ATE297980T1 - Verfahren zur klonierung von schweinen - Google Patents

Verfahren zur klonierung von schweinen

Info

Publication number
ATE297980T1
ATE297980T1 AT99958900T AT99958900T ATE297980T1 AT E297980 T1 ATE297980 T1 AT E297980T1 AT 99958900 T AT99958900 T AT 99958900T AT 99958900 T AT99958900 T AT 99958900T AT E297980 T1 ATE297980 T1 AT E297980T1
Authority
AT
Austria
Prior art keywords
cloning
cells
porcine
relates
porcine animals
Prior art date
Application number
AT99958900T
Other languages
English (en)
Inventor
Philip Damiani
Jeffrey M Betthauser
Erik J Forsberg
Michael D Bishop
Original Assignee
Infigen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Infigen Inc filed Critical Infigen Inc
Application granted granted Critical
Publication of ATE297980T1 publication Critical patent/ATE297980T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8778—Swine embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0611—Primordial germ cells, e.g. embryonic germ cells [EG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)
  • Peptides Or Proteins (AREA)
AT99958900T 1998-11-24 1999-11-12 Verfahren zur klonierung von schweinen ATE297980T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/199,138 US6258998B1 (en) 1998-11-24 1998-11-24 Method of cloning porcine animals
PCT/US1999/026710 WO2000031237A2 (en) 1998-11-24 1999-11-12 Method of cloning porcine animals

Publications (1)

Publication Number Publication Date
ATE297980T1 true ATE297980T1 (de) 2005-07-15

Family

ID=22736378

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99958900T ATE297980T1 (de) 1998-11-24 1999-11-12 Verfahren zur klonierung von schweinen

Country Status (12)

Country Link
US (1) US6258998B1 (de)
EP (2) EP1586633A1 (de)
JP (1) JP2002530105A (de)
AT (1) ATE297980T1 (de)
AU (1) AU774430B2 (de)
BR (1) BR9915642A (de)
CA (1) CA2350831A1 (de)
DE (1) DE69925856T2 (de)
IL (1) IL143326A0 (de)
NZ (1) NZ530018A (de)
WO (1) WO2000031237A2 (de)
ZA (1) ZA200104230B (de)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6218899A (en) 1998-10-12 2000-05-01 Geron Bio-Med Limited Porcine oocytes with improved developmental competence
US6700037B2 (en) * 1998-11-24 2004-03-02 Infigen, Inc. Method of cloning porcine animals
US20040180430A1 (en) * 1999-09-07 2004-09-16 West Michael D. Methods of restoring telomere length and extending cell lifespan using nuclear transfer
US20050255596A1 (en) * 1999-09-07 2005-11-17 West Michael D Methods of repairing tandemly repeated DNA sequences and extending cell life-span nuclear transfer
AU1777301A (en) * 1999-11-19 2001-05-30 Hematech, Llc Production of ungulates, preferably bovines that produce human immunoglobulins
US7074983B2 (en) * 1999-11-19 2006-07-11 Kirin Beer Kabushiki Kaisha Transgenic bovine comprising human immunoglobulin loci and producing human immunoglobulin
US7414170B2 (en) * 1999-11-19 2008-08-19 Kirin Beer Kabushiki Kaisha Transgenic bovines capable of human antibody production
US7820878B2 (en) * 1999-11-19 2010-10-26 Kyowa Hakko Kirin Co., Ltd. Production of ungulates, preferably bovines that produce human immunoglobulins
JP2003518936A (ja) * 2000-01-04 2003-06-17 ユニバーシティ オブ コネチカット 長期間培養された雄または雌の体細胞核の、人為的に誘導される遺伝子改変を含む、除核レシピエント細胞への移植による、標的遺伝子改変を有する動物をクローニングする方法。
US20060174358A1 (en) * 2001-01-04 2006-08-03 Xiangzhong Yang Method for cloning animals with targetted genetic alterations by transfer of long-term cultured male or female somatic cell nuclei, comprising artificially-induced genetic alterations, to enucleated recipient cells
US7491534B2 (en) * 2000-12-22 2009-02-17 Kirin Holdings Kabushiki Kaisha Methods for altering cell fate to generate T-cells specific for an antigen of interest
US20020142397A1 (en) * 2000-12-22 2002-10-03 Philippe Collas Methods for altering cell fate
MXPA03005624A (es) 2000-12-22 2004-12-03 Vaurox Llc Metodos para clonar mamiferos usando cromatina reprogramada del donador o celulas donadoras.
AUPR247901A0 (en) * 2001-01-10 2001-02-01 Garelag Pty Ltd Activation of nuclear transfer embryos
WO2002074078A2 (en) 2001-01-30 2002-09-26 Rebholtz, Amy, K. Method for embryo after impregnation of the recipient female
WO2002062131A2 (en) * 2001-02-02 2002-08-15 Infigen, Inc. Method of cloning transgenic mammalian animals using pseudonuclei
WO2002074935A2 (en) 2001-03-21 2002-09-26 Geron Corporation Use of telomerase reverse transcriptase to create homozygous knockout animals
AUPR446701A0 (en) 2001-04-18 2001-05-17 Gene Stream Pty Ltd Transgenic mammals for pharmacological and toxicological studies
WO2003005810A2 (en) * 2001-07-09 2003-01-23 Alexion Pharmaceuticals Inc. Cloned non-human mammals from contact inhibited donor cells
US20030224380A1 (en) * 2001-10-25 2003-12-04 The General Hospital Corporation Genes and polymorphisms on chromosome 10 associated with Alzheimer's disease and other neurodegenerative diseases
AU2002364945A1 (en) * 2001-10-25 2003-07-09 Neurogenetics, Inc. Genes and polymorphisms on chromosome 10 associated with alzheimer's disease and other neurodegenerative diseases
US20030170678A1 (en) * 2001-10-25 2003-09-11 Neurogenetics, Inc. Genetic markers for Alzheimer's disease and methods using the same
JP2005514016A (ja) * 2001-12-21 2005-05-19 ザ キュレイターズ オブ ザ ユニバーシティ オブ ミズーリ ノックアウトブタ及びその製造方法
AU2002217606A1 (en) * 2001-12-29 2003-11-03 Woo Suk Hwang Gfp-transfected clon pig, gt knock-out clon pig and methods for production thereof
JP2003225089A (ja) * 2002-02-04 2003-08-12 National Institute Of Agrobiological Sciences ブタｒａｇ−１遺伝子およびその利用
US7736892B2 (en) 2002-02-25 2010-06-15 Kansas State University Research Foundation Cultures, products and methods using umbilical cord matrix cells
US20030161818A1 (en) * 2002-02-25 2003-08-28 Kansas State University Research Foundation Cultures, products and methods using stem cells
US20030217378A1 (en) * 2002-04-05 2003-11-20 The University Of Georgia Research Foundation, Inc Cloning using rapidly matured oocytes
US7371922B2 (en) * 2002-07-31 2008-05-13 The Board Of Trustees Of The University Of Illinois Nuclear transfer with porcine embryonic stem cells
US7462448B2 (en) * 2002-08-02 2008-12-09 Stratatech Corporation Species specific DNA detection
US7518033B2 (en) * 2002-08-02 2009-04-14 The General Hospital Corporation Methods for the production of cells and mammals with desired genetic modifications
JP2005536228A (ja) * 2002-08-21 2005-12-02 レビビコア， インコーポレイテッド 機能的α−１，３−ガラクトシルトランスフェラーゼのいかなる発現をも欠くブタ動物
US20040077077A1 (en) * 2002-10-18 2004-04-22 Xiangzhong Yang Novel methods for the production of cloned mammals, mammals cloned according to the methods, and methods of use of same
EP1563084A4 (de) * 2002-11-08 2006-03-01 Hematech Llc Transgene ungulaten mit reduzierter prionproteinaktivität und verwendungen davon
CA2548080A1 (en) * 2003-11-05 2005-05-26 University Of Pittsburgh Porcine isogloboside 3 synthase protein, cdna, genomic organization, and regulatory region
NZ549953A (en) * 2004-03-17 2010-11-26 Revivicor Inc Tissue products derived from animals lacking any expression of functional alpha 1,3 galactosyltransferase
CN101014715A (zh) * 2004-04-22 2007-08-08 麒麟麦酒株式会社 转基因动物及其用途
JP2006081542A (ja) * 2004-08-18 2006-03-30 Institute Of Physical & Chemical Research クローン哺乳動物の作成方法
US20060080746A1 (en) * 2004-09-28 2006-04-13 Viagen, Inc. Methods of embryo transfer
US20060147429A1 (en) * 2004-12-30 2006-07-06 Paul Diamond Facilitated cellular reconstitution of organs and tissues
US20060148080A1 (en) * 2004-12-30 2006-07-06 Paul Diamond Methods for supporting and producing human cells and tissues in non-human mammal hosts
NZ565711A (en) 2005-08-09 2011-10-28 Revivicor Inc Transgenic ungulates expressing a porcine CTLA4 peptide fused to an immunoglobulin
KR100807644B1 (ko) 2007-03-19 2008-02-28 충남대학교산학협력단 체외수정 수정란 및 체세포 복제 수정란의 동시 이식에의한 돼지 생산 방법
US9420770B2 (en) 2009-12-01 2016-08-23 Indiana University Research & Technology Corporation Methods of modulating thrombocytopenia and modified transgenic pigs
ES2680636T3 (es) 2011-02-14 2018-09-10 Revivicor Inc. Cerdos genéticamente modificados para xenotrasplante de xenoinjertos vascularizados y derivados de los mismos
US9781919B2 (en) 2011-06-01 2017-10-10 Inguran, Llc Compositions and methods for improving the quality of processed sperm
WO2012167151A1 (en) 2011-06-01 2012-12-06 Inguran, Llc Compositions and methods for improving the quality of processed sperm
US20140115728A1 (en) 2012-10-24 2014-04-24 A. Joseph Tector Double knockout (gt/cmah-ko) pigs, organs and tissues
DK2993234T3 (en) 2013-04-30 2019-01-07 Univ Konkuk Ind Coop Corp Vector Targeted CMP Acetylneuramic Acid Hydroxylase, Transgenic Animal for Xenotransplantation Introduced with the Vector and Method of Preparation thereof
GB201314452D0 (en) * 2013-08-13 2013-09-25 Ostara Biomedical Ltd Embryo implantation
GB201501302D0 (en) 2015-01-27 2015-03-11 Ostara Biomedical Ltd Embryo implantation
GB201517523D0 (en) 2015-10-05 2015-11-18 Ostara Biomedical Ltd Methods and compositions for managing reproduction
CA3196707A1 (en) 2020-11-20 2022-05-27 Martine Rothblatt Multi-transgenic pigs with growth hormone receptor knockout for xenotransplantation
IL311538A (en) 2021-09-20 2024-05-01 Revivicor Inc Transgenic multigene pigs comprising ten genetic alterations for xenotransplantation

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4664097A (en) 1984-05-09 1987-05-12 The Wistar Institute Of Anatomy & Biology Nuclear transplantation in the mammalian embryo by microsurgery and cell fusion
JP2874751B2 (ja) 1986-04-09 1999-03-24 ジェンザイム・コーポレーション 希望する蛋白質をミルク中へ分泌する遺伝子移植動物
US4987071A (en) 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
US4994384A (en) 1986-12-31 1991-02-19 W. R. Grace & Co.-Conn. Multiplying bovine embryos
US5346990A (en) 1987-04-08 1994-09-13 Cytogam, Inc. Sex-associated membrane proteins and methods for increasing the probability that offspring will be of a desired sex
US5021244A (en) 1988-12-06 1991-06-04 Cytogam, Inc. Sex-associated membrane antibodies and their use for increasing the probability that offspring will be of a desired sex
US5057420A (en) 1987-06-05 1991-10-15 Granada Biosciences, Inc. Bovine nuclear transplantation
US5213979A (en) 1987-12-30 1993-05-25 W. R. Grace & Co.-Conn. In vitro culture of bovine embryos
US5633076A (en) 1989-12-01 1997-05-27 Pharming Bv Method of producing a transgenic bovine or transgenic bovine embryo
US5160312A (en) 1990-02-09 1992-11-03 W. R. Grace & Co.-Conn. Cryopreservation process for direct transfer of embryos
US5096822A (en) 1990-07-26 1992-03-17 W. R. Grace & Co.- Conn. Bovine embryo medium
EP0546091B1 (de) 1990-08-29 2007-01-24 Pharming Intellectual Property BV Homologe rekombination in säugetier-zellen
US5858359A (en) 1992-02-28 1999-01-12 Bazin; Herve Antibodies for and treatment to prevent or reduce the severity of xenograft rejection
AU4225593A (en) 1992-05-01 1993-11-29 Genzyme Corporation Method for identifying transgenic preimplantation embryos
US5645986A (en) 1992-05-13 1997-07-08 Board Of Reagents, The University Of Texas System Therapy and diagnosis of conditions related to telomere length and/or telomerase activity
US5453357A (en) 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5589582A (en) 1992-10-27 1996-12-31 Biotransplant, Inc. Polynucleotides en coding porcine cytokines
US5496720A (en) 1993-02-10 1996-03-05 Susko-Parrish; Joan L. Parthenogenic oocyte activation
JP3801196B2 (ja) 1993-03-09 2006-07-26 ジェンザイム・コーポレイション 乳からの対象化合物の単離
US5523226A (en) * 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
WO1995010599A1 (en) 1993-10-15 1995-04-20 The University Of Melbourne Embryonic stem cell-like cells
US5827690A (en) 1993-12-20 1998-10-27 Genzyme Transgenics Corporatiion Transgenic production of antibodies in milk
GB9401380D0 (en) 1994-01-25 1994-03-23 Pharmaceutical Proteins Ltd Embryonic cell isolation
ATE395418T1 (de) 1994-04-13 2008-05-15 Biotransplant Inc Alpha(1,3)galactosyltransferase-negatives schwein
JPH10507906A (ja) 1994-08-19 1998-08-04 ザ ジェネラル ホスピタル コーポレイション 遺伝子工学処理したブタ細胞
GB9417831D0 (en) 1994-09-05 1994-10-26 Biotech & Biolog Scien Res Biological manipulation
GB9517780D0 (en) 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
GB9517779D0 (en) 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
US6166288A (en) 1995-09-27 2000-12-26 Nextran Inc. Method of producing transgenic animals for xenotransplantation expressing both an enzyme masking or reducing the level of the gal epitope and a complement inhibitor
AU1274797A (en) * 1995-11-29 1997-06-19 Utah State University Establishment, maintenance, and transfection of totipotent embryonic stem cells from the embryos of domestic animals
US5905042A (en) 1996-04-01 1999-05-18 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Cultured inner cell mass cell lines derived from bovine or porcine embryos
AU4984697A (en) 1996-10-11 1998-05-11 The Texas A & M University System Methods for the generation of primordial germ cells and transgenic animal species
AU745334B2 (en) * 1997-03-06 2002-03-21 Advanced Cell Technology, Inc. Method of cloning animals
AU8827998A (en) * 1997-08-14 1999-03-08 Biotransplant Incorporated Porcine totipotent cells and method for long-term culture

Also Published As

Publication number Publication date
US6258998B1 (en) 2001-07-10
WO2000031237A3 (en) 2000-11-16
IL143326A0 (en) 2002-04-21
ZA200104230B (en) 2002-08-23
DE69925856D1 (de) 2005-07-21
WO2000031237A2 (en) 2000-06-02
EP1131409A2 (de) 2001-09-12
AU774430B2 (en) 2004-06-24
CA2350831A1 (en) 2000-06-02
DE69925856T2 (de) 2006-05-11
EP1586633A1 (de) 2005-10-19
EP1131409B1 (de) 2005-06-15
BR9915642A (pt) 2001-08-07
NZ530018A (en) 2005-08-26
AU1617500A (en) 2000-06-13
JP2002530105A (ja) 2002-09-17

Similar Documents

Publication Publication Date Title
ATE297980T1 (de) Verfahren zur klonierung von schweinen
WO2002010337A3 (en) Method of cloning porcine animals
BR9808192A (pt) Processo de clonagem de animais
MEP44408A (en) Anti-il-12 antibodies, compositions, methods and uses
ME00314B (me) Anti-tnf antitijela, kompozicije, postupci i primjene
DE68919856D1 (de) Nachrichtenvermittlungselement zur übertragung von zellen unterteilt in teilzellen.
DK0713495T3 (da) Fremgangsmåder til forstærkning af den biologiske aktivitet af chemokiner
DE69430054D1 (de) Verfahren zum einbetten von tierkulturzellen
DE3882822D1 (de) Verfahren zur betriebsmittellokalisierung in rechnernetzen.
DE60126601D1 (de) Verfahren und vorrichtung zur herstellung von kohlensäurehaltigem wasser
ATE536372T1 (de) Verfahren zur hoher expression der aktiven form von lymphotoxin-beta-rezeptor-immunglobulin fusionsproteinen, und deren reinigung
DE3783865D1 (de) Elektrostatographisches verfahren zur bilderzeugung.
TR200000099T1 (tr) Üzüm için yenilenme sistemi ve kullanımı.
DE59504922D1 (de) Verfahren zur herstellung von 2-hydroxy-4-methylthiobuttersäure (mha) und deren verwendung als tierfuttermittelsupplement
DE59903972D1 (de) Verfahren zur hydrierung von aliphatischen alpha, omega-dinitrilen
ATE279441T1 (de) Verfahren zur herstellung von genetisch veränderten lymphozyten vorläuferzellen von wirbeltieren und deren gebrauch zur produktion von heterologen bindeproteinen
ATE317219T1 (de) Auf einem system basierende, automatische vorrichtung zum trainieren von tieren
DE69831730D1 (de) Verfahren zum Kühlen von Brammen mit Wasser
DE59912242D1 (de) Verfahren zur herstellung von (s,s)-benzyl-2,8-diazabicyclo(4.3.0)nonan
DE69926059D1 (de) Verfahren zur herstellung von replikas unter bewahrung des originals
DE3689276D1 (de) Verfahren zur Aktualisierung der Informationen in einem Adressenübersetzungspufferspeicher.
AR022150A1 (es) Acidos nucleicos derivados de monocitos y composiciones y metodos relacionados
FR2813502B1 (fr) Procede et installation pour ouvrir des oeufs
DE59807902D1 (de) Lösung von tetramethylammoniumhydroxid in wasser, verfahren zur herstellung der lösung und deren verwendung
DE50111689D1 (de) Verfahren zur übertragung von zusätzlichen optischen signalen im s-band

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties