RU2005115495A - Способ получения рекомбинантных белков в молочной железе не трансгенных млекопитающих - Google Patents
Способ получения рекомбинантных белков в молочной железе не трансгенных млекопитающих Download PDFInfo
- Publication number
- RU2005115495A RU2005115495A RU2005115495/13A RU2005115495A RU2005115495A RU 2005115495 A RU2005115495 A RU 2005115495A RU 2005115495/13 A RU2005115495/13 A RU 2005115495/13A RU 2005115495 A RU2005115495 A RU 2005115495A RU 2005115495 A RU2005115495 A RU 2005115495A
- Authority
- RU
- Russia
- Prior art keywords
- proteins
- milk
- infusion
- breast
- interest
- Prior art date
Links
- 241000124008 Mammalia Species 0.000 title claims 3
- 210000000481 breast Anatomy 0.000 title claims 3
- 238000004519 manufacturing process Methods 0.000 title claims 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 claims 8
- 102000004169 proteins and genes Human genes 0.000 claims 5
- 238000001802 infusion Methods 0.000 claims 4
- 239000008267 milk Substances 0.000 claims 4
- 210000004080 milk Anatomy 0.000 claims 4
- 235000013336 milk Nutrition 0.000 claims 4
- 239000013598 vector Substances 0.000 claims 4
- 238000000034 method Methods 0.000 claims 3
- 241000701161 unidentified adenovirus Species 0.000 claims 3
- 210000005075 mammary gland Anatomy 0.000 claims 2
- 108010039209 Blood Coagulation Factors Proteins 0.000 claims 1
- 102000015081 Blood Coagulation Factors Human genes 0.000 claims 1
- 102100025064 Cellular tumor antigen p53 Human genes 0.000 claims 1
- 102100026735 Coagulation factor VIII Human genes 0.000 claims 1
- 102000004127 Cytokines Human genes 0.000 claims 1
- 108090000695 Cytokines Proteins 0.000 claims 1
- 101800003838 Epidermal growth factor Proteins 0.000 claims 1
- 102400001368 Epidermal growth factor Human genes 0.000 claims 1
- 108090000394 Erythropoietin Proteins 0.000 claims 1
- 102000003951 Erythropoietin Human genes 0.000 claims 1
- 108091005904 Hemoglobin subunit beta Proteins 0.000 claims 1
- 241000282412 Homo Species 0.000 claims 1
- 101000721661 Homo sapiens Cellular tumor antigen p53 Proteins 0.000 claims 1
- 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 claims 1
- 102000002265 Human Growth Hormone Human genes 0.000 claims 1
- 108010000521 Human Growth Hormone Proteins 0.000 claims 1
- 239000000854 Human Growth Hormone Substances 0.000 claims 1
- 102000008100 Human Serum Albumin Human genes 0.000 claims 1
- 108091006905 Human Serum Albumin Proteins 0.000 claims 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 claims 1
- 102000014150 Interferons Human genes 0.000 claims 1
- 108010050904 Interferons Proteins 0.000 claims 1
- 102000000588 Interleukin-2 Human genes 0.000 claims 1
- 108010002350 Interleukin-2 Proteins 0.000 claims 1
- 102000004889 Interleukin-6 Human genes 0.000 claims 1
- 108090001005 Interleukin-6 Proteins 0.000 claims 1
- 108010025020 Nerve Growth Factor Proteins 0.000 claims 1
- 102000015336 Nerve Growth Factor Human genes 0.000 claims 1
- 101800004937 Protein C Proteins 0.000 claims 1
- 102000017975 Protein C Human genes 0.000 claims 1
- 101800001700 Saposin-D Proteins 0.000 claims 1
- 102000013275 Somatomedins Human genes 0.000 claims 1
- 102000003978 Tissue Plasminogen Activator Human genes 0.000 claims 1
- 108090000373 Tissue Plasminogen Activator Proteins 0.000 claims 1
- 108010040002 Tumor Suppressor Proteins Proteins 0.000 claims 1
- 102000001742 Tumor Suppressor Proteins Human genes 0.000 claims 1
- 241000700605 Viruses Species 0.000 claims 1
- 102000015395 alpha 1-Antitrypsin Human genes 0.000 claims 1
- 108010050122 alpha 1-Antitrypsin Proteins 0.000 claims 1
- 229940024142 alpha 1-antitrypsin Drugs 0.000 claims 1
- -1 antibodies Proteins 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 239000003114 blood coagulation factor Substances 0.000 claims 1
- 229940116977 epidermal growth factor Drugs 0.000 claims 1
- 210000002919 epithelial cell Anatomy 0.000 claims 1
- 229940105423 erythropoietin Drugs 0.000 claims 1
- 239000003102 growth factor Substances 0.000 claims 1
- 230000006698 induction Effects 0.000 claims 1
- 229940047124 interferons Drugs 0.000 claims 1
- 229940100601 interleukin-6 Drugs 0.000 claims 1
- 230000006651 lactation Effects 0.000 claims 1
- 230000001404 mediated effect Effects 0.000 claims 1
- 229940053128 nerve growth factor Drugs 0.000 claims 1
- 210000002445 nipple Anatomy 0.000 claims 1
- 239000002245 particle Substances 0.000 claims 1
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 claims 1
- 229960000856 protein c Drugs 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 230000004936 stimulating effect Effects 0.000 claims 1
- 229960000187 tissue plasminogen activator Drugs 0.000 claims 1
- 230000009466 transformation Effects 0.000 claims 1
- 239000000225 tumor suppressor protein Substances 0.000 claims 1
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 claims 1
- 230000003612 virological effect Effects 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/505—Erythropoietin [EPO]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
- C12N15/861—Adenoviral vectors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2207/00—Modified animals
- A01K2207/15—Humanized animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/102—Caprine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/01—Animal expressing industrially exogenous proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10341—Use of virus, viral particle or viral elements as a vector
- C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Virology (AREA)
- Endocrinology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (4)
1. Способ получения гетерологичных белков в молоке млекопитающих, кроме человека, опосредованный трансформацией эпителиальных клеток молочной железы (MGE) аденовирусными векторами, который включает следующие этапы:
а) индукцию лактации млекопитающего на ранних стадиях его половой зрелости;
b) удаление молока и тщательную промывку молочной железы перед инфузией вируса;
с) инфузию через канал соска до полного заполнения всей емкости молочной железы раствором, содержащим аденовирусные векторы, несущие гены, кодирующие интересующие гетерологичные белки;
d) опорожнение молочной железы в течение 4-24 ч после инфузии.
e) сбор молока, содержащегося в молочной железе, начиная через 48 ч после инфузии;
f) очистка из молока интересующих гетерологичных белков.
2. Способ по п.1, в котором аденовирусный вектор содержит большинство аденовирусных генов.
3. Способ по п.1, в котором аденовирусный вектор представляет собой аденовирус, лишенный большинства или всех аденовирусных генов, и для образования вирусных частиц которого требуется аденовирус-помощник, который обеспечивает транскрипцию всех необходимых белков.
4. Способ по п.1, в котором интересующие белки выбраны из группы, включающей факторы роста, такие как гормон роста человека, эпидермальный фактор роста, инсулиноподобный фактор роста, гранулоцитарно-макрофагальный стимулирующий фактор, фактор роста нервов, эритропоэтин, факторы свертываемости, такие как FVIII и FIX, антитела, цитокины, такие как интерлейкин-6 или интерлейкин-2, α1-антитрипсин, сывороточный альбумин человека, β-глобины, тканевой активатор плазминогена, супрессирующие опухоль белки, такие как р53, белок С или интерфероны.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CUCU2002/0235 | 2002-10-21 | ||
| CU20020235A CU23102A1 (es) | 2002-10-21 | 2002-10-21 | Método para la producción de proteínas recombinantes en la glándula mamaria de mamíferos no transgénicos |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2005115495A true RU2005115495A (ru) | 2005-11-20 |
| RU2345088C2 RU2345088C2 (ru) | 2009-01-27 |
Family
ID=40134844
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2005115495/13A RU2345088C2 (ru) | 2002-10-21 | 2003-10-20 | Способ получения рекомбинантных белков в молочной железе нетрансгенных млекопитающих |
Country Status (13)
| Country | Link |
|---|---|
| EP (1) | EP1557084A2 (ru) |
| JP (1) | JP2006503560A (ru) |
| KR (1) | KR20050083791A (ru) |
| CN (1) | CN1744814A (ru) |
| AR (1) | AR041644A1 (ru) |
| AU (1) | AU2003273726B8 (ru) |
| BR (1) | BR0315555A (ru) |
| CA (1) | CA2502902A1 (ru) |
| CU (1) | CU23102A1 (ru) |
| MX (1) | MXPA05004295A (ru) |
| RU (1) | RU2345088C2 (ru) |
| WO (1) | WO2004034780A2 (ru) |
| ZA (1) | ZA200503166B (ru) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CU23576A1 (es) * | 2006-02-28 | 2010-09-30 | Ct Ingenieria Genetica Biotech | Antígenos vacunales quiméricos contra el virus de la influenza aviar |
| CU23544A1 (es) | 2006-02-28 | 2010-06-17 | Ct Ingenieria Genetica Biotech | Antígenos vacunales quiméricos contra el virus de la peste porcina clásica |
| CN1869217B (zh) * | 2006-05-22 | 2013-08-07 | 李青旺 | 以腺病毒为载体乳腺表达生产转基因蛋白药物的方法 |
| JP5507555B2 (ja) * | 2008-06-30 | 2014-05-28 | チョ−エー・ファーム・カンパニー・リミテッド | 豚のαＳ１カゼイン遺伝子、そのプロモーター、及びその用途 |
| EP2793936A4 (en) * | 2011-12-19 | 2015-05-27 | Lfb Usa Inc | RECOMBINANT HUMAN ALPHA-1-ANTITRYPINE FOR THE TREATMENT OF INFLAMMATORY DISEASES |
| CL2014002410A1 (es) * | 2014-09-12 | 2014-11-14 | Univ Concepcion | Método para la producción de proteínas recombinantes en glándula mamaria de mamíferos mediante la transformación del epítelio glandular mamario con vectores adenoasociados. |
| WO2018085967A1 (en) * | 2016-11-08 | 2018-05-17 | Guangzhou Institutes Of Biomedicine And Health, Chinese Academy Of Sciences | Genetically humanized mammals expressing human serum albumin and uses thereof |
| WO2021013997A1 (en) | 2019-07-25 | 2021-01-28 | Medichem, S.A. | Process for the synthesis of n-alkyl-4-pyridinamines |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5780009A (en) * | 1995-01-20 | 1998-07-14 | Nexia Biotechnologies, Inc. | Direct gene transfer into the ruminant mammary gland |
| WO1997017090A1 (en) * | 1995-11-07 | 1997-05-15 | Baylor College Of Medicine | Adenovirus-mediated production of bioactive proteins by mammalian cells and animals |
| CA2258755A1 (en) * | 1996-06-20 | 1997-12-24 | Merck & Co., Inc. | Gene therapy for obesity |
| WO1999043795A1 (en) * | 1998-02-24 | 1999-09-02 | Her Majesty In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada | Trans-somatics with gene transfer into mammary epithelial cells |
| AU2002228730A1 (en) * | 2000-10-27 | 2002-05-06 | Beth Israel Deaconess Medical Center, Inc. | Mammary secreted protein 36 (msp36) for cancer treatment and diagnosis |
-
2002
- 2002-10-21 CU CU20020235A patent/CU23102A1/es unknown
-
2003
- 2003-10-16 AR ARP030103775A patent/AR041644A1/es not_active Application Discontinuation
- 2003-10-20 KR KR1020057006830A patent/KR20050083791A/ko not_active Application Discontinuation
- 2003-10-20 JP JP2004543918A patent/JP2006503560A/ja active Pending
- 2003-10-20 CN CNA2003801034448A patent/CN1744814A/zh active Pending
- 2003-10-20 RU RU2005115495/13A patent/RU2345088C2/ru active
- 2003-10-20 EP EP03757653A patent/EP1557084A2/en not_active Withdrawn
- 2003-10-20 CA CA002502902A patent/CA2502902A1/en not_active Abandoned
- 2003-10-20 MX MXPA05004295A patent/MXPA05004295A/es unknown
- 2003-10-20 BR BR0315555-2A patent/BR0315555A/pt not_active IP Right Cessation
- 2003-10-20 WO PCT/CU2003/000011 patent/WO2004034780A2/es active Application Filing
- 2003-10-20 AU AU2003273726A patent/AU2003273726B8/en not_active Expired - Fee Related
-
2005
- 2005-04-19 ZA ZA200503166A patent/ZA200503166B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AR041644A1 (es) | 2005-05-26 |
| AU2003273726B2 (en) | 2009-07-09 |
| AU2003273726A1 (en) | 2004-05-04 |
| ZA200503166B (en) | 2006-10-25 |
| CU23102A1 (es) | 2005-12-20 |
| AU2003273726B8 (en) | 2009-09-03 |
| CN1744814A (zh) | 2006-03-08 |
| KR20050083791A (ko) | 2005-08-26 |
| RU2345088C2 (ru) | 2009-01-27 |
| WO2004034780A2 (es) | 2004-04-29 |
| WO2004034780A3 (es) | 2004-05-27 |
| EP1557084A2 (en) | 2005-07-27 |
| MXPA05004295A (es) | 2005-08-03 |
| CA2502902A1 (en) | 2004-04-29 |
| BR0315555A (pt) | 2005-08-23 |
| JP2006503560A (ja) | 2006-02-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10441634B2 (en) | Treatment of peripheral vascular disease using protein solutions | |
| US11957733B2 (en) | Treatment of collagen defects using protein solutions | |
| US20180243376A1 (en) | Methods and non-immunogenic compositions for treating inflammatory disorders | |
| US9833474B2 (en) | Methods of mediating macrophage phenotypes | |
| US10143725B2 (en) | Treatment of pain using protein solutions | |
| CN112851797B (zh) | 一种重组人iii型胶原蛋白及其制备方法和用途 | |
| RU2005115495A (ru) | Способ получения рекомбинантных белков в молочной железе не трансгенных млекопитающих | |
| EP2968409A1 (en) | Treatment of inflammatory respiratory disease using biological solutions | |
| US20160074479A1 (en) | Methods of treating spinal disorders using autologous protein solutions | |
| CN102076351A (zh) | 损伤组织的功能性再生促进药物 | |
| CN114057854B (zh) | 一种幽门螺杆菌cd4+t细胞耐受多肽融合抗原及其应用 | |
| CN114686502A (zh) | 一种在Raw264.7细胞中快速构建稳定高表达细胞株的方法 | |
| CN117625542A (zh) | 一种用于调节nk细胞数量的msc滋养层细胞及其制备方法和应用 | |
| CN1478898A (zh) | 一种通用的抗体靶向非病毒型基因导入系统 |