AR020332A1 - Metodo para incrementar la produccion de una proteina en una celula de planta y una construccion - Google Patents

Metodo para incrementar la produccion de una proteina en una celula de planta y una construccion

Info

Publication number
AR020332A1
AR020332A1 ARP990103392A ARP990103392A AR020332A1 AR 020332 A1 AR020332 A1 AR 020332A1 AR P990103392 A ARP990103392 A AR P990103392A AR P990103392 A ARP990103392 A AR P990103392A AR 020332 A1 AR020332 A1 AR 020332A1
Authority
AR
Argentina
Prior art keywords
protein
production
plant cell
plastid
interest
Prior art date
Application number
ARP990103392A
Other languages
English (en)
Original Assignee
Calgene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calgene Inc filed Critical Calgene Inc
Publication of AR020332A1 publication Critical patent/AR020332A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8114—Kunitz type inhibitors
    • C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un método para incrementar la produccion de una proteína en una célula de planta, que comprende transformar plástidos de dicha célula de planta con unaconstruccion que comprende lo siguiente como componentes operablemente unidos en la direccion 5 a 3de transcripcion: a) un promotor funcional en un plástidode planta; b) un sitio de enlace a ribosoma; c) una secuencia de ADN de interés; y d) una region de terminacion de transcripcion; y desarrollar células deplantas que comprenden dichos plástidos transformados, bajo condiciones en donde dicha secuencia codificadora de ADN sea transcripta en dicho plástido. Métodospara incrementar la produccion de proteína fusionando una secuencia que codifica para un gen de interés, a una proteína secundaria, para el marcado o laruptura de la proteína de interés dentro del plástido, por lo cual se logran altos niveles de expresion.
ARP990103392A 1998-07-10 1999-07-12 Metodo para incrementar la produccion de una proteina en una celula de planta y una construccion AR020332A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/113,690 US6271444B1 (en) 1998-07-10 1998-07-10 Enhancer elements for increased translation in plant plastids

Publications (1)

Publication Number Publication Date
AR020332A1 true AR020332A1 (es) 2002-05-08

Family

ID=22350932

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP990103392A AR020332A1 (es) 1998-07-10 1999-07-12 Metodo para incrementar la produccion de una proteina en una celula de planta y una construccion

Country Status (9)

Country Link
US (1) US6271444B1 (es)
EP (1) EP1095144B8 (es)
JP (1) JP2002520022A (es)
AR (1) AR020332A1 (es)
AT (1) ATE443140T1 (es)
CA (1) CA2333308A1 (es)
DE (1) DE69941432D1 (es)
MX (1) MXPA01000366A (es)
WO (1) WO2000003017A2 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE44266E1 (en) 1997-01-17 2013-06-04 The Scripps Research Institute Expression of eukaryotic polypeptides in chloroplasts
US6987215B1 (en) * 1998-08-03 2006-01-17 Rutgers, The State University Of New Jersey Translation control elements for high-level protein expression in the plastids of higher plants and methods of use thereof
WO2001007590A2 (en) * 1999-07-27 2001-02-01 Syngenta Participations Ag Chimeric genes for plastid expression
US20040253586A1 (en) * 1999-12-08 2004-12-16 Siva Reddy Plastid transformation
US20020137214A1 (en) * 2001-04-18 2002-09-26 Henry Daniell Marker free transgenic plants engineering the chloroplast genome without the use of antibiotic selection
GB2364705A (en) * 2000-07-14 2002-02-06 Icgeb Fusion protein expression in plastids
CA2483337C (en) * 2002-04-23 2015-10-27 The Scripps Research Institute Expression of polypeptides in chloroplasts, and compositions and methods for expressing same
CA2491639A1 (en) * 2002-07-03 2004-01-15 University Of Central Florida Expression of the human igf-1 in transgenic plastids
CA2530994A1 (en) * 2003-07-01 2005-01-20 Biolex, Inc. Chloroplast transformation of duckweed
EP1720989B1 (en) * 2004-03-03 2008-08-13 National University Corporation Nara Institute of Science and Technology Method for improving productivity of plant by chloroplast technology
US20090007301A1 (en) 2005-04-15 2009-01-01 Hsu-Ching Chen Wintz Plant Promoters, Terminators, Genes, Vectors and Related Transformed Plants
US20070037230A1 (en) * 2005-04-29 2007-02-15 Petrenko Valery A Peptides for selectively and specifically binding Bacillus anthracis spores and use thereof in landscape phages
SE529377C2 (sv) * 2005-10-18 2007-07-24 Morphic Technologies Ab Publ Metod och arrangemang för att lokalisera och plocka upp föremål från en bärare
CA2681661C (en) 2007-03-23 2015-11-24 New York University Methods of increasing nitrogen-assimilation capacity in transgenic plants expressing cca1 and glk1
KR20130020842A (ko) 2007-06-01 2013-02-28 더 스크립스 리서치 인스티튜트 유전적으로 변형된 광합성 생물들의 고처리량 스크리닝
MX2010002723A (es) * 2007-09-11 2010-05-21 Sapphire Energy Inc Produccion de moleculas mediante organismos fotosinteticos.
KR101449155B1 (ko) * 2012-12-06 2014-10-13 주식회사 바이오앱 식물체에서 목적 단백질의 번역 효율을 증진시키기 위한 염기서열

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Publication number Priority date Publication date Assignee Title
US4342832A (en) 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
FI864720A (fi) 1985-11-22 1987-05-23 Ciba Geigy Ag Direkt gentransmission i plasticider och mitokondrier.
IL82017A (en) 1986-03-27 1996-01-19 Monsanto Co Increased production of protein in bacteria by using a ribosomal linker site based on bacteriophage T7 gene 01
US5232840A (en) 1986-03-27 1993-08-03 Monsanto Company Enhanced protein production in bacteria by employing a novel ribosome binding site
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
US4971908A (en) * 1987-05-26 1990-11-20 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthase
US5416011A (en) 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
US5693507A (en) * 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
ES2150900T3 (es) 1989-10-31 2000-12-16 Monsanto Co Promotor para plantas transgenicas.
US5877402A (en) * 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
WO1992004449A1 (en) * 1990-08-31 1992-03-19 Monsanto Company Glyphosate tolerant 5-enolpyruvylshikimate-3-phosphate synthases
WO1992015675A1 (en) 1991-03-06 1992-09-17 Agracetus, Inc. Particle mediated transformation of cotton
US5576198A (en) * 1993-12-14 1996-11-19 Calgene, Inc. Controlled expression of transgenic constructs in plant plastids
US5545817A (en) * 1994-03-11 1996-08-13 Calgene, Inc. Enhanced expression in a plant plastid
EP0925362B1 (en) * 1996-09-12 2005-07-20 Syngenta Participations AG Transgenic plants expressing cellulolytic enzymes

Also Published As

Publication number Publication date
EP1095144B8 (en) 2009-11-18
CA2333308A1 (en) 2000-01-20
JP2002520022A (ja) 2002-07-09
MXPA01000366A (es) 2002-10-17
US6271444B1 (en) 2001-08-07
WO2000003017A3 (en) 2000-07-06
WO2000003017A2 (en) 2000-01-20
DE69941432D1 (de) 2009-10-29
EP1095144A2 (en) 2001-05-02
EP1095144B1 (en) 2009-09-16
ATE443140T1 (de) 2009-10-15

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