CA2227318A1 - Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salt - Google Patents

Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salt

Info

Publication number
CA2227318A1
CA2227318A1 CA002227318A CA2227318A CA2227318A1 CA 2227318 A1 CA2227318 A1 CA 2227318A1 CA 002227318 A CA002227318 A CA 002227318A CA 2227318 A CA2227318 A CA 2227318A CA 2227318 A1 CA2227318 A1 CA 2227318A1
Authority
CA
Canada
Prior art keywords
stationary phase
salt
protein
buffer exchange
protein refolding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002227318A
Other languages
French (fr)
Other versions
CA2227318C (en
Inventor
Robert J. Seely
Michael R. Ladisch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Purdue Research Foundation
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2227318A1 publication Critical patent/CA2227318A1/en
Application granted granted Critical
Publication of CA2227318C publication Critical patent/CA2227318C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/48—Nerve growth factor [NGF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Disclosed is a quick and efficient refolding process which utilizes cellulosic rolled stationary phase to rapidly separate the reduced, denatured protein from the denaturant solution, thereby promoting high protein refold yields at higher protein concentrations, while significantly decreasing the volume needed to achieve protein refolding.
CA002227318A 1995-07-21 1996-07-11 Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salt Expired - Fee Related CA2227318C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US50542095A 1995-07-21 1995-07-21
US08/505,420 1995-07-21
PCT/US1996/011581 WO1997004003A2 (en) 1995-07-21 1996-07-11 Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salts

Publications (2)

Publication Number Publication Date
CA2227318A1 true CA2227318A1 (en) 1997-02-06
CA2227318C CA2227318C (en) 2001-09-25

Family

ID=24010237

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002227318A Expired - Fee Related CA2227318C (en) 1995-07-21 1996-07-11 Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salt

Country Status (6)

Country Link
EP (1) EP0840745A2 (en)
JP (1) JPH11509551A (en)
AU (1) AU707719B2 (en)
CA (1) CA2227318C (en)
WO (1) WO1997004003A2 (en)
ZA (1) ZA966151B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1627920A1 (en) * 2000-01-24 2006-02-22 Polymun Scientific Immunbiologische Forschung GmbH Method for the manufacture of recombinant trypsin
JP5229730B2 (en) * 2008-10-07 2013-07-03 独立行政法人農業・食品産業技術総合研究機構 Reactor module for solid-phase synthesizer and solid-phase synthesizer using the same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
US4677196A (en) * 1985-09-06 1987-06-30 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US4705848A (en) * 1986-06-02 1987-11-10 International Minerals & Chemical Corp. Isolation of bioactive, monomeric growth hormone
DE3618817A1 (en) * 1986-06-04 1987-12-10 Behringwerke Ag METHOD FOR OBTAINING ACTIVE PROTEINS FROM A BIOLOGICALLY INACTIVE FORM
ATE156491T1 (en) * 1987-05-11 1997-08-15 Chiron Corp PROCESS FOR OBTAINING PURIFIED, OXIDIZED, RENatured, RECOMBINANT INTERLEUKIN-2 FROM MICROORGANISMS
DK580789D0 (en) * 1989-11-20 1989-11-20 Novo Nordisk As PROCEDURE FOR PURIFICATION OF POLYPEPTIDES
DE69401490T2 (en) * 1993-11-10 1997-04-30 Pfizer Process for the refolding of (pro-) chymosin, with recycling of urea

Also Published As

Publication number Publication date
WO1997004003A3 (en) 1997-02-27
AU6544496A (en) 1997-02-18
CA2227318C (en) 2001-09-25
WO1997004003A2 (en) 1997-02-06
JPH11509551A (en) 1999-08-24
MX9800520A (en) 1998-05-31
EP0840745A2 (en) 1998-05-13
AU707719B2 (en) 1999-07-15
ZA966151B (en) 1997-02-04

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Legal Events

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