AR014791A1 - Fragmento aislado de acidos nucleicos que codifica toda o una porcion substancial de una enzima involucrada enla biosintesis del triptofano ensemillas y planta, gen quimerico, celula huesped transformada, polipeptido, metodo para alterar el nivel de expresion de dicha enzima, metodo para obtenerdich - Google Patents

Fragmento aislado de acidos nucleicos que codifica toda o una porcion substancial de una enzima involucrada enla biosintesis del triptofano ensemillas y planta, gen quimerico, celula huesped transformada, polipeptido, metodo para alterar el nivel de expresion de dicha enzima, metodo para obtenerdich

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Publication number
AR014791A1
AR014791A1 ARP990101382A ARP990101382A AR014791A1 AR 014791 A1 AR014791 A1 AR 014791A1 AR P990101382 A ARP990101382 A AR P990101382A AR P990101382 A ARP990101382 A AR P990101382A AR 014791 A1 AR014791 A1 AR 014791A1
Authority
AR
Argentina
Prior art keywords
nucleic acids
biosynthesis
enzymes
exodium
substancial
Prior art date
Application number
ARP990101382A
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English (en)
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of AR014791A1 publication Critical patent/AR014791A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/227—Tryptophan

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se definen en esta descripcion fragmentos aislados de ácidos nucleicos que codifican enzimas para la biosíntesis de triptofano y porciones de ellas. Estasenzimas son la alfa antranilato sintasa y subunidades alfa y beta de la triptofano sintasa. Asimismo se incluyen genes quiméricos conteniendo dichosfragmentos de ácidos nucleicos y células huésped transformadas por dichos genes quiméricos. Los polipéptidos codificados por dichos fragmentos de ácidosnucleicos y un método para alterar el nivel de expresion de las enzimas de la biosíntesis del triptofano, también se incluyen. Se describen métodos deobtencion de polinucleotidos que codifican enzimas de la biosíntesis de triptofano y los productos que se obtienen por estos métodos. Finalmente sedetalla un método para evaluar la capacidad de un compuesto para inhibir la actividad de dichas enzimas.
ARP990101382A 1998-03-26 1999-03-26 Fragmento aislado de acidos nucleicos que codifica toda o una porcion substancial de una enzima involucrada enla biosintesis del triptofano ensemillas y planta, gen quimerico, celula huesped transformada, polipeptido, metodo para alterar el nivel de expresion de dicha enzima, metodo para obtenerdich AR014791A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US7938698P 1998-03-26 1998-03-26

Publications (1)

Publication Number Publication Date
AR014791A1 true AR014791A1 (es) 2001-03-28

Family

ID=22150226

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP990101382A AR014791A1 (es) 1998-03-26 1999-03-26 Fragmento aislado de acidos nucleicos que codifica toda o una porcion substancial de una enzima involucrada enla biosintesis del triptofano ensemillas y planta, gen quimerico, celula huesped transformada, polipeptido, metodo para alterar el nivel de expresion de dicha enzima, metodo para obtenerdich

Country Status (6)

Country Link
US (1) US6482631B1 (es)
EP (1) EP1071793A2 (es)
AR (1) AR014791A1 (es)
AU (2) AU3103499A (es)
BR (1) BR9908101A (es)
WO (2) WO1999049058A2 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999011800A1 (en) 1997-08-29 1999-03-11 Hokko Chemical Industry Co., Ltd. GENE ENCODING α-SUBUNIT OF RICE ANTHRANILATE SYNTHASE AND DNA RELATING THERETO
EP1144672A3 (en) * 1999-02-05 2002-03-27 American Cyanamid Company Tryptophan synthase as a site of herbicide action
CA2402829A1 (en) * 2000-03-24 2001-10-04 Pioneer Hi-Bred International, Inc. Methods of selection and development of plants having improved root quality and root lodging resistance
EP2216405A1 (en) 2002-05-03 2010-08-11 Monsanto Technology LLC Speed specific USP promoters for expressing genes in plants
US7078234B2 (en) 2002-12-18 2006-07-18 Monsanto Technology Llc Maize embryo-specific promoter compositions and methods for use thereof
EP2116606B1 (en) 2003-03-28 2012-12-19 Monsanto Technology, LLC Novel plant promoters for use in early seed development
WO2006089950A2 (en) 2005-02-26 2006-08-31 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
CA2606220A1 (en) 2005-04-19 2006-12-21 Basf Plant Science Gmbh Starchy-endosperm and/or germinating embryo-specific expression in mono-cotyledonous plants
BRPI0614070A2 (pt) 2005-05-10 2018-07-31 Basf Plant Science Gmbh cassetes de expressão, sequência de nucleotídeos isolada, seqüência sintética reguladora de transcrição, métodos para proporcionar uma seqüência sintética de nucleotídeos reguladora de transcrição, e um cassete de expressão, vetor, organismo não-humano ou célula hospedeira transgênico(a), e, célula de planta ou planta transgênica
CA2675926A1 (en) 2007-02-16 2008-08-21 Basf Plant Science Gmbh Nucleic acid sequences for regulation of embryo-specific expression in monocotyledonous plants
US20120036593A1 (en) 2009-04-17 2012-02-09 Basf Plant Science Company Gmbh Plant Promoter Operable in Endosperm and Uses Thereof
WO2011003901A1 (en) 2009-07-10 2011-01-13 Basf Plant Science Company Gmbh Expression cassettes for endosperm-specific expression in plants
CN102782141A (zh) 2009-12-03 2012-11-14 巴斯夫植物科学有限公司 用于在植物中胚-特异性表达的表达盒
CN105143440B (zh) * 2013-04-16 2019-06-14 Cj第一制糖株式会社 具有l-色氨酸生产力的微生物以及使用所述微生物生产l-色氨酸的方法
EP3404036A1 (en) 2017-05-19 2018-11-21 Enzypep B.V. Subtilisin variants having a mutation in the s2 or s2' pocket

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6118047A (en) * 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
WO1999011800A1 (en) * 1997-08-29 1999-03-11 Hokko Chemical Industry Co., Ltd. GENE ENCODING α-SUBUNIT OF RICE ANTHRANILATE SYNTHASE AND DNA RELATING THERETO

Also Published As

Publication number Publication date
WO1999049013A3 (en) 2002-04-25
AU3103499A (en) 1999-10-18
BR9908101A (pt) 2000-12-12
AU3115899A (en) 1999-10-18
WO1999049058A3 (en) 2000-04-06
WO1999049058A2 (en) 1999-09-30
WO1999049013A2 (en) 1999-09-30
EP1071793A2 (en) 2001-01-31
US6482631B1 (en) 2002-11-19

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