AR091712A1 - PURIFICACION DE INHIBIDOR DE PROTEASA a₁ DERIVADO DE CULTIVO CELULAR - Google Patents

PURIFICACION DE INHIBIDOR DE PROTEASA a₁ DERIVADO DE CULTIVO CELULAR

Info

Publication number
AR091712A1
AR091712A1 ARP130102440A AR091712A1 AR 091712 A1 AR091712 A1 AR 091712A1 AR P130102440 A ARP130102440 A AR P130102440A AR 091712 A1 AR091712 A1 AR 091712A1
Authority
AR
Argentina
Prior art keywords
proteasa
cellular culture
derivative
inhibitor purification
inhibitor
Prior art date
Application number
Other languages
English (en)
Inventor
Dessources Tony
Ownby David
P Zimmerman Thomas
A Hunt Jennifer
Miller Charles
Ranganathan Senthil
Original Assignee
Grifols Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=48748028&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR091712(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Grifols Sa filed Critical Grifols Sa
Publication of AR091712A1 publication Critical patent/AR091712A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121—Serpins
    • C07K14/8125—Alpha-1-antitrypsin
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/306—Extraction; Separation; Purification by precipitation by crystallization
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4703—Inhibitors; Suppressors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/99—Enzyme inactivation by chemical treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

En el presente se describen métodos para purificar un inhibidor de proteasa a₁ derivado de cultivo celular recombinante y eliminar una especie coloreada que purifica conjuntamente con la proteína recA1PI. También se describen métodos para reducir el hierro en el inhibidor de proteasa a₁ derivado de cultivo celular.
ARP130102440 2012-07-25 2013-07-10 PURIFICACION DE INHIBIDOR DE PROTEASA a₁ DERIVADO DE CULTIVO CELULAR AR091712A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261675560P 2012-07-25 2012-07-25

Publications (1)

Publication Number Publication Date
AR091712A1 true AR091712A1 (es) 2015-02-25

Family

ID=48748028

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP130102440 AR091712A1 (es) 2012-07-25 2013-07-10 PURIFICACION DE INHIBIDOR DE PROTEASA a₁ DERIVADO DE CULTIVO CELULAR

Country Status (23)

Country Link
US (2) US9353165B2 (es)
EP (1) EP2690110B1 (es)
JP (1) JP6096613B2 (es)
KR (1) KR101877352B1 (es)
CN (1) CN103570828B (es)
AR (1) AR091712A1 (es)
AU (1) AU2013203772B2 (es)
BR (1) BR102013016279B1 (es)
CA (1) CA2821384C (es)
CL (1) CL2013002024A1 (es)
ES (1) ES2628914T3 (es)
HU (1) HUE033582T2 (es)
IL (1) IL227409A (es)
MX (1) MX341733B (es)
MY (1) MY161395A (es)
NZ (1) NZ613080A (es)
PL (1) PL2690110T3 (es)
PT (1) PT2690110T (es)
RU (1) RU2617960C2 (es)
SG (1) SG196723A1 (es)
TW (1) TWI593702B (es)
UY (1) UY34915A (es)
ZA (1) ZA201305247B (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6783767B2 (ja) * 2014-12-22 2020-11-11 アレクシオン ファーマシューティカルズ インコーポレイテッドＡｌｅｘｉｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． 組換えタンパク質を精製する方法
AU2021225310A1 (en) * 2020-02-25 2022-10-06 Grifols Worldwide Operations Limited Method for obtaining Alpha-1 Proteinase Inhibitor
KR102284800B1 (ko) * 2020-12-30 2021-08-03 주식회사 하플사이언스 재조합 세포외 기질 단백질의 생산을 위한 동물세포 배양용 배지 조성물 및 이를 이용한 방법

Family Cites Families (27)

* Cited by examiner, † Cited by third party
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JPS60186290A (ja) * 1983-08-10 1985-09-21 チモ−ジエネテイツクス　インコ−ポレ−テツド アルフア−１−アンチトリプシンを細菌に表現させる方法
US4931373A (en) 1984-05-25 1990-06-05 Zymogenetics, Inc. Stable DNA constructs for expression of α-1 antitrypsin
US5134119A (en) 1990-10-16 1992-07-28 Lezdey John Treatment of inflammation using 358 substituted alpha-antitrypsin
US6284874B1 (en) 1994-06-17 2001-09-04 Alpha Therapeutic Corporation Process for separating α1-proteinase inhibitor from cohn fraction IV1 and IV4 paste
NZ267484A (en) * 1994-06-27 1997-12-19 Ioannis Stavridis Use of metal ion-porphyrin ring complexes in cigarette filters
US5616693A (en) 1996-07-01 1997-04-01 Alpha Therapeutic Corporation Process for seperating alpha-1-proteinase inhibitor from COHN IV1 +1V4 paste
US6093804A (en) 1998-09-24 2000-07-25 American National Red Cross Method for purification of alpha-1 proteinase inhibitor
US6462180B1 (en) 1999-11-24 2002-10-08 Bayer Corporation Method of preparing α-1 proteinase inhibitor
ES2543829T5 (es) 2000-12-14 2023-07-13 Grifols Therapeutics Inc Procedimiento para la preparación del inhibidor de proteinasa alfa 1
CZ20032208A3 (cs) * 2001-02-23 2004-01-14 Immunex Corporation Zvýšený výtěžek aktivních proteinů
US7777006B2 (en) * 2002-12-31 2010-08-17 Csl Behring L.L.C. Method for purification of alpha-1-antitrypsin
WO2005014655A2 (en) * 2003-08-08 2005-02-17 Fresenius Kabi Deutschland Gmbh Conjugates of hydroxyalkyl starch and a protein
BRPI0412835A (pt) * 2003-08-12 2006-09-26 Octapharma Ag processo para preparação de uma solução de alfa-1-antitripsina
WO2005027821A2 (en) 2003-09-22 2005-03-31 Kamada Ltd. Large scale preparation of alpha-1 proteinase inhibitor and use thereof
AU2004288854B2 (en) 2003-11-10 2009-10-01 Arriva-Pharmaceuticals, Inc. Dry recombinant human alpha 1-antitrypsin formulation
US7807435B2 (en) * 2005-08-11 2010-10-05 Baxter International Inc. Method for the purification of alpha-1 proteinase inhibitor (a1PI)
GB2446550B (en) 2005-11-28 2010-12-08 Proteomtech Inc Methods for production of recombinant alpha1-antitrypsin
GB0524432D0 (en) 2005-11-30 2006-01-11 Nhs Blood & Transplant Method
US8148502B2 (en) 2006-03-30 2012-04-03 Baxter International Inc. R-ALPHA1 antitrypsin anion exchange chromatography
CN101778860B (zh) * 2007-08-17 2013-07-10 Csl百灵有限公司 α-1-抗胰蛋白酶和载脂蛋白A-I的纯化方法
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PT2279265E (pt) * 2008-04-24 2014-12-09 Newsouth Innovations Pty Ltd Agrupamento de genes saxitoxina das cianobactérias e deteção de organismos cianotoxicos
NZ590257A (en) 2008-07-18 2012-08-31 Grifols Therapeutics Inc Method of preparing alpha-1 proteinase inhibitor
CA2756591C (en) 2009-04-23 2018-11-06 Crucell Holland B.V. Recombinant human alpha1-antitrypsin
JP2011205932A (ja) * 2010-03-29 2011-10-20 Sysmex Corp トロンビンの製造方法
US8772462B2 (en) * 2010-05-26 2014-07-08 Baxter International Inc. Removal of serine proteases by treatment with finely divided silicon dioxide
EP3412314A1 (en) * 2011-05-27 2018-12-12 Baxalta GmbH Therapeutic proteins conjugated to polysialic acid and methods of preparing same

Also Published As

Publication number Publication date
CL2013002024A1 (es) 2014-08-08
TW201406775A (zh) 2014-02-16
RU2013132132A (ru) 2015-01-20
NZ613080A (en) 2015-01-30
BR102013016279A2 (pt) 2015-07-14
UY34915A (es) 2014-02-28
PT2690110T (pt) 2017-06-27
CN103570828B (zh) 2017-12-15
KR20140013925A (ko) 2014-02-05
US9957315B2 (en) 2018-05-01
TWI593702B (zh) 2017-08-01
ZA201305247B (en) 2017-08-30
US9353165B2 (en) 2016-05-31
ES2628914T3 (es) 2017-08-04
MX341733B (es) 2016-08-31
MY161395A (en) 2017-04-14
BR102013016279B1 (pt) 2022-11-08
JP2014024842A (ja) 2014-02-06
HUE033582T2 (hu) 2017-12-28
US20140031532A1 (en) 2014-01-30
EP2690110A1 (en) 2014-01-29
RU2617960C2 (ru) 2017-04-28
IL227409A (en) 2017-02-28
CA2821384A1 (en) 2014-01-25
KR101877352B1 (ko) 2018-07-11
EP2690110B1 (en) 2017-05-03
AU2013203772A1 (en) 2014-02-13
US20160304583A1 (en) 2016-10-20
IL227409A0 (en) 2013-12-31
PL2690110T3 (pl) 2017-09-29
AU2013203772B2 (en) 2015-01-22
CA2821384C (en) 2019-03-26
JP6096613B2 (ja) 2017-03-15
MX2013008103A (es) 2014-03-27
CN103570828A (zh) 2014-02-12
SG196723A1 (en) 2014-02-13

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