CA2227318A1 - Procede de repliement de proteines par echange de tampons utilisant une phase stationnaire continue capable de separer des proteines d'un sel - Google Patents

Procede de repliement de proteines par echange de tampons utilisant une phase stationnaire continue capable de separer des proteines d'un sel

Info

Publication number
CA2227318A1
CA2227318A1 CA002227318A CA2227318A CA2227318A1 CA 2227318 A1 CA2227318 A1 CA 2227318A1 CA 002227318 A CA002227318 A CA 002227318A CA 2227318 A CA2227318 A CA 2227318A CA 2227318 A1 CA2227318 A1 CA 2227318A1
Authority
CA
Canada
Prior art keywords
stationary phase
salt
protein
buffer exchange
protein refolding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002227318A
Other languages
English (en)
Other versions
CA2227318C (fr
Inventor
Robert J. Seely
Michael R. Ladisch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Purdue Research Foundation
Original Assignee
Amgen Inc.
Purdue Research Foundation
Robert J. Seely
Michael R. Ladisch
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc., Purdue Research Foundation, Robert J. Seely, Michael R. Ladisch filed Critical Amgen Inc.
Publication of CA2227318A1 publication Critical patent/CA2227318A1/fr
Application granted granted Critical
Publication of CA2227318C publication Critical patent/CA2227318C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/48—Nerve growth factor [NGF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
CA002227318A 1995-07-21 1996-07-11 Procede de repliement de proteines par echange de tampons utilisant une phase stationnaire continue capable de separer des proteines d'un sel Expired - Fee Related CA2227318C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US50542095A 1995-07-21 1995-07-21
US08/505,420 1995-07-21
PCT/US1996/011581 WO1997004003A2 (fr) 1995-07-21 1996-07-11 Procede de repliement de proteines par echange de tampons utilisant une phase stationnaire continue capable de separer des proteines d'un sel

Publications (2)

Publication Number Publication Date
CA2227318A1 true CA2227318A1 (fr) 1997-02-06
CA2227318C CA2227318C (fr) 2001-09-25

Family

ID=24010237

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002227318A Expired - Fee Related CA2227318C (fr) 1995-07-21 1996-07-11 Procede de repliement de proteines par echange de tampons utilisant une phase stationnaire continue capable de separer des proteines d'un sel

Country Status (6)

Country Link
EP (1) EP0840745A2 (fr)
JP (1) JPH11509551A (fr)
AU (1) AU707719B2 (fr)
CA (1) CA2227318C (fr)
WO (1) WO1997004003A2 (fr)
ZA (1) ZA966151B (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1627920A1 (fr) * 2000-01-24 2006-02-22 Polymun Scientific Immunbiologische Forschung GmbH Procédé pour la production de trypsine de recombinaison
JP5229730B2 (ja) * 2008-10-07 2013-07-03 独立行政法人農業・食品産業技術総合研究機構 固相合成装置用反応槽モジュールおよびそれを用いた固相合成装置

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
US4677196A (en) * 1985-09-06 1987-06-30 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US4705848A (en) * 1986-06-02 1987-11-10 International Minerals & Chemical Corp. Isolation of bioactive, monomeric growth hormone
DE3618817A1 (de) * 1986-06-04 1987-12-10 Behringwerke Ag Verfahren zur gewinnung aktiver proteine aus einer biologisch inaktiven form
DE3855986T2 (de) * 1987-05-11 1998-02-05 Chiron Corp Prozess zur gewinnung von gereinigtem, oxidiertem, renaturiertem, rekombinantem interleukin-2 aus mikroorganismen
DK580789D0 (da) * 1989-11-20 1989-11-20 Novo Nordisk As Fremgangsmaade til oprensning af polypeptider
DE69401490T2 (de) * 1993-11-10 1997-04-30 Pfizer Prozess zur Rückfaltung von (pro-)Chymosin, unter Rückführung von Harnstoff

Also Published As

Publication number Publication date
AU6544496A (en) 1997-02-18
CA2227318C (fr) 2001-09-25
WO1997004003A2 (fr) 1997-02-06
AU707719B2 (en) 1999-07-15
ZA966151B (en) 1997-02-04
MX9800520A (es) 1998-05-31
JPH11509551A (ja) 1999-08-24
WO1997004003A3 (fr) 1997-02-27
EP0840745A2 (fr) 1998-05-13

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Legal Events

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