EA199800266A1 - Способ дезинтеграции культивированных клеток с использованием устройства на основе применения сталкивающихся струй - Google Patents

Способ дезинтеграции культивированных клеток с использованием устройства на основе применения сталкивающихся струй

Info

Publication number
EA199800266A1
EA199800266A1 EA199800266A EA199800266A EA199800266A1 EA 199800266 A1 EA199800266 A1 EA 199800266A1 EA 199800266 A EA199800266 A EA 199800266A EA 199800266 A EA199800266 A EA 199800266A EA 199800266 A1 EA199800266 A1 EA 199800266A1
Authority
EA
Eurasian Patent Office
Prior art keywords
disintegration
application
collaring
jet
device based
Prior art date
Application number
EA199800266A
Other languages
English (en)
Other versions
EA000703B1 (ru
Inventor
Дуглас Б. Сиферт
Франк С. Леу
Original Assignee
Мерк Энд Ко., Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9603759.3A external-priority patent/GB9603759D0/en
Application filed by Мерк Энд Ко., Инк. filed Critical Мерк Энд Ко., Инк.
Publication of EA199800266A1 publication Critical patent/EA199800266A1/ru
Publication of EA000703B1 publication Critical patent/EA000703B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16711—Varicellovirus, e.g. human herpesvirus 3, Varicella Zoster, pseudorabies
    • C12N2710/16751—Methods of production or purification of viral material

Abstract

Описывается новый способ дезинтеграции клеток, у которых отсутствует клеточная стенка, заключающийся в том, что суспендированные клетки пропускают через устройство со сталкивающимися струями при низком давлении. Этот способ обеспечивает дезинтеграцию клеток без повреждения клеточных продуктов, высвобождающихся в процессе разрушения клеток.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA199800266A 1995-10-06 1996-10-02 Способ дезинтеграции культивированных клеток с использованием устройства на основе применения сталкивающихся струй EA000703B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US506295P 1995-10-06 1995-10-06
GBGB9603759.3A GB9603759D0 (en) 1996-02-22 1996-02-22 Method of disrupting cultured cells using an impinging jet device
PCT/US1996/015801 WO1997012959A1 (en) 1995-10-06 1996-10-02 Method of disrupting cultured cells using an impinging jet device

Publications (2)

Publication Number Publication Date
EA199800266A1 true EA199800266A1 (ru) 1998-10-29
EA000703B1 EA000703B1 (ru) 2000-02-28

Family

ID=26308786

Family Applications (1)

Application Number Title Priority Date Filing Date
EA199800266A EA000703B1 (ru) 1995-10-06 1996-10-02 Способ дезинтеграции культивированных клеток с использованием устройства на основе применения сталкивающихся струй

Country Status (10)

Country Link
EP (1) EP0854913A1 (ru)
CN (1) CN1100868C (ru)
AR (1) AR003776A1 (ru)
AU (1) AU7204496A (ru)
BR (1) BR9610773A (ru)
CZ (1) CZ104198A3 (ru)
EA (1) EA000703B1 (ru)
SK (1) SK43498A3 (ru)
TW (1) TW426733B (ru)
WO (1) WO1997012959A1 (ru)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19749735C1 (de) * 1997-11-11 2000-02-10 Invent Gmbh Entwicklung Neuer Technologien Verfahren und Vorrichtung zur Herstellung von Enzymen
NZ533586A (en) 2001-12-20 2005-02-25 Bavarian Nordic As Method for the recovery and purification of poxviruses from infected cells using high pressure homogenisation
DE102005034629B4 (de) * 2005-07-19 2007-09-13 Dr. Hielscher Gmbh Vorrichtung und Verfahren zum mechanischen Aufschluss von Zellen
US20090233348A1 (en) * 2006-08-15 2009-09-17 Basf Se Method for isolating proteins from production cells
RU2615448C2 (ru) * 2010-12-28 2017-04-04 Чугаи Сейяку Кабусики Кайся Описание способа культивирования животной клетки
CN104861038B (zh) * 2015-05-08 2019-10-01 永联生物科技(上海)有限公司 一种均质阀组
BR102018004973B1 (pt) * 2018-03-13 2021-10-13 Petróleo Brasileiro S.A. - Petrobras Dispositivo e método para rompimento de células de microorganismos por extrusão
EP4296352A1 (en) 2022-06-20 2023-12-27 LenioBio GmbH Tunable disruption of eukaryotic protoplast to release intact cellular organelles

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR346862A (fr) * 1904-10-07 1905-02-13 Henri Charles Empis Extraction et préparation des substances contenues dans les cellules organiques
GB877898A (en) * 1956-12-06 1961-09-20 Apv Co Ltd A new or improved method of liberating enzymes
US3309032A (en) * 1964-03-23 1967-03-14 Sorvall Inc Ivan Cell fractionator apparatus
DE3810462A1 (de) * 1988-03-26 1989-10-05 Dechema Verfahren zum aufschluss von zellen in einer zellsuspension und vorrichtung zur durchfuehrung des verfahrens

Also Published As

Publication number Publication date
SK43498A3 (en) 1998-12-02
CN1100868C (zh) 2003-02-05
BR9610773A (pt) 1999-07-13
AR003776A1 (es) 1998-09-09
WO1997012959A1 (en) 1997-04-10
AU7204496A (en) 1997-04-28
CZ104198A3 (cs) 1998-08-12
CN1203626A (zh) 1998-12-30
EP0854913A1 (en) 1998-07-29
EA000703B1 (ru) 2000-02-28
TW426733B (en) 2001-03-21

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Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU