AR112366A1 - Proceso rápido de purificación de alto rendimiento para el polisacárido capsular de neisseria meningitidis serogrupo x - Google Patents

Proceso rápido de purificación de alto rendimiento para el polisacárido capsular de neisseria meningitidis serogrupo x

Info

Publication number
AR112366A1
AR112366A1 ARP180101762A AR112366A1 AR 112366 A1 AR112366 A1 AR 112366A1 AR P180101762 A ARP180101762 A AR P180101762A AR 112366 A1 AR112366 A1 AR 112366A1
Authority
AR
Argentina
Prior art keywords
specific
solution
followed
supernatant
pellet
Prior art date
Application number
Other languages
English (en)
Inventor
Davinder Gill
Sarmad Hanif
Nitin Kumar
Sandeep Sharma
Original Assignee
Msd Wellcome Trust Hilleman Laboratories Pvt Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Msd Wellcome Trust Hilleman Laboratories Pvt Ltd filed Critical Msd Wellcome Trust Hilleman Laboratories Pvt Ltd
Publication of AR112366A1 publication Critical patent/AR112366A1/es

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/095—Neisseria
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/60—Medicinal preparations containing antigens or antibodies characteristics by the carrier linked to the antigen
    • A61K2039/6031—Proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/70—Multivalent vaccine
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/22—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Neisseriaceae (F), e.g. Acinetobacter

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Epidemiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Polymers & Plastics (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Materials Engineering (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Reivindicación 1: Un proceso de purificación rápido, de alto rendimiento, de polisacáridos capsulares de Neisseria meningitidis serogrupo X, en donde dicho proceso comprende las etapas de: (a) tratar un caldo de fermentación de Neisseria meningitidis serogrupo X mediante agregado de soluciones de sales simples predeterminadas y polvo de sal seca de concentración específica; (b) agregar un volumen específico de solvente orgánico a la solución tratada con sal de la etapa (a); (c) mezclar la solución de la etapa (b) a temperatura ambiente durante una duración específica; (d) llevar a cabo la centrifugación de la solución de la etapa (c) a rpm especificas durante un periodo predeterminado; (e) recolectar el sobrenadante a partir de la solución centrifugada de la etapa (d); (f) someter dicho sobrenadante de la etapa (e) a concentración y diafiltración utilizando una membrana de corte por peso molecular para obtener polisacárido bacteriano parcialmente purificado; (g) precipitar dicho polisacárido bacteriano parcialmente purificado de la etapa (f) con al menos un detergente catiónico bajo condiciones especificas seguido por centrifugación a rpm específicas durante un predeterminado período para obtener un primer pellet; (h) disolver dicho primer pellet en un agente de precipitación inorgánico; (i) agregar a dicho primer pellet disuelto de la etapa (h) al menos un solvente orgánico seguido por incubación en condiciones predeterminadas; (j) someter dicha solución de la etapa (i) a centrifugación en condiciones específicas seguido por recolección de un segundo pellet; (k) disolver dicho segundo pellet en MQW y tratar con reactivos químicos específicos, y solvente orgánico específico, seguido por incubación en condiciones especificas; (l) someter dicha solución incubada de la etapa (k) a centrifugación en condiciones de centrifugación específicas seguido por recolección del sobrenadante; (m) someter dicho sobrenadante de la etapa (l) a filtración por carbón para obtener un filtrado por carbón hasta que se alcance una densidad óptica deseada; (n) filtrar dicho filtrado por carbón de la etapa (m) utilizando un arreglo de filtración PES seguido por concentración y diafiltración en condiciones específicas; (o) filtrar dicho sobrenadante diafiltrado de la etapa (n) utilizando un arreglo de filtración PES específico para obtener polisacárido purificado; (p) almacenar dicho polisacárido purificado de la etapa (o) en condiciones especificas; en donde dicho proceso es económico, escalable y brinda polisacárido purificado de Neisseria meningitidis serogrupo X en un rango de 147 - 323 mg/L de caldo de fermentación.
ARP180101762 2017-06-27 2018-06-26 Proceso rápido de purificación de alto rendimiento para el polisacárido capsular de neisseria meningitidis serogrupo x AR112366A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IN201711022362 2017-06-27

Publications (1)

Publication Number Publication Date
AR112366A1 true AR112366A1 (es) 2019-10-23

Family

ID=64741186

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP180101762 AR112366A1 (es) 2017-06-27 2018-06-26 Proceso rápido de purificación de alto rendimiento para el polisacárido capsular de neisseria meningitidis serogrupo x

Country Status (7)

Country Link
KR (1) KR20200021933A (es)
CN (1) CN110892076A (es)
AR (1) AR112366A1 (es)
BR (1) BR112019027638A2 (es)
RU (1) RU2019142860A (es)
WO (1) WO2019003239A1 (es)
ZA (1) ZA201908281B (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112521520A (zh) * 2020-12-04 2021-03-19 苏州微超生物科技有限公司 脑膜炎球菌荚膜多糖的制备方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2892035C (en) * 2012-11-21 2019-11-05 Serum Institute Of India Ltd. Production of neisseria meningitidis x capsular polysaccharide
US10543266B2 (en) * 2015-07-04 2020-01-28 Bharat Biotech International Ltd. Polysaccharide vaccine formulations and processes for industrial production of bacterial polysaccharides

Also Published As

Publication number Publication date
ZA201908281B (en) 2022-07-27
KR20200021933A (ko) 2020-03-02
RU2019142860A3 (es) 2021-11-08
WO2019003239A1 (en) 2019-01-03
BR112019027638A2 (pt) 2020-07-07
CN110892076A (zh) 2020-03-17
RU2019142860A (ru) 2021-07-27

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