AR124149A1 - Método para producir plantas de papa con pardeamiento suprimido mediante el sistema crispr / cas9 - Google Patents
Método para producir plantas de papa con pardeamiento suprimido mediante el sistema crispr / cas9Info
- Publication number
- AR124149A1 AR124149A1 ARP210103261A ARP210103261A AR124149A1 AR 124149 A1 AR124149 A1 AR 124149A1 AR P210103261 A ARP210103261 A AR P210103261A AR P210103261 A ARP210103261 A AR P210103261A AR 124149 A1 AR124149 A1 AR 124149A1
- Authority
- AR
- Argentina
- Prior art keywords
- potato
- gene
- stppo2
- nucleotide sequence
- browning
- Prior art date
Links
- 244000061456 Solanum tuberosum Species 0.000 title abstract 10
- 108091033409 CRISPR Proteins 0.000 title abstract 4
- 238000010354 CRISPR gene editing Methods 0.000 title abstract 2
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 235000002595 Solanum tuberosum Nutrition 0.000 abstract 6
- 108090000623 proteins and genes Proteins 0.000 abstract 5
- 239000002773 nucleotide Substances 0.000 abstract 4
- 125000003729 nucleotide group Chemical group 0.000 abstract 4
- 102000004389 Ribonucleoproteins Human genes 0.000 abstract 3
- 108010081734 Ribonucleoproteins Proteins 0.000 abstract 3
- 108010042407 Endonucleases Proteins 0.000 abstract 2
- 102000004533 Endonucleases Human genes 0.000 abstract 2
- 108020005004 Guide RNA Proteins 0.000 abstract 2
- 235000003869 genetically modified organism Nutrition 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 230000035772 mutation Effects 0.000 abstract 2
- 102000030523 Catechol oxidase Human genes 0.000 abstract 1
- 108010031396 Catechol oxidase Proteins 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 108091027544 Subgenomic mRNA Proteins 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 abstract 1
- 230000037431 insertion Effects 0.000 abstract 1
- 238000003780 insertion Methods 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
- 210000001938 protoplast Anatomy 0.000 abstract 1
- 238000005215 recombination Methods 0.000 abstract 1
- 230000006798 recombination Effects 0.000 abstract 1
- 230000008685 targeting Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y114/18—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with another compound as one donor, and incorporation of one atom of oxygen (1.14.18)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Nutrition Science (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
La presente invención se refiere a un método para producir una planta de papa con pardeamiento suprimido utilizando el sistema CRISPR / Cas9. Más específicamente, se refiere a un método para producir una planta de papa con genoma editado con pardeamiento suprimido mediante la transfección de un protoplasto de papa con un complejo de RNP (ribonucleoproteína) que contiene sgRNA que se dirige al gen StPPO2 (polifenol oxidasa 2 de Solanum tuberosum) derivado de la papa. Dado que el método de la presente invención no implica la inserción de un gen extraño e incluye solo una pequeña mutación que apenas se distingue de la mutación natural, se espera que, a diferencia de los cultivos GMO (organismos modificados genéticamente), que requieren una gran cantidad de costo y tiempo para evaluar la seguridad y los impactos medioambientales negativos, se puede ahorrar una gran cantidad de costes y tiempo en la presente invención. Reivindicación 1: Una composición para la edición genómica para suprimir el pardeamiento en una planta de papa caracterizada porque comprende, como principio activo, un complejo de ribonucleoproteína de un RNA guía específico para una secuencia de nucleótidos dirigida en el gen StPPO2 (polifenol oxidasa 2 de Solanum tuberosum) derivado de papa y una proteína endonucleasa; o un vector de recombinación que comprende un DNA que codifica un RNA guía específico para una secuencia de nucleótidos dirigida en el gen StPPO2 derivado de la papa y una secuencia de ácidos nucleicos que codifica una proteína endonucleasa. Reivindicación 2: La composición según la reivindicación 1, caracterizada porque la secuencia de nucleótidos dirigida en el gen StPPO2 consiste en la secuencia de nucleótidos de la SEQ ID nº 5.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020200160474A KR102272557B1 (ko) | 2020-11-25 | 2020-11-25 | CRISPR/Cas9 시스템을 이용한 갈변 억제 감자 식물체의 제조방법 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR124149A1 true AR124149A1 (es) | 2023-02-22 |
Family
ID=76899482
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP210103261A AR124149A1 (es) | 2020-11-25 | 2021-11-25 | Método para producir plantas de papa con pardeamiento suprimido mediante el sistema crispr / cas9 |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP4253541A1 (es) |
| KR (1) | KR102272557B1 (es) |
| CN (1) | CN116615535A (es) |
| AR (1) | AR124149A1 (es) |
| WO (1) | WO2022114692A1 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102272557B1 (ko) * | 2020-11-25 | 2021-07-05 | 주식회사 툴젠 | CRISPR/Cas9 시스템을 이용한 갈변 억제 감자 식물체의 제조방법 |
| CN114686498A (zh) * | 2022-04-22 | 2022-07-01 | 中佳源物种(深圳)生物科技有限公司 | 苹果突变基因及编码蛋白、苹果突变体的制备方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20160087651A (ko) * | 2015-01-14 | 2016-07-22 | 한국과학기술연구원 | 갈변이 방지된 유색감자 및 유색감자의 갈변을 방지하는 방법 |
| EP3419416A1 (en) * | 2016-02-24 | 2019-01-02 | Rijk Zwaan Zaadteelt en Zaadhandel B.V. | Plants showing a reduced wound-induced surface discoloration |
| KR101987663B1 (ko) * | 2017-10-19 | 2019-06-11 | 한경대학교 산학협력단 | 식물체에서 CRISPR/Cas9 시스템을 이용하여 LeMADS-RIN 유전자 편집에 의해 에틸렌 생산을 감소시키는 방법 |
| KR102272557B1 (ko) * | 2020-11-25 | 2021-07-05 | 주식회사 툴젠 | CRISPR/Cas9 시스템을 이용한 갈변 억제 감자 식물체의 제조방법 |
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2020
- 2020-11-25 KR KR1020200160474A patent/KR102272557B1/ko active IP Right Grant
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2021
- 2021-11-22 WO PCT/KR2021/017138 patent/WO2022114692A1/ko active Application Filing
- 2021-11-22 EP EP21898518.2A patent/EP4253541A1/en active Pending
- 2021-11-22 CN CN202180079490.7A patent/CN116615535A/zh active Pending
- 2021-11-25 AR ARP210103261A patent/AR124149A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2022114692A1 (ko) | 2022-06-02 |
| KR102272557B1 (ko) | 2021-07-05 |
| EP4253541A1 (en) | 2023-10-04 |
| CN116615535A (zh) | 2023-08-18 |
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