AR041644A1 - Metodo para la produccion de proteinas recombinantes en la glandula mamaria de mamiferos no transgenicos - Google Patents

Metodo para la produccion de proteinas recombinantes en la glandula mamaria de mamiferos no transgenicos

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Publication number
AR041644A1
AR041644A1 ARP030103775A ARP030103775A AR041644A1 AR 041644 A1 AR041644 A1 AR 041644A1 AR P030103775 A ARP030103775 A AR P030103775A AR P030103775 A ARP030103775 A AR P030103775A AR 041644 A1 AR041644 A1 AR 041644A1
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AR
Argentina
Prior art keywords
mammary gland
proteins
milk
interest
production
Prior art date
Application number
ARP030103775A
Other languages
English (en)
Inventor
Alonso Jorge Roberto Toledo
Ramos Oliberto Sanchez
Molto Maria Del Pilar Rodriguez
Reboredo Fidel Ovidio Castro
Original Assignee
Ct Ingenieria Genetica Biotech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Ct Ingenieria Genetica Biotech filed Critical Ct Ingenieria Genetica Biotech
Publication of AR041644A1 publication Critical patent/AR041644A1/es

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    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475Growth factors; Growth regulators
    • C07K14/505Erythropoietin [EPO]
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
    • C12N15/861Adenoviral vectors
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/61Growth hormone [GH], i.e. somatotropin
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00Modified animals
    • A01K2207/15Humanized animals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00Animals characterised by species
    • A01K2227/10Mammal
    • A01K2227/102Caprine
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00Animals characterised by species
    • A01K2227/10Mammal
    • A01K2227/105Murine
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00Animals characterised by purpose
    • A01K2267/01Animal expressing industrially exogenous proteins
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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    • C12N2710/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011Details
    • C12N2710/10011Adenoviridae
    • C12N2710/10311Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12N2800/00Nucleic acids vectors
    • C12N2800/30Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
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    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/008Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2840/00Vectors comprising a special translation-regulating system
    • C12N2840/20Vectors comprising a special translation-regulating system translation of more than one cistron

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Método que permite la producción de proteínas de interés bio-farmacéutico a partir de la glándula mamaria de animales no transgénicos. Los genes que codifican para dichas proteínas son transferidos a las células epiteliales mamarias empleando vectores adenovirales. Tales vectores pueden estar basados en adenovirus con replicación defectiva como el DE1DE3, o en adenovirus dependientes de ayudante a los cuales se les han eliminado todas o casi todas las secuencias adenovirus codificantes. Mediante este método es posible la producción altos niveles de proteínas recombinantes en la leche permitiendo de esta forma la producción a gran escala de proteínas de interés biofarmacéutico en su forma biológicamente activa. Reivindicación 1: Un método para la producción de proteínas heterólogas en la leche de mamíferos no humanos mediante la transformación del EGM con vectores adenovirales, que comprende los siguientes pasos: a) Inducción de la lactación de una mamífero en etapas tempranas de su madurez sexual; b) Remoción de la leche y lavado exhaustivo del interior de la glándula mamaria previo a la infusión viral; c) Infusión a través del canal del pezón hasta completar la capacidad de la glándula mamaria de una solución conteniendo vectores adenovirales que portan los genes que codifican para las proteínas heterólogas de interés; d) Vaciado de la glándula mamaria entre las 4 y 24 horas post infusión; e) Colecta de la leche contenida en la glándula mamaria a partir de las 48 horas post infusión; f) Purificación a partir de la leche de las proteínas heterólogas de interés.
ARP030103775A 2002-10-21 2003-10-16 Metodo para la produccion de proteinas recombinantes en la glandula mamaria de mamiferos no transgenicos AR041644A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU20020235A CU23102A1 (es) 2002-10-21 2002-10-21 Método para la producción de proteínas recombinantes en la glándula mamaria de mamíferos no transgénicos

Publications (1)

Publication Number Publication Date
AR041644A1 true AR041644A1 (es) 2005-05-26

Family

ID=40134844

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP030103775A AR041644A1 (es) 2002-10-21 2003-10-16 Metodo para la produccion de proteinas recombinantes en la glandula mamaria de mamiferos no transgenicos

Country Status (13)

Country Link
EP (1) EP1557084A2 (es)
JP (1) JP2006503560A (es)
KR (1) KR20050083791A (es)
CN (1) CN1744814A (es)
AR (1) AR041644A1 (es)
AU (1) AU2003273726B8 (es)
BR (1) BR0315555A (es)
CA (1) CA2502902A1 (es)
CU (1) CU23102A1 (es)
MX (1) MXPA05004295A (es)
RU (1) RU2345088C2 (es)
WO (1) WO2004034780A2 (es)
ZA (1) ZA200503166B (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CU23544A1 (es) * 2006-02-28 2010-06-17 Ct Ingenieria Genetica Biotech Antígenos vacunales quiméricos contra el virus de la peste porcina clásica
CU23576A1 (es) * 2006-02-28 2010-09-30 Ct Ingenieria Genetica Biotech Antígenos vacunales quiméricos contra el virus de la influenza aviar
CN1869217B (zh) * 2006-05-22 2013-08-07 李青旺 以腺病毒为载体乳腺表达生产转基因蛋白药物的方法
WO2010002160A2 (ko) 2008-06-30 2010-01-07 조아제약주식회사 돼지의 알파에스 1 카제인 유전자, 그 프로모터 및 그의 용도
WO2013096458A1 (en) * 2011-12-19 2013-06-27 Gtc Biotherapeutics, Inc. Recombinant human alpha-1-antitrypsin for the treatment of inflammatory disorders
CL2014002410A1 (es) * 2014-09-12 2014-11-14 Univ Concepcion Método para la producción de proteínas recombinantes en glándula mamaria de mamíferos mediante la transformación del epítelio glandular mamario con vectores adenoasociados.
WO2018085967A1 (en) * 2016-11-08 2018-05-17 Guangzhou Institutes Of Biomedicine And Health, Chinese Academy Of Sciences Genetically humanized mammals expressing human serum albumin and uses thereof
EP4003957B1 (en) 2019-07-25 2024-04-17 Medichem, S.A. Process for the synthesis of n-alkyl-4-pyridinamines

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5780009A (en) * 1995-01-20 1998-07-14 Nexia Biotechnologies, Inc. Direct gene transfer into the ruminant mammary gland
AU7719596A (en) * 1995-11-07 1997-05-29 Baylor College Of Medicine Adenovirus-mediated production of bioactive proteins by mammalian cells and animals
EP0954579A1 (en) * 1996-06-20 1999-11-10 Merck & Co., Inc. Gene therapy for obesity
NZ506478A (en) * 1998-02-24 2003-07-25 Ca Minister Agriculture & Food Trans-somatics with gene transfer into mammary epithelial cells
AU2002228730A1 (en) * 2000-10-27 2002-05-06 Beth Israel Deaconess Medical Center, Inc. Mammary secreted protein 36 (msp36) for cancer treatment and diagnosis

Also Published As

Publication number Publication date
AU2003273726A1 (en) 2004-05-04
RU2005115495A (ru) 2005-11-20
CA2502902A1 (en) 2004-04-29
CU23102A1 (es) 2005-12-20
KR20050083791A (ko) 2005-08-26
AU2003273726B2 (en) 2009-07-09
BR0315555A (pt) 2005-08-23
WO2004034780A2 (es) 2004-04-29
EP1557084A2 (en) 2005-07-27
ZA200503166B (en) 2006-10-25
RU2345088C2 (ru) 2009-01-27
JP2006503560A (ja) 2006-02-02
WO2004034780A3 (es) 2004-05-27
MXPA05004295A (es) 2005-08-03
CN1744814A (zh) 2006-03-08
AU2003273726B8 (en) 2009-09-03

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