AR086674A1 - Metodo para la generacion de una linea celular de planta monoclonal - Google Patents
Metodo para la generacion de una linea celular de planta monoclonalInfo
- Publication number
- AR086674A1 AR086674A1 ARP120101980A ARP120101980A AR086674A1 AR 086674 A1 AR086674 A1 AR 086674A1 AR P120101980 A ARP120101980 A AR P120101980A AR P120101980 A ARP120101980 A AR P120101980A AR 086674 A1 AR086674 A1 AR 086674A1
- Authority
- AR
- Argentina
- Prior art keywords
- microcolony
- protoplasts
- cell line
- plant cells
- plant cell
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
La presente provee un método para la generación de una línea celular de planta monoclonal a partir de una población heteróloga de células de plantas, que comprende las siguientes etapas: (a) la provisión de una población heteróloga de células de plantas; (b) la preparación de protoplastos a partir de dicha población heteróloga de células de plantas; (c) la separación de protoplastos individuales, sometiendo la preparación de protoplastos a la selección de citometría de flujo; (d) la regeneración de un protoplasto transformado individual separado hasta la formación de una microcolonia, por medio del cocultivo en presencia de material celular de alimentación; (e) la remoción de la microcolonia del material celular de alimentación, y el cultivo de la microcolonia hasta la formación de una línea celular de planta monoclonal.Reivindicación 1: Un método para la generación de una línea celular de planta monoclonal a partir de una población heteróloga de células de plantas, que comprende las siguientes etapas: (a) la provisión de una población heteróloga de células de plantas; (b) la preparación de protoplastos a partir de dicha población heteróloga de células de plantas; (c) la separación de protoplastos individuales, sometiendo la preparación de protoplastos a la selección de citometría de flujo; (d) la regeneración de un protoplasto transformado individual separado hasta la formación de una microcolonia, por medio del cocultivo en presencia de material celular de alimentación; (e) la remoción de la microcolonia del material celular de alimentación, y el cultivo de la microcolonia hasta la formación de una línea celular de planta monoclonal.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP2011/002785 WO2012167803A1 (en) | 2011-06-07 | 2011-06-07 | Method for the generation of a monoclonal plant cell line |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR086674A1 true AR086674A1 (es) | 2014-01-15 |
Family
ID=44529878
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP120101980A AR086674A1 (es) | 2011-06-07 | 2012-06-05 | Metodo para la generacion de una linea celular de planta monoclonal |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US10480001B2 (es) |
| EP (1) | EP2717676B1 (es) |
| JP (1) | JP5933700B2 (es) |
| KR (1) | KR101933552B1 (es) |
| CN (1) | CN103596423B (es) |
| AR (1) | AR086674A1 (es) |
| AU (1) | AU2011370336B2 (es) |
| BR (1) | BR112013031244B1 (es) |
| CA (1) | CA2835253C (es) |
| IL (1) | IL229168A0 (es) |
| RU (1) | RU2573927C2 (es) |
| TW (1) | TWI608795B (es) |
| UA (1) | UA112546C2 (es) |
| WO (1) | WO2012167803A1 (es) |
| ZA (1) | ZA201309126B (es) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140075593A1 (en) * | 2012-09-07 | 2014-03-13 | Dow Agrosciences Llc | Fluorescence activated cell sorting (facs) enrichment to generate plants |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4300310A (en) | 1980-07-28 | 1981-11-17 | The Board Of Regents Of The University Of Nebraska | Identification and sorting of plant heterokaryons |
| JPH03143390A (ja) * | 1989-10-27 | 1991-06-18 | Naasarii Technol:Kk | 植物細胞培養法 |
| JPH11505422A (ja) * | 1995-05-19 | 1999-05-21 | フィテラ インク． | 植物細胞培養物および植物組織培養物の処理方法 |
| WO2001088168A2 (en) * | 2000-05-12 | 2001-11-22 | Odyssey Pharmaceuticals, Inc. | Mapping molecular interactions in plants with protein fragments complementation assays |
| NZ529597A (en) * | 2001-05-30 | 2005-12-23 | Chromos Molecular Systems Inc | Plant artificial chromosomes, uses thereof and methods of preparing plant artificial chromosomes |
| US8247655B2 (en) | 2006-09-13 | 2012-08-21 | Syngenta Participations Ag | Rucola plants with cytoplasmic male sterility (CMS) |
| CN101402938B (zh) * | 2008-11-17 | 2010-10-13 | 中国科学院植物研究所 | 一种制备原生质体的方法 |
-
2011
- 2011-06-07 BR BR112013031244-0A patent/BR112013031244B1/pt not_active IP Right Cessation
- 2011-06-07 CN CN201180071503.2A patent/CN103596423B/zh not_active Expired - Fee Related
- 2011-06-07 US US14/115,629 patent/US10480001B2/en not_active Expired - Fee Related
- 2011-06-07 RU RU2013157526/10A patent/RU2573927C2/ru active IP Right Revival
- 2011-06-07 JP JP2014513915A patent/JP5933700B2/ja not_active Expired - Fee Related
- 2011-06-07 WO PCT/EP2011/002785 patent/WO2012167803A1/en active Application Filing
- 2011-06-07 EP EP11729369.6A patent/EP2717676B1/en not_active Not-in-force
- 2011-06-07 AU AU2011370336A patent/AU2011370336B2/en not_active Ceased
- 2011-06-07 KR KR1020137032519A patent/KR101933552B1/ko active IP Right Grant
- 2011-06-07 CA CA2835253A patent/CA2835253C/en not_active Expired - Fee Related
- 2011-07-06 UA UAA201315360A patent/UA112546C2/uk unknown
-
2012
- 2012-06-05 AR ARP120101980A patent/AR086674A1/es unknown
- 2012-06-06 TW TW101120200A patent/TWI608795B/zh not_active IP Right Cessation
-
2013
- 2013-10-31 IL IL229168A patent/IL229168A0/en active IP Right Grant
- 2013-12-04 ZA ZA2013/09126A patent/ZA201309126B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| UA112546C2 (uk) | 2016-09-26 |
| CN103596423A (zh) | 2014-02-19 |
| AU2011370336A1 (en) | 2013-12-12 |
| EP2717676A1 (en) | 2014-04-16 |
| JP5933700B2 (ja) | 2016-06-15 |
| KR20140048880A (ko) | 2014-04-24 |
| BR112013031244B1 (pt) | 2019-10-29 |
| US10480001B2 (en) | 2019-11-19 |
| RU2013157526A (ru) | 2015-07-20 |
| JP2014516553A (ja) | 2014-07-17 |
| US20140053300A1 (en) | 2014-02-20 |
| BR112013031244A2 (pt) | 2017-06-27 |
| TWI608795B (zh) | 2017-12-21 |
| WO2012167803A1 (en) | 2012-12-13 |
| CA2835253C (en) | 2018-06-26 |
| IL229168A0 (en) | 2013-12-31 |
| AU2011370336B2 (en) | 2017-07-06 |
| CN103596423B (zh) | 2017-05-24 |
| CA2835253A1 (en) | 2012-12-13 |
| EP2717676B1 (en) | 2019-01-02 |
| RU2573927C2 (ru) | 2016-01-27 |
| ZA201309126B (en) | 2014-09-25 |
| TW201300014A (zh) | 2013-01-01 |
| KR101933552B1 (ko) | 2018-12-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |