AR041644A1 - Metodo para la produccion de proteinas recombinantes en la glandula mamaria de mamiferos no transgenicos - Google Patents
Metodo para la produccion de proteinas recombinantes en la glandula mamaria de mamiferos no transgenicosInfo
- Publication number
- AR041644A1 AR041644A1 ARP030103775A ARP030103775A AR041644A1 AR 041644 A1 AR041644 A1 AR 041644A1 AR P030103775 A ARP030103775 A AR P030103775A AR P030103775 A ARP030103775 A AR P030103775A AR 041644 A1 AR041644 A1 AR 041644A1
- Authority
- AR
- Argentina
- Prior art keywords
- mammary gland
- proteins
- milk
- interest
- production
- Prior art date
Links
- 210000005075 mammary gland Anatomy 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 241000124008 Mammalia Species 0.000 title abstract 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 8
- 102000004169 proteins and genes Human genes 0.000 abstract 6
- 239000008267 milk Substances 0.000 abstract 5
- 210000004080 milk Anatomy 0.000 abstract 5
- 235000013336 milk Nutrition 0.000 abstract 5
- 238000001802 infusion Methods 0.000 abstract 4
- 239000013598 vector Substances 0.000 abstract 4
- 241000701161 unidentified adenovirus Species 0.000 abstract 3
- 229960000074 biopharmaceutical Drugs 0.000 abstract 2
- 241001465754 Metazoa Species 0.000 abstract 1
- 230000002950 deficient Effects 0.000 abstract 1
- 230000001419 dependent effect Effects 0.000 abstract 1
- 210000002919 epithelial cell Anatomy 0.000 abstract 1
- 230000006698 induction Effects 0.000 abstract 1
- 230000006651 lactation Effects 0.000 abstract 1
- 238000011031 large-scale manufacturing process Methods 0.000 abstract 1
- 210000002445 nipple Anatomy 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 230000010076 replication Effects 0.000 abstract 1
- 230000001568 sexual effect Effects 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
- 230000003612 virological effect Effects 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/505—Erythropoietin [EPO]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
- C12N15/861—Adenoviral vectors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2207/00—Modified animals
- A01K2207/15—Humanized animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/102—Caprine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/01—Animal expressing industrially exogenous proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10341—Use of virus, viral particle or viral elements as a vector
- C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Endocrinology (AREA)
- Virology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Método que permite la producción de proteínas de interés bio-farmacéutico a partir de la glándula mamaria de animales no transgénicos. Los genes que codifican para dichas proteínas son transferidos a las células epiteliales mamarias empleando vectores adenovirales. Tales vectores pueden estar basados en adenovirus con replicación defectiva como el DE1DE3, o en adenovirus dependientes de ayudante a los cuales se les han eliminado todas o casi todas las secuencias adenovirus codificantes. Mediante este método es posible la producción altos niveles de proteínas recombinantes en la leche permitiendo de esta forma la producción a gran escala de proteínas de interés biofarmacéutico en su forma biológicamente activa. Reivindicación 1: Un método para la producción de proteínas heterólogas en la leche de mamíferos no humanos mediante la transformación del EGM con vectores adenovirales, que comprende los siguientes pasos: a) Inducción de la lactación de una mamífero en etapas tempranas de su madurez sexual; b) Remoción de la leche y lavado exhaustivo del interior de la glándula mamaria previo a la infusión viral; c) Infusión a través del canal del pezón hasta completar la capacidad de la glándula mamaria de una solución conteniendo vectores adenovirales que portan los genes que codifican para las proteínas heterólogas de interés; d) Vaciado de la glándula mamaria entre las 4 y 24 horas post infusión; e) Colecta de la leche contenida en la glándula mamaria a partir de las 48 horas post infusión; f) Purificación a partir de la leche de las proteínas heterólogas de interés.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CU20020235A CU23102A1 (es) | 2002-10-21 | 2002-10-21 | Método para la producción de proteínas recombinantes en la glándula mamaria de mamíferos no transgénicos |
Publications (1)
Publication Number | Publication Date |
---|---|
AR041644A1 true AR041644A1 (es) | 2005-05-26 |
Family
ID=40134844
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP030103775A AR041644A1 (es) | 2002-10-21 | 2003-10-16 | Metodo para la produccion de proteinas recombinantes en la glandula mamaria de mamiferos no transgenicos |
Country Status (13)
Country | Link |
---|---|
EP (1) | EP1557084A2 (es) |
JP (1) | JP2006503560A (es) |
KR (1) | KR20050083791A (es) |
CN (1) | CN1744814A (es) |
AR (1) | AR041644A1 (es) |
AU (1) | AU2003273726B8 (es) |
BR (1) | BR0315555A (es) |
CA (1) | CA2502902A1 (es) |
CU (1) | CU23102A1 (es) |
MX (1) | MXPA05004295A (es) |
RU (1) | RU2345088C2 (es) |
WO (1) | WO2004034780A2 (es) |
ZA (1) | ZA200503166B (es) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CU23544A1 (es) * | 2006-02-28 | 2010-06-17 | Ct Ingenieria Genetica Biotech | Antígenos vacunales quiméricos contra el virus de la peste porcina clásica |
CU23576A1 (es) * | 2006-02-28 | 2010-09-30 | Ct Ingenieria Genetica Biotech | Antígenos vacunales quiméricos contra el virus de la influenza aviar |
CN1869217B (zh) * | 2006-05-22 | 2013-08-07 | 李青旺 | 以腺病毒为载体乳腺表达生产转基因蛋白药物的方法 |
WO2010002160A2 (ko) | 2008-06-30 | 2010-01-07 | 조아제약주식회사 | 돼지의 알파에스 1 카제인 유전자, 그 프로모터 및 그의 용도 |
WO2013096458A1 (en) * | 2011-12-19 | 2013-06-27 | Gtc Biotherapeutics, Inc. | Recombinant human alpha-1-antitrypsin for the treatment of inflammatory disorders |
CL2014002410A1 (es) * | 2014-09-12 | 2014-11-14 | Univ Concepcion | Método para la producción de proteínas recombinantes en glándula mamaria de mamíferos mediante la transformación del epítelio glandular mamario con vectores adenoasociados. |
WO2018085967A1 (en) * | 2016-11-08 | 2018-05-17 | Guangzhou Institutes Of Biomedicine And Health, Chinese Academy Of Sciences | Genetically humanized mammals expressing human serum albumin and uses thereof |
EP4003957B1 (en) | 2019-07-25 | 2024-04-17 | Medichem, S.A. | Process for the synthesis of n-alkyl-4-pyridinamines |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5780009A (en) * | 1995-01-20 | 1998-07-14 | Nexia Biotechnologies, Inc. | Direct gene transfer into the ruminant mammary gland |
AU7719596A (en) * | 1995-11-07 | 1997-05-29 | Baylor College Of Medicine | Adenovirus-mediated production of bioactive proteins by mammalian cells and animals |
EP0954579A1 (en) * | 1996-06-20 | 1999-11-10 | Merck & Co., Inc. | Gene therapy for obesity |
NZ506478A (en) * | 1998-02-24 | 2003-07-25 | Ca Minister Agriculture & Food | Trans-somatics with gene transfer into mammary epithelial cells |
AU2002228730A1 (en) * | 2000-10-27 | 2002-05-06 | Beth Israel Deaconess Medical Center, Inc. | Mammary secreted protein 36 (msp36) for cancer treatment and diagnosis |
-
2002
- 2002-10-21 CU CU20020235A patent/CU23102A1/es unknown
-
2003
- 2003-10-16 AR ARP030103775A patent/AR041644A1/es not_active Application Discontinuation
- 2003-10-20 CA CA002502902A patent/CA2502902A1/en not_active Abandoned
- 2003-10-20 RU RU2005115495/13A patent/RU2345088C2/ru active
- 2003-10-20 WO PCT/CU2003/000011 patent/WO2004034780A2/es active Application Filing
- 2003-10-20 KR KR1020057006830A patent/KR20050083791A/ko not_active Application Discontinuation
- 2003-10-20 EP EP03757653A patent/EP1557084A2/en not_active Withdrawn
- 2003-10-20 AU AU2003273726A patent/AU2003273726B8/en not_active Expired - Fee Related
- 2003-10-20 BR BR0315555-2A patent/BR0315555A/pt not_active IP Right Cessation
- 2003-10-20 JP JP2004543918A patent/JP2006503560A/ja active Pending
- 2003-10-20 MX MXPA05004295A patent/MXPA05004295A/es unknown
- 2003-10-20 CN CNA2003801034448A patent/CN1744814A/zh active Pending
-
2005
- 2005-04-19 ZA ZA200503166A patent/ZA200503166B/en unknown
Also Published As
Publication number | Publication date |
---|---|
AU2003273726A1 (en) | 2004-05-04 |
RU2005115495A (ru) | 2005-11-20 |
CA2502902A1 (en) | 2004-04-29 |
CU23102A1 (es) | 2005-12-20 |
KR20050083791A (ko) | 2005-08-26 |
AU2003273726B2 (en) | 2009-07-09 |
BR0315555A (pt) | 2005-08-23 |
WO2004034780A2 (es) | 2004-04-29 |
EP1557084A2 (en) | 2005-07-27 |
ZA200503166B (en) | 2006-10-25 |
RU2345088C2 (ru) | 2009-01-27 |
JP2006503560A (ja) | 2006-02-02 |
WO2004034780A3 (es) | 2004-05-27 |
MXPA05004295A (es) | 2005-08-03 |
CN1744814A (zh) | 2006-03-08 |
AU2003273726B8 (en) | 2009-09-03 |
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