ATE147753T1 - Prozess zur rückfaltung von (pro-)chymosin, unter rückführung von harnstoff - Google Patents
Prozess zur rückfaltung von (pro-)chymosin, unter rückführung von harnstoffInfo
- Publication number
- ATE147753T1 ATE147753T1 AT94307839T AT94307839T ATE147753T1 AT E147753 T1 ATE147753 T1 AT E147753T1 AT 94307839 T AT94307839 T AT 94307839T AT 94307839 T AT94307839 T AT 94307839T AT E147753 T1 ATE147753 T1 AT E147753T1
- Authority
- AT
- Austria
- Prior art keywords
- chymosin
- refolding
- recirculation
- urea
- pro
- Prior art date
Links
- 108090000746 Chymosin Proteins 0.000 title 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 title 1
- 239000004202 carbamide Substances 0.000 title 1
- 229940080701 chymosin Drugs 0.000 title 1
- GNOLWGAJQVLBSM-UHFFFAOYSA-N n,n,5,7-tetramethyl-1,2,3,4-tetrahydronaphthalen-1-amine Chemical compound C1=C(C)C=C2C(N(C)C)CCCC2=C1C GNOLWGAJQVLBSM-UHFFFAOYSA-N 0.000 title 1
- 238000002955 isolation Methods 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000000108 ultra-filtration Methods 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/145—Ultrafiltration
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
- C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Water Supply & Treatment (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Peptides Or Proteins (AREA)
- Cephalosporin Compounds (AREA)
- Saccharide Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15063293A | 1993-11-10 | 1993-11-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE147753T1 true ATE147753T1 (de) | 1997-02-15 |
Family
ID=22535379
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT94307839T ATE147753T1 (de) | 1993-11-10 | 1994-10-25 | Prozess zur rückfaltung von (pro-)chymosin, unter rückführung von harnstoff |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0657466B1 (de) |
| JP (1) | JP2749269B2 (de) |
| AT (1) | ATE147753T1 (de) |
| DE (1) | DE69401490T2 (de) |
| DK (1) | DK0657466T3 (de) |
| ES (1) | ES2096416T3 (de) |
| GR (1) | GR3022653T3 (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997004003A2 (en) * | 1995-07-21 | 1997-02-06 | Amgen Inc. | Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salts |
| JP2000281638A (ja) | 1999-03-26 | 2000-10-10 | Snow Brand Milk Prod Co Ltd | 尿素の分離方法 |
| HU223808B1 (hu) * | 1999-03-26 | 2005-02-28 | Snow Brand Milk Products Co., Ltd. | Eljárás karbamid izolálására, karbamidkompozíciók és eljárás az előállításukra |
| EP1627920A1 (de) * | 2000-01-24 | 2006-02-22 | Polymun Scientific Immunbiologische Forschung GmbH | Verfahren zur Herstellung von rekombinantem Trypsin |
| AU2002213838A1 (en) | 2000-11-06 | 2002-05-15 | Chr. Hansen A/S | Method of producing non-bovine chymosin and use thereof |
| US20050092664A1 (en) * | 2003-11-05 | 2005-05-05 | Ghosh Pushpito K. | Improvised device for concentrating the aqueous solution and a pocess thereof |
| KR101741859B1 (ko) | 2009-06-22 | 2017-06-15 | 암젠 인크 | 화학적으로 조절된 산화환원 상태를 사용한 단백질의 재폴딩 |
| SI3660032T1 (sl) | 2009-06-25 | 2026-02-27 | Amgen Inc. | Postopki zajemanja in čiščenja beljakovin, izraženih v nesesalskem sistemu |
| CN103211035B (zh) * | 2013-04-12 | 2014-11-05 | 江南大学 | 一种液态型凝乳酶制剂的制备方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DD146751A3 (de) * | 1980-02-22 | 1981-03-04 | Lothar Guenther | Verfahren zur aufarbeitung von prozessabwasser der harnstoffherstellung |
-
1994
- 1994-10-25 DK DK94307839.4T patent/DK0657466T3/da active
- 1994-10-25 DE DE69401490T patent/DE69401490T2/de not_active Expired - Fee Related
- 1994-10-25 ES ES94307839T patent/ES2096416T3/es not_active Expired - Lifetime
- 1994-10-25 AT AT94307839T patent/ATE147753T1/de not_active IP Right Cessation
- 1994-10-25 EP EP94307839A patent/EP0657466B1/de not_active Expired - Lifetime
- 1994-11-04 JP JP6271536A patent/JP2749269B2/ja not_active Expired - Lifetime
-
1997
- 1997-02-26 GR GR970400340T patent/GR3022653T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DK0657466T3 (da) | 1997-07-14 |
| GR3022653T3 (en) | 1997-05-31 |
| JP2749269B2 (ja) | 1998-05-13 |
| DE69401490D1 (de) | 1997-02-27 |
| JPH07184650A (ja) | 1995-07-25 |
| DE69401490T2 (de) | 1997-04-30 |
| EP0657466B1 (de) | 1997-01-15 |
| ES2096416T3 (es) | 1997-03-01 |
| EP0657466A1 (de) | 1995-06-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| EEIH | Change in the person of patent owner | ||
| REN | Ceased due to non-payment of the annual fee |