AR111462A1 - Enzimas lactasa con actividad mejorada a bajas temperaturas - Google Patents
Enzimas lactasa con actividad mejorada a bajas temperaturasInfo
- Publication number
- AR111462A1 AR111462A1 ARP180100909A ARP180100909A AR111462A1 AR 111462 A1 AR111462 A1 AR 111462A1 AR P180100909 A ARP180100909 A AR P180100909A AR P180100909 A ARP180100909 A AR P180100909A AR 111462 A1 AR111462 A1 AR 111462A1
- Authority
- AR
- Argentina
- Prior art keywords
- peptide
- concentration
- amino acid
- lactose
- mixture
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
- C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Enzymes And Modification Thereof (AREA)
- Dairy Products (AREA)
Abstract
Reivindicación 1: Un método para producir un producto lácteo que comprende lo siguiente: (a) mezclar un sustrato con base láctea que comprende lactosa en una concentración de al menos 10 g/L y un péptido o un péptido dimérico que muestra actividad de b-galactosidasa en una concentración que fluctúa entre 10 y 55 mg/L; (b) incubar la mezcla a una temperatura que oscila entre 1ºC y 10ºC durante un lapso suficiente para reducir la concentración de lactosa en la mezcla hasta menos de 0,2 g/L. Reivindicación 2: Un método para reducir el contenido de lactosa en un sustrato con base láctea que comprende lo siguiente: (a) mezclar un sustrato con base láctea que comprenden lactosa en una concentración de al menos 10 g/L y un péptido o un péptido dimérico que muestra actividad de b-galactosidasa en una concentración que fluctúa entre 10 y 55 mg/L; (b) incubar la mezcla a una temperatura que oscila entre 1ºC y 10ºC durante un lapso suficiente para reducir la concentración de lactosa en la mezcla hasta menos de 0,2 g/L. Reivindicación 15: Uso de un péptido o péptido dimérico que muestra actividad de b-galactosidasa para producir un producto lácteo con contenido de lactosa reducido a una temperatura que oscila entre 1ºC y 10ºC durante un lapso suficiente para reducir la concentración de lactosa en la mezcla hasta menos de 0,2 g/L, en el cual el péptido o péptido dimérico que muestra actividad de b-galactosidasa es como se detalla a continuación: (i) un péptido que tiene una secuencia de aminoácido seleccionada entre la SEQ ID Nº 22, 33, 14, 7, 9, 11, 30 y 1 o un péptido que tiene una identidad de secuencia de aminoácido de más del 85% con respecto a cualquiera de estas secuencias; (ii) un péptido que tiene una secuencia de aminoácido seleccionada entre la SEQ ID Nº 22, 33, 14, 13, 19, 7, 9, 11, 26 y 27, 30 y 1 o un péptido que tiene una identidad de secuencia de aminoácido de más del 85% con respecto a cualquiera de estas secuencias; (iii) un péptido que tiene una secuencia de aminoácido representada por la SEQ ID Nº 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, o fragmentos enzimáticamente activos de ellas, o una secuencia de aminoácido de una cualquiera de ellas que no tienen más de 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, ó 22 eliminaciones, adiciones o sustituciones de aminoácido.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP17166021 | 2017-04-11 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR111462A1 true AR111462A1 (es) | 2019-07-17 |
Family
ID=58548558
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP180100908A AR111373A1 (es) | 2017-04-11 | 2018-04-11 | Enzimas de lactasa con propiedades mejoradas |
| ARP180100909A AR111462A1 (es) | 2017-04-11 | 2018-04-11 | Enzimas lactasa con actividad mejorada a bajas temperaturas |
| ARP180100907A AR111589A1 (es) | 2017-04-11 | 2018-04-11 | Enzimas lactasas con propiedades mejoradas |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP180100908A AR111373A1 (es) | 2017-04-11 | 2018-04-11 | Enzimas de lactasa con propiedades mejoradas |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP180100907A AR111589A1 (es) | 2017-04-11 | 2018-04-11 | Enzimas lactasas con propiedades mejoradas |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US11525129B2 (es) |
| EP (2) | EP3609910A1 (es) |
| CN (1) | CN110678479A (es) |
| AR (3) | AR111373A1 (es) |
| AU (3) | AU2018251957C1 (es) |
| BR (1) | BR112019021142A2 (es) |
| CA (1) | CA3059275A1 (es) |
| MA (1) | MA50221A (es) |
| WO (1) | WO2018189224A1 (es) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112021016954A2 (pt) * | 2019-02-28 | 2021-11-23 | Dupont Nutrition Biosci Aps | Método para reduzir lactose em temperaturas altas |
| US20220304323A1 (en) * | 2019-08-30 | 2022-09-29 | Chr. Hansen A/S | Production of lactase enzymes using altered regulation strains |
| WO2021170559A1 (en) * | 2020-02-26 | 2021-09-02 | Novozymes A/S | Polypeptide variants |
| CN112979749A (zh) * | 2021-02-26 | 2021-06-18 | 深圳海创生物科技有限公司 | 一种活性环肽、活性环肽组合物及其在制备具有抗氧化作用或抗衰老作用的产品中的应用 |
| WO2023118436A1 (en) * | 2021-12-22 | 2023-06-29 | Novozymes A/S | Method of producing a milk-based product |
| CN114806906B (zh) * | 2022-05-31 | 2023-02-21 | 江南大学 | 一株产中性乳糖酶的乳酸克鲁维酵母 |
| WO2023237360A1 (en) | 2022-06-07 | 2023-12-14 | Chr. Hansen A/S | Improving filterability of lactase by adding anions selected from malate, tartrate, citrate, gluconate, edta or combinations thereof and sterile filtered lactase product obtained |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1261697B1 (en) | 1999-07-09 | 2004-07-28 | Université de Liège | Cold-active beta galactosidase, the process for its preparation and the use thereof |
| EP1227152A1 (en) | 2001-01-30 | 2002-07-31 | Société des Produits Nestlé S.A. | Bacterial strain and genome of bifidobacterium |
| US7608700B2 (en) | 2004-03-08 | 2009-10-27 | North Carolina State University | Lactobacillus acidophilus nucleic acid sequences encoding stress-related proteins and uses therefor |
| US7459289B2 (en) | 2004-03-08 | 2008-12-02 | North Carolina State University | Lactobacillus acidophilus nucleic acid sequences encoding carbohydrate utilization-related proteins and uses therefor |
| RU2278160C2 (ru) | 2004-04-13 | 2006-06-20 | Институт Молекулярной Генетики Российской Академии Наук (Имг Ран) | РЕКОМБИНАНТНЫЙ БЕЛОК LACspCBD, ОБЛАДАЮЩИЙ БЕТА-ГАЛАКТОЗИДАЗНОЙ АКТИВНОСТЬЮ И СПОСОБНОСТЬЮ САМОПРОИЗВОЛЬНО СВЯЗЫВАТЬСЯ С ЦЕЛЛЮЛОЗОСОДЕРЖАЩИМИ СОРБЕНТАМИ, РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК, КОДИРУЮЩАЯ СИНТЕЗ РЕКОМБИНАНТНОГО БЕЛКА LACspCBD, ШТАММ Escherichia coli M15 [pREP4, pLACspCBD] - ПРОДУЦЕНТ РЕКОМБИНАНТНОГО БЕЛКА LACspCBD, СПОСОБ ПОЛУЧЕНИЯ ИММОБИЛИЗОВАННОГО РЕКОМБИНАНТНОГО БЕЛКА LACspCBD НА ЦЕЛЛЮЛОЗЕ И СПОСОБ ФЕРМЕНТАТИВНОГО РАСЩЕПЛЕНИЯ ЛАКТОЗЫ |
| GB0601901D0 (en) | 2006-01-31 | 2006-03-08 | Product and Process | |
| GB0606112D0 (en) | 2006-03-28 | 2006-05-03 | Product and process | |
| WO2008033520A2 (en) | 2006-09-15 | 2008-03-20 | The Regents Of The University Of California | Bifidobacterial gene sequences and their use |
| EP2380988A3 (en) * | 2007-07-10 | 2012-04-11 | Mosanto Technology LLC | Transgenic plants with enhanced agronomic traits |
| CN101932248B (zh) | 2007-12-03 | 2014-11-26 | 诺维信公司 | 产生乳制品的方法 |
| US20090297660A1 (en) | 2008-06-02 | 2009-12-03 | Kraft Food Holdings, Inc. | Cheese Products Containing Galacto-Oligosaccharides And Having Reduced Lactose Levels |
| CN102361974B (zh) * | 2009-02-10 | 2013-11-06 | 哥本哈根大学 | 冷-活跃的β-半乳糖苷酶、其生产方法、以及所述酶的用途 |
| CN101899463B (zh) * | 2010-07-05 | 2012-05-02 | 山东大学 | 一种带有纤维素吸附域的β-半乳糖苷酶及其应用 |
| EP2530148B1 (en) * | 2011-05-30 | 2015-04-29 | B.R.A.I.N. Biotechnology Research And Information Network AG | Novel beta-galactosidases useful for the production of lactose depleted milk products |
| CN103275953B (zh) * | 2013-06-25 | 2014-06-18 | 山东大学 | 一种转苷底物特异性拓宽的β-半乳糖苷酶 |
| WO2015107050A1 (en) | 2014-01-14 | 2015-07-23 | Dsm Ip Assets B.V. | Improved enzyme variants of lactase from kluyveromyces lactis |
| PL2957180T3 (pl) | 2014-06-19 | 2018-08-31 | Chr. Hansen A/S | Sposób wytwarzania fermentowanego produktu mlecznego z ulepszoną kontrolą zakwaszenia po fermentacji |
| WO2017106264A1 (en) * | 2015-12-14 | 2017-06-22 | Dupont Nutrition Biosciences Aps | Method for producing a clean lactase |
| FI3821712T3 (fi) | 2017-01-13 | 2023-01-13 | Parannetulla menetelmällä saatu hapatettu maitotuote | |
| WO2018187524A1 (en) | 2017-04-07 | 2018-10-11 | Dupont Nutrition Biosciences Aps | BACILLUS HOST CELLS PRODUCING β-GALACTOSIDASES AND LACTASES IN THE ABSENCE OF P-NITROBENZYLESTERASE SIDE ACTIVITY |
| US20210032615A1 (en) | 2017-04-11 | 2021-02-04 | Chr. Hansen A/S | Lactase enzymes with improved properties |
| US20210348147A1 (en) | 2018-10-17 | 2021-11-11 | Chr. Hansen A/S | Lactase enzymes with improved properties at acidic ph |
-
2018
- 2018-04-11 MA MA050221A patent/MA50221A/fr unknown
- 2018-04-11 CA CA3059275A patent/CA3059275A1/en active Pending
- 2018-04-11 AU AU2018251957A patent/AU2018251957C1/en active Active
- 2018-04-11 AR ARP180100908A patent/AR111373A1/es unknown
- 2018-04-11 AR ARP180100909A patent/AR111462A1/es unknown
- 2018-04-11 EP EP18716624.4A patent/EP3609910A1/en active Pending
- 2018-04-11 AR ARP180100907A patent/AR111589A1/es unknown
- 2018-04-11 CN CN201880035256.2A patent/CN110678479A/zh active Pending
- 2018-04-11 EP EP21211668.5A patent/EP4011906A3/en active Pending
- 2018-04-11 US US16/604,129 patent/US11525129B2/en active Active
- 2018-04-11 WO PCT/EP2018/059250 patent/WO2018189224A1/en unknown
- 2018-04-11 BR BR112019021142-0A patent/BR112019021142A2/pt unknown
-
2022
- 2022-03-25 AU AU2022202060A patent/AU2022202060A1/en not_active Abandoned
- 2022-11-14 US US17/986,618 patent/US20230235307A1/en active Pending
-
2024
- 2024-04-12 AU AU2024202396A patent/AU2024202396A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20230235307A1 (en) | 2023-07-27 |
| AR111589A1 (es) | 2019-07-31 |
| RU2019134240A3 (es) | 2021-10-22 |
| CA3059275A1 (en) | 2018-10-18 |
| AU2024202396A1 (en) | 2024-05-02 |
| AU2022202060A1 (en) | 2022-04-14 |
| WO2018189224A1 (en) | 2018-10-18 |
| US20210355471A1 (en) | 2021-11-18 |
| CN110678479A (zh) | 2020-01-10 |
| BR112019021142A2 (pt) | 2020-05-12 |
| RU2019134240A (ru) | 2021-05-11 |
| AR111373A1 (es) | 2019-07-03 |
| EP4011906A3 (en) | 2022-09-07 |
| MA50221A (fr) | 2020-07-22 |
| AU2018251957A1 (en) | 2019-10-31 |
| AU2018251957C1 (en) | 2023-09-28 |
| EP4011906A2 (en) | 2022-06-15 |
| EP3609910A1 (en) | 2020-02-19 |
| AU2018251957B2 (en) | 2022-04-07 |
| US11525129B2 (en) | 2022-12-13 |
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