AR003776A1 - Metodo para la ruptura de celulas cultivadas empleando un dispositivo de chorro de impacto. - Google Patents

Metodo para la ruptura de celulas cultivadas empleando un dispositivo de chorro de impacto.

Info

Publication number
AR003776A1
AR003776A1 ARP960104582A ARP960104582A AR003776A1 AR 003776 A1 AR003776 A1 AR 003776A1 AR P960104582 A ARP960104582 A AR P960104582A AR P960104582 A ARP960104582 A AR P960104582A AR 003776 A1 AR003776 A1 AR 003776A1
Authority
AR
Argentina
Prior art keywords
jet device
rupture
cells
impact jet
cultivated cells
Prior art date
Application number
ARP960104582A
Other languages
English (en)
Original Assignee
Merck & Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9603759.3A external-priority patent/GB9603759D0/en
Application filed by Merck & Co Inc filed Critical Merck & Co Inc
Publication of AR003776A1 publication Critical patent/AR003776A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16711—Varicellovirus, e.g. human herpesvirus 3, Varicella Zoster, pseudorabies
    • C12N2710/16751—Methods of production or purification of viral material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un novedoso método para fracturar células que no tienen pared celular comprende pasar células suspendidas a través de un dispositivo de chorros deimpacto a baja presión. Este método fractura las células, pero no dana los productos celulares liberados.
ARP960104582A 1995-10-06 1996-10-02 Metodo para la ruptura de celulas cultivadas empleando un dispositivo de chorro de impacto. AR003776A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US506295P 1995-10-06 1995-10-06
GBGB9603759.3A GB9603759D0 (en) 1996-02-22 1996-02-22 Method of disrupting cultured cells using an impinging jet device

Publications (1)

Publication Number Publication Date
AR003776A1 true AR003776A1 (es) 1998-09-09

Family

ID=26308786

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP960104582A AR003776A1 (es) 1995-10-06 1996-10-02 Metodo para la ruptura de celulas cultivadas empleando un dispositivo de chorro de impacto.

Country Status (10)

Country Link
EP (1) EP0854913A1 (es)
CN (1) CN1100868C (es)
AR (1) AR003776A1 (es)
AU (1) AU7204496A (es)
BR (1) BR9610773A (es)
CZ (1) CZ104198A3 (es)
EA (1) EA000703B1 (es)
SK (1) SK43498A3 (es)
TW (1) TW426733B (es)
WO (1) WO1997012959A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19749735C1 (de) * 1997-11-11 2000-02-10 Invent Gmbh Entwicklung Neuer Technologien Verfahren und Vorrichtung zur Herstellung von Enzymen
CA2467099C (en) 2001-12-20 2013-01-29 Bavarian Nordic A/S Method for the recovery and purification of poxviruses from infected cells
DE102005034629B4 (de) * 2005-07-19 2007-09-13 Dr. Hielscher Gmbh Vorrichtung und Verfahren zum mechanischen Aufschluss von Zellen
WO2008019964A1 (de) * 2006-08-15 2008-02-21 Basf Se Verfahren zur isolierung von proteinen aus produktionszellen
CN103282509A (zh) * 2010-12-28 2013-09-04 中外制药株式会社 动物细胞的培养方法
CN104861038B (zh) * 2015-05-08 2019-10-01 永联生物科技(上海)有限公司 一种均质阀组
BR102018004973B1 (pt) * 2018-03-13 2021-10-13 Petróleo Brasileiro S.A. - Petrobras Dispositivo e método para rompimento de células de microorganismos por extrusão
EP4296352A1 (en) 2022-06-20 2023-12-27 LenioBio GmbH Tunable disruption of eukaryotic protoplast to release intact cellular organelles

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR346862A (fr) * 1904-10-07 1905-02-13 Henri Charles Empis Extraction et préparation des substances contenues dans les cellules organiques
GB877898A (en) * 1956-12-06 1961-09-20 Apv Co Ltd A new or improved method of liberating enzymes
US3309032A (en) * 1964-03-23 1967-03-14 Sorvall Inc Ivan Cell fractionator apparatus
DE3810462A1 (de) * 1988-03-26 1989-10-05 Dechema Verfahren zum aufschluss von zellen in einer zellsuspension und vorrichtung zur durchfuehrung des verfahrens

Also Published As

Publication number Publication date
TW426733B (en) 2001-03-21
AU7204496A (en) 1997-04-28
WO1997012959A1 (en) 1997-04-10
EP0854913A1 (en) 1998-07-29
BR9610773A (pt) 1999-07-13
CZ104198A3 (cs) 1998-08-12
SK43498A3 (en) 1998-12-02
CN1203626A (zh) 1998-12-30
EA199800266A1 (ru) 1998-10-29
CN1100868C (zh) 2003-02-05
EA000703B1 (ru) 2000-02-28

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