LTIP860A - Process for enhancing the chemiluminescence of enzyme-triggered 1,2-dioxetanes and uses thereof - Google Patents

Process for enhancing the chemiluminescence of enzyme-triggered 1,2-dioxetanes and uses thereof

Info

Publication number
LTIP860A
LTIP860A LTIP860A LTIP860A LTIP860A LT IP860 A LTIP860 A LT IP860A LT IP860 A LTIP860 A LT IP860A LT IP860 A LTIP860 A LT IP860A LT IP860 A LTIP860 A LT IP860A
Authority
LT
Lithuania
Prior art keywords
dioxetanes
triggered
enzyme
chemiluminescence
enhancing
Prior art date
Application number
LTIP860A
Inventor
Lynne Elizabeth Anderson
Robert Kenneth Kobos
Shay Evan Polsky
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of LTIP860A publication Critical patent/LTIP860A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

A process is disclosed for the enhancement of the chemiluminescence of enzyme-triggered 1,2-dioxetanes detectable within a sample. The process comprises first providing a solid support having sample disposed thereon and suitably treated with a solution including enzyme-triggered 1,2-dioxetanes. The solid support is next dried and optionally heated either simultaneously with drying or thereafter. The steps of drying and/or heating are conducted either prior to or simultaneously with detection.
LTIP860A 1991-07-31 1993-08-16 Process for enhancing the chemiluminescence of enzyme-triggered 1,2-dioxetanes and uses thereof LTIP860A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US73840891A 1991-07-31 1991-07-31

Publications (1)

Publication Number Publication Date
LTIP860A true LTIP860A (en) 1995-03-27

Family

ID=24967884

Family Applications (1)

Application Number Title Priority Date Filing Date
LTIP860A LTIP860A (en) 1991-07-31 1993-08-16 Process for enhancing the chemiluminescence of enzyme-triggered 1,2-dioxetanes and uses thereof

Country Status (16)

Country Link
US (1) US5270164A (en)
EP (1) EP0597916B1 (en)
JP (1) JP3299269B2 (en)
AT (1) ATE133494T1 (en)
AU (1) AU2394192A (en)
CA (1) CA2114522A1 (en)
DE (1) DE69207911T2 (en)
DK (1) DK0597916T3 (en)
ES (1) ES2084368T3 (en)
GR (1) GR3019432T3 (en)
HK (1) HK146996A (en)
IE (1) IE74889B1 (en)
IL (1) IL102523A0 (en)
LT (1) LTIP860A (en)
MX (1) MX9204448A (en)
WO (1) WO1993003380A1 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3253300B2 (en) * 1991-11-15 2002-02-04 コーネル リサーチ ファウンデイション インコーポレイテッド Indirect immunoassay for dioxin analogs
FR2710655B1 (en) * 1993-09-29 1995-12-01 Inst Nat Rech Securite Method for carrying out a biological test intended to identify and assay genes, proteins and other molecules.
US5846753A (en) * 1995-05-30 1998-12-08 The United States Of America As Represented By The Secretary Of The Army Chemiluminescence-based method for rapid and sensitive in-situ detection of organophosphorus compounds and metal ions
US5783381A (en) * 1995-10-19 1998-07-21 Tropix, Inc. Chemiluminescent 1,2-dioxetanes
IL135233A (en) * 1997-10-09 2003-03-12 Lumitest Ltd Method and apparatus for measuring lipid peroxidation in biological fluids and suspensions of tissues
US6867005B2 (en) * 2001-10-24 2005-03-15 Beckman Coulter, Inc. Method and apparatus for increasing the dynamic range and accuracy of binding assays
US7166478B2 (en) * 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
US8735142B2 (en) * 2005-08-16 2014-05-27 Chipotle Business Group, Inc. Systems and methods for immunosorbent assays for single and multiple analytes
US7767404B2 (en) * 2005-08-16 2010-08-03 Chipotle Business Group, Inc. Apparatus and method for single-step immunosorbent assay for single and multiple analytes
US10914737B2 (en) * 2013-04-24 2021-02-09 Euroimmun Medizinische Labordiagnostika Ag Method for automated evaluation of incubated immunoblot strips

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8201492A (en) * 1982-04-07 1983-11-01 Rijksuniversiteit PROCESS FOR PREPARING A 4-PLACE EQUATORALLY SUBSTITUTED SYMMETRICAL-POLYCYCLETHYLENE COMPOUND; SYMMETRICAL-POLYCYCLETHYLENE COMPOUND SUBSTITUTED IN THE 4-PLACE EQUATORALLY COMPOSITION AND A THERMOCHEMILUMINUM SCREENING PROCESS MATERIAL PREPARED ON THE BASIS OF THE COMPOUND.
US4778767A (en) * 1984-12-17 1988-10-18 Akzo N.V. Solid phase immunoassay using immunoreagents immobilized on inert synthetic resin surfaces
US4959182A (en) * 1986-07-17 1990-09-25 Board Of Governors Of Wayne State University Method and compositions providing enhanced chemiluminescence from 1,2-dioxetanes
US4857652A (en) * 1986-07-17 1989-08-15 Board Of Governors Of Wayne State University Chemiluminescent 1,2-dioxetane compounds
US4956477A (en) * 1987-12-31 1990-09-11 Tropix, Inc. Synthesis of 1,2-dioxetanes
DE3788203T2 (en) * 1986-07-24 1994-03-31 Tropix Inc METHOD FOR DETECTING A CONNECTION USING THE ENZYMATICALLY INDUCED DECOMPOSITION OF DIOXETHANES.
EP0261719A3 (en) * 1986-09-23 1990-10-10 Akzo N.V. Thermochemiluminescent cyclodextrin complexes
US5089423A (en) * 1987-05-06 1992-02-18 Cyberfluor Inc. Immunoassay methods and reagents and methods for producing the latter
EP0348494B1 (en) * 1987-12-31 1994-08-10 Tropix, Inc. Dioxetanes for use in assays
US4952707A (en) * 1988-06-30 1990-08-28 Tropix, Inc. Enzymatically-cleavable chemiluminescent fused polycyclic ring-containing 1,2-dioxetanes
US4948975A (en) * 1988-09-08 1990-08-14 The United States Of America As Represented By The Secretary Of The Air Force Quantitative luminescence imaging system

Also Published As

Publication number Publication date
GR3019432T3 (en) 1996-06-30
IE74889B1 (en) 1997-08-13
ATE133494T1 (en) 1996-02-15
CA2114522A1 (en) 1993-02-18
US5270164A (en) 1993-12-14
JPH06509648A (en) 1994-10-27
EP0597916A1 (en) 1994-05-25
IL102523A0 (en) 1993-01-14
DE69207911T2 (en) 1996-07-04
WO1993003380A1 (en) 1993-02-18
MX9204448A (en) 1993-05-01
EP0597916B1 (en) 1996-01-24
ES2084368T3 (en) 1996-05-01
HK146996A (en) 1996-08-09
JP3299269B2 (en) 2002-07-08
AU2394192A (en) 1993-03-02
DE69207911D1 (en) 1996-03-07
DK0597916T3 (en) 1996-02-26
IE922462A1 (en) 1993-02-10

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Legal Events

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FD9A Withdrawn applications

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