ATE107356T1 - Verfahren zur herstellung eines proteins. - Google Patents
Verfahren zur herstellung eines proteins.Info
- Publication number
- ATE107356T1 ATE107356T1 AT87110601T AT87110601T ATE107356T1 AT E107356 T1 ATE107356 T1 AT E107356T1 AT 87110601 T AT87110601 T AT 87110601T AT 87110601 T AT87110601 T AT 87110601T AT E107356 T1 ATE107356 T1 AT E107356T1
- Authority
- AT
- Austria
- Prior art keywords
- protein
- aqueous solution
- alkaline aqueous
- insoluble form
- soluble native
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
- C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB838308234A GB8308234D0 (en) | 1983-03-25 | 1983-03-25 | Recovery of products produced by host organisms |
PCT/GB1983/000152 WO1983004418A1 (en) | 1982-06-07 | 1983-06-07 | A process for the preparation of chymosin |
GB838327345A GB8327345D0 (en) | 1983-06-07 | 1983-10-12 | Production of protein |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE107356T1 true ATE107356T1 (de) | 1994-07-15 |
Family
ID=27261901
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT87110601T ATE107356T1 (de) | 1983-03-25 | 1984-03-23 | Verfahren zur herstellung eines proteins. |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0122080B1 (de) |
JP (1) | JPH06102034B2 (de) |
AT (1) | ATE107356T1 (de) |
DE (1) | DE122080T1 (de) |
GB (1) | GB2138004B (de) |
WO (1) | WO1984003711A1 (de) |
Families Citing this family (37)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4512922A (en) * | 1982-12-22 | 1985-04-23 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
US4511502A (en) * | 1982-12-22 | 1985-04-16 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
US4599197A (en) * | 1982-12-22 | 1986-07-08 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
US4518526A (en) * | 1982-12-22 | 1985-05-21 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
US4620948A (en) * | 1982-12-22 | 1986-11-04 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
US5340926A (en) * | 1983-03-25 | 1994-08-23 | Celltech, Limited | Process for the recovery of recombinantly produced protein from insoluble aggregate |
BG49718A3 (en) * | 1983-07-15 | 1992-01-15 | Bio Technology General Corp | Method for preparing of polypeptid with superoxiddismutasne activitty |
US4721673A (en) * | 1983-09-01 | 1988-01-26 | Genex Corporation | Recovery and activation process for microbially produced calf prochymosin |
US4530787A (en) * | 1984-03-28 | 1985-07-23 | Cetus Corporation | Controlled oxidation of microbially produced cysteine-containing proteins |
US5871956A (en) * | 1984-12-06 | 1999-02-16 | Amgen Inc. | Recombinant methods for production of serine inhibitors and DNA sequences useful for same |
US6291662B1 (en) | 1984-12-05 | 2001-09-18 | Amgen Inc. | Recombinant methods for production of serine protease inhibitors and DNA sequences |
US5900400A (en) * | 1984-12-06 | 1999-05-04 | Amgen Inc. | Serine protease inhibitor analogs |
US6132990A (en) * | 1984-12-06 | 2000-10-17 | Amgen Boulder Inc. | Recombinant methods for production of serine protease inhibitors and DNA sequences useful for same |
HU204563B (en) * | 1984-12-06 | 1992-01-28 | Synergen Biolog Inc | Process for producing recombinant serine-protease inhibitors and dns sequencyes for them |
JPH088870B2 (ja) * | 1985-02-05 | 1996-01-31 | サイナ−ゲン バイオロジカルズ,インコ−ポレ−テツド | メタロプロテイナ−ゼ阻害剤の配列をもつ組換ベクタ−系及びメタロプロテイナ−ゼ阻害剤製造のための組換dna |
ES8800957A1 (es) * | 1985-02-22 | 1987-12-01 | Monsanto Co | Un metodo para la solubilizacion y renaturalizacion de proteina somatotropina |
GB8508340D0 (en) * | 1985-03-29 | 1985-05-09 | Creighton T E | Production of protein |
US4873312A (en) * | 1985-04-25 | 1989-10-10 | Amgen | Method for purifying interferon and composition of matter produced thereby |
US5453363A (en) * | 1985-10-23 | 1995-09-26 | Boehringer Mannheim Gmbh | Process for the activation of t-PA or Ing after genetic expression in prokaryotes |
DE3537708A1 (de) * | 1985-10-23 | 1987-04-23 | Boehringer Mannheim Gmbh | Verfahren zur aktivierung von t-pa nach expression in prokaryonten |
US4766205A (en) * | 1985-11-13 | 1988-08-23 | Beatrice Companies, Inc. | Method for isolation of recombinant polypeptides in biologically active forms |
DE3611817A1 (de) * | 1986-04-08 | 1987-10-15 | Boehringer Mannheim Gmbh | Verfahren zur renaturierung von proteinen |
US4705848A (en) * | 1986-06-02 | 1987-11-10 | International Minerals & Chemical Corp. | Isolation of bioactive, monomeric growth hormone |
JPH01222780A (ja) * | 1988-03-02 | 1989-09-06 | Tosoh Corp | ウロキナーゼ前駆体様蛋白質のリフォールディング方法 |
US5006472A (en) * | 1988-06-03 | 1991-04-09 | Miles Inc. | Enzymatic purification process |
US5637566A (en) * | 1988-08-24 | 1997-06-10 | Southern Cross Biotech Pty. Ltd. | Method of improving carcass quality by administering growth hormone |
US5064943A (en) * | 1988-12-16 | 1991-11-12 | American Cyanamid Company | Method for solubilization and naturation of somatotropin |
DE4039415A1 (de) | 1990-02-03 | 1991-08-08 | Boehringer Mannheim Gmbh | Verfahren zur herstellung rekombinanter proteine ohne n-terminalen methioninrest |
WO1992014832A1 (en) * | 1991-02-26 | 1992-09-03 | Ajinomoto Co., Inc. | Processes for purifying human bcdf |
US6869925B1 (en) * | 1992-09-09 | 2005-03-22 | Amgen Inc. | Inhibition of retrovirus infection |
NZ261571A (en) * | 1993-02-04 | 1997-03-24 | Denzyme Aps | Producing correctly folded proteins using multiple denaturation and renaturation cycles |
US6017880A (en) * | 1994-03-09 | 2000-01-25 | Amgen Inc. | Inhibition of retrovirus infection |
ATE323759T1 (de) * | 1999-01-25 | 2006-05-15 | Novozymes As | Rückgewinnung eines proteins bei hohem ph-wert |
SG176963A1 (en) | 2009-06-22 | 2012-02-28 | Amgen Inc | Refolding proteins using a chemically controlled redox state |
MX2011013417A (es) | 2009-06-25 | 2012-03-29 | Amgen Inc | Procesos de purificacion por captura para proteinas expresadas en un sistema no mamifero. |
CN102892780B (zh) | 2010-03-17 | 2015-07-29 | 通益制药有限公司 | 获得生物活性的重组人g-csf的方法 |
JP6484064B2 (ja) * | 2015-02-25 | 2019-03-13 | ヤマシンフィルタ株式会社 | フィルタエレメント |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1258063A (de) * | 1968-08-23 | 1971-12-22 | ||
IE53517B1 (en) * | 1981-06-17 | 1988-12-07 | Celltech Ltd | Process for the production of a polypeptide |
IN158842B (de) * | 1981-11-13 | 1987-01-31 | Blue Circle Ind Plc | |
AU553017B2 (en) * | 1982-06-07 | 1986-06-26 | Chr. Hansen A/S | A process for the preparation of chymosin |
-
1984
- 1984-03-23 JP JP50161184A patent/JPH06102034B2/ja not_active Expired - Lifetime
- 1984-03-23 GB GB08407570A patent/GB2138004B/en not_active Expired
- 1984-03-23 EP EP84301995A patent/EP0122080B1/de not_active Expired
- 1984-03-23 WO PCT/GB1984/000093 patent/WO1984003711A1/en unknown
- 1984-03-23 DE DE198484301995T patent/DE122080T1/de active Pending
- 1984-03-23 AT AT87110601T patent/ATE107356T1/de not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
GB2138004B (en) | 1987-05-13 |
GB8407570D0 (en) | 1984-05-02 |
EP0122080A1 (de) | 1984-10-17 |
DE122080T1 (de) | 1985-05-23 |
JPH06102034B2 (ja) | 1994-12-14 |
JPS60500893A (ja) | 1985-06-20 |
EP0122080B1 (de) | 1989-09-13 |
WO1984003711A1 (en) | 1984-09-27 |
GB2138004A (en) | 1984-10-17 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
UEP | Publication of translation of european patent specification | ||
RZN | Patent revoked |