AT337891B - Verfahren zur herstellung von proteinpraparaten - Google Patents
Verfahren zur herstellung von proteinpraparatenInfo
- Publication number
- AT337891B AT337891B AT16375A AT16375A AT337891B AT 337891 B AT337891 B AT 337891B AT 16375 A AT16375 A AT 16375A AT 16375 A AT16375 A AT 16375A AT 337891 B AT337891 B AT 337891B
- Authority
- AT
- Austria
- Prior art keywords
- sep
- carrier
- production
- protein preparations
- starting material
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 7
- 238000004519 manufacturing process Methods 0.000 title claims description 6
- 108090000623 proteins and genes Proteins 0.000 title description 3
- 102000004169 proteins and genes Human genes 0.000 title description 3
- 238000002360 preparation method Methods 0.000 title description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 150000004984 aromatic diamines Chemical class 0.000 claims description 2
- 239000000741 silica gel Substances 0.000 claims description 2
- 229910002027 silica gel Inorganic materials 0.000 claims description 2
- 239000007858 starting material Substances 0.000 claims 4
- WZCQRUWWHSTZEM-UHFFFAOYSA-N 1,3-phenylenediamine Chemical compound NC1=CC=CC(N)=C1 WZCQRUWWHSTZEM-UHFFFAOYSA-N 0.000 claims 1
- YBRVSVVVWCFQMG-UHFFFAOYSA-N 4,4'-diaminodiphenylmethane Chemical compound C1=CC(N)=CC=C1CC1=CC=C(N)C=C1 YBRVSVVVWCFQMG-UHFFFAOYSA-N 0.000 claims 1
- KOGDFDWINXIWHI-OWOJBTEDSA-N 4-[(e)-2-(4-aminophenyl)ethenyl]aniline Chemical compound C1=CC(N)=CC=C1\C=C\C1=CC=C(N)C=C1 KOGDFDWINXIWHI-OWOJBTEDSA-N 0.000 claims 1
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 claims 1
- 239000004952 Polyamide Substances 0.000 claims 1
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 claims 1
- 239000012876 carrier material Substances 0.000 claims 1
- 239000001913 cellulose Substances 0.000 claims 1
- 229920002678 cellulose Polymers 0.000 claims 1
- 229920002647 polyamide Polymers 0.000 claims 1
- 239000005373 porous glass Substances 0.000 claims 1
- 239000000843 powder Substances 0.000 claims 1
- 239000005018 casein Substances 0.000 description 4
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 4
- 235000021240 caseins Nutrition 0.000 description 4
- 108010004032 Bromelains Proteins 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000270 Ficain Proteins 0.000 description 2
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 102000004142 Trypsin Human genes 0.000 description 2
- 108090000631 Trypsin Proteins 0.000 description 2
- 108010047754 beta-Glucosidase Proteins 0.000 description 2
- 102000006995 beta-Glucosidase Human genes 0.000 description 2
- POTUGHMKJGOKRI-UHFFFAOYSA-N ficin Chemical compound FI=CI=N POTUGHMKJGOKRI-UHFFFAOYSA-N 0.000 description 2
- 235000019836 ficin Nutrition 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 239000012588 trypsin Substances 0.000 description 2
- 239000001100 (2S)-5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)chroman-4-one Substances 0.000 description 1
- 239000001606 7-[(2S,3R,4S,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-2-yl]oxy-5-hydroxy-2-(4-hydroxyphenyl)chroman-4-one Substances 0.000 description 1
- QUQPHWDTPGMPEX-UHFFFAOYSA-N Hesperidine Natural products C1=C(O)C(OC)=CC=C1C1OC2=CC(OC3C(C(O)C(O)C(COC4C(C(O)C(O)C(C)O4)O)O3)O)=CC(O)=C2C(=O)C1 QUQPHWDTPGMPEX-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical group [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- QUQPHWDTPGMPEX-UTWYECKDSA-N aurantiamarin Natural products COc1ccc(cc1O)[C@H]1CC(=O)c2c(O)cc(O[C@@H]3O[C@H](CO[C@@H]4O[C@@H](C)[C@H](O)[C@@H](O)[C@H]4O)[C@@H](O)[C@H](O)[C@H]3O)cc2O1 QUQPHWDTPGMPEX-UTWYECKDSA-N 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- APSNPMVGBGZYAJ-GLOOOPAXSA-N clematine Natural products COc1cc(ccc1O)[C@@H]2CC(=O)c3c(O)cc(O[C@@H]4O[C@H](CO[C@H]5O[C@@H](C)[C@H](O)[C@@H](O)[C@H]5O)[C@@H](O)[C@H](O)[C@H]4O)cc3O2 APSNPMVGBGZYAJ-GLOOOPAXSA-N 0.000 description 1
- QGGZBXOADPVUPN-UHFFFAOYSA-N dihydrochalcone Chemical compound C=1C=CC=CC=1C(=O)CCC1=CC=CC=C1 QGGZBXOADPVUPN-UHFFFAOYSA-N 0.000 description 1
- PXLWOFBAEVGBOA-UHFFFAOYSA-N dihydrochalcone Natural products OC1C(O)C(O)C(CO)OC1C1=C(O)C=CC(C(=O)CC(O)C=2C=CC(O)=CC=2)=C1O PXLWOFBAEVGBOA-UHFFFAOYSA-N 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- VUYDGVRIQRPHFX-UHFFFAOYSA-N hesperidin Natural products COc1cc(ccc1O)C2CC(=O)c3c(O)cc(OC4OC(COC5OC(O)C(O)C(O)C5O)C(O)C(O)C4O)cc3O2 VUYDGVRIQRPHFX-UHFFFAOYSA-N 0.000 description 1
- QUQPHWDTPGMPEX-QJBIFVCTSA-N hesperidin Chemical compound C1=C(O)C(OC)=CC=C1[C@H]1OC2=CC(O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO[C@H]4[C@@H]([C@H](O)[C@@H](O)[C@H](C)O4)O)O3)O)=CC(O)=C2C(=O)C1 QUQPHWDTPGMPEX-QJBIFVCTSA-N 0.000 description 1
- 229940025878 hesperidin Drugs 0.000 description 1
- 108010030923 hesperidinase Proteins 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- RKDYKIHMFYAPMZ-UHFFFAOYSA-N n-[5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]benzamide Chemical compound C=1C=C([N+]([O-])=O)C=CC=1NC(=O)C(CCCN=C(N)N)NC(=O)C1=CC=CC=C1 RKDYKIHMFYAPMZ-UHFFFAOYSA-N 0.000 description 1
- DFPMSGMNTNDNHN-ZPHOTFPESA-N naringin Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](OC=2C=C3O[C@@H](CC(=O)C3=C(O)C=2)C=2C=CC(O)=CC=2)O[C@H](CO)[C@@H](O)[C@@H]1O DFPMSGMNTNDNHN-ZPHOTFPESA-N 0.000 description 1
- 229940052490 naringin Drugs 0.000 description 1
- 229930019673 naringin Natural products 0.000 description 1
- 108010001078 naringinase Proteins 0.000 description 1
- ARGKVCXINMKCAZ-UHFFFAOYSA-N neohesperidine Natural products C1=C(O)C(OC)=CC=C1C1OC2=CC(OC3C(C(O)C(O)C(CO)O3)OC3C(C(O)C(O)C(C)O3)O)=CC(O)=C2C(=O)C1 ARGKVCXINMKCAZ-UHFFFAOYSA-N 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229920003176 water-insoluble polymer Polymers 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Description
<Desc/Clms Page number 1> EMI1.1 <Desc/Clms Page number 2> Tabelle EMI2.1 EMI2.2 <tb> <tb> Trägerbasis: <SEP> poröses <SEP> Glas <tb> Eingesetzte <SEP> Aktivität <tb> Enzymmenge <SEP> pro <SEP> g <SEP> Träger <SEP> Substrat <tb> Enzym <tb> [mg <SEP> Protein] <SEP> [Einheiten] <SEP> Einheiten <tb> N <SEP> aringinas <SEP> e <SEP> 1 <SEP> 3, <SEP> 4 <SEP> 1, <SEP> 9 <SEP> Naringin <tb> 2, <SEP> 3 <SEP> 8 <SEP> 2, <SEP> 4 <SEP> <tb> Amyloglucosidase <SEP> 4 <SEP> 192 <SEP> 50 <SEP> Stärke <tb> 8 <SEP> 384 <SEP> 90 <tb> Hesperidinase <SEP> 2 <SEP> 10,4 <SEP> 4,6 <SEP> Hesperidin-dihydrochalcon <tb> Ficin <SEP> 2 <SEP> 1, <SEP> 2 <SEP> 0, <SEP> 38 <SEP> Casein <tb> Trypsin <SEP> 2,5 <SEP> 5,5 <SEP> 1,9 <SEP> N-Benzoly-dl-argini-4-nitro-anilid <tb> Bromelin <SEP> 2 <SEP> 1, <SEP> 1 <SEP> 0, <SEP> 32 <SEP> Casein <tb> ss-Glucosidase <SEP> 2 <SEP> 8,8 <SEP> 2, 3 <SEP> N-Nitrophenyl-glycopyranosid <tb> Trägerbasis <SEP> : <SEP> Silicagel <SEP> <tb> Naringinase <SEP> 3 <SEP> 10 <SEP> 1,8 <SEP> Naringin- <SEP> <tb> Amyloglucosidase <SEP> 2 <SEP> 90 <SEP> 9, <SEP> 4 <SEP> Stärke <tb> Trypsin <SEP> 2, <SEP> 5 <SEP> 5, <SEP> 5 <SEP> 2, <SEP> 1 <SEP> N-Benzoyl-dl-arginin-4-nitro-anilid <SEP> <tb> Ficin <SEP> 2 <SEP> 1, <SEP> 2 <SEP> 0, <SEP> 44 <SEP> Casein <tb> Bromelin <SEP> 2 <SEP> 1, <SEP> 1 <SEP> 0, <SEP> 35 <SEP> Casein <tb> ss-Glucosidase <SEP> 2 <SEP> 8, <SEP> 8 <SEP> 1, <SEP> 76 <SEP> N-Nitrophenyl-glycopyranosid <SEP> <tb> PATENTANSPRÜCHE : 1. Verfahren zur Herstellung von Proteinpräparaten, dadurch gekennzeichnet, dass man einen Träger, der durch Behandlung eines wasserunlöslichen Polymeren oder eines wasserunlöslichen anorgani- schen, reaktive Sauerstoffunktionen enthaltenden Materials mit einem aromatischen Diamin und Diazotierung des Reaktionsproduktes erhältlich ist, mit einer Proteinlösung behandelt.
Claims (1)
- 2. Verfahren nach Anspruch 1, dadurch gekennzeichnet, dass als Ausgangsmaterial für die Herstellung des Trägers ein Silikat verwendet wird.3. Verfahren nach Anspruch 2, dadurch gekennzeichnet, dass als Ausgangsmaterial für die Herstellung des Trägers Silicagel oder poröses Glaspulver verwendet wird.4. Verfahren nach Anspruch 1, dadurch gekennzeichnet, dass als Ausgangsmaterial für die Herstellung des Trägers ein Polyamid verwendet wird.5. Verfahren nach Anspruch 1, dadurch gekennzeichnet, dass als Ausgangsmaterial für die Herstellung des Trägers Cellulose verwendet wird.6. Verfahren nach einem der Ansprüche Ibis 5, dadurch gekennzeichnet, dass manzur Be- handlung des Trägermaterials als aromatisches Diamin 1,3-Phenylendiamin, 4,4'-Diamino-diphenylmethan, Benzidin oder 4, 4' -Diaminostilben verwendet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AT16375A AT337891B (de) | 1972-07-12 | 1975-01-10 | Verfahren zur herstellung von proteinpraparaten |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH1042272A CH579533A5 (de) | 1972-07-12 | 1972-07-12 | |
| AT420673A AT326813B (de) | 1972-07-12 | 1973-05-14 | Verfahren zur herstellung eines tragermaterials für proteine |
| AT16375A AT337891B (de) | 1972-07-12 | 1975-01-10 | Verfahren zur herstellung von proteinpraparaten |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ATA16375A ATA16375A (de) | 1976-11-15 |
| AT337891B true AT337891B (de) | 1977-07-25 |
Family
ID=27145923
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT16375A AT337891B (de) | 1972-07-12 | 1975-01-10 | Verfahren zur herstellung von proteinpraparaten |
Country Status (1)
| Country | Link |
|---|---|
| AT (1) | AT337891B (de) |
-
1975
- 1975-01-10 AT AT16375A patent/AT337891B/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| ATA16375A (de) | 1976-11-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| ELJ | Ceased due to non-payment of the annual fee | ||
| REN | Ceased due to non-payment of the annual fee |