ATE133494T1 - Verfahren zur verstärkung der chemilumineszenz von enzymgetriggerten 1,2-dioxetanen und seine verwendung - Google Patents

Verfahren zur verstärkung der chemilumineszenz von enzymgetriggerten 1,2-dioxetanen und seine verwendung

Info

Publication number
ATE133494T1
ATE133494T1 AT92916112T AT92916112T ATE133494T1 AT E133494 T1 ATE133494 T1 AT E133494T1 AT 92916112 T AT92916112 T AT 92916112T AT 92916112 T AT92916112 T AT 92916112T AT E133494 T1 ATE133494 T1 AT E133494T1
Authority
AT
Austria
Prior art keywords
triggered
enzyme
chemiluminescence
enhancement
dioxetans
Prior art date
Application number
AT92916112T
Other languages
English (en)
Inventor
Lynne Elizabeth Anderson
Robert Kenneth Kobos
Shay Evan Queen Lane Polsky
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Application granted granted Critical
Publication of ATE133494T1 publication Critical patent/ATE133494T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
AT92916112T 1991-07-31 1992-07-28 Verfahren zur verstärkung der chemilumineszenz von enzymgetriggerten 1,2-dioxetanen und seine verwendung ATE133494T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US73840891A 1991-07-31 1991-07-31

Publications (1)

Publication Number Publication Date
ATE133494T1 true ATE133494T1 (de) 1996-02-15

Family

ID=24967884

Family Applications (1)

Application Number Title Priority Date Filing Date
AT92916112T ATE133494T1 (de) 1991-07-31 1992-07-28 Verfahren zur verstärkung der chemilumineszenz von enzymgetriggerten 1,2-dioxetanen und seine verwendung

Country Status (16)

Country Link
US (1) US5270164A (de)
EP (1) EP0597916B1 (de)
JP (1) JP3299269B2 (de)
AT (1) ATE133494T1 (de)
AU (1) AU2394192A (de)
CA (1) CA2114522A1 (de)
DE (1) DE69207911T2 (de)
DK (1) DK0597916T3 (de)
ES (1) ES2084368T3 (de)
GR (1) GR3019432T3 (de)
HK (1) HK146996A (de)
IE (1) IE74889B1 (de)
IL (1) IL102523A0 (de)
LT (1) LTIP860A (de)
MX (1) MX9204448A (de)
WO (1) WO1993003380A1 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69224651T2 (de) * 1991-11-15 1998-07-23 Cornell Res Foundation Inc Indirekter immunoassay für dioxinähnliche verbindungen
FR2710655B1 (fr) * 1993-09-29 1995-12-01 Inst Nat Rech Securite Procédé pour effectuer un test biologique destiné à identifier et à doser des gènes, des protéines et autres molécules.
US5846753A (en) * 1995-05-30 1998-12-08 The United States Of America As Represented By The Secretary Of The Army Chemiluminescence-based method for rapid and sensitive in-situ detection of organophosphorus compounds and metal ions
US5783381A (en) * 1995-10-19 1998-07-21 Tropix, Inc. Chemiluminescent 1,2-dioxetanes
RU2183327C2 (ru) * 1997-10-09 2002-06-10 Лумитест Лтд. Способ и устройство для измерения пероксидации липидов в биологических жидкостях и суспензиях тканей
US6867005B2 (en) * 2001-10-24 2005-03-15 Beckman Coulter, Inc. Method and apparatus for increasing the dynamic range and accuracy of binding assays
US7166478B2 (en) * 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
US7767404B2 (en) * 2005-08-16 2010-08-03 Chipotle Business Group, Inc. Apparatus and method for single-step immunosorbent assay for single and multiple analytes
US8735142B2 (en) * 2005-08-16 2014-05-27 Chipotle Business Group, Inc. Systems and methods for immunosorbent assays for single and multiple analytes
WO2014173657A1 (de) * 2013-04-24 2014-10-30 Euroimmun Medizinische Labordiagnostika Ag Verfahren zur automatisierten auswertung von inkubierten immunblotstreifen

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8201492A (nl) * 1982-04-07 1983-11-01 Rijksuniversiteit Werkwijze ter bereiding van een op de 4-plaats equatoriaal gesubstitueerde symmetrisch-polycycloethyleenverbinding; op de 4-plaats equatoriaal gesubstitueerde symmetrisch-polycycloethyleenverbinding alsmede een op basis van de verbinding bereid thermochemiluminescerend merkmateriaal of thermochemiluminescerend sondeermateriaal.
US4778767A (en) * 1984-12-17 1988-10-18 Akzo N.V. Solid phase immunoassay using immunoreagents immobilized on inert synthetic resin surfaces
US4857652A (en) * 1986-07-17 1989-08-15 Board Of Governors Of Wayne State University Chemiluminescent 1,2-dioxetane compounds
US4959182A (en) * 1986-07-17 1990-09-25 Board Of Governors Of Wayne State University Method and compositions providing enhanced chemiluminescence from 1,2-dioxetanes
US4956477A (en) * 1987-12-31 1990-09-11 Tropix, Inc. Synthesis of 1,2-dioxetanes
DE3788203T2 (de) * 1986-07-24 1994-03-31 Tropix Inc Verfahren zum nachweis einer verbindung unter verwendung des enzymatisch induzierten zerfalls von dioxethanen.
EP0261719A3 (de) * 1986-09-23 1990-10-10 Akzo N.V. Thermochemischlumineszierende Komplexe von Cyclodextrinen
EP0290269A3 (de) * 1987-05-06 1989-08-09 Cyberfluor Inc. Immunoassayverfahren und Reagenzien und Herstellungsverfahren der letzteren
EP0348494B1 (de) * 1987-12-31 1994-08-10 Tropix, Inc. Dioxetane zur verwendung bei immuntests
US4952707A (en) * 1988-06-30 1990-08-28 Tropix, Inc. Enzymatically-cleavable chemiluminescent fused polycyclic ring-containing 1,2-dioxetanes
US4948975A (en) * 1988-09-08 1990-08-14 The United States Of America As Represented By The Secretary Of The Air Force Quantitative luminescence imaging system

Also Published As

Publication number Publication date
HK146996A (en) 1996-08-09
ES2084368T3 (es) 1996-05-01
EP0597916B1 (de) 1996-01-24
JP3299269B2 (ja) 2002-07-08
IE74889B1 (en) 1997-08-13
DE69207911D1 (de) 1996-03-07
AU2394192A (en) 1993-03-02
DK0597916T3 (da) 1996-02-26
WO1993003380A1 (en) 1993-02-18
JPH06509648A (ja) 1994-10-27
GR3019432T3 (en) 1996-06-30
IL102523A0 (en) 1993-01-14
DE69207911T2 (de) 1996-07-04
US5270164A (en) 1993-12-14
MX9204448A (es) 1993-05-01
IE922462A1 (en) 1993-02-10
LTIP860A (en) 1995-03-27
EP0597916A1 (de) 1994-05-25
CA2114522A1 (en) 1993-02-18

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