AR112366A1 - RAPID, HIGH-PERFORMANCE PURIFICATION PROCESS FOR CAPSULAR POLYSACCHARIDE FROM NEISSERIA MENINGITIDIS SEROGROUP X - Google Patents
RAPID, HIGH-PERFORMANCE PURIFICATION PROCESS FOR CAPSULAR POLYSACCHARIDE FROM NEISSERIA MENINGITIDIS SEROGROUP XInfo
- Publication number
- AR112366A1 AR112366A1 ARP180101762A AR112366A1 AR 112366 A1 AR112366 A1 AR 112366A1 AR P180101762 A ARP180101762 A AR P180101762A AR 112366 A1 AR112366 A1 AR 112366A1
- Authority
- AR
- Argentina
- Prior art keywords
- specific
- solution
- followed
- supernatant
- pellet
- Prior art date
Links
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/095—Neisseria
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/60—Medicinal preparations containing antigens or antibodies characteristics by the carrier linked to the antigen
- A61K2039/6031—Proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/70—Multivalent vaccine
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/22—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Neisseriaceae (F), e.g. Acinetobacter
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Mycology (AREA)
- Virology (AREA)
- General Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Materials Engineering (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Reivindicación 1: Un proceso de purificación rápido, de alto rendimiento, de polisacáridos capsulares de Neisseria meningitidis serogrupo X, en donde dicho proceso comprende las etapas de: (a) tratar un caldo de fermentación de Neisseria meningitidis serogrupo X mediante agregado de soluciones de sales simples predeterminadas y polvo de sal seca de concentración específica; (b) agregar un volumen específico de solvente orgánico a la solución tratada con sal de la etapa (a); (c) mezclar la solución de la etapa (b) a temperatura ambiente durante una duración específica; (d) llevar a cabo la centrifugación de la solución de la etapa (c) a rpm especificas durante un periodo predeterminado; (e) recolectar el sobrenadante a partir de la solución centrifugada de la etapa (d); (f) someter dicho sobrenadante de la etapa (e) a concentración y diafiltración utilizando una membrana de corte por peso molecular para obtener polisacárido bacteriano parcialmente purificado; (g) precipitar dicho polisacárido bacteriano parcialmente purificado de la etapa (f) con al menos un detergente catiónico bajo condiciones especificas seguido por centrifugación a rpm específicas durante un predeterminado período para obtener un primer pellet; (h) disolver dicho primer pellet en un agente de precipitación inorgánico; (i) agregar a dicho primer pellet disuelto de la etapa (h) al menos un solvente orgánico seguido por incubación en condiciones predeterminadas; (j) someter dicha solución de la etapa (i) a centrifugación en condiciones específicas seguido por recolección de un segundo pellet; (k) disolver dicho segundo pellet en MQW y tratar con reactivos químicos específicos, y solvente orgánico específico, seguido por incubación en condiciones especificas; (l) someter dicha solución incubada de la etapa (k) a centrifugación en condiciones de centrifugación específicas seguido por recolección del sobrenadante; (m) someter dicho sobrenadante de la etapa (l) a filtración por carbón para obtener un filtrado por carbón hasta que se alcance una densidad óptica deseada; (n) filtrar dicho filtrado por carbón de la etapa (m) utilizando un arreglo de filtración PES seguido por concentración y diafiltración en condiciones específicas; (o) filtrar dicho sobrenadante diafiltrado de la etapa (n) utilizando un arreglo de filtración PES específico para obtener polisacárido purificado; (p) almacenar dicho polisacárido purificado de la etapa (o) en condiciones especificas; en donde dicho proceso es económico, escalable y brinda polisacárido purificado de Neisseria meningitidis serogrupo X en un rango de 147 - 323 mg/L de caldo de fermentación.Claim 1: A rapid, high-performance purification process of capsular polysaccharides of Neisseria meningitidis serogroup X, wherein said process comprises the steps of: (a) treating a fermentation broth of Neisseria meningitidis serogroup X by adding salt solutions Simple predetermined and dry salt powder of specific concentration; (b) adding a specific volume of organic solvent to the salt-treated solution of step (a); (c) mixing the solution from step (b) at room temperature for a specific duration; (d) carrying out centrifugation of the solution from step (c) at specified rpm for a predetermined period; (e) collecting the supernatant from the centrifuged solution of step (d); (f) subjecting said supernatant from step (e) to concentration and diafiltration using a molecular weight cutoff membrane to obtain partially purified bacterial polysaccharide; (g) precipitating said partially purified bacterial polysaccharide from step (f) with at least one cationic detergent under specific conditions followed by centrifugation at specific rpm for a predetermined period to obtain a first pellet; (h) dissolving said first pellet in an inorganic precipitation agent; (i) adding to said first dissolved pellet of step (h) at least one organic solvent followed by incubation under predetermined conditions; (j) subjecting said solution from step (i) to centrifugation under specific conditions followed by collecting a second pellet; (k) dissolving said second pellet in MQW and treating with specific chemical reagents, and specific organic solvent, followed by incubation under specific conditions; (l) subjecting said incubated solution from step (k) to centrifugation under specific centrifugation conditions followed by collecting the supernatant; (m) subjecting said supernatant from step (I) to charcoal filtration to obtain a charcoal filtration until a desired optical density is reached; (n) filtering said charcoal filtrate from step (m) using a PES filtration arrangement followed by concentration and diafiltration under specific conditions; (o) filtering said diafiltered supernatant from step (n) using a specific PES filtration arrangement to obtain purified polysaccharide; (p) storing said purified polysaccharide from step (o) under specific conditions; wherein said process is economical, scalable and provides purified polysaccharide from Neisseria meningitidis serogroup X in a range of 147 - 323 mg / L of fermentation broth.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN201711022362 | 2017-06-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR112366A1 true AR112366A1 (en) | 2019-10-23 |
Family
ID=64741186
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP180101762 AR112366A1 (en) | 2017-06-27 | 2018-06-26 | RAPID, HIGH-PERFORMANCE PURIFICATION PROCESS FOR CAPSULAR POLYSACCHARIDE FROM NEISSERIA MENINGITIDIS SEROGROUP X |
Country Status (7)
| Country | Link |
|---|---|
| KR (1) | KR20200021933A (en) |
| CN (1) | CN110892076A (en) |
| AR (1) | AR112366A1 (en) |
| BR (1) | BR112019027638A2 (en) |
| RU (1) | RU2019142860A (en) |
| WO (1) | WO2019003239A1 (en) |
| ZA (1) | ZA201908281B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112521520A (en) * | 2020-12-04 | 2021-03-19 | 苏州微超生物科技有限公司 | Preparation method of meningococcal capsular polysaccharide |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2013349261B2 (en) * | 2012-11-21 | 2017-09-07 | Serum Institute Of India Private Limited | Production of high yields of bacterial polysaccharides |
| MY183392A (en) * | 2015-07-04 | 2021-02-18 | Bharat Biotech Int Ltd | Polysaccharide vaccine formulations and processes for industrial production of bacterial polysaccharides |
-
2018
- 2018-04-26 KR KR1020197037325A patent/KR20200021933A/en not_active Application Discontinuation
- 2018-04-26 RU RU2019142860A patent/RU2019142860A/en not_active Application Discontinuation
- 2018-04-26 BR BR112019027638-6A patent/BR112019027638A2/en unknown
- 2018-04-26 CN CN201880042088.XA patent/CN110892076A/en active Pending
- 2018-04-26 WO PCT/IN2018/050256 patent/WO2019003239A1/en active Application Filing
- 2018-06-26 AR ARP180101762 patent/AR112366A1/en unknown
-
2019
- 2019-12-11 ZA ZA2019/08281A patent/ZA201908281B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BR112019027638A2 (en) | 2020-07-07 |
| CN110892076A (en) | 2020-03-17 |
| RU2019142860A (en) | 2021-07-27 |
| ZA201908281B (en) | 2022-07-27 |
| RU2019142860A3 (en) | 2021-11-08 |
| WO2019003239A1 (en) | 2019-01-03 |
| KR20200021933A (en) | 2020-03-02 |
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