AR072563A1 - Un procedimiento para obtener insulina aspartica que utiliza una cepa de levaduras de pichia pastoris - Google Patents
Un procedimiento para obtener insulina aspartica que utiliza una cepa de levaduras de pichia pastorisInfo
- Publication number
- AR072563A1 AR072563A1 ARP090102686A ARP090102686A AR072563A1 AR 072563 A1 AR072563 A1 AR 072563A1 AR P090102686 A ARP090102686 A AR P090102686A AR P090102686 A ARP090102686 A AR P090102686A AR 072563 A1 AR072563 A1 AR 072563A1
- Authority
- AR
- Argentina
- Prior art keywords
- aspartic insulin
- precursor
- insulin
- aspartic
- dna construct
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Endocrinology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Diabetes (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
Además una construccion de ADN para su uso en el procedimiento para obtener insulina aspártica. Reivindicacion 1: Un procedimiento para obtener insulina aspártica caracterizado porque comprende: i) transformar una cepa de levaduras de Pichia pastoris con al menos dos vectores de expresion que posean marcadores de seleccion distintos entre sí y en donde cada vector comprende una construccion de ADN que codifica para an precursor de insulina aspártica de la formula B (1-30) -X1-Y-X2-A (1-21); ii) seleccionar entre las cepas transformadas de acuerdo con la etapa anterior, aquellas cepas recombinantes que sean MUT S y que contengan más de 5 copias de la construccion de ADN integradas al genoma de la levadura; iii) seleccionar entre las cepas recombinantes de la etapa anterior aquella que posea un mayor numero de copias y que tenga un mayor nivel de expresion del precursor de insulina aspártica; iv) fermentar dicha cepa recombinante; v) capturar el precursor de la insulina aspártica del medio de cultivo mediante técnicas cromatográficas, vi) digerir dicho precursor enzimáticamente en condiciones adecuadas corno para que al menos el 70% del precursor de insulina aspártica se convierta en insulina aspártica madura; y vii) purificar la insulina aspártica obtenida a partir de dicha digestion mediante técnicas cromatográficas convencionales.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ARP090102686A AR072563A1 (es) | 2009-07-15 | 2009-07-15 | Un procedimiento para obtener insulina aspartica que utiliza una cepa de levaduras de pichia pastoris |
| US12/836,864 US8691530B2 (en) | 2009-07-15 | 2010-07-15 | Process for obtaining aspart insulin using a Pichia pastoris yeast strain |
| EA201001021A EA201001021A8 (ru) | 2009-07-15 | 2010-07-15 | Способ получения инсулина аспарт при помощи штамма дрожжей pichia pastoris |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ARP090102686A AR072563A1 (es) | 2009-07-15 | 2009-07-15 | Un procedimiento para obtener insulina aspartica que utiliza una cepa de levaduras de pichia pastoris |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR072563A1 true AR072563A1 (es) | 2010-09-08 |
Family
ID=42829212
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP090102686A AR072563A1 (es) | 2009-07-15 | 2009-07-15 | Un procedimiento para obtener insulina aspartica que utiliza una cepa de levaduras de pichia pastoris |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US8691530B2 (es) |
| AR (1) | AR072563A1 (es) |
| EA (1) | EA201001021A8 (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2458989C1 (ru) * | 2008-08-07 | 2012-08-20 | Байокон Лимитид | Способ получения аналогов инсулина из их соответствующих предшественников (варианты) |
| EP3268384B1 (en) | 2015-03-10 | 2021-11-03 | Merck Sharp & Dohme Corp. | Process for preparing recombinant insulin using microfiltration |
| RU2697218C1 (ru) * | 2018-07-11 | 2019-08-13 | федеральное государственное автономное образовательное учреждение высшего образования "Московский физико-технический институт (национальный исследовательский университет)" | Использование сигнальных пептидов митохондриальной локализации для увеличения уровня гетерологической экспрессии белков в P.pastoris и S.cerevisiae |
| CN113226284A (zh) * | 2018-09-25 | 2021-08-06 | 美药星制药股份有限公司 | 高度纯化的重组人胰岛素(rhi)api及其生产方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK129385A (da) * | 1985-03-22 | 1986-09-23 | Novo Industri As | Peptider og fremstilling deraf |
-
2009
- 2009-07-15 AR ARP090102686A patent/AR072563A1/es unknown
-
2010
- 2010-07-15 EA EA201001021A patent/EA201001021A8/ru not_active IP Right Cessation
- 2010-07-15 US US12/836,864 patent/US8691530B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EA022946B1 (ru) | 2016-03-31 |
| EA201001021A8 (ru) | 2016-03-31 |
| US8691530B2 (en) | 2014-04-08 |
| US20110117600A1 (en) | 2011-05-19 |
| EA201001021A1 (ru) | 2011-04-29 |
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| FB | Suspension of granting procedure |