AR041644A1 - Metodo para la produccion de proteinas recombinantes en la glandula mamaria de mamiferos no transgenicos - Google Patents

Metodo para la produccion de proteinas recombinantes en la glandula mamaria de mamiferos no transgenicos

Info

Publication number
AR041644A1
AR041644A1 ARP030103775A ARP030103775A AR041644A1 AR 041644 A1 AR041644 A1 AR 041644A1 AR P030103775 A ARP030103775 A AR P030103775A AR P030103775 A ARP030103775 A AR P030103775A AR 041644 A1 AR041644 A1 AR 041644A1
Authority
AR
Argentina
Prior art keywords
mammary gland
proteins
milk
interest
production
Prior art date
Application number
ARP030103775A
Other languages
English (en)
Inventor
Alonso Jorge Roberto Toledo
Ramos Oliberto Sanchez
Molto Maria Del Pilar Rodriguez
Reboredo Fidel Ovidio Castro
Original Assignee
Ct Ingenieria Genetica Biotech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ct Ingenieria Genetica Biotech filed Critical Ct Ingenieria Genetica Biotech
Publication of AR041644A1 publication Critical patent/AR041644A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/861—Adenoviral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/102—Caprine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Método que permite la producción de proteínas de interés bio-farmacéutico a partir de la glándula mamaria de animales no transgénicos. Los genes que codifican para dichas proteínas son transferidos a las células epiteliales mamarias empleando vectores adenovirales. Tales vectores pueden estar basados en adenovirus con replicación defectiva como el DE1DE3, o en adenovirus dependientes de ayudante a los cuales se les han eliminado todas o casi todas las secuencias adenovirus codificantes. Mediante este método es posible la producción altos niveles de proteínas recombinantes en la leche permitiendo de esta forma la producción a gran escala de proteínas de interés biofarmacéutico en su forma biológicamente activa. Reivindicación 1: Un método para la producción de proteínas heterólogas en la leche de mamíferos no humanos mediante la transformación del EGM con vectores adenovirales, que comprende los siguientes pasos: a) Inducción de la lactación de una mamífero en etapas tempranas de su madurez sexual; b) Remoción de la leche y lavado exhaustivo del interior de la glándula mamaria previo a la infusión viral; c) Infusión a través del canal del pezón hasta completar la capacidad de la glándula mamaria de una solución conteniendo vectores adenovirales que portan los genes que codifican para las proteínas heterólogas de interés; d) Vaciado de la glándula mamaria entre las 4 y 24 horas post infusión; e) Colecta de la leche contenida en la glándula mamaria a partir de las 48 horas post infusión; f) Purificación a partir de la leche de las proteínas heterólogas de interés.
ARP030103775A 2002-10-21 2003-10-16 Metodo para la produccion de proteinas recombinantes en la glandula mamaria de mamiferos no transgenicos AR041644A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU20020235A CU23102A1 (es) 2002-10-21 2002-10-21 Método para la producción de proteínas recombinantes en la glándula mamaria de mamíferos no transgénicos

Publications (1)

Publication Number Publication Date
AR041644A1 true AR041644A1 (es) 2005-05-26

Family

ID=40134844

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP030103775A AR041644A1 (es) 2002-10-21 2003-10-16 Metodo para la produccion de proteinas recombinantes en la glandula mamaria de mamiferos no transgenicos

Country Status (13)

Country Link
EP (1) EP1557084A2 (es)
JP (1) JP2006503560A (es)
KR (1) KR20050083791A (es)
CN (1) CN1744814A (es)
AR (1) AR041644A1 (es)
AU (1) AU2003273726B8 (es)
BR (1) BR0315555A (es)
CA (1) CA2502902A1 (es)
CU (1) CU23102A1 (es)
MX (1) MXPA05004295A (es)
RU (1) RU2345088C2 (es)
WO (1) WO2004034780A2 (es)
ZA (1) ZA200503166B (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CU23576A1 (es) * 2006-02-28 2010-09-30 Ct Ingenieria Genetica Biotech Antígenos vacunales quiméricos contra el virus de la influenza aviar
CU23544A1 (es) * 2006-02-28 2010-06-17 Ct Ingenieria Genetica Biotech Antígenos vacunales quiméricos contra el virus de la peste porcina clásica
CN1869217B (zh) * 2006-05-22 2013-08-07 李青旺 以腺病毒为载体乳腺表达生产转基因蛋白药物的方法
US9738694B2 (en) 2008-06-30 2017-08-22 Cho-A Pharm. Co., Ltd. Gene of porcine alpha-s1 casein, a promoter of the same and use thereof
KR20140132706A (ko) * 2011-12-19 2014-11-18 엘에프비 유에스에이, 인크. 염증성 장애의 치료를 위한 재조합 인간 알파-1-안티트립신
CL2014002410A1 (es) * 2014-09-12 2014-11-14 Univ Concepcion Método para la producción de proteínas recombinantes en glándula mamaria de mamíferos mediante la transformación del epítelio glandular mamario con vectores adenoasociados.
WO2018085967A1 (en) * 2016-11-08 2018-05-17 Guangzhou Institutes Of Biomedicine And Health, Chinese Academy Of Sciences Genetically humanized mammals expressing human serum albumin and uses thereof
ES2980492T3 (es) 2019-07-25 2024-10-01 Medichem S A Procedimiento para la síntesis de N-alquil-4-piridinaminas

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5780009A (en) * 1995-01-20 1998-07-14 Nexia Biotechnologies, Inc. Direct gene transfer into the ruminant mammary gland
AU7719596A (en) * 1995-11-07 1997-05-29 Baylor College Of Medicine Adenovirus-mediated production of bioactive proteins by mammalian cells and animals
EP0954579A1 (en) * 1996-06-20 1999-11-10 Merck & Co., Inc. Gene therapy for obesity
WO1999043795A1 (en) * 1998-02-24 1999-09-02 Her Majesty In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada Trans-somatics with gene transfer into mammary epithelial cells
WO2002034282A2 (en) * 2000-10-27 2002-05-02 Beth Israel Deaconess Medical Center, Inc. Mammary secreted protein 36 (msp36) for cancer treatment and diagnosis

Also Published As

Publication number Publication date
JP2006503560A (ja) 2006-02-02
KR20050083791A (ko) 2005-08-26
AU2003273726B2 (en) 2009-07-09
RU2005115495A (ru) 2005-11-20
AU2003273726B8 (en) 2009-09-03
WO2004034780A3 (es) 2004-05-27
ZA200503166B (en) 2006-10-25
RU2345088C2 (ru) 2009-01-27
CN1744814A (zh) 2006-03-08
CU23102A1 (es) 2005-12-20
WO2004034780A2 (es) 2004-04-29
MXPA05004295A (es) 2005-08-03
AU2003273726A1 (en) 2004-05-04
CA2502902A1 (en) 2004-04-29
BR0315555A (pt) 2005-08-23
EP1557084A2 (en) 2005-07-27

Similar Documents

Publication Publication Date Title
CN105985985B (zh) Crispr技术编辑并用igf优化的异体间充质干细胞的制备方法及在治疗心梗中应用
Tiburcy et al. Defined engineered human myocardium with advanced maturation for applications in heart failure modeling and repair
Costantini et al. Engineering muscle networks in 3D gelatin methacryloyl hydrogels: influence of mechanical stiffness and geometrical confinement
CN102573943B (zh) 通过活化富血小板血浆(prp)诱导组织再生的组合物及其制备方法
AR041644A1 (es) Metodo para la produccion de proteinas recombinantes en la glandula mamaria de mamiferos no transgenicos
ES2615102T3 (es) Vaso sanguíneo alogénico obtenido mediante bioingeniería
US20120177611A1 (en) Elastic substrates and methods of use in cell manipulation and culture
ES2927461T3 (es) Células madre mesenquimatosas derivadas de tejido perinatal: método de preparación y utilizaciones de las mismas
ES2307801T3 (es) Precursor de la seda para formar filamentos.
EP2566951B1 (en) Method of reseeding adherent cells grown in a hollow fiber bioreactor system
GB2438352A (en) Cell culture chamber
WO2008053892A1 (fr) Produit pharmaceutique servant à favoriser la régénération fonctionnelle d'un tissu endommagé
AR018038A1 (es) Un procedimiento para producir un embrion animal, y los animales que se obtienen empleando este procedimiento
CN1986006A (zh) 生物型神经导管
CN106730034A (zh) 基于切片式去细胞支架构建的人工神经移植物及制备方法
CN110747159A (zh) 一种小鼠或大鼠肾成纤维细胞分离及传代培养方法
JP2008531485A5 (es)
Laitinen et al. The isolation and culture of human cord blood-derived mesenchymal stem cells under low oxygen conditions
CN113599531B (zh) Pcm多肽组合kala多肽的红细胞仿生纳米材料的应用及其制备方法
JP6382938B2 (ja) 細胞培養治具およびこの細胞培養治具を用いた細胞培養方法
Kim et al. Patterning of interconnected human brain spheroids
CN110669792A (zh) 基因修饰间充质干细胞、制备方法、应用及细胞治疗产品
ES2681424T3 (es) Protransducina B, un potenciador de la transferencia génica
AR019864A1 (es) Proceso para producir el antigeno de toxoplasma sag1 o fragmentos del mismo; proteina sag1 o fragmentos de la misma expresada en dicho proceso;composiciones de vacuna que comprendan dicha proteina sag1 o fragmentos de la misma; uso de dicha proteina para la manufactura de medicamentos para preveniry
CN105497983A (zh) 一种简单高效的干细胞贴片制备方法

Legal Events

Date Code Title Description
FC Refusal