AT10665U1 - Yeast-containing, dry rock compound, preparation for its use and method for its production - Google Patents

Description

Condition AT10 665 U1 2009-08-15

Description: The invention relates to a dry attractant combination for fruit flies, a preparation for using the dry attractant combination, a trap for fruit flies and a process for producing a dry attractant combination. The invention relates to the field of attractants for insects, in particular for Drosophilidae flies and Drosophila flies.

The family of fruit flies or Drosophilidae, also called fruit, fruit or vinegar flies, belongs within the order of the two-winged to subordinate the flies. They are small, about 2 mm large flies that occur almost everywhere, both in forests and in residential areas.

The term fruit fly is due to their preference for rotting fruits. The term Drosophila is derived from the behavior of these animals, since they are mostly flying in the morning and in the evening flying.

Worldwide, about 3,000 species are known that live in different ecological areas. The family Drosophila or Drosophilidae includes the subfamily Dro-Sophilinae. A genus of Drosophilinae is Drosophila. This genus has about 1,500 different species, of which Drosophila melanogaster is the best known. Drosophila melano-gaster is also called black-bellied fruit fly and is one of the best-studied organisms in the world. Other common species include Drosophila immigrans or Drosophila hydei.

Baptism is a problem as pests in many areas of the food industry. Especially in the warm season, there is a mass occurrence of these insects. The feeding activity of the fly larvae and the transmission of harmful microorganisms significantly accelerate spoilage of food. Due to their enormous propagation potential, these flies can occur in masses within a very short time. Fruit flies cause considerable damage and affect the well-being of humans. Fruit flies are among the most important pests in the food industry. Some species are typical culture followers, who prefer to stay in and around human habitations to attack mainly fruits and vegetables. Until a few years ago, these species were not considered pests but only as harmless " pests " considered. However, since the EU Food Hygiene Regulation 2004 (EC) No. 852/2004, fruit flies are clearly classified as pests and must be combated accordingly.

It is known that fruit flies are particularly attracted to mature and rotting plant material in order to use this for their oviposition and their breeding site. As a guide to these breeding grounds, flies are used by flies, which rise from organic material.

Numerous methods and attractants are known from the prior art to attract or destroy Drosophila flies. In particular, in the food industry and in supermarkets there is a need to make the traps or attractant combinations such that they exert no negative and harmful influences on the food itself. In most cases this is achieved by traps containing liquid attractants.

For example, from US Pat. No. 6,543,181 B1 a trap with a liquid attractant combination comprising short-chain carboxylic acids, alcohols and nitrogen-containing substituents is known.

It is also known to use fruit pulp or yeast as an attractant for Drosophila. It is also known for some insects, such as cockroaches, to use aggregation pheromones as an attractant.

A disadvantage of the use of fresh fruit pulp, however, is the fact that fresh fruit pulp is perishable and the trap on the one hand quickly loses its effect and on the other hand, a storage of such attractant combination containing fresh fruit pulp, is not possible.

Also, most known from the prior art attractants are highly volatile at room temperature and thus may not be added until shortly before the activation of the trap so that they do not evaporate prematurely. A storage of such liquid attractant combinations is therefore not well possible.

The object of the invention is therefore to provide an attractant combination for Drosophilidae or Taufliegen, especially the genus Drosophila, which is particularly effective and at the same time easy to handle and storable.

This object is solved by the features of claim 1.

By the features of claim 1, a dry attractant combination is described, which contains fruit pulp and yeast in dry form and a Drosophilidae or Drosophila specific aggregation pheromone, which is immobilized with silica.

In this way, a long storable and stable and not spoilage spoilage combination is given, which is easy to handle due to their dry consistency, fill and store. The normally volatile attractants, in this case the aggregation pheromone, is immobilized on silica, whereby no evaporation of the pheromone can occur. In this way, it is also possible to ensure a precisely defined concentration of the individual attractants which is constant over longer periods of time. The attractant combination neither changes its effectiveness, nor do the concentrations of the individual attractants shift each other during the storage period. Another advantage of such dry attractant combinations is, inter alia, that it, unlike liquid attractant combinations neither frozen nor refrigerated must be stored. A conventional dry storage is quite sufficient. Thus, not only the application but also the storage are significantly simplified and cost-reduced possible.

As dry fruit pulp in particular dried banana marc is used, but it is also possible to use other crops or vegetables. In particular, dry yeast is used as the yeast. The advantageous dry attractant combination can be activated with water. The fruit pulp and the yeast release volatile fermentation products, which have an attracting effect on fruit flies. Due to the specific for Drosophilidae or Drosophila aggregation pheromone this effect is further enhanced. In addition, the trap equipped with such an attractant combination is effective immediately after its activation, since the aggregation pheromone immediately unfolds its attraction due to its high vapor pressure, during which the fermentation of the pulp and the yeast must first begin and their effect thus occurs somewhat delayed.

The attractant combination according to the invention thus not only a particularly attractive and attractive effect on Drosophilidae or Drosophila is also practiced with long range, but is also given a particularly storable and stable attractant combination. In addition, the attractant combination according to the invention is a purely biological preparation which is particularly suitable for the sensitive foodstuffs sector.

Also takes place during storage and use no odor for people.

Further advantageous embodiments of the dry attractant combination of the invention are described in the subclaims 2 to 9.

In a preferred embodiment of the dry attractant combination, it is advantageous if the fruit pulp and yeast are present in a certain ratio to each other. In this way the fermentation and the resulting attraction are optimized. Such an advantageous concentration is characterized in the features of claim 2.

According to another preferred embodiment, it is advantageous that the aggregation pheromone is immobilized with a certain amount of silica. The advantageous ratios of silica to aggregation pheromone according to the invention are the subject of the features of claim 3.

A particularly effective and advantageous attractant combination according to the invention given by the features of claim 4.

To further improve the attractiveness further attractants according to the features of claim 5 are added according to the invention.

To improve the capture insecticides according to the features of claim 6 are added according to the invention.

In order to further improve the shelf life, further antioxidants according to the invention, in particular vitamin C, according to the features of claim 7 admit.

For the yeast or dry yeast used, it is possible to scoop from different yeast strains, but particularly advantageous is the use of a Drosophila own yeast. From such a yeast goes for Drosophila special attraction.

The dry attractant combination according to the invention according to the features of claim 9 in the form of a dry free-flowing powder or as tablets. In this way, the attractant combination can be easily dosed and handled and also easily and without being spilled into a trap.

The invention also relates to a preparation for using the dry attractant combination. According to the features of claim 10, the preparation consists of a suspension or solution of the dry attractant combination in a liquid, in particular water, wherein the liquid is preferably 90 to 99% by weight, based on the total weight of the activated attractant combination. The liquid, especially water, moisturises the pulp and activates the yeast, gradually causing fermentation. In this case, an immediate luring effect is achieved with the aid of immobilized in silica aggregation pheromone.

The invention is also a case, as characterized according to the features of claim 11, wherein the case itself may be a commercial case for fruit flies or similar flies.

It is a further object of the invention to provide a process by means of which the attractant combination can be carried out quickly, simply and, above all, avoiding losses of the attractants.

This method is characterized by the features of claim 12, wherein it depends crucially on immobilizing or mixing the aggregation pheromone with silicon dioxide. If this is immobilized too slow, then it comes to heavy losses by evaporation of aggregation pheromone. The inventive method describes an advantageous embodiment, which ensures a loss-free production of the dry attractant combination.

The preparation of an exemplary dry attractant combination for Drosophila requires in a first step, a dilution of the 99% synthetic aggregation pheromone cVA. It is known from the prior art to dilute cVA with hexane as a solvent. However, since hexane is toxic and has no particular attraction to Drosophila, the dilution according to the method of the invention is advantageously carried out with ethanol. In this ethanol solution also other liquid or volatile attractants can be added.

Ethanol has the decisive advantage, especially in the food industry, to be less toxic than hexane. In addition, ethanol has a strong attractant to Drosophila, which is absent in hexane. Ethanol thus interacts with the aggregation pheromone combinatorially and enhances the overall effect of the attractant combination.

Subsequently, silicon dioxide or Aerosil is presented in a mortar. The ethanol solution containing the attractants is then added to the silica. This is done by first adding the entire amount of attractant solution to the silica and mixing or triturating immediately after this addition. This mixing or Verreibungsvorgang must be carried out quickly to lose as little pheromone or volatile attractants by evaporation. The addition of the attractant solution can take place at a single point in the silica or evenly distributed in several places.

Subsequently, the other dry ingredients or the attractants, namely the dry fruit pulp and the dry yeast and optionally further dry additives or active ingredients added.

In the following examples of advantageous compositions of a dry attractant combinations are given.

The following starting materials have been used by way of example but not by way of limitation: Freeze-dried banana powder, in particular banana, fd Art. No. 72406 from Freeze-Dry Foods GmbH, Greven, Germany, is used as dry fruit pulp used.

For example, dry baker's yeast, in particular dry baker's yeast IstaFerm from Eagle LALLEMAND INC., Is used as dry yeast. Montreal, QC Canada.

The Drosophila aggregation pheromone is also referred to as cis-vaccenyl acetate or (Z) -11-octadecenyl acetate or cVA. In particular, cVA from Plant Research International B.V. Pherobank, Wageningen, the Netherlands, is used with a purity of about 99%.

Silicon dioxide or silica is used in the form of Aerosil® in any powdery form and grain size, in particular silicon dioxide with a molecular weight of 60:08, and SiC> IICSaS6S patenui it AT10 665 U1 2009-08-15 g / mol highly dispersed and pure of the company Merck KgaA, Darmstadt, Germany.

Table 1 shows a particularly preferred dry first attractant combination I. TABLE 1: DRY LOCKING COMBINATION I.

Component Weight (mg) Weight Percent Dry Fruit Marrow 3000 96.8 Yeast 50 1.6 Aerosil 50 1.6 Ethanol 0.035 0.00113 cVA Traces 1.3 E-07 Total 3100.035 100

Table 2 shows preferred concentration ranges of the first attractant combination I. TABLE 2: DRY LOCKING COMBINATION I. - AREAS

Component Value Range (%) Preferable Range (%) Dry Fruit Pulp 70-99 90-98 Yeast 0.01-10 1.2-2 Aerosil 1-30 1.5-5 Ethanol 0.0009-2 0.001-0.01 cVA (99% pure) 6.50361E-08 l, 95076E-06 9.75537E-08 3.90205E-07 5/9 ijitirriiWiSF'fiS patenmamt AT10 665 U1 2009-08-15 Table 3 shows another preferred dry attractant combination II in addition acetic acid is included.

TABLE 3: DRY LOCKING COMBINATION II

Component Weight (mg) Weight Percent Dry Fruit Marrow 3000 95.2 Yeast 50 1.59 Aerosil 50 1.59 Ethanol 0.035 0.00111 cVA Traces Traces Acetic Acid 50 1.59 Total 3150.035 100 Table 4 shows another preferred dry one Attractant combination III in which additional acetic acid and dimilin are included.

TABLE 4: DRY LOCKING COMBINATION III

Component Weight (mg) Weight Percent Dry Fruit Marrow 3000 89.6 Yeast 50 1.49 Aerosil 50 1.49 Ethanol 0.035 0.001 cVA Trace Traces Acetic Acid 50 1.49 Dimilin 200 5.97 Total 3350.035 100 6/9

Claims (12)

Table 5 shows a water-activated attractant combination I of the dry attractant combination I. TABLE 5: ACTIVATED LOCKING COMBINATION I Component Weight (mg) Weight Percent Dry Cargass 3000 3.91 Yeast 50 0.0485 Aerosil 50 0.0485 Ethanol 0.035 3.395E-05 cVA Traces Lanes Water 100.000 97 Total 103.100.035 100 Claims 1. Dry attractant combination for Drosophilidae or fruit flies, in particular of the genus Drosophila, containing as attractants - dry fruit pulp, in particular dried or freeze-dried banana marrow, dried yeast or dry yeast, at least one aggregation pheromone active for Drosophilidae, in particular for Drosophila species, in particular cis-vaccenyl acetate cVA, immobilized with silicon dioxide. 2. Dry attractant combination according to claim 1, containing - 70 to 99 wt -.%, In particular 90 to 98 wt -.%, Dry fruit pulp and - 0.01 to 10 wt -.%, In particular 1.2 to 2 wt -. %, Yeast, based on the total weight of the dry attractant combination. 3. Dry attractant combination according to claim 1, containing -1 to 30% by weight, in particular 1.5 to 25% by weight, silicon dioxide, and - 6,5x10 8 to 2 × 10 -6% by weight, in particular 9, 8x10 "8 to 3.9x10'7 weight percent, aggregation pheromone, based on the total weight of the dry attractant combination. 7/9 ijitirriiWiSF'fiS patenmamt AT10 665 U1 2009-08-15 4. Dry attractant combination according to any one of claims 1 and 3, containing 96 wt .-% dry fruit pulp, 1.6 wt .-% yeast, 1.6 wt .-% silica, and 1.3 x 10'7 wt .-% aggregation pheromone, based on the total weight of the dry attractant combination. 5. Dry attractant combination according to one of claims 1 to 4, additionally containing further attractants selected from the group of volatile short-chain carboxylic acids, volatile short-chain alcohols, volatile aryl-substituted alcohols, organic nitrogen compounds, acetates, sugars, especially ethanol, ethyl acetate, butyl acetate or isoamyl acetate, Styrene, indole, 2-heptanol, 2-methylbutyl-2-methyl butanoate, (Z) -4-hexenyl butanoate, various hexanoates, linalool, eugenol, 2-phenylethanol, 1,2-dimethoxy-4- (2-propenyl) Benzene, phenylmethyl acetate and / or acetic acid, in particular in amounts of 0.05 to 5 wt .-% based on the total weight of the dry attractant combination. 6. Dry attractant combination according to one of claims 1 to 5, containing at least one insecticidal active ingredient, in particular a moulting inhibitor, preferably diflubenzuron, in particular in amounts of 4 to 10 wt .-% based on the total weight of the dry attractant combination. 7. Dry attractant combination according to one of claims 1 to 6, containing antioxidants, in particular vitamin C, in particular in amounts of 1 to 5 wt .-% based on the total weight of the dry attractant combination. 8. The dry attractant combination according to any one of claims 1, 2 and 4, wherein the yeast is a yeast occurring on Drosophila flies. 9. Dry attractant combination according to one of claims 1 to 8, wherein the attractant combination is in the form of a dry, free-flowing powder or in tablet form. 10. Preparation containing the dry attractant combination according to one of claims 1 to 9, characterized in that the preparation consists of a suspension or solution of the dry attractant combination in a liquid, in particular water, wherein the liquid is preferably 90 to 99 wt .-%, based on the total weight of the dry attractant combination. 11. trap for Drosophilidae or Taufliegen, in particular the genus Drosophila, in which the dry attractant combination according to any one of claims 1 to 9 or the preparation of the dry attractant combination according to claim 10 is arranged. 12. A process for preparing a dry attractant combination according to any one of claims 1 to 9, wherein at least one Drosophilidae, in particular for Drosophila, arteigen acting aggregation pheromone, in particular cis-Vaccenylacetat cVA, in a solvent, in particular ethanol, is dissolved, then silica, preferably in a mortar, and the aggregation pheromone solution, and optionally other liquid or volatile attractants, preferably acetic acid or an acetate, is added to or in the silica, AT10 665 U1 2009-08- 15 wherein first the entire amount of the solution to the silica, in a single place or evenly distributed in several places, is added and then everything is mixed or triturated, the mixing or Verreibungsvorgang immediately, especially within a few seconds, after the Addition takes place or wherein the mixing bz w.Verreibungsvorgang occurs simultaneously with the addition of the solution to the silica, and wherein finally to the thus immobilized aggregation pheromone dry fruit pulp, especially dried or freeze-dried banana marc, and dried yeast or dry yeast and optionally other dry attractants are mixed. For this no drawings 9/9