ATE233826T1 - Verbesserte erkennung von spezifischen nukleinsäuren - Google Patents

Verbesserte erkennung von spezifischen nukleinsäuren

Info

Publication number
ATE233826T1
ATE233826T1 AT97921072T AT97921072T ATE233826T1 AT E233826 T1 ATE233826 T1 AT E233826T1 AT 97921072 T AT97921072 T AT 97921072T AT 97921072 T AT97921072 T AT 97921072T AT E233826 T1 ATE233826 T1 AT E233826T1
Authority
AT
Austria
Prior art keywords
nucleic acids
specific nucleic
improved detection
target
sequence
Prior art date
Application number
AT97921072T
Other languages
German (de)
English (en)
Inventor
Ulf Landegren
Original Assignee
Ulf Landegren
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ulf Landegren filed Critical Ulf Landegren
Application granted granted Critical
Publication of ATE233826T1 publication Critical patent/ATE233826T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT97921072T 1996-04-30 1997-04-30 Verbesserte erkennung von spezifischen nukleinsäuren ATE233826T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE9601676A SE9601676D0 (sv) 1996-04-30 1996-04-30 Improved probing of specific mucleic acids
PCT/SE1997/000737 WO1997041254A1 (en) 1996-04-30 1997-04-30 Improved probing of specific nucleic acids

Publications (1)

Publication Number Publication Date
ATE233826T1 true ATE233826T1 (de) 2003-03-15

Family

ID=20402429

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97921072T ATE233826T1 (de) 1996-04-30 1997-04-30 Verbesserte erkennung von spezifischen nukleinsäuren

Country Status (8)

Country Link
US (2) US20010007742A1 (Direct)
EP (1) EP0951565B1 (Direct)
JP (1) JP2000508920A (Direct)
AT (1) ATE233826T1 (Direct)
CA (1) CA2252817C (Direct)
DE (1) DE69719557T2 (Direct)
SE (1) SE9601676D0 (Direct)
WO (1) WO1997041254A1 (Direct)

Families Citing this family (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US20060141475A1 (en) * 2002-11-19 2006-06-29 Applera Corporation Polynucleotide sequence detection assays and analysis
WO2004046344A2 (en) * 2002-11-19 2004-06-03 Applera Corporation Polynucleotide sequence detection assays
US7799525B2 (en) 2003-06-17 2010-09-21 Human Genetic Signatures Pty Ltd. Methods for genome amplification
CA2537810C (en) * 2003-09-04 2012-12-18 Human Genetic Signatures Pty Ltd Nucleic acid detection assay
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
WO2005113804A1 (en) * 2004-05-20 2005-12-01 Trillion Genomics Limited Use of mass labelled probes to detect target nucleic acids using mass spectrometry
EP1794173B1 (en) 2004-09-10 2010-08-04 Human Genetic Signatures PTY Ltd Amplification blocker comprising intercalating nucleic acids (ina) containing intercalating pseudonucleotides (ipn)
WO2006058393A1 (en) 2004-12-03 2006-06-08 Human Genetic Signatures Pty Ltd Methods for simplifying microbial nucleic acids by chemical modification of cytosines
WO2006125267A1 (en) 2005-05-26 2006-11-30 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
CN101292046B (zh) 2005-09-14 2013-03-13 人类遗传标记控股有限公司 健康状态的测定法
EP1879377A3 (en) * 2006-07-13 2010-06-16 Panasonic Corporation Portable Device
CN101918595A (zh) 2007-11-27 2010-12-15 人类遗传标记控股有限公司 用于扩增和复制亚硫酸氢盐修饰的核酸的酶
WO2009121021A1 (en) * 2008-03-28 2009-10-01 Life Technologies Corporation Degradable arrays for preventing reuse
ES2637411T3 (es) 2008-12-01 2017-10-13 Laboratory Corporation Of America Holdings Métodos y ensayos para medir p95 Y/O p95 en una muestra y anticuerpos específicos para p95
CA2749846C (en) 2009-01-15 2018-08-07 Laboratory Corporation Of America Holdings Methods of determining patient response by measurement of her-3
WO2010083463A1 (en) * 2009-01-15 2010-07-22 Laboratory Corporation Of America Holdings Methods of determining patient response by measurement of her-2 expression
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
AU2010242073C1 (en) 2009-04-30 2015-12-24 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
EP2504448B1 (en) * 2009-11-25 2016-10-19 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
AU2012214312A1 (en) 2011-02-09 2013-08-22 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
AU2012219132B2 (en) 2011-02-15 2016-05-12 Mats Nilsson Bernitz Method for localized in situ detection of mRNA
US9556473B2 (en) 2011-02-15 2017-01-31 Leica Biosystems Newcastle Ltd Methods for identifying nucleic acid sequences
US8759036B2 (en) 2011-03-21 2014-06-24 Affymetrix, Inc. Methods for synthesizing pools of probes
US8932819B2 (en) 2011-07-06 2015-01-13 Affymetrix, Inc. Assays for affinity profiling of nucleic acid binding proteins
ES2613743T3 (es) 2011-09-07 2017-05-25 Human Genetic Signatures Pty Ltd Ensayo de detección molecular
CA2852665A1 (en) 2011-10-17 2013-04-25 Good Start Genetics, Inc. Analysis methods
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
CN105392895B (zh) 2013-03-13 2019-07-26 中尺度技术有限责任公司 改进的测定方法
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
EP2971159B1 (en) 2013-03-14 2019-05-08 Molecular Loop Biosolutions, LLC Methods for analyzing nucleic acids
CN105209532B (zh) 2013-03-15 2017-11-28 路博润先进材料公司 不含重金属的cpvc组合物
EP3005200A2 (en) 2013-06-03 2016-04-13 Good Start Genetics, Inc. Methods and systems for storing sequence read data
EP3024948B1 (en) 2013-07-25 2020-01-15 Bio-rad Laboratories, Inc. Genetic assays for detecting viral recombination rate
US11041203B2 (en) 2013-10-18 2021-06-22 Molecular Loop Biosolutions, Inc. Methods for assessing a genomic region of a subject
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
US11053548B2 (en) 2014-05-12 2021-07-06 Good Start Genetics, Inc. Methods for detecting aneuploidy
CN112239774B (zh) 2014-05-15 2024-12-03 中尺度技术有限责任公司 改进的测定方法
CN106661606B (zh) 2014-06-13 2021-12-21 Q-莱纳公司 用于检测和表征微生物的方法
US20160048608A1 (en) 2014-08-15 2016-02-18 Good Start Genetics, Inc. Systems and methods for genetic analysis
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
US10429399B2 (en) 2014-09-24 2019-10-01 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
EP4098753A1 (en) 2014-10-23 2022-12-07 Ricardo Mancebo Reagents and methods for isothermal chain reaction
EP4095261B1 (en) 2015-01-06 2025-05-28 Molecular Loop Biosciences, Inc. Screening for structural variants
GB201507026D0 (en) 2015-04-24 2015-06-10 Linea Ab Q Medical sample transportation container
EP3329010B1 (en) 2015-07-29 2019-07-10 Progenity, Inc. Nucleic acids and methods for detecting chromosomal abnormalities
EP3377647B1 (en) 2015-11-16 2020-03-11 Progenity, Inc. Nucleic acids and methods for detecting methylation status
GB2554767A (en) 2016-04-21 2018-04-11 Q Linea Ab Detecting and characterising a microorganism
EP4614155A3 (en) 2016-12-01 2025-10-15 Nautilus Subsidiary, Inc. Methods of assaying proteins
WO2018183607A1 (en) 2017-03-30 2018-10-04 Pioneer Hi-Bred International, Inc. Methods of identifying and characterizing gene editing variations in nucleic acids
BR112020013252A2 (pt) 2017-12-29 2020-12-01 Nautilus Biotechnology, Inc. abordagens de decodificação para a identificação de proteína
EP4647508A3 (en) 2018-04-02 2026-01-21 Enumera Molecular, Inc. Methods, systems, and compositions for counting nucleic acid molecules
CN114144422B (zh) 2019-03-01 2025-03-07 中尺度技术有限责任公司 用于免疫方法的被电化学发光标记的探针、使用这类探针的方法和包含这类探针的试剂盒
EP3947718A4 (en) 2019-04-02 2022-12-21 Enumera Molecular, Inc. METHODS, SYSTEMS AND COMPOSITIONS FOR COUNTING NUCLEIC ACID MOLECULES
JP7727628B2 (ja) 2019-12-03 2025-08-21 アラマー バイオサイエンシーズ， インク． 核酸結合免疫サンドイッチアッセイ（ｎｕｌｉｓａ）
GB202004469D0 (en) 2020-03-27 2020-05-13 Olink Proteomics Ab Controls for proximity detection assays
EP4165549A1 (en) 2020-06-11 2023-04-19 Nautilus Biotechnology, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
AU2021345359A1 (en) 2020-09-21 2023-05-11 Enumera Molecular, Inc. Compositions and methods for isolation of cell-free dna
CA3203106A1 (en) 2021-01-20 2022-07-28 Parag Mallick Systems and methods for biomolecule quantitation
AU2022209365A1 (en) 2021-01-21 2023-07-20 Nautilus Subsidiary, Inc. Systems and methods for biomolecule preparation
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
US12529049B2 (en) 2021-09-09 2026-01-20 Nautilus Subsidiary, Inc. Characterization and localization of protein modifications
WO2023049073A1 (en) 2021-09-22 2023-03-30 Nautilus Biotechnology, Inc. Methods and systems for determining polypeptide interactions
US20230149883A1 (en) 2021-11-03 2023-05-18 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
CA3245990A1 (en) 2022-03-29 2023-10-05 Nautilus Subsidiary, Inc. INTEGRATED NETWORKS FOR SINGLE-ANALYTE PROCESSES
WO2023212490A1 (en) 2022-04-25 2023-11-02 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
WO2023250364A1 (en) 2022-06-21 2023-12-28 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
WO2024059655A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
EP4630816A1 (en) 2022-12-09 2025-10-15 Nautilus Subsidiary, Inc. A method comprising performing on a single-analyte array at least 50 cycles of a process
EP4634285A1 (en) 2022-12-15 2025-10-22 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
WO2024206122A1 (en) 2023-03-24 2024-10-03 Nautilus Subsidiary, Inc. Improved transfer of nanoparticles to array surfaces
WO2024243237A1 (en) 2023-05-24 2024-11-28 Nautilus Subsidiary, Inc. Systems and methods for machine vision
US20250066841A1 (en) 2023-08-24 2025-02-27 Nautilus Subsidiary, Inc. Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions
US20250189519A1 (en) 2023-12-11 2025-06-12 Nautilus Subsidiary, Inc. Methods and compositions of particle-based arrays
WO2025137166A1 (en) 2023-12-20 2025-06-26 Nautilus Subsidiary, Inc. Fluidic devices for single-analyte assays
WO2025199236A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
WO2025212338A1 (en) 2024-04-01 2025-10-09 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
SE9400522D0 (sv) * 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5643724A (en) * 1994-06-06 1997-07-01 Roche Molecular Systems, Inc. Methods and reagents for Glycophorin A typing
GB9414934D0 (en) * 1994-07-25 1994-09-14 Raggio Italgene Spa Method and reagents for the detection of target nuceic acid sequences
WO1997003210A1 (en) 1995-07-11 1997-01-30 FORFÁS (trading as BIORESEARCH IRELAND) Glycosylase mediated detection of nucleotide sequences at candidate loci

Also Published As

Publication number Publication date
US20010007742A1 (en) 2001-07-12
JP2000508920A (ja) 2000-07-18
WO1997041254A1 (en) 1997-11-06
US7351528B2 (en) 2008-04-01
DE69719557D1 (de) 2003-04-10
SE9601676D0 (sv) 1996-04-30
DE69719557T2 (de) 2004-02-26
CA2252817C (en) 2009-07-07
EP0951565A1 (en) 1999-10-27
EP0951565B1 (en) 2003-03-05
US20020102592A1 (en) 2002-08-01
CA2252817A1 (en) 1997-11-06

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