ATE254627T1 - In situ herstellung von nukleosid- phosphoramiditen und ihre verwendung in der oligonukleotidsynthese - Google Patents

In situ herstellung von nukleosid- phosphoramiditen und ihre verwendung in der oligonukleotidsynthese

Info

Publication number
ATE254627T1
ATE254627T1 AT97921334T AT97921334T ATE254627T1 AT E254627 T1 ATE254627 T1 AT E254627T1 AT 97921334 T AT97921334 T AT 97921334T AT 97921334 T AT97921334 T AT 97921334T AT E254627 T1 ATE254627 T1 AT E254627T1
Authority
AT
Austria
Prior art keywords
nucleoside phosphoramidites
oligonucleotide synthesis
phosphitylating reagents
bifunctional phosphitylating
reagents according
Prior art date
Application number
AT97921334T
Other languages
English (en)
Inventor
Zhaoda Zhang
Jin-Yan Tang
Original Assignee
Avecia Biotechnology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avecia Biotechnology Inc filed Critical Avecia Biotechnology Inc
Application granted granted Critical
Publication of ATE254627T1 publication Critical patent/ATE254627T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/28—Phosphorus compounds with one or more P—C bonds
    • C07F9/48—Phosphonous acids R—P(OH)2; Thiophosphonous acids including RHP(=O)(OH); Derivatives thereof
    • C07F9/4883—Amides or esteramides thereof, e.g. RP(NR'2)2 or RP(XR')(NR''2) (X = O, S)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/553—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having one nitrogen atom as the only ring hetero atom
    • C07F9/572—Five-membered rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/6527—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07F9/6533—Six-membered rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/6558—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system
    • C07F9/65583—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system each of the hetero rings containing nitrogen as ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
AT97921334T 1996-05-03 1997-04-28 In situ herstellung von nukleosid- phosphoramiditen und ihre verwendung in der oligonukleotidsynthese ATE254627T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US64265396A 1996-05-03 1996-05-03
PCT/US1997/006777 WO1997042208A1 (en) 1996-05-03 1997-04-28 In situ preparation of nucleoside phosphoramidites and their use in synthesis of oligonucleotides

Publications (1)

Publication Number Publication Date
ATE254627T1 true ATE254627T1 (de) 2003-12-15

Family

ID=24577473

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97921334T ATE254627T1 (de) 1996-05-03 1997-04-28 In situ herstellung von nukleosid- phosphoramiditen und ihre verwendung in der oligonukleotidsynthese

Country Status (7)

Country Link
EP (1) EP0901500B1 (de)
AT (1) ATE254627T1 (de)
AU (1) AU2739797A (de)
CA (1) CA2226111C (de)
DE (1) DE69726276T2 (de)
ES (1) ES2212097T3 (de)
WO (1) WO1997042208A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7034177B2 (en) 2002-06-13 2006-04-25 Rhodia Inc Process of making phosphordiamidite compounds
WO2006094963A1 (en) * 2005-03-04 2006-09-14 Girindus Ag Synthesis of oligonucleotides

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH433292A (de) * 1963-06-07 1967-04-15 Monsanto Co Verfahren zur Herstellung von Aminophosphinen
DE1917884A1 (de) * 1968-04-08 1969-10-16 Toa Gosei Chem Ind Verfahren zur Herstellung von 1-Buten-2,3,4-tricarbonsaeureestern
JPS60500817A (ja) * 1983-06-01 1985-05-30 ベツクマン・インストルメンツ・インコ−ポレ−テツド ヌクレオシド・ホスホルアミダイト中間体の製造方法
DE3332068A1 (de) * 1983-09-06 1985-03-21 Hoechst Ag, 6230 Frankfurt Verfahren zur herstellung von nukleosidalkyl-, aralkyl- und arylphosphoniten und -phosphonaten
WO1986007362A1 (en) * 1985-06-14 1986-12-18 University Patents, Inc. Method for synthesizing deoxyoligonucleotides
JPS62212394A (ja) * 1986-03-11 1987-09-18 Nippon Zeon Co Ltd 新規なリンアミド化合物

Also Published As

Publication number Publication date
CA2226111C (en) 2005-09-20
EP0901500B1 (de) 2003-11-19
CA2226111A1 (en) 1997-11-13
EP0901500A1 (de) 1999-03-17
ES2212097T3 (es) 2004-07-16
WO1997042208A1 (en) 1997-11-13
AU2739797A (en) 1997-11-26
DE69726276D1 (de) 2003-12-24
DE69726276T2 (de) 2004-11-18

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