ZA966315B - Method for the purification of proteins - Google Patents

Method for the purification of proteins

Info

Publication number
ZA966315B
ZA966315B ZA966315A ZA966315A ZA966315B ZA 966315 B ZA966315 B ZA 966315B ZA 966315 A ZA966315 A ZA 966315A ZA 966315 A ZA966315 A ZA 966315A ZA 966315 B ZA966315 B ZA 966315B
Authority
ZA
South Africa
Prior art keywords
pct
proteins
purification
sec
deionized water
Prior art date
Application number
ZA966315A
Other languages
English (en)
Inventor
Catherine Georges
Pierre Francois Fauquex
Original Assignee
Ciba Geigy Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciba Geigy Ag filed Critical Ciba Geigy Ag
Publication of ZA966315B publication Critical patent/ZA966315B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
    • Y10S530/811—Peptides or proteins is immobilized on, or in, an inorganic carrier
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
    • Y10S530/812—Peptides or proteins is immobilized on, or in, an organic carrier

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
ZA966315A 1995-07-26 1996-07-25 Method for the purification of proteins ZA966315B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP95810485 1995-07-26

Publications (1)

Publication Number Publication Date
ZA966315B true ZA966315B (en) 1997-01-27

Family

ID=8221776

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA966315A ZA966315B (en) 1995-07-26 1996-07-25 Method for the purification of proteins

Country Status (13)

Country Link
US (1) US5990289A (pt)
EP (1) EP0840746B1 (pt)
JP (1) JP3833255B2 (pt)
AT (1) ATE278705T1 (pt)
AU (1) AU6700796A (pt)
CA (1) CA2226080A1 (pt)
DE (1) DE69633567T2 (pt)
DK (1) DK0840746T3 (pt)
ES (1) ES2227603T3 (pt)
PT (1) PT840746E (pt)
TW (1) TW442492B (pt)
WO (1) WO1997005159A2 (pt)
ZA (1) ZA966315B (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6479300B1 (en) * 1999-03-15 2002-11-12 Millipore Corporation Metal loaded ligand bound membranes for metal ion affinity chromatography
US7128904B2 (en) * 2001-01-16 2006-10-31 The Regents Of The University Of Michigan Material containing metal ion ligand complex producing nitric oxide in contact with blood
US7335383B2 (en) * 2001-01-16 2008-02-26 The Regents Of The University Of Michigan Generation of nitric oxide in vivo from nitrite, nitrate or nitrosothiols endogenous in blood
CA2447594A1 (en) * 2001-06-21 2003-01-03 Clontech Laboratories, Inc. Water-soluble polymeric metal ion affinity compositions and methods for using the same
PL368755A1 (en) * 2001-11-28 2005-04-04 Sandoz Ag Chromatographic purification of recombinant human erythropoietin
US7294614B2 (en) * 2004-10-12 2007-11-13 Clontech Laboratories, Inc. Phosphoprotein affinity resins and methods for making and using the same
US20080241208A1 (en) * 2005-06-30 2008-10-02 Charles Shanley Methods, Compositions and Devices For Promoting Anglogenesis
ATE485849T1 (de) * 2005-12-02 2010-11-15 Univ Michigan Polymerzusammensetzungen, überzüge und vorrichtungen und herstellungs- und anwendungsverfahren damit
CA2821711C (en) 2010-12-15 2017-10-10 Baxter International Inc. Eluate collection using conductivity gradient

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES8302778A1 (es) * 1980-11-10 1983-02-01 Genentech Inc Un procedimiento para producir un polipeptido antiviral.
DE3685996T2 (de) * 1985-06-11 1993-01-14 Ciba Geigy Ag Hybrid-interferone.
CA1304886C (en) * 1986-07-10 1992-07-07 Heinz Dobeli Metal chelate resins
US5169936A (en) * 1989-04-14 1992-12-08 Biogen, Inc. Protein purification on immobilized metal affinity resins effected by elution using a weak ligand
US5136025A (en) * 1990-04-04 1992-08-04 California Biotechnology Inc. Method to purify basic fibroblast growth factor

Also Published As

Publication number Publication date
EP0840746A2 (en) 1998-05-13
JPH11509853A (ja) 1999-08-31
DK0840746T3 (da) 2004-11-29
US5990289A (en) 1999-11-23
WO1997005159A3 (en) 1997-03-06
JP3833255B2 (ja) 2006-10-11
CA2226080A1 (en) 1997-02-13
DE69633567T2 (de) 2006-02-23
WO1997005159A2 (en) 1997-02-13
ATE278705T1 (de) 2004-10-15
DE69633567D1 (de) 2004-11-11
AU6700796A (en) 1997-02-26
TW442492B (en) 2001-06-23
ES2227603T3 (es) 2005-04-01
PT840746E (pt) 2004-12-31
EP0840746B1 (en) 2004-10-06

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