AR015734A1 - Fragmento aislado de acidos nucleicos que codifica una enzima de la biosintesis de los lipidos, gen quimerico, celula huesped, polipeptido, metodo paraalterar el nivel de expresion de dichas enzimas, metodo para producir un acido graso insaturado, semilla, aceite, metodo para producir aceite, metodo - Google Patents
Fragmento aislado de acidos nucleicos que codifica una enzima de la biosintesis de los lipidos, gen quimerico, celula huesped, polipeptido, metodo paraalterar el nivel de expresion de dichas enzimas, metodo para producir un acido graso insaturado, semilla, aceite, metodo para producir aceite, metodoInfo
- Publication number
- AR015734A1 AR015734A1 ARP990101226A ARP990101226A AR015734A1 AR 015734 A1 AR015734 A1 AR 015734A1 AR P990101226 A ARP990101226 A AR P990101226A AR P990101226 A ARP990101226 A AR P990101226A AR 015734 A1 AR015734 A1 AR 015734A1
- Authority
- AR
- Argentina
- Prior art keywords
- acyl
- oils
- delta
- host cell
- level
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0083—Miscellaneous (1.14.99)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Nutrition Science (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Fats And Perfumes (AREA)
Abstract
Se describen fragmentos de ácidos nucleicos aislados que codifican enzimas biosintéticas de aceites de Limnanthes. Específicamente, un fragmento aislado deácidos nucleicos que codifica una delta-5 acil-CoA desaturasa o una acilo graso-CoA elongasa ysu complementario. Una realizacion adicional se relaciona con unpolipéptido que codifica toda o una porcion sustancial de dicha enzima y un gen quimérico que dicho fragmento, ligado operativamente a secuencias reguladorasadecuadas, donde laexpresion del gen quimérico da como resultado la produccion de la proteína codificada en una célula huésped transformada, que también sedescribe. Una realizacion adicional de la presente invencion se refiere a un método para alterar el nivel de unadelta-5 ac il-CoA desaturasa o una acilograso-CoA elongasa en una célula huésped transformada, un método para obtener un fragmento de ácido nucleico que codifica toda o una porcion sustancial de unasecuencia de aminoácidos codificadora de unadelta-5 acil-CoA d esaturasa o una acilo graso-CoA elongasa y los productos que se obtienen,un método para producirun ácido graso desaturado que contenga un enlace doble en la posicion delta-5 en una célula huésped, y semillas, aceites y métodospara producir aceites d esemillas, donde dichas semillas y aceites contienen ácidos grasos desaturados que a su vez comprenden un enlace doble en la posicion delta-5. Otra realizacionadicional es un método para reducir el nivel de ácidos grasos de16 carbonos en una célula huésped y un método para incrementar el nivel de ácidos grasos de 20carbonos en una célula huésped, y semillas, aceites y métodos para producir aceites de semillas con niveles reducidos de ácidos grasos de 16 carbonos onivelesincrementados de ácidos grasos de 20 carbonos.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US7873698P | 1998-03-20 | 1998-03-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR015734A1 true AR015734A1 (es) | 2001-05-16 |
Family
ID=22145915
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP990101226A AR015734A1 (es) | 1998-03-20 | 1999-03-19 | Fragmento aislado de acidos nucleicos que codifica una enzima de la biosintesis de los lipidos, gen quimerico, celula huesped, polipeptido, metodo paraalterar el nivel de expresion de dichas enzimas, metodo para producir un acido graso insaturado, semilla, aceite, metodo para producir aceite, metodo |
Country Status (14)
| Country | Link |
|---|---|
| EP (3) | EP2006385A3 (es) |
| JP (1) | JP2002528048A (es) |
| KR (1) | KR20010042015A (es) |
| AR (1) | AR015734A1 (es) |
| AU (1) | AU763296B2 (es) |
| BR (1) | BR9908381A (es) |
| CA (2) | CA2319727C (es) |
| DE (1) | DE69938961D1 (es) |
| ES (1) | ES2310033T3 (es) |
| HU (1) | HUP0102912A3 (es) |
| NO (1) | NO20004680L (es) |
| NZ (1) | NZ505988A (es) |
| PT (1) | PT1064387E (es) |
| WO (1) | WO1999049050A2 (es) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19950589A1 (de) * | 1999-10-20 | 2001-05-23 | Gvs Ges Fuer Erwerb Und Verwer | Elongasepromotoren für gewebespezifische Expression von Transgenen in Pflanzen |
| CN102559364B (zh) | 2004-04-22 | 2016-08-17 | 联邦科学技术研究组织 | 用重组细胞合成长链多不饱和脂肪酸 |
| CA2563875C (en) * | 2004-04-22 | 2015-06-30 | Commonwealth Scientific And Industrial Research Organisation | Synthesis of long-chain polyunsaturated fatty acids by recombinant cells |
| EP1910545A2 (en) * | 2005-07-25 | 2008-04-16 | BASF Plant Science GmbH | Combination of lipid metabolism proteins and uses thereof |
| US7723574B2 (en) * | 2005-11-24 | 2010-05-25 | Basf Plant Science Gmbh | Process for the production of Δ5-unsaturated fatty acids in transgenic organisms |
| CA2693630C (en) | 2006-07-14 | 2021-08-31 | Commonwealth Scientific And Industrial Research Organisation | Altering the fatty acid composition of rice |
| RU2009111266A (ru) | 2006-08-29 | 2010-10-10 | Коммонвелт Сайентифик энд Индастриал Рисерч Организейшн (AU) | Синтез жирных кислот |
| AU2009273755A1 (en) | 2008-07-21 | 2010-01-28 | Commonwealth Scientific And Industrial Research Organisation | Improved vegetable oils and uses therefor |
| AU2009273754B2 (en) | 2008-07-21 | 2016-07-14 | Commonwealth Scientific And Industrial Research Organisation | Improved cottonseed oil and uses |
| EP2358882B1 (en) | 2008-11-18 | 2017-07-26 | Commonwealth Scientific and Industrial Research Organisation | Enzymes and methods for producing omega-3 fatty acids |
| WO2012000026A1 (en) | 2010-06-28 | 2012-01-05 | Commonwealth Scientific And Industrial Research Organisation | Methods of producing lipids |
| CN104271743B (zh) | 2011-12-27 | 2019-11-05 | 联邦科学技术研究组织 | 产生脂质的方法 |
| US11639507B2 (en) | 2011-12-27 | 2023-05-02 | Commonwealth Scientific And Industrial Research Organisation | Processes for producing lipids |
| US8809026B2 (en) | 2011-12-27 | 2014-08-19 | Commonwealth Scientific And Industrial Research Organisation | Processes for producing lipids |
| WO2013159149A1 (en) | 2012-04-25 | 2013-10-31 | Commonwealth Scientific And Industrial Research Organisation | High oleic acid oils |
| EP2861059B1 (en) | 2012-06-15 | 2017-05-03 | Commonwealth Scientific and Industrial Research Organisation | Production of long chain polyunsaturated fatty acids in plant cells |
| NZ721036A (en) | 2013-12-18 | 2023-07-28 | Grains Res & Dev Corp | Lipid comprising long chain polyunsaturated fatty acids |
| CN105219789B (zh) | 2014-06-27 | 2023-04-07 | 联邦科学技术研究组织 | 包含二十二碳五烯酸的提取的植物脂质 |
| JP6882160B2 (ja) | 2014-07-07 | 2021-06-02 | コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガナイゼーション | 植物脂質から工業製品を製造する工程 |
| BR112019004530A2 (pt) | 2016-09-02 | 2019-10-22 | Commw Scient Ind Res Org | plantas com traços modificados |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
| EP0242236B2 (en) | 1986-03-11 | 1996-08-21 | Plant Genetic Systems N.V. | Plant cells resistant to glutamine synthetase inhibitors, made by genetic engineering |
| US5107065A (en) | 1986-03-28 | 1992-04-21 | Calgene, Inc. | Anti-sense regulation of gene expression in plant cells |
| US5231020A (en) | 1989-03-30 | 1993-07-27 | Dna Plant Technology Corporation | Genetic engineering of novel plant phenotypes |
| US5679881A (en) * | 1991-11-20 | 1997-10-21 | Calgene, Inc. | Nucleic acid sequences encoding a plant cytoplasmic protein involved in fatty acyl-CoA metabolism |
| CA2123893A1 (en) * | 1991-11-20 | 1993-05-27 | James G. Metz | Fatty acyl-coa:fatty alcohol o-acyltransferases |
| DK0616644T3 (da) * | 1991-12-04 | 2003-10-27 | Du Pont | Fedtsyredesaturase-gener fra planter |
| DK0668919T3 (da) * | 1992-11-17 | 2003-09-15 | Du Pont | Gener for mikorsomale delta-12-fedtsyredesaturaser og beslægtede enzymer fra planter |
| DE4433307A1 (de) * | 1994-09-19 | 1996-03-21 | Norddeutsche Pflanzenzucht Han | Ein isoliertes Nuckleinsäurefragment und daraus abgeleitete Produkte |
| GB9502468D0 (en) * | 1995-02-09 | 1995-03-29 | Gene Shears Pty Ltd | DNA Sequence |
| EP0996732B1 (en) * | 1997-04-11 | 2005-06-15 | Calgene LLC | Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants |
| EP1033405A3 (en) * | 1999-02-25 | 2001-08-01 | Ceres Incorporated | Sequence-determined DNA fragments and corresponding polypeptides encoded thereby |
| US7325049B2 (en) | 2000-12-29 | 2008-01-29 | Intel Corporation | Alert management messaging |
| EP2337791B1 (en) * | 2008-10-14 | 2013-07-24 | Monsanto Technology LLC | Utilization of fatty acid desaturases from hemiselmis spp |
-
1999
- 1999-03-12 AU AU31849/99A patent/AU763296B2/en not_active Ceased
- 1999-03-12 EP EP08006579A patent/EP2006385A3/en not_active Withdrawn
- 1999-03-12 DE DE69938961T patent/DE69938961D1/de not_active Expired - Lifetime
- 1999-03-12 EP EP20100013147 patent/EP2316949B1/en not_active Expired - Lifetime
- 1999-03-12 BR BR9908381-7A patent/BR9908381A/pt not_active Application Discontinuation
- 1999-03-12 EP EP99913868A patent/EP1064387B1/en not_active Expired - Lifetime
- 1999-03-12 KR KR1020007010348A patent/KR20010042015A/ko not_active Application Discontinuation
- 1999-03-12 WO PCT/US1999/005471 patent/WO1999049050A2/en not_active Application Discontinuation
- 1999-03-12 PT PT99913868T patent/PT1064387E/pt unknown
- 1999-03-12 NZ NZ505988A patent/NZ505988A/en not_active IP Right Cessation
- 1999-03-12 HU HU0102912A patent/HUP0102912A3/hu unknown
- 1999-03-12 JP JP2000538011A patent/JP2002528048A/ja active Pending
- 1999-03-12 CA CA2319727A patent/CA2319727C/en not_active Expired - Fee Related
- 1999-03-12 CA CA2783215A patent/CA2783215C/en not_active Expired - Fee Related
- 1999-03-12 ES ES99913868T patent/ES2310033T3/es not_active Expired - Lifetime
- 1999-03-19 AR ARP990101226A patent/AR015734A1/es not_active Application Discontinuation
-
2000
- 2000-09-19 NO NO20004680A patent/NO20004680L/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| NO20004680D0 (no) | 2000-09-19 |
| NO20004680L (no) | 2000-11-15 |
| AU3184999A (en) | 1999-10-18 |
| JP2002528048A (ja) | 2002-09-03 |
| HUP0102912A3 (en) | 2003-07-28 |
| ES2310033T3 (es) | 2008-12-16 |
| EP1064387A2 (en) | 2001-01-03 |
| CA2783215C (en) | 2015-11-17 |
| NZ505988A (en) | 2003-10-31 |
| EP2006385A3 (en) | 2008-12-31 |
| HUP0102912A2 (hu) | 2001-11-28 |
| EP2316949A1 (en) | 2011-05-04 |
| CA2783215A1 (en) | 1999-09-30 |
| EP2006385A2 (en) | 2008-12-24 |
| DE69938961D1 (de) | 2008-08-07 |
| WO1999049050A3 (en) | 1999-12-29 |
| WO1999049050A2 (en) | 1999-09-30 |
| CA2319727C (en) | 2012-09-25 |
| CA2319727A1 (en) | 1999-09-30 |
| KR20010042015A (ko) | 2001-05-25 |
| AU763296B2 (en) | 2003-07-17 |
| BR9908381A (pt) | 2002-06-11 |
| EP2316949B1 (en) | 2015-04-22 |
| EP1064387B1 (en) | 2008-06-25 |
| PT1064387E (pt) | 2008-09-15 |
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| FA | Abandonment or withdrawal |