CA2252817C - Improved probing of specific nucleic acids - Google Patents

Improved probing of specific nucleic acids Download PDF

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Publication number
CA2252817C
CA2252817C CA002252817A CA2252817A CA2252817C CA 2252817 C CA2252817 C CA 2252817C CA 002252817 A CA002252817 A CA 002252817A CA 2252817 A CA2252817 A CA 2252817A CA 2252817 C CA2252817 C CA 2252817C
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CA
Canada
Prior art keywords
probe
solid phase
nucleic acid
padlock
target nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002252817A
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English (en)
French (fr)
Other versions
CA2252817A1 (en
Inventor
Ulf Landegren
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olink Holding AB
Original Assignee
Olink AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olink AB filed Critical Olink AB
Publication of CA2252817A1 publication Critical patent/CA2252817A1/en
Application granted granted Critical
Publication of CA2252817C publication Critical patent/CA2252817C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CA002252817A 1996-04-30 1997-04-30 Improved probing of specific nucleic acids Expired - Lifetime CA2252817C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE9601676A SE9601676D0 (sv) 1996-04-30 1996-04-30 Improved probing of specific mucleic acids
SE9601676-1 1996-04-30
PCT/SE1997/000737 WO1997041254A1 (en) 1996-04-30 1997-04-30 Improved probing of specific nucleic acids

Publications (2)

Publication Number Publication Date
CA2252817A1 CA2252817A1 (en) 1997-11-06
CA2252817C true CA2252817C (en) 2009-07-07

Family

ID=20402429

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002252817A Expired - Lifetime CA2252817C (en) 1996-04-30 1997-04-30 Improved probing of specific nucleic acids

Country Status (8)

Country Link
US (2) US20010007742A1 (Direct)
EP (1) EP0951565B1 (Direct)
JP (1) JP2000508920A (Direct)
AT (1) ATE233826T1 (Direct)
CA (1) CA2252817C (Direct)
DE (1) DE69719557T2 (Direct)
SE (1) SE9601676D0 (Direct)
WO (1) WO1997041254A1 (Direct)

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Also Published As

Publication number Publication date
US20010007742A1 (en) 2001-07-12
JP2000508920A (ja) 2000-07-18
WO1997041254A1 (en) 1997-11-06
US7351528B2 (en) 2008-04-01
DE69719557D1 (de) 2003-04-10
SE9601676D0 (sv) 1996-04-30
ATE233826T1 (de) 2003-03-15
DE69719557T2 (de) 2004-02-26
EP0951565A1 (en) 1999-10-27
EP0951565B1 (en) 2003-03-05
US20020102592A1 (en) 2002-08-01
CA2252817A1 (en) 1997-11-06

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Effective date: 20170501