ATE278705T1 - Verfahren zur reinigung von proteinen - Google Patents
Verfahren zur reinigung von proteinenInfo
- Publication number
- ATE278705T1 ATE278705T1 AT96927033T AT96927033T ATE278705T1 AT E278705 T1 ATE278705 T1 AT E278705T1 AT 96927033 T AT96927033 T AT 96927033T AT 96927033 T AT96927033 T AT 96927033T AT E278705 T1 ATE278705 T1 AT E278705T1
- Authority
- AT
- Austria
- Prior art keywords
- pct
- sec
- deionized water
- date
- washed
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 239000008367 deionised water Substances 0.000 abstract 2
- 229910021641 deionized water Inorganic materials 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 abstract 1
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 abstract 1
- 239000002879 Lewis base Substances 0.000 abstract 1
- 238000001042 affinity chromatography Methods 0.000 abstract 1
- 229910052802 copper Inorganic materials 0.000 abstract 1
- 239000010949 copper Substances 0.000 abstract 1
- 150000007527 lewis bases Chemical class 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/56—IFN-alpha
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
- Y10S530/811—Peptides or proteins is immobilized on, or in, an inorganic carrier
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
- Y10S530/812—Peptides or proteins is immobilized on, or in, an organic carrier
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP95810485 | 1995-07-26 | ||
| PCT/EP1996/003144 WO1997005159A2 (en) | 1995-07-26 | 1996-07-17 | Method for the purification of proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE278705T1 true ATE278705T1 (de) | 2004-10-15 |
Family
ID=8221776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT96927033T ATE278705T1 (de) | 1995-07-26 | 1996-07-17 | Verfahren zur reinigung von proteinen |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US5990289A (de) |
| EP (1) | EP0840746B1 (de) |
| JP (1) | JP3833255B2 (de) |
| AT (1) | ATE278705T1 (de) |
| AU (1) | AU6700796A (de) |
| CA (1) | CA2226080A1 (de) |
| DE (1) | DE69633567T2 (de) |
| DK (1) | DK0840746T3 (de) |
| ES (1) | ES2227603T3 (de) |
| PT (1) | PT840746E (de) |
| TW (1) | TW442492B (de) |
| WO (1) | WO1997005159A2 (de) |
| ZA (1) | ZA966315B (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6479300B1 (en) * | 1999-03-15 | 2002-11-12 | Millipore Corporation | Metal loaded ligand bound membranes for metal ion affinity chromatography |
| US7128904B2 (en) * | 2001-01-16 | 2006-10-31 | The Regents Of The University Of Michigan | Material containing metal ion ligand complex producing nitric oxide in contact with blood |
| US7335383B2 (en) * | 2001-01-16 | 2008-02-26 | The Regents Of The University Of Michigan | Generation of nitric oxide in vivo from nitrite, nitrate or nitrosothiols endogenous in blood |
| ATE394412T1 (de) * | 2001-06-21 | 2008-05-15 | Clontech Lab Inc | Wasserlösliche polymere zusammensetzungen mit affinität für metallionen und verfahren zu deren anwendung |
| CN1596268A (zh) * | 2001-11-28 | 2005-03-16 | 桑多斯有限公司 | 重组人红细胞生成素的层析纯化 |
| US7294614B2 (en) * | 2004-10-12 | 2007-11-13 | Clontech Laboratories, Inc. | Phosphoprotein affinity resins and methods for making and using the same |
| WO2007005758A2 (en) * | 2005-06-30 | 2007-01-11 | Mc3, Inc. | Methods , compositions and devices for promoting angiogenesis |
| CA2632224A1 (en) * | 2005-12-02 | 2007-06-07 | The Regents Of The University Of Michigan | Polymer compositions, coatings and devices, and methods of making and using the same |
| AU2011343813B2 (en) | 2010-12-15 | 2015-05-21 | Takeda Pharmaceutical Company Limited | Eluate collection using conductivity gradient |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES506955A0 (es) * | 1980-11-10 | 1983-02-01 | Genentech Inc | Un procedimiento para producir un polipeptido antiviral. |
| DE3685996T2 (de) * | 1985-06-11 | 1993-01-14 | Ciba Geigy Ag | Hybrid-interferone. |
| CA1304886C (en) * | 1986-07-10 | 1992-07-07 | Heinz Dobeli | Metal chelate resins |
| US5169936A (en) * | 1989-04-14 | 1992-12-08 | Biogen, Inc. | Protein purification on immobilized metal affinity resins effected by elution using a weak ligand |
| US5136025A (en) * | 1990-04-04 | 1992-08-04 | California Biotechnology Inc. | Method to purify basic fibroblast growth factor |
-
1996
- 1996-07-12 TW TW085108439A patent/TW442492B/zh not_active IP Right Cessation
- 1996-07-17 JP JP50718397A patent/JP3833255B2/ja not_active Expired - Fee Related
- 1996-07-17 CA CA002226080A patent/CA2226080A1/en not_active Abandoned
- 1996-07-17 DE DE69633567T patent/DE69633567T2/de not_active Expired - Fee Related
- 1996-07-17 PT PT96927033T patent/PT840746E/pt unknown
- 1996-07-17 EP EP96927033A patent/EP0840746B1/de not_active Expired - Lifetime
- 1996-07-17 ES ES96927033T patent/ES2227603T3/es not_active Expired - Lifetime
- 1996-07-17 DK DK96927033T patent/DK0840746T3/da active
- 1996-07-17 AU AU67007/96A patent/AU6700796A/en not_active Abandoned
- 1996-07-17 US US09/000,031 patent/US5990289A/en not_active Expired - Fee Related
- 1996-07-17 WO PCT/EP1996/003144 patent/WO1997005159A2/en not_active Ceased
- 1996-07-17 AT AT96927033T patent/ATE278705T1/de not_active IP Right Cessation
- 1996-07-25 ZA ZA966315A patent/ZA966315B/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP0840746B1 (de) | 2004-10-06 |
| WO1997005159A3 (en) | 1997-03-06 |
| JP3833255B2 (ja) | 2006-10-11 |
| JPH11509853A (ja) | 1999-08-31 |
| WO1997005159A2 (en) | 1997-02-13 |
| DE69633567T2 (de) | 2006-02-23 |
| PT840746E (pt) | 2004-12-31 |
| CA2226080A1 (en) | 1997-02-13 |
| DK0840746T3 (da) | 2004-11-29 |
| AU6700796A (en) | 1997-02-26 |
| EP0840746A2 (de) | 1998-05-13 |
| DE69633567D1 (de) | 2004-11-11 |
| ES2227603T3 (es) | 2005-04-01 |
| ZA966315B (en) | 1997-01-27 |
| TW442492B (en) | 2001-06-23 |
| US5990289A (en) | 1999-11-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69907439D1 (de) | Reinigung von proteinen durch ionenaustauschchromatographie | |
| BR9809292A (pt) | Métodos aperfeiçoados para o processamento de proteìna c ativada | |
| MX9603637A (es) | Purificacion de anticuerpo. | |
| ATE278785T1 (de) | Neues verfahren zur gewinnung und reinigung von fusionsproteinen | |
| ZA944950B (en) | Protein purification | |
| FI942625L (fi) | Peptidisiä fosfinyylioksimetyyliketoneja interleukiini-1beta-muuttuvan entsyymin inhibiittoreina | |
| IN181859B (de) | ||
| DK0565168T3 (da) | Fremgangsmåde til fremstilling af oprenset enzym | |
| PT848752E (pt) | Processo de purificacao de virus por cromatografia | |
| DK0789767T3 (da) | Fremgangsmåde til fremstilling af oprensede papilomavirusproteiner | |
| DE69633567D1 (de) | Verfahren zur reinigung von proteinen | |
| EP0656419A4 (de) | Verfahren zur hochreinigung von menschlichem serumalbumin. | |
| DE3767745D1 (de) | Verfahren zur ph-pufferung. | |
| DE69613685D1 (de) | D-aminosäure-oxidase kodierendes dns-fragment | |
| ATE496939T1 (de) | Methode zur reinigung von keratinozyten- wachstumfaktoren | |
| ATE351909T1 (de) | Neue peptidfragmente zur reinigung von proteinen | |
| DE69101425D1 (de) | Methode zur Reinigung von rohem glycidyl (Meth)-Acrylat. | |
| FI946031A7 (fi) | Kringleä sisältävien proteiinien puhdistus ja etenkin t-PA:n | |
| DE59813125D1 (de) | Srcr domäne-enthaltendes protein | |
| KR970009339B1 (en) | Tpa from scolopendra subepinpes multilans and its purification method | |
| AR002059A1 (es) | Procedimiento para aislar y purificar una proteina de plasma humano con actividad fibrinolitica | |
| ATE104255T1 (de) | Verfahren zur mikrobiologischen reinigung von wasser. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 0840746 Country of ref document: EP |
|
| REN | Ceased due to non-payment of the annual fee |