AR051517A1 - Aislamiento y purificacion de proteinas - Google Patents

Aislamiento y purificacion de proteinas

Info

Publication number
AR051517A1
AR051517A1 ARP050104994A ARP050104994A AR051517A1 AR 051517 A1 AR051517 A1 AR 051517A1 AR P050104994 A ARP050104994 A AR P050104994A AR P050104994 A ARP050104994 A AR P050104994A AR 051517 A1 AR051517 A1 AR 051517A1
Authority
AR
Argentina
Prior art keywords
escpr
region
concentration
fusion protein
purifying
Prior art date
Application number
ARP050104994A
Other languages
English (en)
Inventor
Mugica Maria Dolores Ludevid
Virgili Miriam Bastida
Royo Blanca Llompart
Luna Pablo Marzabal
Quetglas Margarita Torrent
Original Assignee
Era Plantech S L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Era Plantech S L filed Critical Era Plantech S L
Publication of AR051517A1 publication Critical patent/AR051517A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/425—Zeins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/585—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Botany (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se describe un procedimiento para purificar una proteína de fusion recombinante que se expresa como ensamblajes similares a cuerpos proteicos recombinantes (ESCPR) en células hospedadoras, en el que se proporciona un homogeneizado acuoso de las células hospedadoras transformada que expresan una proteína de fusion como ESCPR con una densidad predeterminada. En el homogeneizada se forman regiones de diferente densidad, proporcionando una region que contiene una concentracion relativamente enriquecida en ESCPR y una region que contiene una concentracion relativamente baja de ESCPR. La region baja de ESCPR se separa a partir de la region de concentracion relativamente enriquecida en ESCPR, purificando así dicha proteína de fusion. A continuacion, la region de concentracion relativamente enriquecida en ESCPR se recoge segun se desee.
ARP050104994A 2004-11-29 2005-11-29 Aislamiento y purificacion de proteinas AR051517A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB0426161.6A GB0426161D0 (en) 2004-11-29 2004-11-29 Protein isolation and purification

Publications (1)

Publication Number Publication Date
AR051517A1 true AR051517A1 (es) 2007-01-17

Family

ID=33561507

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP050104994A AR051517A1 (es) 2004-11-29 2005-11-29 Aislamiento y purificacion de proteinas

Country Status (12)

Country Link
US (1) US20060123509A1 (es)
EP (1) EP1819820A1 (es)
JP (1) JP2008521767A (es)
KR (1) KR20070091157A (es)
CN (1) CN101103114B (es)
AR (1) AR051517A1 (es)
AU (1) AU2005308922B2 (es)
BR (1) BRPI0517120A (es)
CA (1) CA2589158A1 (es)
GB (1) GB0426161D0 (es)
RU (1) RU2420585C2 (es)
WO (1) WO2006056484A1 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
GB0426160D0 (en) 2004-11-29 2004-12-29 Era Plantech S L Production of proteins
US9555097B2 (en) 2006-02-23 2017-01-31 Era Biotech, S.A. Recombinant protein bodies as immunogen-specific adjuvants
US8163880B2 (en) 2006-02-23 2012-04-24 Era Biotech S.A. Production of biologically active proteins
CN102822191B (zh) * 2010-03-30 2015-06-10 诺维信公司 晶体代谢物回收
EP2418284A1 (en) 2010-08-13 2012-02-15 ERA Biotech, S.A. Protein body-inducing polypeptide sequences
EP2468870A1 (en) * 2010-12-23 2012-06-27 Philip Morris Products S.A. Method for expressing desxoyribonuclease in plants
EP2468871A1 (en) * 2010-12-23 2012-06-27 Philip Morris Products S.A. Method for producing apolipoprotein in plants
CA2824155A1 (en) 2011-01-17 2012-07-26 Philip Morris Products S.A. Vectors for nucleic acid expression in plants
DK2665818T3 (en) 2011-01-17 2017-04-10 Philip Morris Products Sa Protein expression in plants
DE102015107846A1 (de) 2015-05-19 2016-11-24 Johann Wolfgang Goethe-Universität Frankfurt Synthetische Organellen in Hefe
CN105285314A (zh) * 2015-09-14 2016-02-03 哈尔滨工业大学 一种13KDa大米醇溶蛋白的提取方法
CN108503686A (zh) * 2018-05-07 2018-09-07 江苏省中国科学院植物研究所 一种植物总蛋白的提取方法及其专用提取液

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4215040A (en) * 1978-12-22 1980-07-29 The Procter & Gamble Company Density separation process
FR2500937B1 (es) 1981-02-27 1983-04-15 Thomson Csf
US5948682A (en) * 1991-02-22 1999-09-07 Sembiosys Genetics Inc. Preparation of heterologous proteins on oil bodies
ATE346944T1 (de) * 1997-09-30 2006-12-15 Univ California Herstellung von proteinen in pflanzensamen
DK1532261T3 (da) * 2002-05-24 2010-05-31 Medtronic Inc Fremgangsmåder og DNA-konstrukter til produktion af polypeptider med højt udbytte
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
GB0426160D0 (en) * 2004-11-29 2004-12-29 Era Plantech S L Production of proteins
US8163880B2 (en) * 2006-02-23 2012-04-24 Era Biotech S.A. Production of biologically active proteins
US9555097B2 (en) * 2006-02-23 2017-01-31 Era Biotech, S.A. Recombinant protein bodies as immunogen-specific adjuvants
EP2418284A1 (en) * 2010-08-13 2012-02-15 ERA Biotech, S.A. Protein body-inducing polypeptide sequences

Also Published As

Publication number Publication date
WO2006056484A1 (en) 2006-06-01
JP2008521767A (ja) 2008-06-26
BRPI0517120A (pt) 2008-09-30
CN101103114B (zh) 2013-01-02
RU2420585C2 (ru) 2011-06-10
CN101103114A (zh) 2008-01-09
CA2589158A1 (en) 2006-06-01
RU2007124377A (ru) 2009-01-10
US20060123509A1 (en) 2006-06-08
AU2005308922B2 (en) 2011-07-28
GB0426161D0 (en) 2004-12-29
AU2005308922A1 (en) 2006-06-01
EP1819820A1 (en) 2007-08-22
KR20070091157A (ko) 2007-09-07

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