ATE176454T1 - Trennung von nukleinsäurefragmenten - Google Patents

Trennung von nukleinsäurefragmenten

Info

Publication number
ATE176454T1
ATE176454T1 AT94902238T AT94902238T ATE176454T1 AT E176454 T1 ATE176454 T1 AT E176454T1 AT 94902238 T AT94902238 T AT 94902238T AT 94902238 T AT94902238 T AT 94902238T AT E176454 T1 ATE176454 T1 AT E176454T1
Authority
AT
Austria
Prior art keywords
separation
nucleic acid
polymer beads
acid fragments
nucleic acids
Prior art date
Application number
AT94902238T
Other languages
English (en)
Inventor
Gunther Bonn
Christian Huber
Peter Oefner
Original Assignee
Transgenomic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgenomic Inc filed Critical Transgenomic Inc
Application granted granted Critical
Publication of ATE176454T1 publication Critical patent/ATE176454T1/de

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/803Physical recovery methods, e.g. chromatography, grinding

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Saccharide Compounds (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
AT94902238T 1992-11-18 1993-11-17 Trennung von nukleinsäurefragmenten ATE176454T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT0228592A AT398973B (de) 1992-11-18 1992-11-18 Verfahren zur trennung von nukleinsäuren
PCT/US1993/010975 WO1994011305A1 (en) 1992-11-18 1993-11-17 Separation of nucleic acid fragments

Publications (1)

Publication Number Publication Date
ATE176454T1 true ATE176454T1 (de) 1999-02-15

Family

ID=3531515

Family Applications (2)

Application Number Title Priority Date Filing Date
AT0228592A AT398973B (de) 1992-11-18 1992-11-18 Verfahren zur trennung von nukleinsäuren
AT94902238T ATE176454T1 (de) 1992-11-18 1993-11-17 Trennung von nukleinsäurefragmenten

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT0228592A AT398973B (de) 1992-11-18 1992-11-18 Verfahren zur trennung von nukleinsäuren

Country Status (10)

Country Link
US (1) US5585236A (de)
EP (1) EP0669897B1 (de)
JP (1) JP3395906B2 (de)
AT (2) AT398973B (de)
AU (1) AU5667894A (de)
DE (1) DE69323435T2 (de)
DK (1) DK0669897T3 (de)
ES (1) ES2130396T3 (de)
GR (1) GR3029863T3 (de)
WO (1) WO1994011305A1 (de)

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US5795976A (en) * 1995-08-08 1998-08-18 The Board Of Trustees Of The Leland Stanford Junior University Detection of nucleic acid heteroduplex molecules by denaturing high-performance liquid chromatography and methods for comparative sequencing
US6355791B1 (en) * 1995-11-13 2002-03-12 Transgenomic, Inc. Polynucleotide separations on polymeric separation media
US5772889A (en) * 1995-11-13 1998-06-30 Transgenomic, Inc. System and method for performing nucleic acid separations using liquid chromatography
US6066258A (en) 1997-12-05 2000-05-23 Transgenomic, Inc. Polynucleotide separations on polymeric separation media
US6720186B1 (en) * 1998-04-03 2004-04-13 Symyx Technologies, Inc. Method of research for creating and testing novel catalysts, reactions and polymers
JPH10142172A (ja) * 1996-11-06 1998-05-29 Jeol Ltd 分析プロファイル上のマーカ表示方法
US6030527A (en) * 1996-11-13 2000-02-29 Transgenomic, Inc. Apparatus for performing polynucleotide separations using liquid chromatography
US6174441B1 (en) 1996-11-13 2001-01-16 Transgenomic, Inc. Method for performing polynucleotide separations using liquid chromatography
US6475388B1 (en) 1996-11-13 2002-11-05 Transgenomic, Inc. Method and system for RNA analysis by matched ion polynucleotide chromatography
US6372142B1 (en) 1996-11-13 2002-04-16 Transgenomic, Inc. Column for DNA separation by matched ion polynucleotide chromatography
US6482317B2 (en) 1996-11-13 2002-11-19 Transgenomic, Inc. Polynucleotide separations on polymeric separation media
US6103112A (en) * 1996-11-13 2000-08-15 Transgenomic, Inc. MIPC chromatographic apparatus with improved temperature control
US6576133B2 (en) 1996-11-13 2003-06-10 Transgenomic, Inc Method and system for RNA analysis by matched ion polynucleotide chromatography
US5997742A (en) * 1996-11-13 1999-12-07 Transgenomic, Inc. Method for performing polynucleotide separations using liquid chromatography
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US6056877A (en) * 1997-12-05 2000-05-02 Transgenomic, Inc. Non-polar media for polynucleotide separations
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CA2298019A1 (en) * 1997-08-05 1999-02-18 Transgenomic, Inc. Denaturing multi ion polynucleotide chromatography for detecting mutations
US6027898A (en) * 1997-08-18 2000-02-22 Transgenomic, Inc. Chromatographic method for mutation detection using mutation site specifically acting enzymes and chemicals
WO1999009203A1 (en) * 1997-08-18 1999-02-25 Transgenomic, Inc. Chromatographic method for mutation detection using mutation site specifically acting enzymes
CA2309760A1 (en) 1997-10-09 1999-04-22 Robert M. Haefele Modifying double stranded dna to enhance separations by matched ion polynucleotide chromatography
EP1030933A4 (de) 1997-10-14 2003-01-02 Transgenomic Inc Untersuchung von genickter dna mit hilfe von passender ionischer polynukleotidchromatographie
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CN104220597A (zh) 2012-03-22 2014-12-17 和光纯药工业株式会社 使用了嵌入剂的变异基因的识别检测方法
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Also Published As

Publication number Publication date
AU5667894A (en) 1994-06-08
JPH08505700A (ja) 1996-06-18
GR3029863T3 (en) 1999-07-30
ATA228592A (de) 1994-07-15
US5585236A (en) 1996-12-17
ES2130396T3 (es) 1999-07-01
AT398973B (de) 1995-02-27
EP0669897A1 (de) 1995-09-06
DE69323435D1 (de) 1999-03-18
DE69323435T2 (de) 1999-07-01
EP0669897B1 (de) 1999-02-03
WO1994011305A1 (en) 1994-05-26
EP0669897A4 (de) 1996-01-24
DK0669897T3 (da) 1999-09-20
JP3395906B2 (ja) 2003-04-14

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