ZA985758B - Assaying nucleotides in solution using a fluorescent intensity quenching effect - Google Patents

Assaying nucleotides in solution using a fluorescent intensity quenching effect

Info

Publication number
ZA985758B
ZA985758B ZA985758A ZA985758A ZA985758B ZA 985758 B ZA985758 B ZA 985758B ZA 985758 A ZA985758 A ZA 985758A ZA 985758 A ZA985758 A ZA 985758A ZA 985758 B ZA985758 B ZA 985758B
Authority
ZA
South Africa
Prior art keywords
solution
fluorescent intensity
quenching effect
intensity quenching
assaying
Prior art date
Application number
ZA985758A
Other languages
English (en)
Inventor
Yuan Min Wu
Eileen Xiao-Feng Nie
Original Assignee
Lorne Park Res Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lorne Park Res Inc filed Critical Lorne Park Res Inc
Publication of ZA985758B publication Critical patent/ZA985758B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ZA985758A 1997-07-01 1998-07-01 Assaying nucleotides in solution using a fluorescent intensity quenching effect ZA985758B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/886,280 US5846729A (en) 1997-02-27 1997-07-01 Assaying nucleotides in solution using a fluorescent intensity quenching effect

Publications (1)

Publication Number Publication Date
ZA985758B true ZA985758B (en) 1999-01-27

Family

ID=25388774

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA985758A ZA985758B (en) 1997-07-01 1998-07-01 Assaying nucleotides in solution using a fluorescent intensity quenching effect

Country Status (10)

Country Link
US (1) US5846729A (xx)
EP (1) EP1002125A1 (xx)
JP (1) JP2001510681A (xx)
AR (1) AR016125A1 (xx)
AU (1) AU730865B2 (xx)
CA (1) CA2295333C (xx)
IN (1) IN189178B (xx)
TW (1) TWI235765B (xx)
WO (1) WO1999001578A1 (xx)
ZA (1) ZA985758B (xx)

Families Citing this family (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6555692B1 (en) * 1996-02-26 2003-04-29 California Institute Of Technology Preparation and use of bifunctional molecules having DNA sequence binding specificity
SE9601318D0 (sv) * 1996-04-04 1996-04-04 Pharmacia Biosensor Ab Method for nucleic acid analysis
US6251591B1 (en) * 1997-02-27 2001-06-26 Lorne Park Research, Inc. Quantitative method for detecting nucleotide concentration
US6046004A (en) * 1997-02-27 2000-04-04 Lorne Park Research, Inc. Solution hybridization of nucleic acids with antisense probes having modified backbones
DE69841849D1 (de) 1997-10-27 2010-09-30 Boston Probes Inc Sich auf "pna molecular beacons" beziehende verfahren, testsätze und zusammensetzungen
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
WO1999049293A2 (en) * 1998-03-24 1999-09-30 Boston Probes, Inc. Methods, kits and compositions pertaining to detection complexes
US6420115B1 (en) 1999-12-21 2002-07-16 Ingeneus Corporation Cation mediated triplex hybridization assay
US6403313B1 (en) * 1999-12-21 2002-06-11 Ingeneus Corporation Fluorescent intensity assay for duplex and triplex nucleic acid hybridization solution utilizing fluorescent intercalators
US6294333B1 (en) * 1998-12-31 2001-09-25 Ingeneus Corp. Fluorescent intensity assay for protein or peptide binding to nucleic acids
US6656692B2 (en) * 1999-12-21 2003-12-02 Ingeneus Corporation Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US6858390B2 (en) 1998-12-31 2005-02-22 Ingeneus Corporation Aptamers containing sequences of nucleic acid or nucleic acid analogues bound homologously, or in novel complexes
PT1471926E (pt) 1999-06-01 2011-03-11 Baylor College Medicine Composições e métodos para a utilização terapêutica de uma sequência associada a atonal
JP2003510017A (ja) 1999-06-22 2003-03-18 インビトロジェン コーポレイション 核酸の検出および識別のために改良されたプライマーおよび方法
US6645733B1 (en) 1999-06-25 2003-11-11 Ingeneus Corporation Fluorescent intensity method for assaying binding between proteins or peptides
US6830902B1 (en) 1999-07-02 2004-12-14 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
WO2001038585A2 (en) * 1999-11-24 2001-05-31 The Regents Of The University Of California Polymer arrays and methods of using labeled probe molecules to identify and quantify target molecule expression
US6927027B2 (en) 1999-12-21 2005-08-09 Ingeneus Corporation Nucleic acid multiplex formation
US6924108B2 (en) * 1999-12-21 2005-08-02 Ingeneus Corporation Nucleic acid binding enhancement by conjugation with nucleotides, nucleosides, bases and/or their analogues
US7309569B2 (en) * 1999-12-21 2007-12-18 Ingeneus, Inc. Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US20030181412A1 (en) * 1999-12-21 2003-09-25 Ingeneus Corporation Method for modifying transcription and/or translation in an organism for therapeutic, prophylactic and/or analytic uses
US6911536B1 (en) 1999-12-21 2005-06-28 Ingeneus Corporation Triplex and quadruplex catalytic hybridization
US7052844B2 (en) * 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
US6982147B2 (en) * 2000-01-24 2006-01-03 Ingeneus Corporation Apparatus for assaying biopolymer binding by means of multiple measurements under varied conditions
US7220541B2 (en) 2000-01-24 2007-05-22 Ingeneus, Inc. Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions
US20030170659A1 (en) * 2000-01-24 2003-09-11 Ingeneus Corporation Electrical treatment of binding media to encourage, discourage and/or study biopolymer binding
US6265170B1 (en) 2000-01-24 2001-07-24 Ingeneus Corporation Homogenous assay of duplex of triplex hybridization by means of multiple measurements under varied conditions
US6613524B1 (en) 2000-01-24 2003-09-02 Ingeneus Corporation Amperometric affinity assay and electrically stimulated complexes of nucleic acids
US20020155427A1 (en) * 2000-04-03 2002-10-24 Cohenford Menashi A. Detection and typing of human papillomavirus using PNA probes
US6936443B2 (en) * 2000-04-03 2005-08-30 Cytyc Corporation Detection and typing of human papillomavirus using PNA probes
US20060073530A1 (en) * 2001-08-15 2006-04-06 Olaf Schneewind Methods and compositions involving sortase B
US20030165859A1 (en) 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
DE10155031A1 (de) * 2001-11-09 2003-05-28 Friz Biochem Gmbh Fluoreszenz-Quenchen zur Detektion von Ligat-Ligand Assoziationsereignissen in elektrischen Feldern
EP1572902B1 (en) 2002-02-01 2014-06-11 Life Technologies Corporation HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES
CA2474910A1 (en) 2002-02-01 2003-08-07 Sequitur, Inc. Oligonucleotide compositions with enhanced efficiency
US20060009409A1 (en) 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
US6818420B2 (en) 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
AU2003258168B2 (en) 2002-08-12 2009-10-29 Sillajen Biotherapeutics, Inc. Methods and compositions concerning poxviruses and cancer
US20040180345A1 (en) * 2003-03-14 2004-09-16 Ingeneus Corporation Pre-incubation method to improve signal/noise ratio of nucleic acid assays
WO2006031955A2 (en) 2004-09-14 2006-03-23 The Regents Of The University Of Colorado, A Body Corporate Method for treatment with bucindolol based on genetic targeting
WO2006033088A2 (en) * 2004-09-24 2006-03-30 Ingeneus Inc. Genomic assay
JP2006254784A (ja) * 2005-03-17 2006-09-28 Institute Of Physical & Chemical Research プライマーダイマーからの非特異的増幅を減少させる方法
WO2007030668A2 (en) 2005-09-07 2007-03-15 Jennerex Biotherapeutics Ulc Systemic treatment of metastatic and/or systemically-disseminated cancers using gm-csf-expressing poxviruses
US8980246B2 (en) 2005-09-07 2015-03-17 Sillajen Biotherapeutics, Inc. Oncolytic vaccinia virus cancer therapy
EP1785490B1 (en) * 2005-10-17 2007-11-14 RiNA Netzwerk RNA-Technologien GmbH Method for determining an unknown PNA sequence and uses thereof
ES2551892T3 (es) 2006-09-15 2015-11-24 Ottawa Health Research Institute Rhabdovirus oncolítico
WO2010111656A2 (en) * 2009-03-27 2010-09-30 Life Technologies Corporation Systems and methods for assessing images
JP5785942B2 (ja) 2009-06-29 2015-09-30 ルミネックス コーポレーション ヘアピンコンフォメーションを有するキメラプライマーおよびその使用法
US9409983B2 (en) 2009-07-23 2016-08-09 The Board Of Trustess Of The University Of Illinois Methods and compositions involving PBEF inhibitors for lung inflammation conditions and diseases
WO2011032088A1 (en) 2009-09-11 2011-03-17 Arca Biopharma, Inc. Polymorphisms in the pde3a gene
JP5879266B2 (ja) 2009-09-14 2016-03-08 シラジェン バイオセラピューティクス インコーポレイテッド 腫瘍崩壊性ワクシニアウイルスの併用癌療法
CA2836117C (en) 2009-12-10 2017-08-15 Ottawa Hospital Research Institute Oncolytic rhabdovirus
EP2515899B1 (en) 2009-12-23 2016-05-25 ARCA biopharma, Inc. Methods and compositions for cardiovascular diseases and conditions
JP6121910B2 (ja) 2011-01-04 2017-04-26 シラジェン バイオセラピューティクス インコーポレイテッド 腫瘍溶解性ワクシニアウイルスの投与による腫瘍抗原に対する抗体の生成および腫瘍特異的補体依存性細胞傷害の生成
WO2012158238A2 (en) 2011-02-28 2012-11-22 University Of Iowa Research Foundation Anti-müllerian hormone changes in pregnancy and prediction ofadverse pregnancy outcomes and gender
WO2012120377A2 (en) 2011-03-08 2012-09-13 King Abdullah University Of Science And Technology Molecular biomarker set for early detection of ovarian cancer
US9364532B2 (en) 2011-06-08 2016-06-14 Children's Hospital Of Eastern Ontario Research Institute Inc. Compositions and methods for glioblastoma treatment
RU2483049C2 (ru) * 2011-07-21 2013-05-27 Открытое акционерное общество "Научно-исследовательский институт полимерных материалов" Способ изготовления партии многошашечных зарядов твердого ракетного топлива
WO2013036799A2 (en) 2011-09-09 2013-03-14 Fred Hutchinson Cancer Research Center Methods and compositions involving nkg2d inhibitors and cancer
WO2014116721A1 (en) 2013-01-22 2014-07-31 The Arizona Board Of Regents For And On Behalf Of Arizona State University Geminiviral vector for expression of rituximab
EP2958994B1 (en) 2013-02-21 2019-05-29 Turnstone Limited Partnership Vaccine composition
AU2014329535B2 (en) 2013-10-03 2017-09-07 Oklahoma Medical Research Foundation Biomarkers for systemic lupus erythematosus disease activity, and intensity and flare
EP3224362A4 (en) 2014-11-26 2018-06-06 The Regents of The University of California Therapeutic compositions comprising transcription factors and methods of making and using the same
AU2016324165B2 (en) 2015-09-16 2020-07-02 PetaOmics, Inc. Methods and compositions for genomic target enrichment and selective DNA sequencing
AU2018213315A1 (en) 2017-01-26 2019-07-25 Oklahoma Medical Research Foundation Biomarkers for systemic lupus erythematosus disease activity, and intensity and flare
AU2019360758B2 (en) 2018-10-18 2024-08-08 Oklahoma Medical Research Foundation Biomarkers for a Systemic Lupus Erythematosus (SLE) disease activity immune index that characterizes disease activity
CN113614535B (zh) 2018-10-31 2024-08-16 亚利桑那大学评议会 用于辐射诱导的肺损伤的生物标志物和使用方法

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4220450A (en) * 1978-04-05 1980-09-02 Syva Company Chemically induced fluorescence immunoassay
FR2558172B1 (fr) * 1984-01-16 1986-06-13 Inst Nat Sante Rech Med Sonde contenant un acide nucleique modifie et reconnaissance par des anticorps specifiques et utilisation de cette sonde et de ces anticorps specifiques pour detecter et caracteriser une sequence d'adn homologue
US5217866A (en) * 1985-03-15 1993-06-08 Anti-Gene Development Group Polynucleotide assay reagent and method
DE3785591T2 (de) * 1986-01-10 1993-09-02 Amoco Corp Kompetitiver homogener test.
WO1992018650A1 (en) * 1991-04-11 1992-10-29 Baxter Diagnostics Inc. Detection of dna/rna by fluorescence polarization
WO1992018560A1 (fr) * 1991-04-22 1992-10-29 Takata Corporation Element comportant un enduit en surface
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5539082A (en) * 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5332659A (en) * 1992-04-09 1994-07-26 The United States Of America As Represented By The Secretary Of The Navy Light emission-or absorbance-based binding assays for polynucleic acids
DE4216949C2 (de) * 1992-05-22 1997-07-24 Christoph Prof Dr Dr Cremer Nichtenzymatisches Verfahren zur In-situ-Hybridisierung bei spezifischen Proben
US5674698A (en) * 1992-09-14 1997-10-07 Sri International Up-converting reporters for biological and other assays using laser excitation techniques
US5503980A (en) * 1992-11-06 1996-04-02 Trustees Of Boston University Positional sequencing by hybridization
US5445935A (en) * 1992-11-23 1995-08-29 Royer; Catherine A. Quantitative detection of macromolecules with fluorescent oligonucleotides
DE69333991T2 (de) * 1992-11-27 2006-12-14 Canon K.K. Verfahren und Sonde zum Nachweis von Nukleinsäuren
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5723288A (en) * 1994-05-06 1998-03-03 The University Of North Carolina At Chapel Hill Method of fluorescent detection of nucleic acids and cytoskeleton elements using bis-dicationic aryl furans, and kits useful therefor
US5747247A (en) * 1994-07-25 1998-05-05 The Regents Of The University Of California Spectroscopic helicase assay
EP0826066B1 (en) * 1995-05-05 2000-09-13 The Perkin-Elmer Corporation Methods and reagents for combined pcr amplification and hybridization probing assay
JPH11512617A (ja) * 1995-10-06 1999-11-02 パーセプテイブ・バイオシステムズ・インコーポレイテツド ペプチド核酸プローブを用いるハイブリダイゼーション分析のための方法、装置及びキット
CA2190430A1 (en) * 1995-12-12 1997-06-13 Margret Barbara Basinski Method for measuring genetic messages

Also Published As

Publication number Publication date
CA2295333A1 (en) 1999-01-14
AR016125A1 (es) 2001-06-20
WO1999001578A1 (en) 1999-01-14
AU730865B2 (en) 2001-03-15
IN189178B (xx) 2003-01-04
US5846729A (en) 1998-12-08
TWI235765B (en) 2005-07-11
JP2001510681A (ja) 2001-08-07
CA2295333C (en) 2005-03-01
AU8030298A (en) 1999-01-25
EP1002125A1 (en) 2000-05-24

Similar Documents

Publication Publication Date Title
ZA985758B (en) Assaying nucleotides in solution using a fluorescent intensity quenching effect
ZA981660B (en) Assaying nucleotides in solution using PNA probes
ZA98485B (en) Energy guiding chain
HUP0100194A3 (en) Fluorescent lamp
GB9718081D0 (en) Fluorescent whitening agent
GB9722032D0 (en) Identifying substances
EP1093150A4 (en) photomultiplier
GB2323163B (en) Flicker photometer
ZA989432B (en) Cancer cell nuclease activator
KR200160828Y1 (en) Backlight structure
GB9707743D0 (en) Analysis
HK1026221A1 (en) Novel disazo dyestuffs
TW582620U (en) Fluorescent light and fluorescent light device
GB2323162B (en) Flicker photometer
GB9808230D0 (en) Improved exit sign
TW358451U (en) Three in one chain structure
GB9717898D0 (en) Signs
ZA987267B (en) Nucleosides
GB9814715D0 (en) Fluorescence analysis
CA81022S (en) Fluorescent lamp
GB2356701B (en) Flicker photometer
AU1605P (en) BFP-788 Bright Scarlet Pelargonium xhortorum
GB9820064D0 (en) Energy saving displays
GB9700610D0 (en) Constructional kits
GR2001735Y (en) Energy saver for fluorescent lamps