WO1997004003A3 - Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salts - Google Patents
Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salts Download PDFInfo
- Publication number
- WO1997004003A3 WO1997004003A3 PCT/US1996/011581 US9611581W WO9704003A3 WO 1997004003 A3 WO1997004003 A3 WO 1997004003A3 US 9611581 W US9611581 W US 9611581W WO 9704003 A3 WO9704003 A3 WO 9704003A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- stationary phase
- salts
- protein
- buffer exchange
- protein refolding
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/48—Nerve growth factor [NGF]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Peptides Or Proteins (AREA)
Abstract
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP96925295A EP0840745A2 (en) | 1995-07-21 | 1996-07-11 | Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salt |
AU65444/96A AU707719B2 (en) | 1995-07-21 | 1996-07-11 | Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salt |
JP9506744A JPH11509551A (en) | 1995-07-21 | 1996-07-11 | A method for unwinding proteins by buffer exchange using a continuous stationary phase capable of separating proteins from salts |
CA002227318A CA2227318C (en) | 1995-07-21 | 1996-07-11 | Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salt |
MXPA/A/1998/000520A MXPA98000520A (en) | 1995-07-21 | 1998-01-16 | Process for reploying proteins through shock exchange using a continuous stationary phase capable of separating the proteins of the |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US50542095A | 1995-07-21 | 1995-07-21 | |
US08/505,420 | 1995-07-21 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO1997004003A2 WO1997004003A2 (en) | 1997-02-06 |
WO1997004003A3 true WO1997004003A3 (en) | 1997-02-27 |
Family
ID=24010237
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1996/011581 WO1997004003A2 (en) | 1995-07-21 | 1996-07-11 | Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salts |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0840745A2 (en) |
JP (1) | JPH11509551A (en) |
AU (1) | AU707719B2 (en) |
CA (1) | CA2227318C (en) |
WO (1) | WO1997004003A2 (en) |
ZA (1) | ZA966151B (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1627920A1 (en) * | 2000-01-24 | 2006-02-22 | Polymun Scientific Immunbiologische Forschung GmbH | Method for the manufacture of recombinant trypsin |
JP5229730B2 (en) * | 2008-10-07 | 2013-07-03 | 独立行政法人農業・食品産業技術総合研究機構 | Reactor module for solid-phase synthesizer and solid-phase synthesizer using the same |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0218374A1 (en) * | 1985-09-06 | 1987-04-15 | International Minerals And Chemical Corporation | Purification and activation of proteins from insoluble inclusion bodies |
US4705848A (en) * | 1986-06-02 | 1987-11-10 | International Minerals & Chemical Corp. | Isolation of bioactive, monomeric growth hormone |
WO1988008849A1 (en) * | 1987-05-11 | 1988-11-17 | Cetus Corporation | Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms |
US4965344A (en) * | 1986-06-04 | 1990-10-23 | Behringwerke Aktiengesellschaft | Process for obtaining active proteins from a biologically inactive form |
US4977248A (en) * | 1985-03-29 | 1990-12-11 | Creighton Thomas E | Process for the production of a protein |
WO1991007428A1 (en) * | 1989-11-20 | 1991-05-30 | Novo Nordisk A/S | A protein purification method |
EP0657466A1 (en) * | 1993-11-10 | 1995-06-14 | Pfizer Inc. | A process for refolding of (pro-)chymosin, comprising recycling of urea |
-
1996
- 1996-07-11 EP EP96925295A patent/EP0840745A2/en not_active Withdrawn
- 1996-07-11 CA CA002227318A patent/CA2227318C/en not_active Expired - Fee Related
- 1996-07-11 JP JP9506744A patent/JPH11509551A/en active Pending
- 1996-07-11 WO PCT/US1996/011581 patent/WO1997004003A2/en not_active Application Discontinuation
- 1996-07-11 AU AU65444/96A patent/AU707719B2/en not_active Ceased
- 1996-07-19 ZA ZA9606151A patent/ZA966151B/en unknown
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4977248A (en) * | 1985-03-29 | 1990-12-11 | Creighton Thomas E | Process for the production of a protein |
EP0218374A1 (en) * | 1985-09-06 | 1987-04-15 | International Minerals And Chemical Corporation | Purification and activation of proteins from insoluble inclusion bodies |
US4677196A (en) * | 1985-09-06 | 1987-06-30 | International Minerals & Chemical Corp. | Purification and activation of proteins from insoluble inclusion bodies |
US4705848A (en) * | 1986-06-02 | 1987-11-10 | International Minerals & Chemical Corp. | Isolation of bioactive, monomeric growth hormone |
US4965344A (en) * | 1986-06-04 | 1990-10-23 | Behringwerke Aktiengesellschaft | Process for obtaining active proteins from a biologically inactive form |
WO1988008849A1 (en) * | 1987-05-11 | 1988-11-17 | Cetus Corporation | Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms |
WO1991007428A1 (en) * | 1989-11-20 | 1991-05-30 | Novo Nordisk A/S | A protein purification method |
EP0657466A1 (en) * | 1993-11-10 | 1995-06-14 | Pfizer Inc. | A process for refolding of (pro-)chymosin, comprising recycling of urea |
Non-Patent Citations (1)
Title |
---|
HAMAKER K.H. ET AL.: "Chromatography for rapid buffer exchange and refolding of secretory leukocyte protease inhibitor", BIOTECHNOLOGY PROGRESS, vol. 12, no. 2, 1996, pages 184 - 189, XP000610750 * |
Also Published As
Publication number | Publication date |
---|---|
WO1997004003A2 (en) | 1997-02-06 |
MX9800520A (en) | 1998-05-31 |
AU6544496A (en) | 1997-02-18 |
ZA966151B (en) | 1997-02-04 |
AU707719B2 (en) | 1999-07-15 |
EP0840745A2 (en) | 1998-05-13 |
CA2227318C (en) | 2001-09-25 |
CA2227318A1 (en) | 1997-02-06 |
JPH11509551A (en) | 1999-08-24 |
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