AR128277A1 - Monitoreo de patógenos en cultivos y predicción de poblaciones - Google Patents
Monitoreo de patógenos en cultivos y predicción de poblacionesInfo
- Publication number
- AR128277A1 AR128277A1 ARP230100098A ARP230100098A AR128277A1 AR 128277 A1 AR128277 A1 AR 128277A1 AR P230100098 A ARP230100098 A AR P230100098A AR P230100098 A ARP230100098 A AR P230100098A AR 128277 A1 AR128277 A1 AR 128277A1
- Authority
- AR
- Argentina
- Prior art keywords
- reads
- sample
- samples
- resistance
- population
- Prior art date
Links
- 238000012544 monitoring process Methods 0.000 title 1
- 244000052769 pathogen Species 0.000 title 1
- 238000000034 method Methods 0.000 abstract 3
- 239000000523 sample Substances 0.000 abstract 3
- 239000012472 biological sample Substances 0.000 abstract 2
- 230000002538 fungal effect Effects 0.000 abstract 2
- 230000003032 phytopathogenic effect Effects 0.000 abstract 2
- 238000012300 Sequence Analysis Methods 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 238000003491 array Methods 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 abstract 1
- 238000003908 quality control method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Mycology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La presente invención se refiere a un método para identificar una población de fitopatógenos que incluye variantes en un campo o invernadero, comprendiendo el método: a) recolectar un conjunto de muestras en un lugar que comprende al menos parte de un organismo fitopatógeno nocivo; b) procesar el conjunto de muestras; y analizar cada muestra mediante un análisis de secuencia de metagenoma completo, produciendo así lecturas de metagenoma completo; c) secuenciar ADN y/o ARN del organismo nocivo y determinar una o más secuencias de ADN y/o ARN; d) opcionalmente, someter las lecturas del metagenoma completo a procedimientos de control de calidad que comprenden la eliminación de lecturas no fitopatógenas; e) comparar las lecturas restantes con una o más matrices de k-meros de hongos conocidos mediante la descomposición de las lecturas en un conjunto de k-meros derivados de muestras de aproximadamente 300 pares de bases; y f) analizar las tablas de lectura del modo k-mer para determinar la muestra biológica basándose en el análisis comparativo de k-mer con subtipos de hongos conocidos u otras muestras biológicas con resultados o composición taxonómica conocidos; y g) introducir información sobre la resistencia de la población de fitopatógenos determinada, que comprende cada variedad individual determinada en la muestra, así como la ocurrencia de cada variedad individual en un mapa de calor de resistencia y/o un módulo de predicción de resistencia.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP22152293 | 2022-01-19 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR128277A1 true AR128277A1 (es) | 2024-04-10 |
Family
ID=79730171
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP230100098A AR128277A1 (es) | 2022-01-19 | 2023-01-16 | Monitoreo de patógenos en cultivos y predicción de poblaciones |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN118591617A (es) |
| AR (1) | AR128277A1 (es) |
| WO (1) | WO2023139111A1 (es) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201609221D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
-
2023
- 2023-01-16 AR ARP230100098A patent/AR128277A1/es unknown
- 2023-01-18 WO PCT/EP2023/051106 patent/WO2023139111A1/en active Application Filing
- 2023-01-18 CN CN202380017674.XA patent/CN118591617A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN118591617A (zh) | 2024-09-03 |
| WO2023139111A1 (en) | 2023-07-27 |
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