AP2012006530A0 - Methods for measuring enzyme activity useful in determining cell viability in non-purified samples - Google Patents

Methods for measuring enzyme activity useful in determining cell viability in non-purified samples

Info

Publication number
AP2012006530A0
AP2012006530A0 AP2012006530A AP2012006530A AP2012006530A0 AP 2012006530 A0 AP2012006530 A0 AP 2012006530A0 AP 2012006530 A AP2012006530 A AP 2012006530A AP 2012006530 A AP2012006530 A AP 2012006530A AP 2012006530 A0 AP2012006530 A0 AP 2012006530A0
Authority
AP
ARIPO
Prior art keywords
methods
enzyme activity
cell viability
determining cell
purified samples
Prior art date
Application number
AP2012006530A
Other languages
English (en)
Other versions
AP3635A (fr
Inventor
Shawn Mark O'hara
Daniel R Zweitzig
Original Assignee
Zeus Scientific Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44799329&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AP2012006530(A0) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Zeus Scientific Inc filed Critical Zeus Scientific Inc
Publication of AP2012006530A0 publication Critical patent/AP2012006530A0/xx
Application granted granted Critical
Publication of AP3635A publication Critical patent/AP3635A/xx

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AP2012006530A 2010-04-16 2011-04-15 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples AP2012006530A0 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US32493910P 2010-04-16 2010-04-16
US32494910P 2010-04-16 2010-04-16
US32541310P 2010-04-19 2010-04-19
PCT/US2011/032600 WO2011130584A2 (fr) 2010-04-16 2011-04-15 Procédés de mesure de l'activité enzymatique utiles pour la détermination de la viabilité cellulaire dans des échantillons non purifiés

Publications (2)

Publication Number Publication Date
AP2012006530A0 true AP2012006530A0 (en) 2012-10-31
AP3635A AP3635A (fr) 2016-03-09

Family

ID=44799329

Family Applications (1)

Application Number Title Priority Date Filing Date
AP2012006530A AP2012006530A0 (en) 2010-04-16 2011-04-15 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples

Country Status (16)

Country Link
US (3) US20130196318A1 (fr)
EP (3) EP3284831B1 (fr)
JP (2) JP2013537399A (fr)
KR (2) KR20130094714A (fr)
CN (1) CN103328652B (fr)
AP (1) AP2012006530A0 (fr)
AU (2) AU2011239587B2 (fr)
CA (1) CA2796676C (fr)
EA (1) EA201291001A1 (fr)
ES (3) ES2644258T5 (fr)
MY (1) MY161567A (fr)
NZ (2) NZ720529A (fr)
PH (1) PH12016500300A1 (fr)
SG (2) SG184563A1 (fr)
WO (1) WO2011130584A2 (fr)
ZA (1) ZA201208522B (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3284831B1 (fr) 2010-04-16 2019-07-24 Momentum Bioscience Limited Procédés de mesure de l'activité enzymatique utiles pour la détermination de la viabilité cellulaire dans des échantillons non purifiés
JP6388540B2 (ja) * 2012-01-05 2018-09-12 モメンタム・バイオサイエンス・リミテッドMomentum Bioscience Limited 改良したdnaポリメラーゼ活性アッセイおよび生菌の検出を可能にする方法
EP2836610A4 (fr) * 2012-04-12 2015-12-30 Zeus Scientific Inc Procédés de mesure de l'activité de la polymérase pouvant être utilisés en vue de mesures quantitatives sensibles d'une quelconque activité d'extension par la polymérase et pour déterminer la présence de cellules viables
GB2528647A (en) * 2014-07-10 2016-02-03 Momentum Bioscience Ltd Detecting viable microorganisms
GB201412316D0 (en) 2014-07-10 2014-08-27 Momentum Bioscience Ltd Detecting the absence of micro-organisms
US9879328B2 (en) * 2014-11-21 2018-01-30 GeneWeave Biosciences, Inc. Mechanisms of antimicrobial susceptibility
US10662484B1 (en) 2016-03-24 2020-05-26 Bench in a Box, LLC Two universal duplex primer sets and a combined real-time PCR and high resolution melt analysis assay for the amplification of gram-positive and gram-negative bacteria
GB201805479D0 (en) * 2018-04-03 2018-05-16 Momentum Bioscience Ltd Microorganism separation and detection
GB201914538D0 (en) 2019-10-08 2019-11-20 Momentum Bioscience Ltd Microorganism capture from antimicrobial-containing solution
US20230112608A1 (en) 2021-10-13 2023-04-13 Disruptive Oil And Gas Technologies Corp Nanobubble dispersions generated in electrochemically activated solutions
CN114032285B (zh) * 2021-11-24 2022-05-24 北京擎科生物科技有限公司 PolyA聚合酶活性检测方法

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS501953B1 (fr) 1968-08-05 1975-01-22
JPS505942B1 (fr) 1970-08-23 1975-03-08
JPS6054697A (ja) * 1983-09-05 1985-03-29 イザド−ラ ブロドスキイ 診断方法および装置
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
SE462497B (sv) 1988-11-30 1990-07-02 Clas Fredrik Runesson Kaelland Saett foer att aktivitetsbestaemma polymeraser
DE69028725T2 (de) * 1989-02-28 1997-03-13 Canon Kk Partiell doppelsträngiges Oligonukleotid und Verfahren zu seiner Bildung
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
DE4027616A1 (de) * 1990-08-31 1992-03-05 Boehringer Mannheim Gmbh Verfahren zur bestimmung von polymerase-aktivitaet
JPH06509477A (ja) 1992-05-11 1994-10-27 シュープバッハ・イエルク 逆転写酵素の検出方法
US5629154A (en) 1993-11-12 1997-05-13 Geron Corporation Telomerase activity assays
US5489508A (en) 1992-05-13 1996-02-06 University Of Texas System Board Of Regents Therapy and diagnosis of conditions related to telomere length and/or telomerase activity
JPH0622798A (ja) * 1992-07-07 1994-02-01 Hitachi Ltd 塩基配列決定法
US5719028A (en) * 1992-12-07 1998-02-17 Third Wave Technologies Inc. Cleavase fragment length polymorphism
US5863726A (en) 1993-11-12 1999-01-26 Geron Corporation Telomerase activity assays
HU220855B1 (en) 1994-07-13 2002-06-29 Secr Defence Brit Process and method of investigation of the presence and/or amount of microorganisms and/or intracellular materials thereof
US6054278A (en) * 1997-05-05 2000-04-25 The Perkin-Elmer Corporation Ribosomal RNA gene polymorphism based microorganism identification
DE60042453D1 (de) * 1999-07-28 2009-08-06 Kaken Pharma Co Ltd Verfahren zum nachweis pathogener mikroorganismen und antimikrobieller mittel, verfahren zum nachweis der arzneimittelwirkung antimikrobieller mittel und antimikrobielle mittel
GB9923846D0 (en) 1999-10-09 1999-12-08 Quip Technology Limited Reverse transcriptase assay
AU2003260815A1 (en) * 2002-08-08 2004-02-25 University Of Lausanne Method for the rapid assessment of the presence and viability of bacterial cells and use thereof________________________
US20050136439A1 (en) * 2003-09-12 2005-06-23 North Carolina State University Novel methods of inorganic compound discovery and synthesis
GB0508981D0 (en) * 2005-05-03 2005-06-08 Acolyte Biomedica Ltd Distinguishing cells in a sample
AU2006248724B2 (en) * 2005-05-18 2011-07-21 Iseao Technologies Limited Improved methods and kits for detecting an enzyme capable of modifying a nucleic acid
US20080318215A1 (en) * 2005-12-20 2008-12-25 Ming-Sheng Lee Apparatus, methods and products for detecting genetic mutation
US7947447B2 (en) * 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
JP2010532986A (ja) 2007-07-09 2010-10-21 マイクロセンス メドテック リミテッド 改良された微生物の検出
EP3284831B1 (fr) 2010-04-16 2019-07-24 Momentum Bioscience Limited Procédés de mesure de l'activité enzymatique utiles pour la détermination de la viabilité cellulaire dans des échantillons non purifiés

Also Published As

Publication number Publication date
CN103328652A (zh) 2013-09-25
SG184563A1 (en) 2012-11-29
JP2017148067A (ja) 2017-08-31
EA201291001A1 (ru) 2013-09-30
US10870894B2 (en) 2020-12-22
US10876173B2 (en) 2020-12-29
EP3284831A1 (fr) 2018-02-21
CN103328652B (zh) 2016-12-28
EP2558600B1 (fr) 2017-10-04
KR20180086526A (ko) 2018-07-31
PH12016500300A1 (en) 2019-02-04
EP2558600A2 (fr) 2013-02-20
CA2796676C (fr) 2020-07-14
AP3635A (fr) 2016-03-09
NZ701866A (en) 2016-07-29
MY161567A (en) 2017-04-28
ES2906061T3 (es) 2022-04-13
EP2558600B2 (fr) 2020-06-17
JP2013537399A (ja) 2013-10-03
US20170321287A1 (en) 2017-11-09
EP2558600A4 (fr) 2014-04-30
ES2644258T3 (es) 2017-11-28
ES2644258T5 (es) 2021-02-02
JP6603956B2 (ja) 2019-11-13
KR20130094714A (ko) 2013-08-26
ES2739806T3 (es) 2020-02-04
WO2011130584A2 (fr) 2011-10-20
ZA201208522B (en) 2014-08-27
US20180230551A1 (en) 2018-08-16
AU2011239587A1 (en) 2012-11-08
AU2016203878A1 (en) 2016-06-30
WO2011130584A3 (fr) 2013-07-11
SG10201502900PA (en) 2015-06-29
EP3617320A1 (fr) 2020-03-04
AU2011239587B2 (en) 2016-03-10
EP3284831B1 (fr) 2019-07-24
EP3617320B1 (fr) 2022-01-26
US20130196318A1 (en) 2013-08-01
NZ720529A (en) 2017-01-27
CA2796676A1 (fr) 2011-10-20
KR102068136B1 (ko) 2020-01-20

Similar Documents

Publication Publication Date Title
SG10201502900PA (en) Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
HK1198262A1 (en) Measuring volume and constituents of cells
SG10201601174XA (en) Automated System For Sample Preparation And Analysis
HK1206813A1 (en) Method for determining analyte concentration in a sample
HK1243375A1 (zh) 樣本分析系統
HK1191015A1 (en) Novel ferrocene labels for electrochemical assay and their use in analytical methods
HK1169454A1 (en) Method for single cell genome analysis and kit therefor
EP2595526A4 (fr) Système et procédé de mesure d'un analyte dans un échantillon
EP2596006A4 (fr) Procédés et systèmes d'analyse de cellules isolées
HK1192945A1 (en) Capacitance detection in electrochemical assay with improved sampling time offset
EP2531860A4 (fr) Mesure d'échantillons à multiples analytes à l'aide d'une cuve à circulation en ligne
EP2638380A4 (fr) Procédé d'échantillonnage destiné à être utilisé dans une mesure d'odeur
EP2707724A4 (fr) Concentration d'enzymes et dosages
EP2446048A4 (fr) Méthodes et kits de mesure de l 'activité enzymatique
EP2708880A4 (fr) Dispositif de mesure d'un échantillon biologique et procédé de mesure d'un échantillon biologique l'utilisant
EP2553115A4 (fr) Procédés de culture et d'analyse de cellules
EP2789692A4 (fr) Méthode de mesure de cellules et réactif pour la mesure des cellules
EP2576809A4 (fr) Mesure de viabilité et de concentration de levures
EP2700707A4 (fr) Procédé de culture de cellules et kit de culture de cellules
BR112012021878A2 (pt) método para detectar um deriva de clorofila livre de fitol em uma amostra, método de análise para determinar a quantidade de atividade de enzina em uma amostra e kit para determinar a quantidade de atividade de enzima em uma amostra
GB201113641D0 (en) Cell culture support and cell culture method
EP2700950A4 (fr) Procédé pour l'analyse d'interaction protéine-protéine au niveau d'une molécule unique dans un environnement cellulaire, et procédé pour la mesure de la densité de protéine activée dans du cytosol
EP2839282A4 (fr) Support solide et procédé pour la détection d'un analyte dans un échantillon
GB201010233D0 (en) Improvements in or relating to sample analysis
BR112013014568A2 (pt) ensaio de viabilidade celular 3d