RU2013126413A - Понижающая регуляция экспрессии гена с помощью искусственных микрорнк - Google Patents
Понижающая регуляция экспрессии гена с помощью искусственных микрорнк Download PDFInfo
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- RU2013126413A RU2013126413A RU2013126413/10A RU2013126413A RU2013126413A RU 2013126413 A RU2013126413 A RU 2013126413A RU 2013126413/10 A RU2013126413/10 A RU 2013126413/10A RU 2013126413 A RU2013126413 A RU 2013126413A RU 2013126413 A RU2013126413 A RU 2013126413A
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
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Abstract
1. Выделенный фрагмент нуклеиновой кислоты для понижения экспрессии последовательности-мишени, по существу являющейся дезоксирибонуклеотидной последовательностью, представленной в SEQ ID NO:15, где (i) нуклеотиды 83-103 в SEQ ID NO:15 замещены первой вариабельной нуклеотидной субпоследовательностью, размер которой варьирует от 19 до 24 нуклеотидов в зависимости от последовательности-мишени, экспрессия которой подлежит понижению, (ii) нуклеотиды 172-192 в SEQ ID NO:15 замещены второй вариабельной нуклеотидной субпоследовательностью, размер которой варьирует от 19 до 24 нуклеотидов, причем указанная вторая вариабельная нуклеотидная субпоследовательность способна гибридизоваться с первой вариабельной субпоследовательностью предшественника миРНК, и (iii) предшественник миРНК, полученный указанным выделенным фрагментом нуклеиновой кислоты, имеет туже стеблевую структуру, что и предшественник миРНК, полученный эндогенной последовательностью SEQ ID NO:15.2. Рекомбинантная конструкция для понижения экспрессии последовательности-мишени, включающая выделенный фрагмент нуклеиновой кислоты по п. 1, функционально связанный, по меньшей мере, с одной регуляторной последовательностью.3. Клетка маиса для понижения экспрессии последовательности-мишени, включающая рекомбинантную конструкцию по п. 2.4. Способ отбора клеток, имеющих пониженный уровень экспрессии, включающий:(a) трансформацию, по меньшей мере, одной клетки маисарекомбинантная конструкция по п. 2; и(b) отбор той трансформированной клетки (клеток) маиса, у которых уровень экспрессии той последовательности-мишени понижен по сравнению с уровнем экспрессии гена-мишени в клетке маиса диког
Claims (4)
1. Выделенный фрагмент нуклеиновой кислоты для понижения экспрессии последовательности-мишени, по существу являющейся дезоксирибонуклеотидной последовательностью, представленной в SEQ ID NO:15, где (i) нуклеотиды 83-103 в SEQ ID NO:15 замещены первой вариабельной нуклеотидной субпоследовательностью, размер которой варьирует от 19 до 24 нуклеотидов в зависимости от последовательности-мишени, экспрессия которой подлежит понижению, (ii) нуклеотиды 172-192 в SEQ ID NO:15 замещены второй вариабельной нуклеотидной субпоследовательностью, размер которой варьирует от 19 до 24 нуклеотидов, причем указанная вторая вариабельная нуклеотидная субпоследовательность способна гибридизоваться с первой вариабельной субпоследовательностью предшественника миРНК, и (iii) предшественник миРНК, полученный указанным выделенным фрагментом нуклеиновой кислоты, имеет туже стеблевую структуру, что и предшественник миРНК, полученный эндогенной последовательностью SEQ ID NO:15.
2. Рекомбинантная конструкция для понижения экспрессии последовательности-мишени, включающая выделенный фрагмент нуклеиновой кислоты по п. 1, функционально связанный, по меньшей мере, с одной регуляторной последовательностью.
3. Клетка маиса для понижения экспрессии последовательности-мишени, включающая рекомбинантную конструкцию по п. 2.
4. Способ отбора клеток, имеющих пониженный уровень экспрессии, включающий:
(a) трансформацию, по меньшей мере, одной клетки маиса
рекомбинантная конструкция по п. 2; и
(b) отбор той трансформированной клетки (клеток) маиса, у которых уровень экспрессии той последовательности-мишени понижен по сравнению с уровнем экспрессии гена-мишени в клетке маиса дикого типа.
Applications Claiming Priority (2)
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US1451007P | 2007-12-18 | 2007-12-18 | |
US61/014,510 | 2007-12-18 |
Related Parent Applications (1)
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RU2010129428/10A Division RU2492239C2 (ru) | 2007-12-18 | 2008-12-17 | Понижающая регуляция экспрессии гена с помощью искусственных микрорнк |
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RU2013126413A true RU2013126413A (ru) | 2014-12-20 |
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RU2010129428/10A RU2492239C2 (ru) | 2007-12-18 | 2008-12-17 | Понижающая регуляция экспрессии гена с помощью искусственных микрорнк |
RU2013126413/10A RU2013126413A (ru) | 2007-12-18 | 2013-06-07 | Понижающая регуляция экспрессии гена с помощью искусственных микрорнк |
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RU2010129428/10A RU2492239C2 (ru) | 2007-12-18 | 2008-12-17 | Понижающая регуляция экспрессии гена с помощью искусственных микрорнк |
Country Status (9)
Country | Link |
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US (5) | US8115055B2 (ru) |
EP (4) | EP2222858B1 (ru) |
CN (2) | CN101903525B (ru) |
BR (1) | BRPI0819495A2 (ru) |
CA (5) | CA2895048C (ru) |
RU (2) | RU2492239C2 (ru) |
UA (1) | UA101823C2 (ru) |
WO (1) | WO2009079548A2 (ru) |
ZA (1) | ZA201004123B (ru) |
Families Citing this family (19)
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US20160024518A1 (en) | 2013-03-13 | 2016-01-28 | Brian McGonigle | Production of small interfering rnas in planta |
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WO2022204466A1 (en) | 2021-03-26 | 2022-09-29 | Flagship Pioneering Innovations Vii, Llc | Production of circular polyribonucleotides in a prokaryotic system |
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- 2008-12-17 US US12/747,606 patent/US20110033937A1/en not_active Abandoned
- 2008-12-17 CA CA2895049A patent/CA2895049C/en not_active Expired - Fee Related
- 2008-12-17 EP EP12197452.1A patent/EP2573182B1/en not_active Not-in-force
-
2010
- 2010-06-09 ZA ZA2010/04123A patent/ZA201004123B/en unknown
-
2012
- 2012-01-11 US US13/348,225 patent/US8415526B2/en active Active
- 2012-01-11 US US13/348,215 patent/US8273951B2/en active Active
- 2012-08-22 US US13/591,549 patent/US8536405B2/en active Active
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2013
- 2013-06-07 RU RU2013126413/10A patent/RU2013126413A/ru not_active Application Discontinuation
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