D1 DE60113159 D1 DE 60113159D1
Authority
DE
Germany
Prior art keywords
recombinase
fusion protein
genome
cell
site
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60113159T
Other languages
English (en)
Other versions
DE60113159T2 (de
Inventor
Ralf Kuehn
Susanne Felder
Frieder Schwenk
Birgit Kueter-Luks
Nicole Faust
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Artemis Pharmaceuticals GmbH
Original Assignee
Artemis Pharmaceuticals GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP00124629A external-priority patent/EP1205490A1/de
Application filed by Artemis Pharmaceuticals GmbH filed Critical Artemis Pharmaceuticals GmbH
Application granted granted Critical
Publication of DE60113159D1 publication Critical patent/DE60113159D1/de
Publication of DE60113159T2 publication Critical patent/DE60113159T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/22011—Polyomaviridae, e.g. polyoma, SV40, JC
    • C12N2710/22022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Dairy Products (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Emulsifying, Dispersing, Foam-Producing Or Wetting Agents (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Saccharide Compounds (AREA)
DE60113159T 2000-11-10 2001-11-09 Modifizierte rekombinase Expired - Fee Related DE60113159T2 (de)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
EP00124629 2000-11-10
EP00124629A EP1205490A1 (de) 2000-11-10 2000-11-10 Fusionsprotein, das eine Integrase (phiC31) und ein Signalpeptid (NLS) enthält
EP01109543 2001-04-17
EP01109543 2001-04-17
US31187601P 2001-08-13 2001-08-13
US311876P 2001-08-13
PCT/EP2001/012975 WO2002038613A2 (en) 2000-11-10 2001-11-09 Modified recombinase

Publications (2)

Publication Number Publication Date
DE60113159D1 true DE60113159D1 (de) 2005-10-06
DE60113159T2 DE60113159T2 (de) 2006-07-06

Family

ID=56290218

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60113159T Expired - Fee Related DE60113159T2 (de) 2000-11-10 2001-11-09 Modifizierte rekombinase

Country Status (5)

Country Link
EP (1) EP1395612B1 (de)
AT (1) ATE303403T1 (de)
AU (1) AU2002221829A1 (de)
DE (1) DE60113159T2 (de)
WO (1) WO2002038613A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001239792B2 (en) 2000-02-18 2006-05-18 The Board Of Trustees Of The Leland Stanford Junior University Altered recombinases for genome modification
AU2003206841A1 (en) * 2002-02-06 2003-09-02 Artemis Pharmaceuticals Gmbh Genetically engineered phic31-integrase genes
DE10207313A1 (de) * 2002-02-21 2003-09-11 Vision 7 Gmbh Verfahren zur ortsspezifischen DNA-Rekombination
EP1439234A1 (de) * 2003-01-08 2004-07-21 ARTEMIS Pharmaceuticals GmbH Gezielte Transgenese unter Verwendung des rosa26-Lokus
EP1483962A3 (de) * 2003-06-06 2005-01-12 F. Hoffmann-La Roche Ag Transgenes Tiermodel für Glyt1 Funktion
US9034650B2 (en) * 2005-02-02 2015-05-19 Intrexon Corporation Site-specific serine recombinases and methods of their use
AU2007254508B2 (en) * 2006-05-22 2012-03-22 Hiprocell, Llc Protein production using eukaryotic cell lines
CN100410369C (zh) * 2006-06-22 2008-08-13 复旦大学 经tat和nls多肽修饰的φc31整合酶蛋白及其应用
US20100105042A1 (en) * 2006-12-21 2010-04-29 Smithkline Beecham Corporation Novel methods

Also Published As

Publication number Publication date
ATE303403T1 (de) 2005-09-15
EP1395612A2 (de) 2004-03-10
EP1395612B1 (de) 2005-08-31
DE60113159T2 (de) 2006-07-06
WO2002038613A3 (en) 2004-01-08
AU2002221829A1 (en) 2002-05-21
WO2002038613A2 (en) 2002-05-16

Similar Documents

Publication Publication Date Title
RU2004107694A (ru) Способ интеграции генов в специфических сайтах в клетках млекопитающих посредством гомологичной рекомбинации и векторы для осуществления этого способа
DE60143564D1 (de) Veränderte rekombinasen zur modifizierung des genoms
CN102787125B (zh) 一种构建tale重复序列的方法
DE69427997D1 (de) Hybrider mensch/tier faktor viii
US8796183B2 (en) Template fixed beta-hairpin loop mimetics and their use in phage display
ATE303403T1 (de) Modifizierte rekombinase
EA199900503A1 (ru) Днк, кодирующая активные дельта-эндотоксины чешуекрылых насекомых, и ее использование
ES2167304T3 (es) Peptido que contiene el antigeno de superficie de la hepatitis b.
ATE317440T1 (de) Neuartige methode zur integration von fremd-dna ins eukaryotische genom
DE60035871D1 (de) Expression und export von interferon-alpha proteinen als fc fusionsproteine
ATE469231T1 (de) Von rna-virus abgeleitetes pflanzenexpressionssystem
PT937098E (pt) Clonagem recombinatoria in vitro utilizando locais de recombinacao modificados
ATE423213T1 (de) Erhöhte expression in pflanzen durch verwendung von nicht-translierter leader-sequenz
ATE443074T1 (de) Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
EA199800324A1 (ru) Улучшенное встраивание экзогенной днк, введенной в эукариостические клетки
DE60144021D1 (de) Für zellzyklusproteine kodierende nukleinsäure und deren verwendung
Osawa et al. Evolution of life: fossils, molecules and culture
ES2378561T3 (es) Biblioteca de oligonucleótidos que codifican péptidos aleatorizados
Piepers et al. GreenGate 2.0: Backwards compatible addons for assembly of complex transcriptional units and their stacking with GreenGate
DK0915165T3 (da) Fremgangsmåde til hjælper-virusfri pakning af et genvektor-DNA
Hamer Recombinant DNA—an introduction
RU2022100100A (ru) Конструкции для направленного редактирования генов и способы с их применением
Rentas Molecular genetic and biochemical analysis of a putative terminase cutting site in the large DNA packaging protein gp17 from bacteriophage T4
Mustafa Targeted Genome Editing using Single Chain Zinc Finger Dimeric Nuclease
Soeding et al. On the Evolution of Proteins from Peptides

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee