AR015734A1 - Fragmento aislado de acidos nucleicos que codifica una enzima de la biosintesis de los lipidos, gen quimerico, celula huesped, polipeptido, metodo paraalterar el nivel de expresion de dichas enzimas, metodo para producir un acido graso insaturado, semilla, aceite, metodo para producir aceite, metodo - Google Patents
Fragmento aislado de acidos nucleicos que codifica una enzima de la biosintesis de los lipidos, gen quimerico, celula huesped, polipeptido, metodo paraalterar el nivel de expresion de dichas enzimas, metodo para producir un acido graso insaturado, semilla, aceite, metodo para producir aceite, metodoInfo
- Publication number
- AR015734A1 AR015734A1 ARP990101226A ARP990101226A AR015734A1 AR 015734 A1 AR015734 A1 AR 015734A1 AR P990101226 A ARP990101226 A AR P990101226A AR P990101226 A ARP990101226 A AR P990101226A AR 015734 A1 AR015734 A1 AR 015734A1
- Authority
- AR
- Argentina
- Prior art keywords
- acyl
- oils
- delta
- host cell
- level
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0083—Miscellaneous (1.14.99)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Nutrition Science (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Fats And Perfumes (AREA)
Abstract
Se describen fragmentos de ácidos nucleicos aislados que codifican enzimas biosintéticas de aceites de Limnanthes. Específicamente, un fragmento aislado deácidos nucleicos que codifica una delta-5 acil-CoA desaturasa o una acilo graso-CoA elongasa ysu complementario. Una realizacion adicional se relaciona con unpolipéptido que codifica toda o una porcion sustancial de dicha enzima y un gen quimérico que dicho fragmento, ligado operativamente a secuencias reguladorasadecuadas, donde laexpresion del gen quimérico da como resultado la produccion de la proteína codificada en una célula huésped transformada, que también sedescribe. Una realizacion adicional de la presente invencion se refiere a un método para alterar el nivel de unadelta-5 ac il-CoA desaturasa o una acilograso-CoA elongasa en una célula huésped transformada, un método para obtener un fragmento de ácido nucleico que codifica toda o una porcion sustancial de unasecuencia de aminoácidos codificadora de unadelta-5 acil-CoA d esaturasa o una acilo graso-CoA elongasa y los productos que se obtienen,un método para producirun ácido graso desaturado que contenga un enlace doble en la posicion delta-5 en una célula huésped, y semillas, aceites y métodospara producir aceites d esemillas, donde dichas semillas y aceites contienen ácidos grasos desaturados que a su vez comprenden un enlace doble en la posicion delta-5. Otra realizacionadicional es un método para reducir el nivel de ácidos grasos de16 carbonos en una célula huésped y un método para incrementar el nivel de ácidos grasos de 20carbonos en una célula huésped, y semillas, aceites y métodos para producir aceites de semillas con niveles reducidos de ácidos grasos de 16 carbonos onivelesincrementados de ácidos grasos de 20 carbonos.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US7873698P | 1998-03-20 | 1998-03-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR015734A1 true AR015734A1 (es) | 2001-05-16 |
Family
ID=22145915
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP990101226A AR015734A1 (es) | 1998-03-20 | 1999-03-19 | Fragmento aislado de acidos nucleicos que codifica una enzima de la biosintesis de los lipidos, gen quimerico, celula huesped, polipeptido, metodo paraalterar el nivel de expresion de dichas enzimas, metodo para producir un acido graso insaturado, semilla, aceite, metodo para producir aceite, metodo |
Country Status (14)
| Country | Link |
|---|---|
| EP (3) | EP1064387B1 (es) |
| JP (1) | JP2002528048A (es) |
| KR (1) | KR20010042015A (es) |
| AR (1) | AR015734A1 (es) |
| AU (1) | AU763296B2 (es) |
| BR (1) | BR9908381A (es) |
| CA (2) | CA2319727C (es) |
| DE (1) | DE69938961D1 (es) |
| ES (1) | ES2310033T3 (es) |
| HU (1) | HUP0102912A3 (es) |
| NO (1) | NO20004680L (es) |
| NZ (1) | NZ505988A (es) |
| PT (1) | PT1064387E (es) |
| WO (1) | WO1999049050A2 (es) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19950589A1 (de) * | 1999-10-20 | 2001-05-23 | Gvs Ges Fuer Erwerb Und Verwer | Elongasepromotoren für gewebespezifische Expression von Transgenen in Pflanzen |
| CN102559364B (zh) | 2004-04-22 | 2016-08-17 | 联邦科学技术研究组织 | 用重组细胞合成长链多不饱和脂肪酸 |
| BRPI0510132A (pt) | 2004-04-22 | 2007-10-02 | Commw Scient Ind Res Org | sìntese de ácidos graxos poliinsaturados de cadeia longa por células recombinantes |
| CA2615837A1 (en) * | 2005-07-25 | 2007-02-01 | Basf Plant Science Gmbh | Combination of lipid metabolism proteins and uses thereof |
| US7723574B2 (en) | 2005-11-24 | 2010-05-25 | Basf Plant Science Gmbh | Process for the production of Δ5-unsaturated fatty acids in transgenic organisms |
| CN101516181B (zh) | 2006-07-14 | 2015-09-30 | 联邦科学技术研究组织 | 改变水稻的脂肪酸组成 |
| EP2059588A4 (en) | 2006-08-29 | 2010-07-28 | Commw Scient Ind Res Org | FATTY ACID SYNTHESIS |
| CN102202498B (zh) | 2008-07-21 | 2016-09-07 | 澳大利亚联邦科学与工业研究组织 | 改良的棉籽油及应用 |
| WO2010009500A1 (en) | 2008-07-21 | 2010-01-28 | Commonwealth Scientific And Industrial Research Organisation | Improved vegetable oils and uses therefor |
| CN114045301A (zh) | 2008-11-18 | 2022-02-15 | 联邦科学技术研究组织 | 产生ω-3脂肪酸的酶和方法 |
| CN103201379B (zh) | 2010-06-28 | 2015-08-19 | 联邦科学技术研究组织 | 生产脂质的方法 |
| MY196561A (en) | 2011-12-27 | 2023-04-19 | Commw Scient Ind Res Org | Processes for Producing Lipids |
| US11639507B2 (en) | 2011-12-27 | 2023-05-02 | Commonwealth Scientific And Industrial Research Organisation | Processes for producing lipids |
| US8809026B2 (en) | 2011-12-27 | 2014-08-19 | Commonwealth Scientific And Industrial Research Organisation | Processes for producing lipids |
| NZ631696A (en) | 2012-04-25 | 2017-02-24 | Grains Res & Dev Corp | High oleic acid oils |
| US8816111B2 (en) | 2012-06-15 | 2014-08-26 | Commonwealth Scientific And Industrial Research Organisation | Lipid comprising polyunsaturated fatty acids |
| CN104726473B (zh) | 2013-12-18 | 2020-02-14 | 联邦科学技术研究组织 | 包含二十二碳六烯酸的提取的植物脂质 |
| CN105219789B (zh) | 2014-06-27 | 2023-04-07 | 联邦科学技术研究组织 | 包含二十二碳五烯酸的提取的植物脂质 |
| EP4303288A3 (en) | 2014-07-07 | 2024-03-06 | Nuseed Global Innovation Ltd | Processes for producing industrial products from plant lipids |
| CN110462043A (zh) | 2016-09-02 | 2019-11-15 | 联邦科学技术研究组织 | 具有修饰的性状的植物 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
| DE3765449D1 (de) | 1986-03-11 | 1990-11-15 | Plant Genetic Systems Nv | Durch gentechnologie erhaltene und gegen glutaminsynthetase-inhibitoren resistente pflanzenzellen. |
| US5107065A (en) | 1986-03-28 | 1992-04-21 | Calgene, Inc. | Anti-sense regulation of gene expression in plant cells |
| US5231020A (en) | 1989-03-30 | 1993-07-27 | Dna Plant Technology Corporation | Genetic engineering of novel plant phenotypes |
| KR100268752B1 (ko) * | 1991-11-20 | 2000-11-01 | 엘리자베스 라슨 | 지방 아실-CoA : 지방 알코올 O-아실트란스페라제 |
| US5679881A (en) * | 1991-11-20 | 1997-10-21 | Calgene, Inc. | Nucleic acid sequences encoding a plant cytoplasmic protein involved in fatty acyl-CoA metabolism |
| DE69233118T2 (de) | 1991-12-04 | 2004-04-15 | E.I. Du Pont De Nemours And Co., Wilmington | Fettsäure-desaturase gene aus pflanzen |
| DE69333025T2 (de) * | 1992-11-17 | 2004-04-29 | E.I. Du Pont De Nemours And Co., Wilmington | Für mikrosom delta 12 fettsäuredesaturase kodierende gene und verwandte enzyme von pflanzen |
| DE4433307A1 (de) * | 1994-09-19 | 1996-03-21 | Norddeutsche Pflanzenzucht Han | Ein isoliertes Nuckleinsäurefragment und daraus abgeleitete Produkte |
| GB9502468D0 (en) * | 1995-02-09 | 1995-03-29 | Gene Shears Pty Ltd | DNA Sequence |
| JP4087460B2 (ja) * | 1997-04-11 | 2008-05-21 | カルジーン エル エル シー | 植物における長鎖多不飽和脂肪酸の合成のための方法および組成物 |
| EP1033405A3 (en) * | 1999-02-25 | 2001-08-01 | Ceres Incorporated | Sequence-determined DNA fragments and corresponding polypeptides encoded thereby |
| US7325049B2 (en) | 2000-12-29 | 2008-01-29 | Intel Corporation | Alert management messaging |
| DK2337791T3 (da) * | 2008-10-14 | 2013-11-04 | Monsanto Technology Llc | Anvendelse af fedtsyredesaturaser fra Hemiselmis SPP |
-
1999
- 1999-03-12 WO PCT/US1999/005471 patent/WO1999049050A2/en not_active Application Discontinuation
- 1999-03-12 DE DE69938961T patent/DE69938961D1/de not_active Expired - Lifetime
- 1999-03-12 PT PT99913868T patent/PT1064387E/pt unknown
- 1999-03-12 CA CA2319727A patent/CA2319727C/en not_active Expired - Fee Related
- 1999-03-12 EP EP99913868A patent/EP1064387B1/en not_active Expired - Lifetime
- 1999-03-12 AU AU31849/99A patent/AU763296B2/en not_active Ceased
- 1999-03-12 HU HU0102912A patent/HUP0102912A3/hu unknown
- 1999-03-12 JP JP2000538011A patent/JP2002528048A/ja active Pending
- 1999-03-12 BR BR9908381-7A patent/BR9908381A/pt not_active Application Discontinuation
- 1999-03-12 EP EP08006579A patent/EP2006385A3/en not_active Withdrawn
- 1999-03-12 CA CA2783215A patent/CA2783215C/en not_active Expired - Fee Related
- 1999-03-12 ES ES99913868T patent/ES2310033T3/es not_active Expired - Lifetime
- 1999-03-12 KR KR1020007010348A patent/KR20010042015A/ko not_active Application Discontinuation
- 1999-03-12 NZ NZ505988A patent/NZ505988A/en not_active IP Right Cessation
- 1999-03-12 EP EP20100013147 patent/EP2316949B1/en not_active Expired - Lifetime
- 1999-03-19 AR ARP990101226A patent/AR015734A1/es not_active Application Discontinuation
-
2000
- 2000-09-19 NO NO20004680A patent/NO20004680L/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| CA2319727C (en) | 2012-09-25 |
| CA2783215C (en) | 2015-11-17 |
| CA2319727A1 (en) | 1999-09-30 |
| EP2006385A3 (en) | 2008-12-31 |
| BR9908381A (pt) | 2002-06-11 |
| WO1999049050A2 (en) | 1999-09-30 |
| NO20004680D0 (no) | 2000-09-19 |
| WO1999049050A3 (en) | 1999-12-29 |
| EP2316949A1 (en) | 2011-05-04 |
| HUP0102912A2 (hu) | 2001-11-28 |
| AU763296B2 (en) | 2003-07-17 |
| JP2002528048A (ja) | 2002-09-03 |
| EP2316949B1 (en) | 2015-04-22 |
| PT1064387E (pt) | 2008-09-15 |
| EP1064387A2 (en) | 2001-01-03 |
| AU3184999A (en) | 1999-10-18 |
| ES2310033T3 (es) | 2008-12-16 |
| NO20004680L (no) | 2000-11-15 |
| EP2006385A2 (en) | 2008-12-24 |
| KR20010042015A (ko) | 2001-05-25 |
| EP1064387B1 (en) | 2008-06-25 |
| HUP0102912A3 (en) | 2003-07-28 |
| DE69938961D1 (de) | 2008-08-07 |
| CA2783215A1 (en) | 1999-09-30 |
| NZ505988A (en) | 2003-10-31 |
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