AR091572A1 - Metodos y composiciones para calidad de forraje mejorada - Google Patents

Metodos y composiciones para calidad de forraje mejorada

Info

Publication number
AR091572A1
AR091572A1 ARP130102260A AR091572A1 AR 091572 A1 AR091572 A1 AR 091572A1 AR P130102260 A ARP130102260 A AR P130102260A AR 091572 A1 AR091572 A1 AR 091572A1
Authority
AR
Argentina
Prior art keywords
alfalfa
alfalfa plant
stem tissue
dna molecule
plant
Prior art date
Application number
Other languages
English (en)
Original Assignee
Monsanto Technology Llc
Forage Genetics International Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc, Forage Genetics International Llc filed Critical Monsanto Technology Llc
Publication of AR091572A1 publication Critical patent/AR091572A1/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8255Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving lignin biosynthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/0098Plants or trees
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/13Plant traits
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Mycology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Fodder In General (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Se provee un método para la selección de una planta de alfalfa con mejorado valor de alimentación y mayor flexibilidad, ya sea para incrementar la calidad de forraje proporcionada, ya sea para mejorar la biomasa disponible con anterioridad. Planta. Semilla y producto de alfalfa procesado. Reivindicación 1: Un método para la selección de una planta de alfalfa con componentes de valor nutricional mejorado, a partir de una población de plantas de alfalfa, donde dicha población de plantas de alfalfa comprende una molécula de ADN recombinado, y donde una porción de dicha molécula de ADN recombinado es homóloga o complementaria a SEC. ID. Nº 1 o SEC. ID. Nº 2, que comprende: (a) la medición de la concentración de lignina detergente ácida (ADL) en un tejido de tallo inferior de cada una de dichas plantas de alfalfa en dicha población de plantas de alfalfa, donde dicha concentración de ADL se reduce entre 12% y aproximadamente 31% en comparación con un tejido de tallo inferior de una planta de alfalfa que no comprende dicha molécula de ADN; (b) la medición de la concentración de guaiacil lignina (G lignina) en dicho tejido de tallo inferior, donde dicho contenido de G lignina es reducido alrededor de 25% o más en dicho tejido de tallo inferior, en comparación con un tejido de tallo inferior de una planta de alfalfa que no comprende dicha molécula de ADN; (c) la medición del componente de digestibilidad de fibra detergente neutra (NDFD) en dicho tejido de tallo inferior, donde dicho valor de NDFD está aumentado más de 18% en dicho tejido de tallo inferior en comparación con un tejido de tallo inferior de una planta de alfalfa que no comprende dicha molécula de ADN; (d) la selección de una planta de alfalfa que comprende los componentes de valor nutricional mejorado de las etapas (a) - (c); y (e) el cultivo de dicha planta de alfalfa seleccionada en la etapa (d).
ARP130102260 2012-06-26 2013-06-26 Metodos y composiciones para calidad de forraje mejorada AR091572A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261664359P 2012-06-26 2012-06-26

Publications (1)

Publication Number Publication Date
AR091572A1 true AR091572A1 (es) 2015-02-11

Family

ID=49784009

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP130102260 AR091572A1 (es) 2012-06-26 2013-06-26 Metodos y composiciones para calidad de forraje mejorada
ARP220100303A AR124881A2 (es) 2012-06-26 2022-02-15 Métodos y composiciones para calidad de forraje mejorada

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP220100303A AR124881A2 (es) 2012-06-26 2022-02-15 Métodos y composiciones para calidad de forraje mejorada

Country Status (13)

Country Link
US (2) US9771597B2 (es)
EP (1) EP2864486B1 (es)
JP (2) JP2015527878A (es)
CN (1) CN104603275A (es)
AR (2) AR091572A1 (es)
AU (1) AU2013280398B2 (es)
BR (2) BR122019026534B1 (es)
CA (1) CA2877873C (es)
MX (1) MX362498B (es)
NZ (1) NZ629197A (es)
PL (1) PL2864486T3 (es)
WO (1) WO2014004683A2 (es)
ZA (1) ZA201408419B (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9854778B2 (en) 2011-06-30 2018-01-02 Monsanto Technology Llc Alfalfa plant and seed corresponding to transgenic event KK 179-2 and methods for detection thereof
CA2840630C (en) 2011-06-30 2021-11-30 Monsanto Technology Llc Alfalfa plant and seed corresponding to transgenic event kk 179-2 and methods for detection thereof
BR122019026534B1 (pt) * 2012-06-26 2022-07-19 Monsanto Technology Llc Método para selecionar uma planta alfafa com componentes de valor alimentar melhorados de uma população de plantas alfafa
CA2962242A1 (en) 2014-09-29 2016-04-07 Agrigenetics, Inc. Low lignin non-transgenic alfalfa varieties and methods for producing the same
KR102198787B1 (ko) * 2019-09-05 2021-01-05 한국과학기술연구원 변이된 comt 유전자를 포함하는 사료용 작물 및 이의 용도

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Also Published As

Publication number Publication date
CN104603275A (zh) 2015-05-06
NZ629197A (en) 2016-07-29
BR122019026534B1 (pt) 2022-07-19
US20180080038A1 (en) 2018-03-22
WO2014004683A2 (en) 2014-01-03
EP2864486B1 (en) 2018-09-26
CA2877873A1 (en) 2014-01-03
US9771597B2 (en) 2017-09-26
CA2877873C (en) 2020-11-24
ZA201408419B (en) 2015-12-23
JP2018108079A (ja) 2018-07-12
MX2014015422A (es) 2015-04-09
JP2015527878A (ja) 2015-09-24
AU2013280398A1 (en) 2014-12-04
EP2864486A2 (en) 2015-04-29
PL2864486T3 (pl) 2019-05-31
BR112014032343A2 (pt) 2017-08-01
WO2014004683A3 (en) 2014-04-24
US20150135370A1 (en) 2015-05-14
AR124881A2 (es) 2023-05-17
US10704053B2 (en) 2020-07-07
MX362498B (es) 2019-01-21
AU2013280398B2 (en) 2018-07-12
EP2864486A4 (en) 2016-03-02

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