DE69633567D1 - Verfahren zur reinigung von proteinen - Google Patents

Verfahren zur reinigung von proteinen

Info

Publication number
DE69633567D1
DE69633567D1 DE69633567T DE69633567T DE69633567D1 DE 69633567 D1 DE69633567 D1 DE 69633567D1 DE 69633567 T DE69633567 T DE 69633567T DE 69633567 T DE69633567 T DE 69633567T DE 69633567 D1 DE69633567 D1 DE 69633567D1
Authority
DE
Germany
Prior art keywords
pct
sec
deionized water
date
cleaning method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69633567T
Other languages
English (en)
Other versions
DE69633567T2 (de
Inventor
Francois Fauquex
Catherine Georges
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Novartis AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis AG filed Critical Novartis AG
Application granted granted Critical
Publication of DE69633567D1 publication Critical patent/DE69633567D1/de
Publication of DE69633567T2 publication Critical patent/DE69633567T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
    • Y10S530/811—Peptides or proteins is immobilized on, or in, an inorganic carrier
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
    • Y10S530/812—Peptides or proteins is immobilized on, or in, an organic carrier

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69633567T 1995-07-26 1996-07-17 Verfahren zur reinigung von proteinen Expired - Fee Related DE69633567T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP95810485 1995-07-26
EP95810485 1995-07-26
PCT/EP1996/003144 WO1997005159A2 (en) 1995-07-26 1996-07-17 Method for the purification of proteins

Publications (2)

Publication Number Publication Date
DE69633567D1 true DE69633567D1 (de) 2004-11-11
DE69633567T2 DE69633567T2 (de) 2006-02-23

Family

ID=8221776

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69633567T Expired - Fee Related DE69633567T2 (de) 1995-07-26 1996-07-17 Verfahren zur reinigung von proteinen

Country Status (13)

Country Link
US (1) US5990289A (de)
EP (1) EP0840746B1 (de)
JP (1) JP3833255B2 (de)
AT (1) ATE278705T1 (de)
AU (1) AU6700796A (de)
CA (1) CA2226080A1 (de)
DE (1) DE69633567T2 (de)
DK (1) DK0840746T3 (de)
ES (1) ES2227603T3 (de)
PT (1) PT840746E (de)
TW (1) TW442492B (de)
WO (1) WO1997005159A2 (de)
ZA (1) ZA966315B (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6479300B1 (en) 1999-03-15 2002-11-12 Millipore Corporation Metal loaded ligand bound membranes for metal ion affinity chromatography
US7335383B2 (en) * 2001-01-16 2008-02-26 The Regents Of The University Of Michigan Generation of nitric oxide in vivo from nitrite, nitrate or nitrosothiols endogenous in blood
US7128904B2 (en) * 2001-01-16 2006-10-31 The Regents Of The University Of Michigan Material containing metal ion ligand complex producing nitric oxide in contact with blood
JP4283666B2 (ja) * 2001-06-21 2009-06-24 クローンテック ラボラトリーズ インク． 水溶性ポリマー金属イオンアフィニティ組成物、およびその使用方法
MXPA04005189A (es) 2001-11-28 2005-02-17 Sandoz Ag Purificacion cromatografica de eritropoyetina humana recombinante.
WO2006042263A2 (en) * 2004-10-12 2006-04-20 Clontech Laboratories, Inc. Phosphoprotein affinity resins and methods for making and using the same
US20080241208A1 (en) * 2005-06-30 2008-10-02 Charles Shanley Methods, Compositions and Devices For Promoting Anglogenesis
WO2007064895A2 (en) * 2005-12-02 2007-06-07 The Regents Of The University Of Michigan Polymer compositions, coatings and devices, and methods of making and using the same
SG191186A1 (en) 2010-12-15 2013-07-31 Baxter Int Eluate collection using conductivity gradient

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES8302778A1 (es) * 1980-11-10 1983-02-01 Genentech Inc Un procedimiento para producir un polipeptido antiviral.
EP0205404B1 (de) * 1985-06-11 1992-07-15 Ciba-Geigy Ag Hybrid-Interferone
CA1304886C (en) * 1986-07-10 1992-07-07 Heinz Dobeli Metal chelate resins
US5169936A (en) * 1989-04-14 1992-12-08 Biogen, Inc. Protein purification on immobilized metal affinity resins effected by elution using a weak ligand
US5136025A (en) * 1990-04-04 1992-08-04 California Biotechnology Inc. Method to purify basic fibroblast growth factor

Also Published As

Publication number Publication date
DE69633567T2 (de) 2006-02-23
JP3833255B2 (ja) 2006-10-11
JPH11509853A (ja) 1999-08-31
WO1997005159A2 (en) 1997-02-13
PT840746E (pt) 2004-12-31
CA2226080A1 (en) 1997-02-13
ATE278705T1 (de) 2004-10-15
ZA966315B (en) 1997-01-27
AU6700796A (en) 1997-02-26
WO1997005159A3 (en) 1997-03-06
US5990289A (en) 1999-11-23
ES2227603T3 (es) 2005-04-01
DK0840746T3 (da) 2004-11-29
EP0840746A2 (de) 1998-05-13
EP0840746B1 (de) 2004-10-06
TW442492B (en) 2001-06-23

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee