UA101823C2 - Даун-регуляция экспрессии гена с помощью искусственных микрорнк - Google Patents

Даун-регуляция экспрессии гена с помощью искусственных микрорнк Download PDF

Info

Publication number
UA101823C2
UA101823C2 UAA201009061A UAA201009061A UA101823C2 UA 101823 C2 UA101823 C2 UA 101823C2 UA A201009061 A UAA201009061 A UA A201009061A UA A201009061 A UAA201009061 A UA A201009061A UA 101823 C2 UA101823 C2 UA 101823C2
Authority
UA
Ukraine
Prior art keywords
mirna
dna
artificial
sequence
expression
Prior art date
Application number
UAA201009061A
Other languages
English (en)
Ukrainian (uk)
Inventor
Брайан МАКГОНИГЛ
Original Assignee
Э. И. Дю Пон Де Немур Энд Компани
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Э. И. Дю Пон Де Немур Энд Компани filed Critical Э. И. Дю Пон Де Немур Энд Компани
Publication of UA101823C2 publication Critical patent/UA101823C2/ru

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Virology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Изобретение относится к выделенному фрагменту нуклеиновой кислоты, который включает миРНК-предшественник, искусственные миРНК и их применение в Даун-регулировании экспрессии гена.
UAA201009061A 2007-12-18 2008-12-17 Даун-регуляция экспрессии гена с помощью искусственных микрорнк UA101823C2 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1451007P 2007-12-18 2007-12-18
PCT/US2008/087136 WO2009079548A2 (en) 2007-12-18 2008-12-17 Down-regulation of gene expression using artificial micrornas

Publications (1)

Publication Number Publication Date
UA101823C2 true UA101823C2 (ru) 2013-05-13

Family

ID=40428299

Family Applications (1)

Application Number Title Priority Date Filing Date
UAA201009061A UA101823C2 (ru) 2007-12-18 2008-12-17 Даун-регуляция экспрессии гена с помощью искусственных микрорнк

Country Status (9)

Country Link
US (5) US8115055B2 (ru)
EP (4) EP2573180B1 (ru)
CN (2) CN103898114B (ru)
BR (1) BRPI0819495A2 (ru)
CA (5) CA2895049C (ru)
RU (2) RU2492239C2 (ru)
UA (1) UA101823C2 (ru)
WO (1) WO2009079548A2 (ru)
ZA (1) ZA201004123B (ru)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9062317B2 (en) 2011-05-09 2015-06-23 E I Du Pont De Nemours And Company Methods and compositions for silencing gene families using artificial microRNAs
CA2850390A1 (en) 2011-10-28 2013-05-02 E. I. Du Pont De Nemours And Company Methods and compositions for silencing genes using artificial micrornas
US20140107361A1 (en) * 2012-01-23 2014-04-17 Linnaeus Plant Sciences Inc. Modifying the fatty acid profile of camelina sativa oil
EP2807258B1 (en) 2012-01-23 2017-04-26 E. I. du Pont de Nemours and Company Down-regulation of gene expression using artificial micrornas for silencing fatty acid biosynthestic genes
CN104379180A (zh) 2012-04-10 2015-02-25 佐治亚州立大学研究基金会公司 用cyld抑制剂治疗中耳炎及其它病状的组合物和方法
CN105473722A (zh) 2013-03-11 2016-04-06 先锋国际良种公司 用于改善植物中化学信号扩散的方法及组合物
CA2904132A1 (en) 2013-03-13 2014-10-02 E. I. Du Pont De Nemours And Company Production of small interfering rnas in planta
UA121847C2 (uk) 2013-03-14 2020-08-10 Піонір Хай-Бред Інтернешнл Інк. Полінуклеотид, що кодує елемент сайленсингу з інсектицидною активністю проти шкідника рослин із ряду coleoptera, та спосіб його застосування
EP3842535A1 (en) 2014-04-30 2021-06-30 Fondazione Edmund Mach Plant srna extract or plant mirna for use as immunosuppressive agent
CA2961733A1 (en) 2014-09-17 2016-03-24 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
US10647995B2 (en) 2015-06-16 2020-05-12 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
CA3022858A1 (en) 2016-06-16 2017-12-21 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
WO2018005752A1 (en) * 2016-06-30 2018-01-04 Cold Spring Harbor Laboratory Control of meiotic crossover in maize
US20210292778A1 (en) 2016-07-12 2021-09-23 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
BR112019016394A2 (pt) 2017-02-08 2020-04-07 Pioneer Hi Bred Int construto de dna, pilha molecular, pilha de melhoramento, planta transgênica ou progênie da mesma, composição e método para controlar uma população de praga de inseto
WO2019074598A1 (en) 2017-10-13 2019-04-18 Pioneer Hi-Bred International, Inc. VIRUS-INDUCED GENETIC SILENCING TECHNOLOGY FOR THE CONTROL OF INSECTS IN MAIZE
TW202300650A (zh) 2021-03-26 2023-01-01 美商旗艦先鋒創新有限責任(Vii)公司 真核系統中環狀多核糖核苷酸的產生
WO2022204466A1 (en) 2021-03-26 2022-09-29 Flagship Pioneering Innovations Vii, Llc Production of circular polyribonucleotides in a prokaryotic system
AR125217A1 (es) 2021-03-26 2023-06-28 Flagship Pioneering Innovations Vii Llc Producción de polirribonucleótidos circulares en un sistema procariota

Family Cites Families (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
US5023179A (en) 1988-11-14 1991-06-11 Eric Lam Promoter enhancer element for gene expression in plant roots
US5110732A (en) 1989-03-14 1992-05-05 The Rockefeller University Selective gene expression in plants
KR920701453A (ko) 1989-03-17 1992-08-11 미리엄 디. 멕코나헤이 유전자발현의 외부조절
US5240855A (en) 1989-05-12 1993-08-31 Pioneer Hi-Bred International, Inc. Particle gun
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
ES2187497T3 (es) 1990-04-12 2003-06-16 Syngenta Participations Ag Promotores preferentemente en tejidos.
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
DE69230290T2 (de) 1991-08-27 2000-07-20 Novartis Ag, Basel Proteine mit insektiziden eigenschaften gegen homopteran insekten und ihre verwendung im pflanzenschutz
PT100930B (pt) 1991-10-04 2004-02-27 Univ North Carolina State Plantas transgenicas resistentes aos agentes patogenicos e metodo para a sua producao
US5324646A (en) 1992-01-06 1994-06-28 Pioneer Hi-Bred International, Inc. Methods of regeneration of Medicago sativa and expressing foreign DNA in same
US5428148A (en) 1992-04-24 1995-06-27 Beckman Instruments, Inc. N4 - acylated cytidinyl compounds useful in oligonucleotide synthesis
US5401836A (en) 1992-07-16 1995-03-28 Pioneer Hi-Bre International, Inc. Brassica regulatory sequence for root-specific or root-abundant gene expression
HUT70467A (en) 1992-07-27 1995-10-30 Pioneer Hi Bred Int An improved method of agrobactenium-mediated transformation of cultvred soyhean cells
IL108241A (en) 1992-12-30 2000-08-13 Biosource Genetics Corp Plant expression system comprising a defective tobamovirus replicon integrated into the plant chromosome and a helper virus
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5470353A (en) 1993-10-20 1995-11-28 Hollister Incorporated Post-operative thermal blanket
US5633363A (en) 1994-06-03 1997-05-27 Iowa State University, Research Foundation In Root preferential promoter
US5736369A (en) 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5750868A (en) 1994-12-08 1998-05-12 Pioneer Hi-Bred International, Inc. Reversible nuclear genetic system for male sterility in transgenic plants
US5837876A (en) 1995-07-28 1998-11-17 North Carolina State University Root cortex specific gene promoter
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
WO1998001575A1 (en) 1996-07-08 1998-01-15 Pioneer Hi-Bred International, Inc. Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
EP0990040B1 (en) 1997-07-22 2007-10-24 Pioneer Hi-Bred International, Inc. Genes controlling phytate metabolism in plants and uses thereof
WO1999043819A1 (en) 1998-02-26 1999-09-02 Pioneer Hi-Bred International, Inc. Family of maize pr-1 genes and promoters
EP1056864B1 (en) 1998-02-26 2004-10-06 Pioneer Hi-Bred International, Inc. Constitutive maize promoters
EP1104469B1 (en) 1998-08-20 2005-11-09 Pioneer Hi-Bred International, Inc. Seed-preferred promoters
AU5788299A (en) 1998-08-28 2000-03-21 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from (end) genes
RU2164944C1 (ru) * 1999-12-09 2001-04-10 Институт молекулярной биологии им. В.А. Энгельгардта РАН Способ изменения генетических свойств организма
US6512165B1 (en) 2000-07-10 2003-01-28 Pioneer Hi-Bred International, Inc. Methods for enhancing plant transformation frequencies
MXPA03006190A (es) 2001-01-12 2004-12-03 Pioneer Hi Bred Int Genes de quinasa polifosfato inositol novedosos y usos de los mismos.
CA2461844C (en) 2001-09-27 2011-04-05 Pioneer Hi-Bred International, Inc. Phytate polynucleotides and methods of use
US20040268441A1 (en) 2002-07-19 2004-12-30 University Of South Carolina Compositions and methods for the modulation of gene expression in plants
US20050144669A1 (en) 2003-07-01 2005-06-30 Whitehead Institute For Biomedical Research MicroRNAs in plants
US8106180B2 (en) 2003-08-07 2012-01-31 Whitehead Institute For Biomedical Research Methods and products for expression of micro RNAs
EP1711613B1 (en) * 2003-10-09 2013-04-10 E.I. Du Pont De Nemours And Company Gene silencing by using micro-rna molecules
WO2006044322A2 (en) 2004-10-12 2006-04-27 The Rockefeller University Micrornas
US20060200878A1 (en) * 2004-12-21 2006-09-07 Linda Lutfiyya Recombinant DNA constructs and methods for controlling gene expression
WO2006074400A2 (en) 2005-01-07 2006-07-13 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon State University Method to trigger rna interference
EP1874935B2 (en) 2005-04-04 2023-10-18 E. I. du Pont de Nemours and Company Polynucleotides and methods for making plants resistant to fungal pathogens
US20100163019A1 (en) 2008-10-10 2010-07-01 Michel Chornet Conversion of cellulosic biomass to sugar

Also Published As

Publication number Publication date
EP2573181B1 (en) 2015-03-25
CA2709333C (en) 2016-06-21
US20120329159A1 (en) 2012-12-27
WO2009079548A2 (en) 2009-06-25
EP2573180A3 (en) 2013-06-26
EP2573182B1 (en) 2015-03-25
ZA201004123B (en) 2011-08-31
CA2895049A1 (en) 2009-06-25
US20110033937A1 (en) 2011-02-10
US20120115234A1 (en) 2012-05-10
US8536405B2 (en) 2013-09-17
CA2894910A1 (en) 2009-06-25
RU2013126413A (ru) 2014-12-20
EP2222858A2 (en) 2010-09-01
CA2895048C (en) 2016-10-11
EP2573180B1 (en) 2015-03-25
EP2573181A3 (en) 2013-06-26
US20090155909A1 (en) 2009-06-18
CN101903525A (zh) 2010-12-01
EP2573182A2 (en) 2013-03-27
US20120107939A1 (en) 2012-05-03
CN101903525B (zh) 2014-04-23
US8273951B2 (en) 2012-09-25
CA2894910C (en) 2016-10-11
CA2709333A1 (en) 2009-06-25
WO2009079548A3 (en) 2009-09-17
CA2895044A1 (en) 2009-06-25
EP2573181A2 (en) 2013-03-27
US8115055B2 (en) 2012-02-14
BRPI0819495A2 (pt) 2015-03-10
US8415526B2 (en) 2013-04-09
CN103898114A (zh) 2014-07-02
CA2895049C (en) 2016-10-11
EP2573180A2 (en) 2013-03-27
RU2492239C2 (ru) 2013-09-10
CA2895044C (en) 2016-07-05
CA2895048A1 (en) 2009-06-25
EP2222858B1 (en) 2013-02-13
RU2010129428A (ru) 2012-01-27
CN103898114B (zh) 2016-06-29
EP2573182A3 (en) 2013-06-26

Similar Documents

Publication Publication Date Title
UA101823C2 (ru) Даун-регуляция экспрессии гена с помощью искусственных микрорнк
US9546374B2 (en) Down-regulation of gene expression using artificial microRNAs
US20160017349A1 (en) Maize microrna sequences and targets thereof for agronomic traits
UA113949C2 (xx) Даун-регуляція експресії гена за допомогою штучних мікро-рнк
UA113948C2 (xx) Даун-регуляція експресії гена за допомогою штучних мікро-рнк
UA113950C2 (xx) Даун-регуляція експресії гена за допомогою штучних мікро-рнк