SI22522A2 - Dry combination of lure substances for vinegar flies - Google Patents

Abstract

Dry combination of lure substances for Drosophilidae or vinegar flies, especially of the genus Drosophila, which contains as lure substances: - dry fruit pulp, particularly dried or liophylized banana pulp; - dried yeast or dry yeast; - at least one aggregation feromone specific for Drosophilidae, particularly for Drosophila, particularly cis-vaccenyl acetate cVA dissolved in a solvent and immobilized by silicon dioxide.

Description

DRY COMBINATION OF VASING MUSCLES FOR WINE MUSCLES

The present invention relates to a dry combination of wine fly lures according to claim 1, an activated combination of lure substances according to claim 11, and also to a wine fly fly trap according to claim 12.

The present invention falls within the scope of insect inviting substances, in particular Drosophilidae flies and Drosophila flies.

The family of wine flies or. Drosophilidae, also known as fruit or acetic flies, or dew flies, are in the order of two-leafed flies. These are small, about 2mm large flies that are almost everywhere, both in forests and in residential areas.

The term fruit fly derives from its tendency to rot. The name dew fly, however, is derived from its behavior because these animals mainly fly in the morning and evening.

There are about 3000 species known in the world that live in different ecological areas. The family of wine flies or. Drosophilidae comprises the subfamily Drosophilinae. One genus of Drosophilinae is Drosophila. This genus has about 1500 different species, of which Drosophila melanogaster is best known. Drosophila melanogaster is also known as the black belly wine fly and is one of the most studied organisms in the world. Further common species are Drosophila immigrans or Drosophila hydei.

Flies, as pests, are a major problem in many areas of the food industry. Especially during the warm season, the mass appearance of these insects occurs. Due to the active harvesting of fly larvae and the transmission of harmful microorganisms, food spoilage is greatly accelerated. Due to their huge reproductive capacity, these flies can reproduce en masse in a very short time. Fruit flies cause considerable damage and impair human well-being.

Wine flies are one of the most important pests in the food field. Some species are typically companions of cultures that preferentially linger in and around human environments to primarily attack fruits and vegetables. Until a few years ago, these species were not known as pests, but as non-harmful pests. EU hygiene regulation 2004 EC VO (EG) Nr. 852/2004 clearly classifies fruit flies as pests and should also be properly suppressed.

Fruit flies are known to be particularly attracted to mature and decomposing plant material because they are used to lay their eggs and reproduce them. As a signpost for these litters, the flies are volatile matter derived from organic material.

Numerous methods and inviting substances are known in the art. destroying Drosophila flies. Especially in the food sector and in supermarkets, there is a need to set such traps. combinations of inviting substances that do not have any adverse or harmful effects on the food itself. In most cases, this is achieved by traps containing liquid substances.

Thus, e.g. from US 6,543,181 a known trap with a liquid combination of inviting substances comprising short-chain carboxylic acids, alcohols and nitrogen-containing substances.

The use of fruit marrow or yeast as a lure for Drosophila is also known.

For some insects, e.g. kitchen cockroaches, the use of aggregation pheromones as inviting agents is also known.

The disadvantage of using fresh fruit jelly is, of course, that it is easily perishable, and thus traps quickly lose their effect and, on the other, it is not possible to store such a combination of inviting substances containing fresh fruit jelly.

Also, most of the inviting substances known in the art are highly volatile at room temperature and can only be added before the traps are activated so that they do not evaporate prematurely. Therefore, it is not possible to store such liquid combinations of inviting substances well.

Therefore, it is an object of the present invention to prepare a combination of inviting substances for Drosophilidae or. wine flies, especially for the genus Drosophila, which would be particularly effective but at the same time easy to use and suitable for storage.

The task is solved by the characteristic of claim 1.

The characteristic of claim 1 describes a dry combination of inviting substances comprising a fruit marrow and a yeast in the dry form and an aggregation pheromone specific for Drosophilidae or. Drosophila, which is immobilized with silica.

In this way, a combination of inviting substances is carried out, which is suitable for long storage, is stable and also not susceptible to deterioration and is easily used, filled and stored due to its dry consistency. The normally volatile inviting substance, in this case the aggregation pheromone, is immobilized on silica so that no evaporation of the pheromone occurs. In this way, we can also provide a well-defined and even over a long period of time the same conserved concentration of individual inviting substances relative to one another. The combination of the invitation materials does not change either their effectiveness or the concentration of the individual invitation substances with respect to each other during storage. A further advantage of such a dry combination of invitations is that, unlike liquid combinations of invitations, they do not need to be stored frozen or chilled. Conventional dry storage is completely sufficient. Thus, not only is it possible to dramatically simplify and reduce the use, but also to store it.

In particular, we use dried banana kernels as dried fruit kernels, but other types of fruits or vegetables can also be used. Dry yeast is especially used as yeast. Preferably the dry combination of the inviting substances can be activated with water. Fruits and yeasts release volatile fermentation products that have an inviting effect on fruit flies. Due to the aggregation pheromone specific for Drosophilidae or. Drosophila, this effect is further amplified. In addition, a trap equipped with such a combination of inviting substances is effective immediately after activation, because the aggregation pheromone immediately develops its inviting effect due to its high vapor pressure, whereas in fruit pulp and yeast, fermentation must first begin and thus have a somewhat temporary effect detained.

The combination of the inviting substances of the present invention not only achieves a particularly attractive and attractive effect for Drosophilidae. Drosophila, which also has a large action radius, but also prepares a combination of inviting substances that is particularly well-kept and stable.

In addition, the combination of inviting substances of the invention is a purely biological preparation, which is particularly suitable for the sensitive food field.

It also does not create unpleasant odors for humans when stored and used.

Further preferred forms of the invention are described in the dependent claims.

In the preferred embodiment of the dry combination of the inviting substances, it is preferable that the fruit pulp and the yeast are in a certain relation to each other. Thus, the fermentation and the inviting effect that results are optimized. Such a preferred concentration is described in the features of claim 2.

According to a further preferred embodiment, it is preferred that the aggregation pheromone to be concentrated in its pure form be dissolved with a given amount of solvent and immobilized with a certain amount of silica. Preferred silica to solvent and aggregation pheromone ratios are described with the characteristic of claim 3.

A particularly effective and preferred combination of inviting substances is indicated by the characteristic of claim 4.

To further enhance the attractiveness, further inviting substances may be added according to the characteristics of claim 5.

Insecticides according to the characteristics of claim 6 can be added to improve the catching effect.

In order to improve storage suitability, it is further possible to add antioxidants, especially vitamin C, according to the characteristics of claim 7.

According to a preferred embodiment of the Drosophilidae- or. DravopAZ / α-aggregation pheromone is not dissolved in hexane as known in the art but in ethanol. In particular, it has a decisive advantage for the food industry because it is less toxic than hexane. Additionally, ethanol has a strong inviting effect on Drosophila, which is absent in hexane. Ethanol thus acts in combination with the aggregation pheromone and amplifies the combined effect of the combination of the inviting substances.

For the yeast used. Dry yeast can be obtained from a variety of yeast sources, and of course the use of the characteristic yeast of Drosophila is particularly preferred. Such a yeast results in a specific catching effect for Drosophila.

The dry combination of the inviting substances is preferably in the form of a dry powder, such as tablets or the like. Thus, the combination of the inviting substances can be easily dosed and used and easily and without spillage can be trapped.

Activation of the dry combination of the inviting substances is preferably accomplished by the addition of a certain amount of liquid, especially water, according to claim 11. Thus, the fruit pulp becomes moist, the yeast is activated and fermentation gradually occurs. In order to achieve an immediate invitational effect, an aggregation pheromone immobilized in silica is added at the same time, and immediately after activation, the invitational effect resulting from it is developed.

Such a dry or activated combination of inviting substances may be carried out in commercially-customized traps for wine flies or similar flies.

The dry combination of the inviting substances of the invention can be prepared by the following process: At least one aggregation pheromone specific for Drosophilidae, especially for Drosophila, especially cis-vaccinated acetate cVA, is dissolved in a defined amount of solvent, especially ethanol. For example, silicon dioxide is added to the mortar and a defined amount of the aggregation pheromone solution is added, and optionally further liquid or liquid. volatile invitation substances, preferably acetic acid or acetate to silica or. into it. First, the whole amount of the solution is added to the silica in one place or evenly distributed in several places, and only then all are mixed or mixed. get old, with the process of mixing or mixing. third directly, especially within a few seconds, after addition, or by performing the mixing or mixing process. the third at the same time as the solution was added to silica. Then, the immobilized aggregation pheromone is mixed with dried fruit pulp, especially dried or dried. freeze-dried banana meat, and also dried yeast or. dried yeast, and also optionally other dried invitation materials.

In this case, immobilization and / or immobilization are crucial. of mixing aggregation pheromone with silica. However, if immobilization is too slow, large losses are caused by the evaporation of the aggregation pheromone. This process describes a preferred embodiment that ensures the preparation of a dry combination of invitational substances without loss.

The preparation of a typical dry combination of inviting substances for Drosophila requires, in the first step, a dilution of 99% of the synthetic cVA aggregation pheromone. It is known from the prior art to dilute cVA with hexane as a solvent. However, since hexane is toxic and does not have any particular inviting effect on Drosophila, dilution by the process is preferably carried out with ethanol. Further ethanol or liquid can also be added to this ethanol solution. volatile invitation materials.

Next, silica or silica is added. aerosil to the mortuary. A solution of ethanol containing the invitation materials is then added to silica. This is done by first adding the whole amount of the solution of the solids to the silica and immediately after the addition, mix or mix. we're getting old. This mixing or mixing process. the third one should be done quickly so that as little of the pheromone or as possible is lost. volatile volatiles due to evaporation. The addition of the solution of the solids into silica may be carried out in a single place or evenly distributed in several places.

Subsequently, further dry ingredients are added. inviting substances such as dried fruit marrow and dried yeast, as well as optionally further dried additional substances or active ingredients.

The following are examples of preferred compositions of a dry combination of inviting substances. Typically, the following starting materials are used, which are by no means limiting.

For example, as a dry fruit marrow, e.g. freeze-dried banana powder, especially bananas, fd Art. no. 72406 from Freeze-Dry Foods GmbH, Greven, Germany.

Typically, dry yeast is used e.g. dried baker's yeast, especially IstaFerm's baked yeast from Eagle LALLEMAND INC. Montreal, QC Canada.

The drosp / zz / chz-aggregation pheromone is also referred to as cis-vaccinated acetate or. (Z) -lloctadecenyl acetate or. cVA. Particularly, we use Plant Research International's B. V. Pherobank cVA, Wageningen, The Netherlands with a purity of about 99%.

Silicon dioxide or. silica is used in the form of Aerosil® in any powder form and grain size, especially silica with a molecular weight of 60.08 g / mol, highly dispersed and purest from Merck KgaA, Darmstadt, Germany.

Table 1 shows a particularly preferred dry first combination of bait materials I.

Table 1; Dry combination of invitation materials I

Component Weight (mg) Mass fraction (%) dried fruit pulp 3000 96.8 yeast 50 1.6 aerosil 50 1.6 ethanol 0.035 0,00113 cVA traces 1.3 E-07 total 3100,035 100

Table 2 shows the preferred concentration range of the first I-combination.

Table 2: Dry combination of invitation substances I - zones

Component Variable range (%) Priority area (%) dried fruit pulp 70-99 90-98 yeast 0,01-10 1,2-2 aerosil 1-30 1.5-5 ethanol 0,0009-2 0,001-0,01 cVA (99% pure) 6,50361 E-08- 1,95076 E-06 9,75537 E-08- 3.90205 E-07

Table 3 shows a further preferred dry combination of invitation materials II additionally containing acetic acid.

Table 3: Dry combination of invitation materials II

Component Weight (mg) Mass fraction (%) dried fruit pulp 3000 95,2 yeast 50 1.59 aerosil 50 1.59 ethanol 0.035 0,00111 cVA traces traces acetic acid 50 1.59 total 3150,035 100

Table 4 shows a further preferred dry combination of invitation materials III, additionally containing acetic acid and dimilin.

Table 4: Dry combination of invitation materials III

Component Weight (mg) Mass fraction (%) dried fruit pulp 3000 89,6 yeast 50 1.49 aerosil 50 1.49 ethanol 0.035 0.001 cVA traces traces acetic acid 50 1.49 dimilin 200 5.97 total 3350,035 100

Table 5 shows the combination of water-activated invitations I, the dry invitations I.

Table 5: Activated combination of invitation materials I

Component Weight (mg) Mass fraction (%) dried fruit pulp 3000 2.91 yeast 50 0,0485 aerosol 50 0,0485 ethanol 0.035 3,395 E-05 cVA traces traces water 100 000 97 total 103 100,035 100

Claims (12)

1. Dry combination of inviting substances for Drosophilidae or. wine flies, especially of the genus Drosophila, containing as inviting substances: - dried fruit pulp, especially dried or dried. lyophilized banana meat; - dried yeast or. dried yeast; - at least one aggregation pheromone specific for Drosophilidae, especially for Drosophila, especially cis-vaccinated acetate cVA dissolved in solvent and immobilized with silica. A dry combination of inviting substances according to claim 1, comprising: - 70-99 wt. %, especially 90-98 wt. % dry fruit kernels, and - 0.01-10 wt. %, especially 1.2-2 wt. % yeast, based on the total weight of the dry combination of the baits. A dry combination of inviting substances according to one of the preceding claims, comprising: - 1-30 wt. %, especially 1.5-25 wt. % silica; - 0,0009-2 wt. %, especially 0.001-0.01 wt. % of the immobilized solvent, and - from 6,5 χ 10 ⁸ wt. % to 2 x 10 “ ⁶ wt. %, especially of 9.8 x 10 " ⁸ wt. % to 3.9 x 10 ⁷ wt. % of the aggregation pheromone dissolved in the solvent, based on the total weight of the dry combination of the inviting substances. A dry combination of inviting substances according to one of the preceding claims, comprising: - about 96 wt. % dry fruit kernels; - about 1.6 wt. % yeast; - about 1.6 wt. % silica; - about 0.0011 wt. % of the immobilized solvent and - approximately 1,3 χ 10 ⁷ wt. % of the aggregation pheromone dissolved in the solvent, based on the total weight of the dry combination of the inviting substances. 5. A dry combination of inviting substances according to any one of the preceding claims, containing further further inviting substances such as volatile short-chain carboxylic acids, volatile short-chain alcohols, volatile aryl-substituted alcohols, organic nitrogen compounds, acetates, sugar, in particular ethanol, ethyl acetate, butyl acetate or isoamyl acetate, styrene, indole, 2-heptanol, 2-methylbutyl-2-methyl butanoate, (Z) -4-hexenyl butanoate, various hexanoates, linalool, eugenol, 2-phenylethanol, 1,2-dimethoxy-4- (2 -propenyl-benzene, phenylmethylacetate and / or acetic acid, in particular in an amount of 0.05-5% by weight. % based on the total weight of the dry combination of the baits. A dry combination of inviting substances according to any one of the preceding claims, comprising at least one insecticidal agent, especially a levitation inhibitor, preferably diflubenzuron, especially in an amount of 4-10 wt. % based on the total weight of the dry combination of the baits. A dry combination of inviting substances according to one of the preceding claims, containing antioxidants, in particular vitamin C, in particular in an amount of 1-5 wt. % based on the total weight of the dry combination of the baits. The dry combination of the inviting substances according to one of the preceding claims, wherein the solvent is ethanol. A dry combination of inviting substances according to one of the preceding claims, wherein the yeast is Drosophila characteristic yeast. A dry combination of inviting substances according to one of the preceding claims, wherein the combination of inviting substances is in the form of a dry powder, in the form of tablets, packaged in bags or gelatin capsules or the like. An activated invitation material combination comprising a dry invitation material combination according to one of the preceding claims absorbed or absorbed. suspended or. dissolved in a liquid, especially water, wherein the liquid is preferably 90-99 wt. % based on the total weight of the activated combination of invitation materials. 12. A trap for Drosophilidae or. wine flies, especially of the genus Drosophila, in which a dry combination of inviting substances according to one of claims 1-10 or an activated combination of inviting substances according to claim 11 is arranged.