AR091572A1 - Metodos y composiciones para calidad de forraje mejorada - Google Patents
Metodos y composiciones para calidad de forraje mejoradaInfo
- Publication number
- AR091572A1 AR091572A1 ARP130102260A AR091572A1 AR 091572 A1 AR091572 A1 AR 091572A1 AR P130102260 A ARP130102260 A AR P130102260A AR 091572 A1 AR091572 A1 AR 091572A1
- Authority
- AR
- Argentina
- Prior art keywords
- alfalfa
- alfalfa plant
- stem tissue
- dna molecule
- plant
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 239000000203 mixture Substances 0.000 title 1
- 241000219823 Medicago Species 0.000 abstract 11
- 102000053602 DNA Human genes 0.000 abstract 5
- 229920005610 lignin Polymers 0.000 abstract 4
- 108020004414 DNA Proteins 0.000 abstract 3
- 238000005259 measurement Methods 0.000 abstract 3
- 108020004511 Recombinant DNA Proteins 0.000 abstract 2
- 235000016709 nutrition Nutrition 0.000 abstract 2
- OBMBUODDCOAJQP-UHFFFAOYSA-N 2-chloro-4-phenylquinoline Chemical compound C=12C=CC=CC2=NC(Cl)=CC=1C1=CC=CC=C1 OBMBUODDCOAJQP-UHFFFAOYSA-N 0.000 abstract 1
- 239000002028 Biomass Substances 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 abstract 1
- 240000004658 Medicago sativa Species 0.000 abstract 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 239000003599 detergent Substances 0.000 abstract 1
- 235000019621 digestibility Nutrition 0.000 abstract 1
- 239000000835 fiber Substances 0.000 abstract 1
- 230000007935 neutral effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8255—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving lignin biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0098—Plants or trees
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Immunology (AREA)
- Virology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Mycology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Se provee un método para la selección de una planta de alfalfa con mejorado valor de alimentación y mayor flexibilidad, ya sea para incrementar la calidad de forraje proporcionada, ya sea para mejorar la biomasa disponible con anterioridad. Planta. Semilla y producto de alfalfa procesado. Reivindicación 1: Un método para la selección de una planta de alfalfa con componentes de valor nutricional mejorado, a partir de una población de plantas de alfalfa, donde dicha población de plantas de alfalfa comprende una molécula de ADN recombinado, y donde una porción de dicha molécula de ADN recombinado es homóloga o complementaria a SEC. ID. Nº 1 o SEC. ID. Nº 2, que comprende: (a) la medición de la concentración de lignina detergente ácida (ADL) en un tejido de tallo inferior de cada una de dichas plantas de alfalfa en dicha población de plantas de alfalfa, donde dicha concentración de ADL se reduce entre 12% y aproximadamente 31% en comparación con un tejido de tallo inferior de una planta de alfalfa que no comprende dicha molécula de ADN; (b) la medición de la concentración de guaiacil lignina (G lignina) en dicho tejido de tallo inferior, donde dicho contenido de G lignina es reducido alrededor de 25% o más en dicho tejido de tallo inferior, en comparación con un tejido de tallo inferior de una planta de alfalfa que no comprende dicha molécula de ADN; (c) la medición del componente de digestibilidad de fibra detergente neutra (NDFD) en dicho tejido de tallo inferior, donde dicho valor de NDFD está aumentado más de 18% en dicho tejido de tallo inferior en comparación con un tejido de tallo inferior de una planta de alfalfa que no comprende dicha molécula de ADN; (d) la selección de una planta de alfalfa que comprende los componentes de valor nutricional mejorado de las etapas (a) - (c); y (e) el cultivo de dicha planta de alfalfa seleccionada en la etapa (d).
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261664359P | 2012-06-26 | 2012-06-26 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR091572A1 true AR091572A1 (es) | 2015-02-11 |
Family
ID=49784009
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP130102260 AR091572A1 (es) | 2012-06-26 | 2013-06-26 | Metodos y composiciones para calidad de forraje mejorada |
ARP220100303A AR124881A2 (es) | 2012-06-26 | 2022-02-15 | Métodos y composiciones para calidad de forraje mejorada |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP220100303A AR124881A2 (es) | 2012-06-26 | 2022-02-15 | Métodos y composiciones para calidad de forraje mejorada |
Country Status (13)
Country | Link |
---|---|
US (2) | US9771597B2 (es) |
EP (1) | EP2864486B1 (es) |
JP (2) | JP2015527878A (es) |
CN (1) | CN104603275A (es) |
AR (2) | AR091572A1 (es) |
AU (1) | AU2013280398B2 (es) |
BR (2) | BR122019026534B1 (es) |
CA (1) | CA2877873C (es) |
MX (1) | MX362498B (es) |
NZ (1) | NZ629197A (es) |
PL (1) | PL2864486T3 (es) |
WO (1) | WO2014004683A2 (es) |
ZA (1) | ZA201408419B (es) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9854778B2 (en) | 2011-06-30 | 2018-01-02 | Monsanto Technology Llc | Alfalfa plant and seed corresponding to transgenic event KK 179-2 and methods for detection thereof |
CA2840630C (en) | 2011-06-30 | 2021-11-30 | Monsanto Technology Llc | Alfalfa plant and seed corresponding to transgenic event kk 179-2 and methods for detection thereof |
BR122019026534B1 (pt) * | 2012-06-26 | 2022-07-19 | Monsanto Technology Llc | Método para selecionar uma planta alfafa com componentes de valor alimentar melhorados de uma população de plantas alfafa |
CA2962242A1 (en) | 2014-09-29 | 2016-04-07 | Agrigenetics, Inc. | Low lignin non-transgenic alfalfa varieties and methods for producing the same |
KR102198787B1 (ko) * | 2019-09-05 | 2021-01-05 | 한국과학기술연구원 | 변이된 comt 유전자를 포함하는 사료용 작물 및 이의 용도 |
Family Cites Families (31)
Publication number | Priority date | Publication date | Assignee | Title |
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ES2039474T3 (es) | 1986-12-05 | 1993-10-01 | Ciba-Geigy Ag | Procedimineto mejorado para la transformacion de protoplastos vegetales. |
US5015580A (en) | 1987-07-29 | 1991-05-14 | Agracetus | Particle-mediated transformation of soybean plants and lines |
US5416011A (en) | 1988-07-22 | 1995-05-16 | Monsanto Company | Method for soybean transformation and regeneration |
GB8829298D0 (en) | 1988-12-15 | 1989-01-25 | Ici Plc | Improved fodder crops |
US7705215B1 (en) | 1990-04-17 | 2010-04-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
US5550318A (en) | 1990-04-17 | 1996-08-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
US5484956A (en) | 1990-01-22 | 1996-01-16 | Dekalb Genetics Corporation | Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin |
JP3209744B2 (ja) | 1990-01-22 | 2001-09-17 | デカルブ・ジェネティクス・コーポレーション | 結実能力のある遺伝子変換コーン |
US5187092A (en) | 1990-03-22 | 1993-02-16 | Institute Of Paper Science And Technology, Inc. | Somatic embryogenesis in gymnosperms |
US6403865B1 (en) | 1990-08-24 | 2002-06-11 | Syngenta Investment Corp. | Method of producing transgenic maize using direct transformation of commercially important genotypes |
US5384253A (en) | 1990-12-28 | 1995-01-24 | Dekalb Genetics Corporation | Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes |
US5591616A (en) | 1992-07-07 | 1997-01-07 | Japan Tobacco, Inc. | Method for transforming monocotyledons |
HUT70467A (en) | 1992-07-27 | 1995-10-30 | Pioneer Hi Bred Int | An improved method of agrobactenium-mediated transformation of cultvred soyhean cells |
US5635055A (en) | 1994-07-19 | 1997-06-03 | Exxon Research & Engineering Company | Membrane process for increasing conversion of catalytic cracking or thermal cracking units (law011) |
US5981840A (en) | 1997-01-24 | 1999-11-09 | Pioneer Hi-Bred International, Inc. | Methods for agrobacterium-mediated transformation |
US6455762B1 (en) | 1997-11-12 | 2002-09-24 | Board Of Control Of Michigan Technological University | Methods of modifying lignin in plants by transformation with a 4-coumarate coenzyme a ligase nucleic acid |
JP2002534129A (ja) | 1999-01-14 | 2002-10-15 | モンサント テクノロジー エルエルシー | ダイズ形質転換方法 |
US20040049802A1 (en) | 2001-03-23 | 2004-03-11 | Dixon Richard A. | Method for modifying lignin composition and increasing in vivo digestibility of forages |
AU4773101A (en) | 2000-03-24 | 2001-10-08 | Samuel Roberts Noble Found Inc | Method for modifying lignin composition and increasing in vivo digestibility of forages |
AUPQ815400A0 (en) | 2000-06-14 | 2000-07-06 | Dairy Research And Development Corporation | Modification of lignin biosynthesis |
ATE553202T1 (de) | 2003-01-31 | 2012-04-15 | Monsanto Technology Llc | Glyphosat-tolerante luzernepflanzen und verfahren zu deren detektion |
WO2005068625A1 (en) | 2004-01-05 | 2005-07-28 | The Regents Of The University Of California | Plants transformed for elevated levels of gallic acid and methods of producing said plants |
US7855323B2 (en) | 2004-02-10 | 2010-12-21 | Monsanto Technology Llc | Recombinant DNA for gene suppression |
EP1771568A2 (en) * | 2004-07-24 | 2007-04-11 | The Samuel Roberts Noble Foundation, Inc. | Modification of lignin biosynthesis |
US20090199308A1 (en) * | 2005-08-30 | 2009-08-06 | Kimberly Zobrist Duff | Transgenic plants with enhanced agronomic traits |
US7652195B2 (en) | 2005-10-04 | 2010-01-26 | Pioneer Hi-Bred International, Inc. | Alfalfa variety 53V52 |
US20110229625A1 (en) | 2010-02-17 | 2011-09-22 | William Ralph Hiatt | Transgenic Forage Crops with Enhanced Nutrition |
AR085759A1 (es) * | 2011-04-21 | 2013-10-23 | Basf Plant Science Co Gmbh | Metodos para modificar la biosintesis de la lignina y mejorar la digestibilidad |
US9854778B2 (en) | 2011-06-30 | 2018-01-02 | Monsanto Technology Llc | Alfalfa plant and seed corresponding to transgenic event KK 179-2 and methods for detection thereof |
CA2840630C (en) * | 2011-06-30 | 2021-11-30 | Monsanto Technology Llc | Alfalfa plant and seed corresponding to transgenic event kk 179-2 and methods for detection thereof |
BR122019026534B1 (pt) * | 2012-06-26 | 2022-07-19 | Monsanto Technology Llc | Método para selecionar uma planta alfafa com componentes de valor alimentar melhorados de uma população de plantas alfafa |
-
2013
- 2013-06-26 BR BR122019026534-8A patent/BR122019026534B1/pt active IP Right Grant
- 2013-06-26 EP EP13808555.0A patent/EP2864486B1/en active Active
- 2013-06-26 WO PCT/US2013/047911 patent/WO2014004683A2/en active Application Filing
- 2013-06-26 AR ARP130102260 patent/AR091572A1/es not_active Application Discontinuation
- 2013-06-26 BR BR112014032343A patent/BR112014032343A2/pt not_active Application Discontinuation
- 2013-06-26 NZ NZ629197A patent/NZ629197A/en not_active IP Right Cessation
- 2013-06-26 AU AU2013280398A patent/AU2013280398B2/en active Active
- 2013-06-26 CA CA2877873A patent/CA2877873C/en active Active
- 2013-06-26 MX MX2014015422A patent/MX362498B/es active IP Right Grant
- 2013-06-26 JP JP2015520467A patent/JP2015527878A/ja active Pending
- 2013-06-26 US US14/405,781 patent/US9771597B2/en active Active
- 2013-06-26 PL PL13808555T patent/PL2864486T3/pl unknown
- 2013-06-26 CN CN201380032568.5A patent/CN104603275A/zh active Pending
-
2014
- 2014-11-17 ZA ZA2014/08419A patent/ZA201408419B/en unknown
-
2017
- 2017-09-19 US US15/709,297 patent/US10704053B2/en active Active
-
2018
- 2018-01-16 JP JP2018004739A patent/JP2018108079A/ja active Pending
-
2022
- 2022-02-15 AR ARP220100303A patent/AR124881A2/es unknown
Also Published As
Publication number | Publication date |
---|---|
CN104603275A (zh) | 2015-05-06 |
NZ629197A (en) | 2016-07-29 |
BR122019026534B1 (pt) | 2022-07-19 |
US20180080038A1 (en) | 2018-03-22 |
WO2014004683A2 (en) | 2014-01-03 |
EP2864486B1 (en) | 2018-09-26 |
CA2877873A1 (en) | 2014-01-03 |
US9771597B2 (en) | 2017-09-26 |
CA2877873C (en) | 2020-11-24 |
ZA201408419B (en) | 2015-12-23 |
JP2018108079A (ja) | 2018-07-12 |
MX2014015422A (es) | 2015-04-09 |
JP2015527878A (ja) | 2015-09-24 |
AU2013280398A1 (en) | 2014-12-04 |
EP2864486A2 (en) | 2015-04-29 |
PL2864486T3 (pl) | 2019-05-31 |
BR112014032343A2 (pt) | 2017-08-01 |
WO2014004683A3 (en) | 2014-04-24 |
US20150135370A1 (en) | 2015-05-14 |
AR124881A2 (es) | 2023-05-17 |
US10704053B2 (en) | 2020-07-07 |
MX362498B (es) | 2019-01-21 |
AU2013280398B2 (en) | 2018-07-12 |
EP2864486A4 (en) | 2016-03-02 |
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