ATE238341T1 - Verfahren zur reinigung von rekombinanaten proteinen - Google Patents
Verfahren zur reinigung von rekombinanaten proteinenInfo
- Publication number
- ATE238341T1 ATE238341T1 AT97939436T AT97939436T ATE238341T1 AT E238341 T1 ATE238341 T1 AT E238341T1 AT 97939436 T AT97939436 T AT 97939436T AT 97939436 T AT97939436 T AT 97939436T AT E238341 T1 ATE238341 T1 AT E238341T1
- Authority
- AT
- Austria
- Prior art keywords
- proteins
- recombinate
- purifying
- sup
- metal ion
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
- B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
- B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
- B01J20/3244—Non-macromolecular compounds
- B01J20/3265—Non-macromolecular compounds with an organic functional group containing a metal, e.g. a metal affinity ligand
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J45/00—Ion-exchange in which a complex or a chelate is formed; Use of material as complex or chelate forming ion-exchangers; Treatment of material for improving the complex or chelate forming ion-exchange properties
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
- C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
- C12N9/003—Dihydrofolate reductase [DHFR] (1.5.1.3)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Toxicology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- General Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/698,747 US5962641A (en) | 1996-08-16 | 1996-08-16 | Method for purification of recombinant proteins |
PCT/US1997/014487 WO1998006739A1 (en) | 1996-08-16 | 1997-08-18 | Method for purification of recombinant proteins |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE238341T1 true ATE238341T1 (de) | 2003-05-15 |
Family
ID=24806511
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT97939436T ATE238341T1 (de) | 1996-08-16 | 1997-08-18 | Verfahren zur reinigung von rekombinanaten proteinen |
Country Status (8)
Country | Link |
---|---|
US (4) | US5962641A (de) |
EP (1) | EP0935606B1 (de) |
JP (1) | JP3946772B2 (de) |
AT (1) | ATE238341T1 (de) |
DE (1) | DE69721289T2 (de) |
DK (1) | DK0935606T3 (de) |
ES (1) | ES2195168T3 (de) |
WO (1) | WO1998006739A1 (de) |
Families Citing this family (40)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000024910A1 (en) * | 1998-10-28 | 2000-05-04 | Smithkline Beecham Plc | Process of purification of low density lipoprotein associated phospholipase a2 using immobilized metal affinity chromatography |
US6232083B1 (en) * | 1999-03-12 | 2001-05-15 | The Research Foundation Of State University Of New York | Methods and compositions for screening cloned proteins |
GB2367818A (en) * | 2000-10-11 | 2002-04-17 | Kalibrant Ltd | A linker and a method of chemical synthesis |
DE10106339A1 (de) * | 2001-02-09 | 2002-08-22 | Hans-Joachim Mueller | Verfahren zum Hochdurchsatz-Screening von Kandidaten-Verbindungen über die Hochdurchsatz-Direktsynthese von rekombinanten Polypeptiden |
US20040180415A1 (en) * | 2001-05-15 | 2004-09-16 | Tchaga Grigoriy S. | Methods and compositions for protein purification |
JP4283666B2 (ja) * | 2001-06-21 | 2009-06-24 | クローンテック ラボラトリーズ インク. | 水溶性ポリマー金属イオンアフィニティ組成物、およびその使用方法 |
US20040126890A1 (en) * | 2002-06-10 | 2004-07-01 | Gjerde Douglas T. | Biomolecule open channel solid phase extraction systems and methods |
US7151167B2 (en) * | 2002-06-10 | 2006-12-19 | Phynexus, Inc. | Open channel solid phase extraction systems and methods |
US7879621B2 (en) * | 2003-05-08 | 2011-02-01 | Phynexus, Inc. | Open channel solid phase extraction systems and methods |
US20040224329A1 (en) * | 2003-05-08 | 2004-11-11 | Gjerde Douglas T. | Three-dimensional solid phase extraction surfaces |
US7122640B2 (en) * | 2002-06-10 | 2006-10-17 | Phynexus, Inc. | Open channel solid phase extraction systems and methods |
GB0215185D0 (en) | 2002-07-01 | 2002-08-07 | Genovision As | Binding a target substance |
CA2498341A1 (en) * | 2002-10-18 | 2004-04-29 | Promega Corporation | Composition for separating molecules |
US20040241721A1 (en) * | 2003-05-08 | 2004-12-02 | Gjerde Douglas T. | Open channel solid phase extraction systems and methods |
US20060189797A1 (en) * | 2003-06-27 | 2006-08-24 | Pal Songe | Magnetic polymer particles |
US7115397B2 (en) * | 2003-09-12 | 2006-10-03 | Promega Corporation | Methods and kits for purifying his-tagged proteins |
WO2005037858A2 (en) * | 2003-10-16 | 2005-04-28 | University Of Massachusetts | Template-directed assembly of receptor signaling complexes |
GB0406015D0 (en) * | 2004-03-17 | 2004-04-21 | Dynal Biotech Asa | Improvements in magnetic polymer particles |
US20050272116A1 (en) * | 2004-06-04 | 2005-12-08 | Clemson University | Polymer substrate for recombinant protein purification and environmental remediation |
WO2006042263A2 (en) * | 2004-10-12 | 2006-04-20 | Clontech Laboratories, Inc. | Phosphoprotein affinity resins and methods for making and using the same |
US20070196833A1 (en) * | 2005-04-21 | 2007-08-23 | Gjerde Douglas T | Open channel solid phase extraction systems and methods |
US20070161785A1 (en) * | 2005-12-05 | 2007-07-12 | Clontech Laboratories, Inc. | High density metal ion affinity compositions and methods for making and using the same |
WO2007139470A1 (en) * | 2006-05-30 | 2007-12-06 | Ge Healthcare Bio-Sciences Ab | A method of preparing an immobilised metal ion chromatography adsorbent and methods of purifying proteins, peptides or polynucleotides. |
WO2008002656A2 (en) * | 2006-06-28 | 2008-01-03 | P.A. Technologies Llc | Methods and materials for in vitro analysis and/or use of membrane-associated proteins, portions thereof or variants thereof |
EP2183049B2 (de) | 2007-08-06 | 2019-03-13 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Verwendung von immobilisierte chelatbildner zur aufreinigung von rekombinanten proteinen durch metallchelatchromatographie und verfahren zur aufreinigung von rekombinanten proteinen |
EP2022561A1 (de) * | 2007-08-06 | 2009-02-11 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Immobilisierung von chelatbildenden Gruppen für immobilisierte Metallionenchromatographie (IMAC) |
JP2009073819A (ja) | 2007-08-29 | 2009-04-09 | Fujifilm Corp | 生理活性物質の精製方法 |
DE102008030142A1 (de) * | 2008-06-27 | 2009-12-31 | Qiagen Gmbh | Verfahren zur Proteinaufreinigung unter denaturierenden Bedingungen |
CN101323454B (zh) * | 2008-07-28 | 2010-09-08 | 陕西师范大学 | 表面螯合金属离子的磁性二氧化硅微球制备方法 |
WO2010036520A1 (en) * | 2008-09-26 | 2010-04-01 | Wisconsin Alumni Research Foundation | Mesoporous metal oxide materials for phosphoproteomics |
JP2012522057A (ja) | 2009-03-30 | 2012-09-20 | ジヨンソン・アンド・ジヨンソン・コンシユーマー・カンパニーズ・インコーポレーテツド | 化粧用剤の送達のためのペプチドに基づく系 |
US8481678B2 (en) | 2009-03-30 | 2013-07-09 | E I Du Pont De Nemours And Company | Peptide-based tooth whitening reagents |
ES2534562T3 (es) * | 2010-05-19 | 2015-04-24 | F. Hoffmann-La Roche Ag | Procedimiento de cromatografía de interacción hidrófoba |
CN102127164B (zh) | 2010-12-20 | 2013-01-30 | 武汉禾元生物科技有限公司 | 一种从水稻种子中提取重组人血清白蛋白的方法 |
CN102532254B (zh) * | 2010-12-24 | 2015-06-24 | 武汉禾元生物科技股份有限公司 | 一种从水稻种子中分离纯化重组人血清白蛋白的方法 |
CN103880947B (zh) | 2012-12-21 | 2016-01-13 | 武汉禾元生物科技股份有限公司 | 一种分离纯化高纯度重组人血清白蛋白的层析方法 |
ES2465715B1 (es) | 2014-02-14 | 2015-03-18 | Universitat Autónoma De Barcelona | Péptido, péptido magnético y método para detectar la enfermedad celíaca |
CN105085607B (zh) * | 2015-09-30 | 2018-05-29 | 河南科技大学 | 一种利用蛋白变性分离特定金属结合蛋白的方法 |
CN105085606B (zh) * | 2015-09-30 | 2018-05-29 | 河南科技大学 | 一种利用铜螯合磁珠分离铜蛋白质组的方法 |
CN113993548A (zh) * | 2019-06-18 | 2022-01-28 | 马克思—普朗克科学促进协会公司 | 标记物和/或载体与靶分子例如带His-标签的蛋白质通过金属配合物试剂的位点特异性动力学惰性缀合 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3853708A (en) * | 1970-01-23 | 1974-12-10 | Exploaterings Ab Tbf | Coupling biologically active substances to oxirane-containing polymers |
US5047513A (en) * | 1986-07-10 | 1991-09-10 | Hoffmann-La Roche Inc. | Metal chelate resins |
CA1304886C (en) * | 1986-07-10 | 1992-07-07 | Heinz Dobeli | Metal chelate resins |
-
1996
- 1996-08-16 US US08/698,747 patent/US5962641A/en not_active Expired - Lifetime
-
1997
- 1997-08-18 US US08/912,406 patent/US6242581B1/en not_active Expired - Lifetime
- 1997-08-18 WO PCT/US1997/014487 patent/WO1998006739A1/en active IP Right Grant
- 1997-08-18 EP EP97939436A patent/EP0935606B1/de not_active Expired - Lifetime
- 1997-08-18 AT AT97939436T patent/ATE238341T1/de not_active IP Right Cessation
- 1997-08-18 ES ES97939436T patent/ES2195168T3/es not_active Expired - Lifetime
- 1997-08-18 JP JP51009798A patent/JP3946772B2/ja not_active Expired - Lifetime
- 1997-08-18 DK DK97939436T patent/DK0935606T3/da active
- 1997-08-18 DE DE69721289T patent/DE69721289T2/de not_active Expired - Lifetime
-
2001
- 2001-04-19 US US09/839,696 patent/US7193041B2/en not_active Expired - Lifetime
-
2006
- 2006-01-19 US US11/336,748 patent/US20060122378A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US20020143158A1 (en) | 2002-10-03 |
US5962641A (en) | 1999-10-05 |
JP3946772B2 (ja) | 2007-07-18 |
JP2001506968A (ja) | 2001-05-29 |
DE69721289T2 (de) | 2004-01-29 |
WO1998006739A1 (en) | 1998-02-19 |
US6242581B1 (en) | 2001-06-05 |
US20060122378A1 (en) | 2006-06-08 |
DE69721289D1 (de) | 2003-05-28 |
EP0935606A1 (de) | 1999-08-18 |
WO1998006739A8 (en) | 1998-05-07 |
EP0935606B1 (de) | 2003-04-23 |
US7193041B2 (en) | 2007-03-20 |
DK0935606T3 (da) | 2003-08-11 |
ES2195168T3 (es) | 2003-12-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |