AR114740A1 - Métodos y composiciones para mejorar la solubilización del fosfato - Google Patents

Métodos y composiciones para mejorar la solubilización del fosfato

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Publication number
AR114740A1
AR114740A1 ARP190102665A ARP190102665A AR114740A1 AR 114740 A1 AR114740 A1 AR 114740A1 AR P190102665 A ARP190102665 A AR P190102665A AR P190102665 A ARP190102665 A AR P190102665A AR 114740 A1 AR114740 A1 AR 114740A1
Authority
AR
Argentina
Prior art keywords
solubilization
microbial species
phosphates
microorganism
coding sequence
Prior art date
Application number
ARP190102665A
Other languages
English (en)
Inventor
Austin Davis-Richardson
Douglas Higgins
Alvin Tamsir
Karsten Temme
Sarah Bloch
James Gerard Lorigan
Shayin Gottlieb
Rosemary Clark
Original Assignee
Pivot Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pivot Bio Inc filed Critical Pivot Bio Inc
Publication of AR114740A1 publication Critical patent/AR114740A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Métodos y sistemas para mejorar la actividad de solubilización del fosfato en un microorganismo. También se proveen microorganismos identificados mediante el uso de los métodos y sistemas de la presente. Los métodos incluyen los pasos, y los sistemas incluyen las instrucciones para identificar una secuencia codificante asociada con la solubilización del fosfato, para modificar la secuencia codificante asociada con la solubilización del fosfato y la relación funcional entre la secuencia codificante modificada y un promotor, e introducir la secuencia codificante alterada asociada con la solubilización de fosfatos bajo el control del promotor en el microorganismo. Las modificaciones en la secuencia codificante puede incluir, por ejemplo, la asignación aleatoria de los codones nativos, la eliminación de secuencias codificantes y la inserción de secuencias reguladoras. Reivindicación 1: Un microorganismo modificado, caracterizado porque comprende una alteración en un gen asociado con la solubilización de fosfatos, en donde dicho gen asociado con la solubilización de fosfatos es nativo para dicho microorganismo, con lo cual dicho microorganismo modificado solubiliza fosfatos con una capacidad o índice mayor en comparación con un microorganismo no modificado de la misma especie. Reivindicación 83: Un método, caracterizado porque comprende: a) proveer una pluralidad de especies microbianas que están asociadas con una planta blanco de interés; b) evaluar la pluralidad de especies microbianas según un parámetro de colonización y la capacidad para solubilizar fosfatos; c) seleccionar una especie microbiana candidato entre la pluralidad de especies microbianas evaluadas; d) caracterizar, en la especie microbiana candidato, un gen seleccionado del grupo que consiste en: una fosfatasa ácida no específica, una fitasa, un gen de la biosíntesis del ácido glucónico, un transportador del ácido glucónico, una gluconato deshidrogenasa y una glucosa deshidrogenasa; e) introducir una o más variaciones genéticas buscadas en la especie microbiana candidato; f) confirmar la integración de dichas una o más variaciones genéticas buscadas en un locus genómico blanco; y g) repetir los pasos d) y e) una o más veces, hasta que la especie microbiana candidato haya adquirido una capacidad mejorada para solubilizar fosfatos.
ARP190102665A 2018-09-21 2019-09-19 Métodos y composiciones para mejorar la solubilización del fosfato AR114740A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201862734777P 2018-09-21 2018-09-21

Publications (1)

Publication Number Publication Date
AR114740A1 true AR114740A1 (es) 2020-10-07

Family

ID=68240797

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP190102665A AR114740A1 (es) 2018-09-21 2019-09-19 Métodos y composiciones para mejorar la solubilización del fosfato

Country Status (9)

Country Link
US (1) US20210345618A1 (es)
EP (1) EP3853343A1 (es)
CN (1) CN112867784A (es)
AR (1) AR114740A1 (es)
AU (1) AU2019345144A1 (es)
BR (1) BR112021005052A2 (es)
CA (1) CA3113021A1 (es)
MX (1) MX2021003306A (es)
WO (1) WO2020061363A1 (es)

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* Cited by examiner, † Cited by third party
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US10968446B2 (en) 2012-11-01 2021-04-06 Massachusetts Institute Of Technology Directed evolution of synthetic gene cluster
EP3322679A4 (en) 2015-07-13 2019-07-10 Pivot Bio, Inc. METHODS AND COMPOSITIONS FOR IMPROVING THE CHARACTERISTICS OF A PLANT
WO2017062412A1 (en) 2015-10-05 2017-04-13 Massachusetts Institute Of Technology Nitrogen fixation using refactored nif clusters
BR112019014378A2 (pt) 2017-01-12 2020-02-11 Pivot Bio, Inc. Métodos e composições para o aprimoramento de traços de plantas
BR112020008035A2 (pt) 2017-10-25 2020-10-27 Pivot Bio, Inc. métodos e composições para aprimorar micróbios geneticamente modificados que fixam nitrogênio
AU2019291824A1 (en) 2018-06-27 2021-01-14 Pivot Bio, Inc. Agricultural compositions comprising remodeled nitrogen fixing microbes
AU2020256175A1 (en) * 2019-04-01 2021-10-28 Valent Biosciences Llc Mycorrhizae and/or bacillus amyloliquefaciens liquid fertilizer compatible formulations
CN112680383A (zh) * 2021-01-28 2021-04-20 山西省农业科学院农业环境与资源研究所 解磷细菌新菌种及其应用
CN112940964B (zh) * 2021-01-29 2023-05-09 西南大学 一种喀斯特槽谷区石漠化土壤改良微生物菌剂及其制备与应用
EP4221501A4 (en) * 2021-09-15 2024-08-07 Total Biotecnologia Ind E Comercio S A AGRICULTURAL COMPOSITION FROM CONSORTIUM BETWEEN AZOSPIRILLUM SP. PSEUDOMONAS SP., METHOD FOR THE PRODUCTION THEREOF AND ITS USE AS PLANT GROWTH PROMOTER FOR AGRICULTURAL APPLICATIONS
CN113732052B (zh) * 2021-09-27 2022-04-26 中科云恒(成都)环境科技有限公司 假单胞菌土壤修复剂及其在修复重金属污染土壤中的应用
CN114774300B (zh) * 2021-12-31 2023-06-30 西北农林科技大学 韩国假单胞菌及应用
CN114410552B (zh) * 2022-03-28 2022-06-17 佛山市玉凰生态环境科技有限公司 一株氧化硫的盖氏假单胞菌及其在黑臭水体空气中降硫应用
CN115161247B (zh) * 2022-08-22 2023-07-28 河南大学 枯草芽孢杆菌201015及其应用
CN117210481A (zh) * 2022-11-01 2023-12-12 上海市农业生物基因中心 NfADH2抗逆基因及其编码氨基酸在提高大肠杆菌渗透胁迫耐受性中的应用
CN116904361B (zh) * 2023-07-14 2024-05-28 中国科学院南京土壤研究所 一种具有解磷能力的贪铜菌属菌株及其应用
CN117467575B (zh) * 2023-11-06 2024-05-14 山东省农业科学院 一株具有耐盐促生功能的羽扇豆布鲁氏菌k6及其应用
CN118165892B (zh) * 2024-05-09 2024-07-05 南京农业大学三亚研究院 一种假单胞菌复合菌剂及其应用

Family Cites Families (4)

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Publication number Priority date Publication date Assignee Title
US7485451B2 (en) 2004-11-18 2009-02-03 Regents Of The University Of California Storage stable compositions of biological materials
IT1398872B1 (it) * 2009-03-23 2013-03-21 Consiglio Nazionale Ricerche Metodo per aumentare la solubilizzazione del fosfato assimilabile dalle piante
US9228240B2 (en) 2010-06-03 2016-01-05 California Institute Of Technology Methods for detecting and quantifying viable bacterial endo-spores
BR112019014378A2 (pt) * 2017-01-12 2020-02-11 Pivot Bio, Inc. Métodos e composições para o aprimoramento de traços de plantas

Also Published As

Publication number Publication date
CA3113021A1 (en) 2020-03-26
MX2021003306A (es) 2021-05-13
CN112867784A (zh) 2021-05-28
WO2020061363A1 (en) 2020-03-26
US20210345618A1 (en) 2021-11-11
AU2019345144A1 (en) 2021-04-15
EP3853343A1 (en) 2021-07-28
BR112021005052A2 (pt) 2021-06-08

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