ZA966151B - Salt mediated size exclusion chromatography for buffer exchange and protein refolding. - Google Patents

Salt mediated size exclusion chromatography for buffer exchange and protein refolding.

Info

Publication number
ZA966151B
ZA966151B ZA9606151A ZA966151A ZA966151B ZA 966151 B ZA966151 B ZA 966151B ZA 9606151 A ZA9606151 A ZA 9606151A ZA 966151 A ZA966151 A ZA 966151A ZA 966151 B ZA966151 B ZA 966151B
Authority
ZA
South Africa
Prior art keywords
size exclusion
exclusion chromatography
buffer exchange
protein refolding
salt mediated
Prior art date
Application number
ZA9606151A
Other languages
English (en)
Inventor
Robert J Seely
Michael R Ladisch
Original Assignee
Amgen Inc & Purdue Res Foundat
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc & Purdue Res Foundat filed Critical Amgen Inc & Purdue Res Foundat
Publication of ZA966151B publication Critical patent/ZA966151B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/48—Nerve growth factor [NGF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
ZA9606151A 1995-07-21 1996-07-19 Salt mediated size exclusion chromatography for buffer exchange and protein refolding. ZA966151B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US50542095A 1995-07-21 1995-07-21

Publications (1)

Publication Number Publication Date
ZA966151B true ZA966151B (en) 1997-02-04

Family

ID=24010237

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA9606151A ZA966151B (en) 1995-07-21 1996-07-19 Salt mediated size exclusion chromatography for buffer exchange and protein refolding.

Country Status (6)

Country Link
EP (1) EP0840745A2 (xx)
JP (1) JPH11509551A (xx)
AU (1) AU707719B2 (xx)
CA (1) CA2227318C (xx)
WO (1) WO1997004003A2 (xx)
ZA (1) ZA966151B (xx)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1627920A1 (en) * 2000-01-24 2006-02-22 Polymun Scientific Immunbiologische Forschung GmbH Method for the manufacture of recombinant trypsin
JP5229730B2 (ja) * 2008-10-07 2013-07-03 独立行政法人農業・食品産業技術総合研究機構 固相合成装置用反応槽モジュールおよびそれを用いた固相合成装置

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
US4677196A (en) * 1985-09-06 1987-06-30 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US4705848A (en) * 1986-06-02 1987-11-10 International Minerals & Chemical Corp. Isolation of bioactive, monomeric growth hormone
DE3618817A1 (de) * 1986-06-04 1987-12-10 Behringwerke Ag Verfahren zur gewinnung aktiver proteine aus einer biologisch inaktiven form
ATE156491T1 (de) * 1987-05-11 1997-08-15 Chiron Corp Prozess zur gewinnung von gereinigtem, oxidiertem, renaturiertem, rekombinantem interleukin-2 aus mikroorganismen
DK580789D0 (da) * 1989-11-20 1989-11-20 Novo Nordisk As Fremgangsmaade til oprensning af polypeptider
DK0657466T3 (da) * 1993-11-10 1997-07-14 Pfizer Fremgangsmåde til refoldning af (pro-)chymosin, som omfatter recirkulering af urea

Also Published As

Publication number Publication date
AU6544496A (en) 1997-02-18
CA2227318A1 (en) 1997-02-06
JPH11509551A (ja) 1999-08-24
AU707719B2 (en) 1999-07-15
MX9800520A (es) 1998-05-31
CA2227318C (en) 2001-09-25
WO1997004003A2 (en) 1997-02-06
WO1997004003A3 (en) 1997-02-27
EP0840745A2 (en) 1998-05-13

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