ATE147753T1 - Prozess zur rückfaltung von (pro-)chymosin, unter rückführung von harnstoff - Google Patents

Prozess zur rückfaltung von (pro-)chymosin, unter rückführung von harnstoff

Info

Publication number
ATE147753T1
ATE147753T1 AT94307839T AT94307839T ATE147753T1 AT E147753 T1 ATE147753 T1 AT E147753T1 AT 94307839 T AT94307839 T AT 94307839T AT 94307839 T AT94307839 T AT 94307839T AT E147753 T1 ATE147753 T1 AT E147753T1
Authority
AT
Austria
Prior art keywords
chymosin
refolding
recirculation
urea
pro
Prior art date
Application number
AT94307839T
Other languages
English (en)
Inventor
James G Crawford
Stanley R Stober
Original Assignee
Pfizer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer filed Critical Pfizer
Application granted granted Critical
Publication of ATE147753T1 publication Critical patent/ATE147753T1/de

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/145—Ultrafiltration
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Water Supply & Treatment (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Peptides Or Proteins (AREA)
  • Cephalosporin Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
AT94307839T 1993-11-10 1994-10-25 Prozess zur rückfaltung von (pro-)chymosin, unter rückführung von harnstoff ATE147753T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US15063293A 1993-11-10 1993-11-10

Publications (1)

Publication Number Publication Date
ATE147753T1 true ATE147753T1 (de) 1997-02-15

Family

ID=22535379

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94307839T ATE147753T1 (de) 1993-11-10 1994-10-25 Prozess zur rückfaltung von (pro-)chymosin, unter rückführung von harnstoff

Country Status (7)

Country Link
EP (1) EP0657466B1 (de)
JP (1) JP2749269B2 (de)
AT (1) ATE147753T1 (de)
DE (1) DE69401490T2 (de)
DK (1) DK0657466T3 (de)
ES (1) ES2096416T3 (de)
GR (1) GR3022653T3 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997004003A2 (en) * 1995-07-21 1997-02-06 Amgen Inc. Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salts
JP2000281638A (ja) 1999-03-26 2000-10-10 Snow Brand Milk Prod Co Ltd 尿素の分離方法
HU223808B1 (hu) * 1999-03-26 2005-02-28 Snow Brand Milk Products Co., Ltd. Eljárás karbamid izolálására, karbamidkompozíciók és eljárás az előállításukra
EP1627920A1 (de) * 2000-01-24 2006-02-22 Polymun Scientific Immunbiologische Forschung GmbH Verfahren zur Herstellung von rekombinantem Trypsin
WO2002036752A2 (en) 2000-11-06 2002-05-10 Chr. Hansen A/S Method of producing non-bovine chymosin and use thereof
US20050092664A1 (en) * 2003-11-05 2005-05-05 Ghosh Pushpito K. Improvised device for concentrating the aqueous solution and a pocess thereof
WO2011005488A1 (en) 2009-06-22 2011-01-13 Amgen Inc. Refolding proteins using a chemically controlled redox state
JP2012531428A (ja) 2009-06-25 2012-12-10 アムジエン・インコーポレーテツド 哺乳類以外の系で発現されるタンパク質の捕獲精製プロセス
CN103211035B (zh) * 2013-04-12 2014-11-05 江南大学 一种液态型凝乳酶制剂的制备方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD146751A3 (de) * 1980-02-22 1981-03-04 Lothar Guenther Verfahren zur aufarbeitung von prozessabwasser der harnstoffherstellung

Also Published As

Publication number Publication date
EP0657466A1 (de) 1995-06-14
ES2096416T3 (es) 1997-03-01
DE69401490T2 (de) 1997-04-30
DK0657466T3 (da) 1997-07-14
DE69401490D1 (de) 1997-02-27
JP2749269B2 (ja) 1998-05-13
EP0657466B1 (de) 1997-01-15
JPH07184650A (ja) 1995-07-25
GR3022653T3 (en) 1997-05-31

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EEIH Change in the person of patent owner
REN Ceased due to non-payment of the annual fee