ATE231875T1 - Isolierung von nukleinsäure - Google Patents

Isolierung von nukleinsäure

Info

Publication number
ATE231875T1
ATE231875T1 AT97915528T AT97915528T ATE231875T1 AT E231875 T1 ATE231875 T1 AT E231875T1 AT 97915528 T AT97915528 T AT 97915528T AT 97915528 T AT97915528 T AT 97915528T AT E231875 T1 ATE231875 T1 AT E231875T1
Authority
AT
Austria
Prior art keywords
nucleic acid
adsorption
phase
ionic strength
polymer
Prior art date
Application number
AT97915528T
Other languages
English (en)
Inventor
Philippe Cros
Abdelhamid Elaissari
Claude Mabilat
Christian Pichot
Marc Rodrigue
Lise Santoro
Original Assignee
Bio Merieux
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Merieux filed Critical Bio Merieux
Application granted granted Critical
Publication of ATE231875T1 publication Critical patent/ATE231875T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
AT97915528T 1996-03-20 1997-03-20 Isolierung von nukleinsäure ATE231875T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR9603753 1996-03-20
FR9604691 1996-04-09
PCT/FR1997/000496 WO1997034909A1 (fr) 1996-03-20 1997-03-20 Isolement de l'acide nucleique

Publications (1)

Publication Number Publication Date
ATE231875T1 true ATE231875T1 (de) 2003-02-15

Family

ID=26232612

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97915528T ATE231875T1 (de) 1996-03-20 1997-03-20 Isolierung von nukleinsäure

Country Status (8)

Country Link
US (1) US6737235B1 (de)
EP (1) EP0842184B1 (de)
JP (1) JP4693944B2 (de)
AT (1) ATE231875T1 (de)
CA (1) CA2222192A1 (de)
DE (1) DE69718752T2 (de)
ES (1) ES2188926T3 (de)
WO (1) WO1997034909A1 (de)

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2965131B2 (ja) 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
CA2214495C (en) * 1996-09-25 2002-02-05 Daniel L. Woodard Hydrated zirconium silicate composition for purification of nucleic acids
JP3551293B2 (ja) 1998-02-02 2004-08-04 東洋紡績株式会社 核酸抽出装置
DE19907023A1 (de) * 1999-02-19 2000-08-24 Bayer Ag Verfahren zur Isolierung von Nucleinsäuren
FR2800635B1 (fr) 1999-11-05 2002-07-26 Bio Merieux Nanospheres composites, conjugues derives, procede de preparation et leurs utilisations
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
US20040121335A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents associated with host versus graft and graft versus host rejections
US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US8073627B2 (en) 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
FR2829580B1 (fr) 2001-09-07 2004-02-13 Bio Merieux Procede de lecture, de detection ou de quantification, hybrides ou complexes utilises dans ce procede et biopuce mettant en oeuvre ledit procede
JP3890556B2 (ja) * 2001-11-28 2007-03-07 富士フイルム株式会社 核酸の分離精製方法及び核酸分離精製ユニット
FR2837401B1 (fr) * 2002-03-25 2004-12-03 Commissariat Energie Atomique Procede de concentration de macromolecules ou agglomerats de molecules ou particules
JP2003339379A (ja) * 2002-05-24 2003-12-02 Tosoh Corp 核酸分離方法
EP1578399A4 (de) 2002-12-06 2007-11-28 Isis Pharmaceuticals Inc Verfahren für die rasche identifikation von pathogenen bei tier und mensch
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US20050112625A1 (en) * 2003-08-22 2005-05-26 Konica Minolta Medical & Graphic, Inc. DNA amplification method
US8288523B2 (en) 2003-09-11 2012-10-16 Ibis Biosciences, Inc. Compositions for use in identification of bacteria
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
WO2005117270A2 (en) 2004-05-24 2005-12-08 Isis Pharmaceuticals, Inc. Mass spectrometry with selective ion filtration by digital thresholding
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
WO2006135400A2 (en) 2004-08-24 2006-12-21 Isis Pharmaceuticals, Inc. Methods for rapid identification of recombinant organisms
EP1869180B1 (de) 2005-03-03 2013-02-20 Ibis Biosciences, Inc. Zusammensetzung zur Verwendung bei der Identifikation von Polyomaviren
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
KR100657957B1 (ko) * 2005-04-12 2006-12-14 삼성전자주식회사 아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법 및 상기방법에 사용될 수 있는 핵산 분리용 고상 물질
US20060234251A1 (en) * 2005-04-19 2006-10-19 Lumigen, Inc. Methods of enhancing isolation of RNA from biological samples
DE102006020463B4 (de) * 2005-05-24 2013-05-23 Sartorius Stedim Biotech Gmbh Verfahren zum Entfernen von Nukleinsäuren aus einer Flüssigkeit, Verfahren zur Herstellung von Adsorberelementen und Verwendung von Membranadsorbermaterial-Verschnitt
US8026084B2 (en) 2005-07-21 2011-09-27 Ibis Biosciences, Inc. Methods for rapid identification and quantitation of nucleic acid variants
US20070185322A1 (en) * 2006-02-08 2007-08-09 Nexgen Diagnostics Llc Methods of extracting RNA
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
FR2902799B1 (fr) 2006-06-27 2012-10-26 Millipore Corp Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
US9149473B2 (en) 2006-09-14 2015-10-06 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
CA2614069C (en) 2006-12-11 2016-05-03 F. Hoffmann-La Roche Ag Nucleic acid isolation using polidocanol and derivatives
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
WO2008079280A1 (en) 2006-12-21 2008-07-03 Millipore Corporation Purification of proteins
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
WO2008104002A2 (en) 2007-02-23 2008-08-28 Ibis Biosciences, Inc. Methods for rapid forensic dna analysis
WO2008151023A2 (en) 2007-06-01 2008-12-11 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
US8999702B2 (en) 2008-06-11 2015-04-07 Emd Millipore Corporation Stirred tank bioreactor
US8550694B2 (en) 2008-09-16 2013-10-08 Ibis Biosciences, Inc. Mixing cartridges, mixing stations, and related kits, systems, and methods
WO2010033627A2 (en) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
US8534447B2 (en) 2008-09-16 2013-09-17 Ibis Biosciences, Inc. Microplate handling systems and related computer program products and methods
JP2012511929A (ja) 2008-12-16 2012-05-31 イー・エム・デイー・ミリポア・コーポレイシヨン 攪拌タンク反応器及び方法
WO2010093943A1 (en) 2009-02-12 2010-08-19 Ibis Biosciences, Inc. Ionization probe assemblies
WO2011008971A1 (en) 2009-07-17 2011-01-20 Ibis Biosciences, Inc. Lift and mount apparatus
US9194877B2 (en) 2009-07-17 2015-11-24 Ibis Biosciences, Inc. Systems for bioagent indentification
EP2488656B1 (de) 2009-10-15 2015-06-03 Ibis Biosciences, Inc. Mehrfache verschiebungsverstärkung
EP2571903B1 (de) 2010-05-17 2019-09-04 EMD Millipore Corporation Stimulusreaktive polymere zur aufreinigung von biomolekülen
CN106062210A (zh) * 2013-12-23 2016-10-26 昆士兰大学 核酸检测方法和试剂盒
EP3505934A1 (de) * 2017-12-29 2019-07-03 Blink AG Sensorkörper zur bindung und/oder anreicherung und/oder detektion eines analyten in einer probe
JP7031900B2 (ja) * 2020-11-09 2022-03-08 国立大学法人信州大学 階層構造粒子および階層構造粒子の製造方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1279307C (en) * 1984-05-07 1991-01-22 Hiroshi Itoh High molecular composite material
US4767700A (en) * 1985-02-15 1988-08-30 Beckman Research Institute Of The City Of Hope Detection of particular nucleotide sequences
US4912032A (en) * 1986-04-17 1990-03-27 Genetec Systems Corporation Methods for selectively reacting ligands immobilized within a temperature-sensitive polymer gel
US5206136A (en) * 1986-11-19 1993-04-27 Genetic Systems Corporation Rapid membrane affinity concentration assays
DE3717209A1 (de) 1987-05-22 1988-12-01 Diagen Inst Molekularbio Mittel zur selektiven adsorption von biopolymeren
JP2739068B2 (ja) * 1988-09-24 1998-04-08 春馬 川口 Ｄｎａ固定ミクロスフェア及びそれを用いるｄｎａ転写制御因子精製法
EP0366241A3 (de) 1988-10-04 1990-05-23 Fisher Scientific Company Vorrichtung mit adsorbierender Oberfläche und Herstellungsverfahren
US4997932A (en) * 1989-11-13 1991-03-05 Boehringer Mannheim Corporation Method and kit for purifying nucleic acids
TW226382B (de) * 1990-09-21 1994-07-11 Takeda Pharm Industry Co Ltd
AU8951191A (en) * 1990-10-29 1992-05-26 Dekalb Plant Genetics Isolation of biological materials using magnetic particles
US5225062A (en) * 1991-02-27 1993-07-06 W. R. Grace & Co. -Conn. Electrophoretic gel for separation and recovery of substances and its use
US5569364A (en) * 1992-11-05 1996-10-29 Soane Biosciences, Inc. Separation media for electrophoresis
WO1995004140A1 (en) 1993-07-28 1995-02-09 Akzo Nobel N.V. Process for isolating nucleic acid from gram positive microorganisms
US5434270A (en) * 1994-09-15 1995-07-18 Eastman Kodak Company Weakly basic polymerizable monomers and polymers prepared therefrom

Also Published As

Publication number Publication date
EP0842184A1 (de) 1998-05-20
CA2222192A1 (fr) 1997-09-25
JPH11505426A (ja) 1999-05-21
DE69718752T2 (de) 2003-12-24
EP0842184B1 (de) 2003-01-29
ES2188926T3 (es) 2003-07-01
WO1997034909A1 (fr) 1997-09-25
JP4693944B2 (ja) 2011-06-01
US6737235B1 (en) 2004-05-18
DE69718752D1 (de) 2003-03-06

Similar Documents

Publication Publication Date Title
ATE231875T1 (de) Isolierung von nukleinsäure
AU724288B2 (en) Preformed polymer coating process and product
US5728296A (en) Selective recognition of solutes in chromatographic media by artificially created affinity
DE50104019D1 (de) Verfahren zur agglomeration dispergierter kautschuke
DE69839133D1 (de) Isolierung von Nukleinsäuren
EP0385587B1 (de) Ionenaustauscherzusammenstellung mit einer Harzbindung an das Dispergiermittel und Verfahren zu deren Herstellung
EP2302386B1 (de) Western-Blot Immunoassay unter Verwendung von hydrophile Membran mit hoher Proteinbindung und geringer Fluoreszenz
JPH02110139A (ja) 帯電改質された疎水性膜材料及びその製造方法
WO1999045374A3 (en) Purification and detection processes using reversible affinity electrophoresis
Vaidya et al. Creating a macromolecular receptor by affinity imprinting
IE822645L (en) Method for preparing an analytical element preventing¹reagent migration
Dulman et al. Adsorption of anionic textile dye Acid Green 9 from aqueous solution onto weak or strong base anion exchangers
EP0697906A1 (de) Künstliche rezeptoren, antikörper und enzyme
Özdemir et al. Boronic acid–functionalized HEMA‐based gels for nucleotide adsorption
AU4292700A (en) Microscale total analysis system
CA2748710A1 (en) Molecularly-imprinted polymeric materials for visual detection of explosives
JP4024209B2 (ja) イオン交換クロマトグラフィー用アルカリ安定型親水性吸着剤
Fontanals et al. Synthesis of hydrophilic sorbents from N‐vinylimidazole/divinylbenzene and the evaluation of their sorption properties in the solid‐phase extraction of polar compounds
US5160626A (en) Blotting methods using polyaldehyde activated membranes
US4992172A (en) Blotting methods using polyaldehyde activated membranes
Denizli et al. Novel dye-attached macroporous films for cadmium, zinc and lead sorption: Alkali Blue 6B-attached macroporous poly (2-hydroxyethyl methacrylate)
JP3062745B1 (ja) タンパク質を選択的に吸着脱離できる材料及びその製造方法
Yao et al. Polyacrylamide gel beads for the recognition of staphylococcal enterotoxin B
Akiyama et al. Preparation of novel acrylamide‐based thermoresponsive polymer analogues and their application as thermoresponsive chromatographic matrices
EP0955315A4 (de) Verfahren zur herstellung von polyacrylamiddispersionen

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties