ATE107356T1 - Verfahren zur herstellung eines proteins. - Google Patents

Verfahren zur herstellung eines proteins.

Info

Publication number
ATE107356T1
ATE107356T1 AT87110601T AT87110601T ATE107356T1 AT E107356 T1 ATE107356 T1 AT E107356T1 AT 87110601 T AT87110601 T AT 87110601T AT 87110601 T AT87110601 T AT 87110601T AT E107356 T1 ATE107356 T1 AT E107356T1
Authority
AT
Austria
Prior art keywords
protein
aqueous solution
alkaline aqueous
insoluble form
soluble native
Prior art date
Application number
AT87110601T
Other languages
English (en)
Inventor
Peter Anthony Lowe
Fiona Angela Olinda Marston
Sarojani Angal
Joyce Ann Schoemaker
Original Assignee
Celltech Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27261901&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE107356(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from GB838308234A external-priority patent/GB8308234D0/en
Priority claimed from PCT/GB1983/000152 external-priority patent/WO1983004418A1/en
Priority claimed from GB838327345A external-priority patent/GB8327345D0/en
Application filed by Celltech Ltd filed Critical Celltech Ltd
Application granted granted Critical
Publication of ATE107356T1 publication Critical patent/ATE107356T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT87110601T 1983-03-25 1984-03-23 Verfahren zur herstellung eines proteins. ATE107356T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB838308234A GB8308234D0 (en) 1983-03-25 1983-03-25 Recovery of products produced by host organisms
PCT/GB1983/000152 WO1983004418A1 (en) 1982-06-07 1983-06-07 A process for the preparation of chymosin
GB838327345A GB8327345D0 (en) 1983-06-07 1983-10-12 Production of protein

Publications (1)

Publication Number Publication Date
ATE107356T1 true ATE107356T1 (de) 1994-07-15

Family

ID=27261901

Family Applications (1)

Application Number Title Priority Date Filing Date
AT87110601T ATE107356T1 (de) 1983-03-25 1984-03-23 Verfahren zur herstellung eines proteins.

Country Status (6)

Country Link
EP (1) EP0122080B1 (de)
JP (1) JPH06102034B2 (de)
AT (1) ATE107356T1 (de)
DE (1) DE122080T1 (de)
GB (1) GB2138004B (de)
WO (1) WO1984003711A1 (de)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4518526A (en) * 1982-12-22 1985-05-21 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511502A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4599197A (en) * 1982-12-22 1986-07-08 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4620948A (en) * 1982-12-22 1986-11-04 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4512922A (en) * 1982-12-22 1985-04-23 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US5340926A (en) * 1983-03-25 1994-08-23 Celltech, Limited Process for the recovery of recombinantly produced protein from insoluble aggregate
BG49718A3 (en) * 1983-07-15 1992-01-15 Bio- Technology General Corp Method for preparing of polypeptid with superoxiddismutasne activitty
US4721673A (en) * 1983-09-01 1988-01-26 Genex Corporation Recovery and activation process for microbially produced calf prochymosin
US4530787A (en) * 1984-03-28 1985-07-23 Cetus Corporation Controlled oxidation of microbially produced cysteine-containing proteins
US5871956A (en) * 1984-12-06 1999-02-16 Amgen Inc. Recombinant methods for production of serine inhibitors and DNA sequences useful for same
US6291662B1 (en) 1984-12-05 2001-09-18 Amgen Inc. Recombinant methods for production of serine protease inhibitors and DNA sequences
ATE108205T1 (de) * 1984-12-06 1994-07-15 Synergen Biolog Inc Rekombinantverfahren zur herstellung von serinproteaseinhibitoren, sowie dns-sequenzen dazu.
US5900400A (en) * 1984-12-06 1999-05-04 Amgen Inc. Serine protease inhibitor analogs
US6132990A (en) * 1984-12-06 2000-10-17 Amgen Boulder Inc. Recombinant methods for production of serine protease inhibitors and DNA sequences useful for same
WO1986004608A1 (en) * 1985-02-05 1986-08-14 Synergen Biologicals, Inc. Metalloproteinase inhibitor sequence recombinant vector system for using same and recombinant-dna method for the manufacture of same
ES8800957A1 (es) * 1985-02-22 1987-12-01 Monsanto Co Un metodo para la solubilizacion y renaturalizacion de proteina somatotropina
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
US4873312A (en) * 1985-04-25 1989-10-10 Amgen Method for purifying interferon and composition of matter produced thereby
US5453363A (en) * 1985-10-23 1995-09-26 Boehringer Mannheim Gmbh Process for the activation of t-PA or Ing after genetic expression in prokaryotes
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
DE3611817A1 (de) * 1986-04-08 1987-10-15 Boehringer Mannheim Gmbh Verfahren zur renaturierung von proteinen
US4705848A (en) * 1986-06-02 1987-11-10 International Minerals & Chemical Corp. Isolation of bioactive, monomeric growth hormone
JPH01222780A (ja) * 1988-03-02 1989-09-06 Tosoh Corp ウロキナーゼ前駆体様蛋白質のリフォールディング方法
US5006472A (en) * 1988-06-03 1991-04-09 Miles Inc. Enzymatic purification process
US5637566A (en) * 1988-08-24 1997-06-10 Southern Cross Biotech Pty. Ltd. Method of improving carcass quality by administering growth hormone
US5064943A (en) * 1988-12-16 1991-11-12 American Cyanamid Company Method for solubilization and naturation of somatotropin
DE4039415A1 (de) 1990-02-03 1991-08-08 Boehringer Mannheim Gmbh Verfahren zur herstellung rekombinanter proteine ohne n-terminalen methioninrest
JP3200850B2 (ja) * 1991-02-26 2001-08-20 味の素株式会社 ヒトｂｃｄｆの精製法
US6869925B1 (en) * 1992-09-09 2005-03-22 Amgen Inc. Inhibition of retrovirus infection
NZ261571A (en) * 1993-02-04 1997-03-24 Denzyme Aps Producing correctly folded proteins using multiple denaturation and renaturation cycles
US6017880A (en) * 1994-03-09 2000-01-25 Amgen Inc. Inhibition of retrovirus infection
JP2002534975A (ja) 1999-01-25 2002-10-22 ノボザイムス アクティーゼルスカブ 高ｐＨにおいてのタンパク質の回収
EP3366692A1 (de) 2009-06-22 2018-08-29 Amgen, Inc Proteinneufaltung mit einem chemisch gesteuerten redoxzustand
EP2445924B2 (de) 2009-06-25 2023-12-13 Amgen Inc. Vefahren zur aufreinigung von proteinen aus prokaryotischen zellen
WO2011113601A1 (en) 2010-03-17 2011-09-22 Biogenerix Ag Method for obtaining biologically active recombinant human g-csf
JP6484064B2 (ja) * 2015-02-25 2019-03-13 ヤマシンフィルタ株式会社 フィルタエレメント

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1258063A (de) * 1968-08-23 1971-12-22
IE53517B1 (en) * 1981-06-17 1988-12-07 Celltech Ltd Process for the production of a polypeptide
IN158842B (de) * 1981-11-13 1987-01-31 Blue Circle Ind Plc
EP0112849B1 (de) * 1982-06-07 1987-09-16 Celltech Limited Verfahren zur herstellung von chymosin

Also Published As

Publication number Publication date
WO1984003711A1 (en) 1984-09-27
DE122080T1 (de) 1985-05-23
JPS60500893A (ja) 1985-06-20
EP0122080A1 (de) 1984-10-17
JPH06102034B2 (ja) 1994-12-14
GB2138004A (en) 1984-10-17
GB8407570D0 (en) 1984-05-02
GB2138004B (en) 1987-05-13
EP0122080B1 (de) 1989-09-13

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Legal Events

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UEP Publication of translation of european patent specification
RZN Patent revoked